共查询到20条相似文献,搜索用时 31 毫秒
1.
Volker Patzel Ralf Kronenwett Karola Rittner Georg Sczakiel 《Nucleosides, nucleotides & nucleic acids》2013,32(5-6):711-715
Abstract Fast annealing of complementary RNA in vitro is related to effective antisense RNA-mediated regulation of gene expression and inhibition of viral replication in living cells. Pools of antisense RNA can be selected for species that anneal fast with a given target strand. In this study, we compare the technical and biological advantages and disadvantages of a single round selection assay with extensive multiple cycle selection for fast-annealing antisense RNA species. 相似文献
2.
《Nucleosides, nucleotides & nucleic acids》2013,32(5-8):1607-1609
Abstract Full-length and 4 nucleotides truncated Locked Nucleic Acid (LNA) modifications of ISIS 3521 were compared for antisense properties in a cellular assay. ISIS 3521 is a 20-mer phosphorothioate designed to hybridise to human protein kinase C-α (PKC-α) mRNA and is currently submitted to clinical trials against cancer. We report that LNA can potentate this antisense oligo and retain the antisense potential with shorter oligos. 相似文献
3.
《Nucleosides, nucleotides & nucleic acids》2013,32(5-8):1631-1634
Abstract Different phenylalkyl backbone modified antisense oligonucleotides complementary to the Hepatitis C virus (HCV) RNA nucleotides 326–342 were synthesized. The lipohilic character of modified oligonucleotides was determined from RP-HPLC retention times. The inhibitory effect of these antisense oligonucleotides on HCV gene expression was analyzed in an in vitro test system. 相似文献
4.
Markus W. Gerrnann James M. Aramini Bernd W. Kalisch Richard T. Pon Johan H. van de Sande 《Nucleosides, nucleotides & nucleic acids》2013,32(7-9):1481-1485
Abstract Oligodeoxynucleotides that possess alpha anomeric nucleotides and polarity reversals show promise for application in the area of antisense therapy. Here we provide a survey of the spectroscopic, thermodynamic, and enzymatic techniques used in our laboratories to investigate model systems containing such unnatural features with the ultimate goal of designing a new class of more potent and effective antisense therapeutics. 相似文献
5.
Abstract Two dinucleoside monophosphate analogues containing disulfide linkages (1 and 2) have been prepared for incorporation into oligonucleotides. The modified oligomers will be tested for their potential as antisense agents. 相似文献
6.
Karl-Heinz Altmann Pierre Martin Nicholas M. Dean Brett P. Mania 《Nucleosides, nucleotides & nucleic acids》2013,32(7-9):917-926
Abstract 6′-substituted carbocyclic deoxyribonucleosides and 2′-O-ethylene glycol substituted ribonucleosides have been evaluated as building blocks for antisense oligonucleotides. Within the former class 6′-hydroxy substituted building blocks in combination with internucleoside phosphorothioate linkages have the potential to enhance antisense activity. 2′-O-ethylene glycol substituted ribonucleosides generally allow for the construction of potent antisense oligonucleotides with reduced phosphorothioate content, but differences exist in their effects on biological activity in cell culture in spite of virtually identical effects on RNA-binding affinity. Activity enhancement was most pronounced for a 2′-O-methoxyethyl substituent. 相似文献
7.
Richard V. Giles David G. Spiller Richard E. Clark David M. Tidd 《Nucleosides, nucleotides & nucleic acids》2013,32(9):1935-1944
Abstract A region of c-myc mRNA was identified which permitted very efficient antisense effects to be achieved in living cells using chimeric methylphosphonate-phosphodiester antisense effectors. Novel inosine—containing ribozymes (which cleave after NCH triplets) were directed to an ACA triplet within this region and delivered into living cells. No ribozyme intracellular activity could be identified. Very low ribozyme function was also observed in in vitro assays using a 1700nt substrate RNA. 相似文献
8.
P. Dan Cook 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1141-1162
Abstract I provide a brief review and perspective thoughts concerning the antisense oligonucleotide, drug discovery paradigm. 相似文献
9.
I. Robbins J. E. Gee G. Mitta B. Rayner S. Vichier-guerre M. Leng 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1667-1668
Abstract Most second-generation ON antisense analogs do not activate RNase H. Alternative strategies to arrest translation in a reticulocyte cell-free assay programmed by VSV mRNAs have been explored. 相似文献
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11.
A. Peyman H. Ragg T. Ulshöfer D. W. Will E. Uhlmann 《Nucleosides, nucleotides & nucleic acids》2013,32(7-9):1215-1219
Abstract The sequence-specificity of antisense oligonucleotides (ODN) against c-myc mRNA was tested by Northern blot analysis. Rat smooth muscle cells were treated with antisense or control ODN against c-myc modified by the “minimal protection strategy”. At 0.3 μM concentration the ODN show a very specific reduction in c-myc mRNA levels. Use of the “minimal protection strategy” minimizes nonspecific effects as observed for all-phosphorothioate ODN containing four consecutive guanine residues. 相似文献
12.
《Nucleosides, nucleotides & nucleic acids》2013,32(5-8):1611-1613
Abstract Peptide nucleic acids (PNA) are promising antisense molecule for blocking gene expression in cell culture or in vivo. Nevertheless because they are poor efficient to pass the cellular membrane, it is necessary to use a vectorisation agent to observe an inhibitory effect. We describe the coupling of the rhodamine labeled 17-mer antisense PNA to a fusogenic peptide from antenapedia via S-S linkage, the studies of the penetration of this complex into fibroblast cells and its inhibitory effect on piml targeted protononcogene. 相似文献
13.
Valérie Hélin Marina Gottikh Zohar Mishal Frédéric Subra Claude Malvy Marc Lavignon 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1271-1276
Abstract We developed a rapid screening system, using two reporter genes under the control of the same promoter, to identify the biological activity of modified or/and vectorized antisense oligodeoxynucleotides (ODNs). The ability of a dendrimeric structure and a monocationic cholesterol derivative to enhance ODN cellular uptake was previously investigated by fluorescence analysis. Then, the assay system was validated through investigating the effect of both vectors on antisense ODN efficiency. 相似文献
14.
《Nucleosides, nucleotides & nucleic acids》2013,32(5-8):1619-1621
Abstract ENATM antisense oligonucleotides for vascular endothelial growth factor (VEGF) mRNA were synthesized and evaluated in A549 lung cancer cells. It was found that the VEGF ENA-antisense inhibited not only the expression of VEGF, but also the expression of three genes, which were found in Genbank by BLAST and Clustal W search and considered likely to bind to the VEGF ENA-antisense. These results indicate that ENA-antisense oligonucleotides act in a sequence-specific manner, and could be used as effective antisense drugs. 相似文献
15.
Ekambar R. Kandimalla Jamal Temsamani Sudhir Agrawal 《Nucleosides, nucleotides & nucleic acids》2013,32(3-5):1031-1035
Abstract 2′-O-methylribonucleoside methylphosphonamidites are synthesized and incorporated into oligonucleotides to obtain chimeric antisense oligonucleotides. The resulting oligonucleotide binds to their target RNA/DNA sequences efficiently and stable in a medium containing bovine serum. 相似文献
16.
Hideo Hosaka Yoshikazu Suzuki Hiroyuki Nakamura Hidenori Funakoshi Hideki Nakashima Naoki Yamamoto 《Nucleosides, nucleotides & nucleic acids》2013,32(2-4):669-678
Abstract The deoxyribonucleoside-3′-yl O-bis(1,1,1,3,3,3-hexafluoro-2-propyl) phosphite units (3) could be converted into the O-nucleosidyl phosphonate, O-2-cyanoethyl O-nucleosidyl phosphonate, and O-1,1,1,3,3,3-hexafluoro-2-propyl O-nucleosidyl phosphonothioate. Compound 3a was activated by methylimidazole to give the dithymidylate derivatives (8). The appropriately protected nucleosidyl phosphonates (3) were applied to the synthesis of oligodeoxyribonucleotides used as antisense oligonucleotides. 相似文献
17.
Martin K. Bijsterbosch Muthiah Manoharan Kathleen L. Tivel Erik T. Rump Erik A.L. Biessen Remco L.A. De Vrueh 《Nucleosides, nucleotides & nucleic acids》2013,32(7-9):1165-1168
Abstract A cholesterol-conjugated phosphorothioate ICAM-1 antisense oligo-nucleotide was evaluated for its binding to lipoproteins and its biodistribution. Our study indicates that the conjugate behaves differently from the parent compound. 相似文献
18.
E. G. H. Wagner C. Persson M. Öhman K. Nordstrom 《Nucleosides, nucleotides & nucleic acids》2013,32(5-6):559-564
Abstract This paper gives a short summary of some approaches to investigate antisense RNA control in general, exemplified by studies of a particular model system - replication control of plasmid R1. 相似文献
19.
Balkrishen Bhat Guity Balow Andrei Guzaev P. Dan Cook Muthiah Manoharan 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1471-1472
Abstract A novel synthesis of the nucleoside-folic acid conjugates has been accomplished. This approach allowed us to synthesize several analogs, which were converted to phosphoramidites and successfully incorporated into therapeutically active antisense oligonucleotides. 相似文献
20.
Muthiah Manoharan Kathleen L. Tivel Laura K. Andrade V. Mohan Thomas P. Condon C. Frank Bennett 《Nucleosides, nucleotides & nucleic acids》2013,32(3-5):969-973
Abstract Cholic acid, cholesterol, several polyamines and polyethylene glycols were conjugated to antisense oligonucleotides targeted to human or murine intercellular adhesion molecule-1 (ICAM-1) mRNA to study their effects on cellular absorption. 相似文献