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Transcriptome sequencing reveals abundant olfactory genes in the antennae of the rice leaffolder,Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) 下载免费PDF全文
Su Liu Wen‐Long Wang Yu‐Xing Zhang Bang‐Xian Zhang Xiang‐Jun Rao Xiao‐Ming Liu Dong‐Ming Wang Shi‐Guang Li 《Entomological Science》2017,20(1):177-188
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Yosuke Fukutani Ayaka Hori Satoshi Tsukada Ryoichi Sato Jun Ishii Akihiko Kondo Hiroaki Matsunami Masafumi Yohda 《Analytical biochemistry》2015
Olfaction depends on the selectivity and sensitivity of olfactory receptors. Previous attempts at constructing a mammalian olfactory receptor-based artificial odorant sensing system in the budding yeast Saccharomyces cerevisiae suffered from low sensitivity and activity. This result may be at least in part due to poor functional expression of olfactory receptors and/or limited solubility of some odorants in the medium. In this study, we examined the effects of two types of accessory proteins, receptor transporting protein 1 short and odorant binding proteins, in improving odor-mediated activation of olfactory receptors expressed in yeast. We found that receptor transporting protein 1 short enhanced the membrane expression and ligand-induced responses of some olfactory receptors. Coexpression of odorant binding proteins of the silkworm moth Bombyx mori enhanced the sensitivity of a mouse olfactory receptor. Our results suggest that different classes of accessory proteins can confer sensitive and robust responses of olfactory receptors expressed in yeast. Inclusion of accessory proteins may be essential in the future development of practical olfactory receptor-based odorant sensors. 相似文献
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The role of the coreceptor Orco in insect olfactory transduction 总被引:1,自引:0,他引:1
Monika Stengl Nico W. Funk 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2013,199(11):897-909
Insects sense odorants with specialized odorant receptors (ORs). Each antennal olfactory receptor neuron expresses one OR with an odorant binding site together with a conserved coreceptor called Orco which does not bind odorants. Orco is necessary for localization of ORs to dendritic membranes and, thus, is essential for odorant detection. It forms a spontaneously opening cation channel, activated via phosphorylation by protein kinase C. Thereafter, Orco is also activated via cyclic adenosine monophosphate (cAMP). Orco forms homo—as well as heteromers with ORs with unknown stoichiometry. Contradictory publications suggest different mechanisms of olfactory transduction. On the one hand, evidence accumulates for the employment of more than one G protein-coupled olfactory transduction cascade in different insects. On the other hand, results from other studies suggest that the OR–Orco complex functions as an odorant-gated cation channel mediating ionotropic signal transduction. This review analyzes conflicting hypotheses concerning the role of Orco in insect olfactory transduction. In conclusion, in situ studies in hawkmoths falsify the hypothesis that Orco underlies odorant-induced ionotropic signal transduction in all insect species. Instead, Orco forms a metabotropically gated, slow cation channel which controls odorant response threshold and kinetics of the sensory neuron. 相似文献