The chemistry of insect hemolymph. II. Trehalose and other carbohydrates

α,α-Trehalose, a sugar previously regarded as a product characteristic of certain lower plants, has been identified as a major blood sugar of insects. Trehalose has been isolated in pure form from the blood of pupae of the silk moth, Telea polyphemus, and has been recognized chromatographically in all the insects examined, which comprise 10 species belonging to 5 different orders. Trehalose has been determined quantitatively with anthrone after either chromatographic separation or chemical degradation of other sugars. In larvae and pupae of 4 species of Lepidoptera it ranges from 0.2 to 1.5 gm. per 100 ml. of blood and makes up over 90 per cent of the blood sugar; in larvae of a sawfly, about 80 per cent of the blood sugar is trehalose. In Bombyx mori and Platysamia cecropia, the pupal blood trehalose level is about half that in the mature larva, suggesting utilization of trehalose for glycogen synthesis during pupation. Small amounts of glucose and apparent glycogen are also present in the plasma of these insects. In Bombyx larval plasma there is also 0.04 to 0.12 gm. per 100 ml. of glucose-6-phosphate and smaller amounts of an apparent ketose phosphate.     

Etiology of breast cancer. II. Epidemiologic aspects

The epidemiology of breast cancer is reviewed with particular emphasis on its etiology. A number of studies suggest that differences in breast cancer incidence are associated with differences in marital status, number of pregnancies, age at menarche, age at menopause, height and weight, socioeconomic status, geographic location and residence. However, in no case is the evidence adequate to establish a “cause and effect” relationship. The genetic component of these associations may be of primary importance, while other conditions such as marital status are probably indirect reflections of the operation of more fundamental factors.There is a general consensus that endocrine factors play an important part in mammary cancer occurrence. At present, the association between breast cancer and the presence of the virus-like (type B) particles in human milk is not established.     

Fine needle aspiration cytology of lobular breast carcinoma. Comparison with other breast lesions

OBJECTIVE: To analyze the detailed cytomorphology of lobular breast carcinoma and to compare the cytologic smears of benign, borderline and infiltrating duct carcinoma. STUDY DESIGN: Fine needle aspiration cytology (FNAC) smears of histopathologically proven infiltrating lobular carcinoma (ILC), infiltrating ductal carcinoma (IDC), borderline lesions and benign breast lesions were selected for study. Detailed cytomorphologic analysis of the smears was carried out on hematoxylin and eosin- and May-Grünwald-Giemsa--stained slides, and a comparison of results was done. The various cytologic features were also graded semi-quantitatively with the numerical score; logistic regression analysis was done. RESULTS: There were 25 cases of ILC, 30 of IDC, and 10 borderline and 18 benign lesions. Cytologic diagnosis of malignancy on FNAC smears of ILC was offered in 19 cases; of them, 2 cases were diagnosed as ILC. The remaining six cases were diagnosed as borderline (four) and benign lesions (two). Overall sensitivity in detection of malignancy in ILC cases was 76%. FNAC smears of ILC showed moderate (52%) to abundant (32%) cellularity. The cells of ILC were arranged both in clusters and in dissociation (72%). Individual cells were monomorphic (40%) to mildly pleomorphic (60%), and the cells were smaller. The cells showed a smooth, regular nuclear margin; bland chromatin; and indistinct nucleoli. Indian file arrangement was frequently observed (28%). Nuclear molding (28%) and intranuclear inclusions (16%) were also noted. Intracytoplasmic lumina were seen in occasional cases. Logistic regression analysis was carried out, and a comparison between lobular carcinoma and ductal carcinoma, and borderline and benign lesions was done. Logistic regression analysis of cytomorphologic features showed that cellularity and nuclear margin irregularities were the two most important features to distinguish ILC from IDC. In comparison to borderline lesions, cell dissociation was more common in ILC. The salient cytologic features that helped to distinguish ILC from benign lesions was cell size, cellularity, cell uniformity and chromatin pattern. CONCLUSION: There are overlapping cytologic features between ILC, IDC and borderline breast lesions on FNAC smears. Logistic regression analysis may be helpful in this regard.     

Carcinoma in a breast fibroadenoma

A 48-year-old woman presented with a long history of a lump in the breast, which was clinically diagnosed as a fibroadenoma. Cytologic examination of fluid aspirated intraoperatively demonstrated groups of carcinoma cells. Excision of the mass showed that this discrepancy was due to carcinoma within a fibro adenoma. Reports of such an occurrence are few, and none, to the best of our knowledge, has previously documented the cytologic findings.     

The Saline Lakes of Saskatchewan II. Locale,Hydrography and other Physical Aspects

The geology and physiography, climate, soils and vegetation of the saline lakes region of southern Saskatchewan are briefly reviewed. The location and morphometry of 60 saline lakes was determined. Some of them are large (307 km²) but mean depths do not exceed 10 m and many are very shallow. Light penetration varied considerably from very turbid shallow lakes to Redberry Lake where the 1% light level was below 16.5 m. Light extinction coefficients for the entire water column of deeper lakes (7) varied from 0.256 to 2.558. Shallow lakes failed to stratify thermally but a dozen deeper lakes stratified for at least several months. Higher salinity tends to prolong stratification. Thermal stratification augmented chemical stratification in two meromictic lakes. Maximum temperature up to 30 °C in very saline shallow waters was recorded while temperatures as low as-3 °C occurred under the ice. Freezing was delayed and thawing accelerated in more saline lakes. Annual heat budgets varied from 3350 to 13,900 g cal cm-² in seven lakes. Oxygen concentration was reduced below thermoclines and was virtually absent below the ice in very shallow lakes and, at times, in a very productive lake.     

Nimodipine's interactions with other drugs: II. Diazepam

Adult Binghamton Heterogeneous (HET) stock mice were administered one of three doses of diazepam (0.1, 2.5, or 5.0 mg/kg) immediately followed by a second injection of either the slow calcium channel blocker, nimodipine (Bay e 9736), or its vehicle. Hypothermic responses and muscular incoordination were measured twenty and sixty minutes later as assessed by changes in rectal temperature and motoric activity on a rotating rod. Nimodipine (5 mg/kg) alone did not significantly affect body temperature or motor coordination. However, when administered in combination with the two highest doses of diazepam, nimodipine significantly potentiated the hypothermic response produced by these doses both twenty minutes and sixty minutes post-injection. Administration of high doses of diazepam (2.5 and 5.0 mg/kg) resulted in significant motor incoordination at both observation periods, but this effect was not potentiated by nimodipine.     

Carcinoma of the breast occurring in routine reduction mammaplasty

We report the case of an 18-year-old female undergoing routine reduction mammaplasty in whom bilateral diffuse intraductal cribriform breast carcinoma was reported upon review of the surgical specimen. This highlights the importance not only of sending specimens for pathologic examination, but also of marking the specimens accurately by location, such as medial, central, and lateral. Treatment with bilateral modified radical mastectomy with lower axillary dissection and immediate reconstruction with autogenous tissue is reported.