共查询到20条相似文献,搜索用时 15 毫秒
1.
Joseph Deutsch Sason Cohen Moshe Weisz Jehoshua Katzhendler 《Nucleosides, nucleotides & nucleic acids》2013,32(3):325-330
Abstract A simple and efficient synthesis of a solid support with a long chain polyamide spacer has been developed. The spacer was made by successive addition of ethylene-diamine and succinic anhydride. The obtained solid support gives very homogeneous 20 mer oligodeoxyribonucleotides, detected by HPLC and electrophoresis. 相似文献
2.
J. S. Debear J. A. Hayes M. P. Koleck G. R. Gough 《Nucleosides, nucleotides & nucleic acids》2013,32(5):821-830
Abstract A single type of controlled pore glass derivatized with 3-anisoyl-2′(3′)-O-benzoyluridine 5′-O-succinyl residues can be used as the support in solid phase syntheses of either oligoribo- or oligodeoxyribonucleotides. 相似文献
3.
《Nucleosides, nucleotides & nucleic acids》2013,32(5-8):1403-1406
Abstract A novel photocleavable universal support for the automated solid phase synthesis of oligonucleotides is described. The linker between the growing oligonucleotide chain and CPG support contains a nucleophilic amine protected with a photocleavable group. On exposure to UV light, this group is detached and the free amine affords cleavage of the oligonucleotide from the support. The use of long wavelength UV light avoids damage to the DNA. 相似文献
4.
C. Minard-basquin C. Chaix C. Pichot B. Mandrand 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1285-1287
Abstract A new strategy has been developed to obtain polymer-ODN conjugates. However, free ODN appeared to contaminate synthesis. Various hypotheses are described herein to explain this side reaction. 相似文献
5.
Abstract New variant of the manual method of oligonucleotide synthesis by “in situ” P(III) methodology will be presented. 相似文献
6.
Mr Andrey Yagodkin Mr Janne Weisel Professor Alex Azhayev 《Nucleosides, nucleotides & nucleic acids》2013,32(7-8):475-489
The universal solid support, USIII, representing a new and improved version of commercial USII, as well as 2 ′-deoxynucleoside and 2 ′-deoxy-2 ′-fluoronucleoside bound supports, incorporating a labile phenoxyacetyl fragment, was synthesized by an aminomethyl polystyrene carbamoylation with corresponding azides in the presence of aqueous triethylammonium bicarbonate. All three solid phases incorporate a stable urea tether, thus bridging the polymer and functional linker. These new matrices proved to be potent solid phases for the synthesis of DNA, RNA, or modified oligonucleotides as well as randomized mixed 2 ′-ribo/2 ′-deoxy-2 ′-fluoro-RNA libraries and/or DNA libraries, randomized with trinucleotides (codons). 相似文献
7.
Adaptability of Silica Gel as a Culture Medium 总被引:2,自引:0,他引:2
Sterges AJ 《Journal of bacteriology》1942,43(3):317-327
8.
K. C. Gupta Pradeep Kumar Dimpan Bhatia A. K. Sharma 《Nucleosides, nucleotides & nucleic acids》2013,32(3-5):829-832
Abstract A rapid method is described for the covalent anchoring of appropriately protected 2′-deoxyribonucleoside-3′-O-succinates to LCAA-CPG, widely used support for solid phase oligonucleotide synthesis. The method involves the reaction of nucleoside-3′-O-succinates with aminoalkyl functions of the support in the presence of improved and commercially available condensing reagent, TBTU or TPP-DTNP to generate fully functionalised polymer supports with excellent nucleoside loadings. 相似文献
9.
用硅胶作载体,戊二醛作交联剂,制备了固定化的纤维素酶。对制备固定化纤维素酶的偶联剂浓度、pH、给酶量3个影响因素进行了研究,通过正交试验优化得出最佳的固定化条件:交联剂戊二醛浓度为1%,固定化pH值为5,固载量为每克载体100mg纤维素酶。 相似文献
10.
DONALD J. REINHARDT 《The Journal of eukaryotic microbiology》1966,13(2):225-226
SYNOPSIS. Modifications of the silica gel technic of Perkins and Ogata have been successfully used to preserve Acrasis rosea for 6–13 months and Dictyostelium purpureum for 31 months. The technic is simple, reliable and inexpensive and is recommended for its possible application for the general preservation of certain fungi and protozoa. 相似文献
11.
Sigmund Gunzenhauser Ewa Biala Peter Strazewski 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1223-1224
Abstract 3,6,9-Trioxaundecane-1,11-diisocyanate (6) was synthesised from tetraethylene glycol in 5 steps and 48 % overall yield. Spacer 6 was monofunctionalised with the fully protected adenosyl-3′-O-succinate derivative 7 and linked to aminomethyl polystyrene affording a solid support suitable for oligoribonucleotide synthesis (loading: ~20 μmol/g). The HPLC analysis of a crude oligoribonucleotide synthesis and the yield of the full-length product show that this spacer compares well to hexamethylene diamine. 相似文献
12.
《Nucleosides, nucleotides & nucleic acids》2013,32(5-8):629-633
Abstract Dimethylthiuram disulfide (DTD) has been developed as an efficient thiolation reagent during automated synthesis of oligonucleotides using phosphoramidite chemistry. Simultaneous thiolation and capping was accomplished by mixing DTD with capping solution B, which saved 20% of solvent consumption and compressed the four-step synthesis cycle to three. Large-scale (1 mmol) synthesis of phosphorothioate oligonucleotides has been demonstrated with excellent yield and purity. 相似文献
13.
Dr. Sherif M. H. Sanad Alshimaa A. M. Abdelsalam Aya A. Gamal Eldin Esraa H. Abdelfattah Fatma R. M. Hussein Nada G. Mohammed Nariman A. S. Taha Prof. Dr. Ahmed E. M. Mekky 《化学与生物多样性》2023,20(6):e202300546
An efficient protocol was adopted to efficiently prepare three new series of bis(pyrazolo[1,5-a]pyrimidines) linked to different spacers. The new bis(pyrazolo[1,5-a]pyrimidines) were prepared in 80–90 % yields by reacting the respective bis(enaminones) and 4-(4-substituted benzyl)-1H-pyrazole-3,5-diamines in pyridine at reflux temperature for 5–7 h. The new products showed a wide spectrum of antibacterial activity against six different bacterial strains. In general, propane- and butane-linked bis(pyrazolo[1,5-a]pyrimidines), which are attached to 3-(4-methyl- or 4-methoxybenzyl) units, had the best antibacterial activity with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values up to 2.5 and 5.1 μM, respectively. Additionally, the previous products demonstrated promising MurB inhibitory activity with IC50 values up to 7.2 μM. 相似文献
14.
Daisuke Kumano Soichiro Iwahana Hiroki Iida Chengshuo Shen Jeanne Crassous Eiji Yashima 《Chirality》2015,27(8):507-517
Acetylated and/or 3,5‐dimethylphenylcarbamated riboflavins were prepared and the resulting riboflavin derivatives as well as natural riboflavin were regioselectively immobilized on silica gel through chemical bonding at the 5’‐O‐ or 3‐N‐position of the riboflavin to develop novel chiral stationary phases (CSPs) for enantioseparation by high‐performance liquid chromatography (HPLC). The chiral recognition abilities of the obtained CSPs were significantly dependent on the structures of the riboflavin derivatives, the position of the chemical bonding on the silica gel, and the structures of the racemic compounds. The CSPs bonded at the 5’‐O‐position on the silica gel tended to well separate helicene derivatives, while the CSPs bonded at the 3‐N‐position composed of acetylated and 3,5‐dimethylphenylcarbamated riboflavins showed a better resolving ability toward helicene derivatives and bulky aromatic racemic alcohols, respectively, and some of them were completely separated into the enantiomers. The observed difference in the chiral recognition abilities of these riboflavin‐based CSPs is discussed based on the difference in their structures, including the substituents of riboflavin and the positions immobilized on the silica gel. Chirality 27:507–517, 2015. © 2015 Wiley Periodicals, Inc. 相似文献
15.
Abstract Several oligodeoxynucleotides had been synthesized on controlled pore glass beads using pure and stable 3′-O(5′-O, N-protected) nucleoside-methyl-N-morpholino phosphoramidites. The average coupling yield at each condensation step was about 94%. 相似文献
16.
A simplified and reliable method for the preparation of a variety of silica gel media is described. The growth of common microorganisms on these media was examined. 相似文献
17.
Marine and nonmarine species of bacteria grew well when a simplified silica gel plate preparation was used. 相似文献
18.
19.
T. Abramova T. Ivanova V. Koval O. Fedorova 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1515-1516
Abstract The derivatives of oligonucleotides with carboxysubstituted porphyrins and phthalocyanins, Fe(III)hematoporphyrin, Pd(II)coproporphyrin I and Co(II)tetracarboxyphthalocyanin, were synthesized using the common approach. 相似文献
20.
P. Kumar A. K. Sharma P. Sharma B. S. Garg K. C. Gupta 《Nucleosides, nucleotides & nucleic acids》2013,32(4):879-888
Abstract A rapid and general one-pot method is described for the attachment of the leader nucleoside onto the polymer supports, suitable for polymer supported oligonucleotide synthesis following oxidation-reduction condensation. The method can also be used for support functionalisation in fully automated DNA synthesizer prior to oligonucleotide synthesis. 相似文献