Cohesin-Dependent Association of Scc2/4 with the Centromere Initiates Pericentromeric Cohesion Establishment

1. Download : Download high-res image (150KB)
2. Download : Download full-size image

Highlights? Cohesin is required for the association of its loader, Scc2/4, with centromeres ? The Ctf19 kinetochore subcomplex targets the loader to centromeres ? Centromere-loaded cohesin ensures pericentromeric cohesion establishment ? Cohesin ring formation triggers cohesin loading via Scc2/4     

The prereplication complex recruits XEco2 to chromatin to promote cohesin acetylation in Xenopus egg extracts

1. Download : Download high-res image (192KB)
2. Download : Download full-size image

Highlights? XEco2 loading and cohesin acetylation require pre-RC assembly but not DNA synthesis ? Two N-terminal motifs in XEco2 are essential for XEco2 loading and cohesion ? The PIP box in XEco2 contributes to cohesion ? Interaction of acetylated cohesin with DNA is stabilized after DNA replication     

The RNA Pol II Elongation Factor Ell3 Marks Enhancers in ES Cells and Primes Future Gene Activation

1. Download : Download high-res image (254KB)
2. Download : Download full-size image

Highlights? Ell3 is preferentially found at active, poised, and inactive enhancers in ES cells ? Promoter-proximal Pol II occupancy at key developmental genes requires Ell3 ? Cohesin functions in Ell3-associated enhancer-promoter communication ? Recruitment of P-TEFb within SEC during differentiation is Ell3 dependent     

An Auxin Transport Mechanism Restricts Positive Orthogravitropism in Lateral Roots

1. Download : Download high-res image (342KB)
2. Download : Download full-size image

Highlights? Emerging lateral roots show conserved and partially suppressed orthogravitropism. ? Low and high auxin levels lead to radial and axial root systems, respectively. ? Auxin-dependent regulation of GSA requires PIN auxin efflux carrier activity. ? Suppression of PIN4/PIN7 and temporal PIN3 expression limit earliest LR GSA formation     

Short Telomeres in ESCs Lead to Unstable Differentiation

1. Download : Download high-res image (199KB)
2. Download : Download full-size image

Highlights? ESCs with short telomeres do not stably differentiate and can resume proliferation ? Critically short telomeres impair Nanog regulation during ESC differentiation ? Short telomeres elicit genome-wide DNA hypomethylation and altered H3K27me3 levels ? Impaired DNA methylation and differentiation are rescued by Dnmt3b or telomere elongation     

Direct and Indirect Gene Regulation by a Life-Extending FOXO Protein in C. elegans: Roles for GATA Factors and Lipid Gene Regulators

1. Download : Download high-res image (290KB)
2. Download : Download full-size image

Highlights? DAF-16 regulates genes both cell autonomously and cell nonautonomously ? DAF-16 can act at in the endoderm to delay muscle aging ? DAF-16 can act at a distance to protect animals from amyloid paralysis ? Cell-non-autonomous gene regulation by DAF-16 involves the lipid-gene regulator MDT-15     

A comprehensive genomic binding map of gene and chromatin regulatory proteins in Saccharomyces

1. Download : Download high-res image (328KB)
2. Download : Download full-size image

Highlights? Genomic maps of 200 yeast regulators in normal and stress conditions provide a compendium for discovery ? SAGA assembly pathway is associated with more regulation than the TFIID pathway ? SAGA-linked regulators are distal, and TFIID-linked regulators are proximal to the promoter ? During heat shock, SAGA- and TFIID-linked regulators displayed distinct mobilization patterns     

Variation in the Dorsal Gradient Distribution Is a Source for Modified Scaling of Germ Layers in Drosophila

1. Download : Download high-res image (196KB)
2. Download : Download full-size image

Highlights? The germ layers of Drosophila species are unequally scaled to size during evolution ? Evolutionary changes in nuclear size and density affect the Dl gradient scaling ? Mesodermal variations rely on Dl distribution instead of regulation of target genes ? Dl gradient distortions allow new positioning of ventral neuroectodermal border     

Differential Regulation of Protrusion and Polarity by PI(3)K during Neutrophil Motility in Live Zebrafish

1. Download : Download high-res image (220KB)
2. Download : Download full-size image

Highlights? Neutrophil motility in live zebrafish requires PI(3)K activity at the leading edge ? Localized photo-activation of Rac can to direct migration in vivo ? Rac activation rescues protrusion but not polarization in PI(3)K-inhibited cells ? Separable two-tier regulation of F-actin dynamics and protrusion by PI(3)K     

HnRNP L and HnRNP A1 Induce Extended U1 snRNA Interactions with an Exon to Repress Spliceosome Assembly

1. Download : Download high-res image (130KB)
2. Download : Download full-size image

Highlights? HnRNP L recruits hnRNP A1 and U1 snRNA to sequences upstream of the 5′ splice site ? HnRNP L-induced extended base pairing of U1 snRNA inhibits exchange for U6 snRNA ? Local traps in spliceosome assembly are naturally exploited mechanisms of regulation ? Extended base pairing of U1 may account for much of regulation by hnRNPs L and A1     

Insights into Negative Regulation by the Glucocorticoid Receptor from Genome-wide Profiling of Inflammatory Cistromes

1. Download : Download high-res image (136KB)
2. Download : Download full-size image

Highlights? TLR4 activation dramatically remodels the GR cistrome in macrophages ? Both negative and positive GR enhancers include GREs, NF-κB, and AP-1 sites ? Classical models fail to predict positive or negative polarity of GR regulation ? Hormone treatment marks negative enhancers by histone deacetylation     

Calorie Restriction and SIRT3 Trigger Global Reprogramming of the Mitochondrial Protein Acetylome

1. Download : Download high-res image (421KB)
2. Download : Download full-size image

Highlights? MS quantifies 1,578 mitochondrial acetyl sites altered during CR and loss of SIRT3 ? SIRT3 functions as a prominent regulator in CR adaptation ? CR and SIRT3 regulate previously unrecognized processes in mitochondria ? We provide an acetylation atlas for understanding mitochondrial regulation in CR     

ASH2L Regulates Ubiquitylation Signaling to MLL: trans-Regulation of H3 K4 Methylation in Higher Eukaryotes

1. Download : Download high-res image (146KB)
2. Download : Download full-size image

Highlights? ASH2L WH motif is important for trans-regulation of H3 K4 methylation by H2Bub ? Ub stimulates MLL activity in trans, in contrast to DOT1L regulation by H2Bub ? H2Bub enhances MLL, but not MLL3, methyltransferase activity ? H2Aub directly represses MLL methyltransferase activity     

SIP1 Mediates Cell-Fate Decisions between Neuroectoderm and Mesendoderm in Human Pluripotent Stem Cells

1. Download : Download high-res image (184KB)
2. Download : Download full-size image

Highlights? Smad-interacting protein 1 (SIP1) regulates hESC differentiation ? SIP1 upregulation promotes neuroectodermal differentiation ? SIP1 inhibits mesendodermal and endodermal differentiation ? SMAD2/3 and NANOG/OCT4/SOX2 cooperatively regulate SIP1 expression     

Keeping cell identity in Arabidopsis requires PRC1 RING-finger homologs that catalyze H2A monoubiquitination

1. Download : Download high-res image (139KB)
2. Download : Download full-size image

Highlights? AtBMI1A/B proteins prevent misexpression of embryonic traits in somatic cells ? PRC2 and AtBMI1A/B proteins maintain cells in their differentiated state ? AtBMI1A/B proteins mediate H2A monoubiquitination     

Perivascular Nitric Oxide Activates Notch Signaling and Promotes Stem-like Character in PDGF-Induced Glioma Cells

1. Download : Download high-res image (229KB)
2. Download : Download full-size image

Highlights? Perivascular niche nitric oxide activates Notch in PDGF-induced gliomas ? Notch signaling drives tumor stem-like characteristics and reduces survival ? Loss of eNOS activity decreases tumor stem-like characteristics ? Suppressing eNOS activity prolongs survival in glioma-bearing mice     

System-wide Rewiring Underlies Behavioral Differences in Predatory and Bacterial-Feeding Nematodes

1. Download : Download high-res image (343KB)
2. Download : Download full-size image

Highlights? Massive neural circuit rewiring between nematodes with divergent feeding behavior ? Striking patterns correlate with known functional differences ? Motor synapse differences indicate a substantial change in information flow ? Correlates of predatory feeding behavior are identified     

Evidence for Geomagnetic Imprinting as a Homing Mechanism in Pacific Salmon

1. Download : Download high-res image (378KB)
2. Download : Download full-size image

Highlights? Sockeye salmon use geomagnetic imprinting as a homing mechanism ? The homing route of salmon is predicted by magnetic field drift (secular variation)