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1.
M. Asgher S. A. H. Shah M. Ali R. L. Legge 《World journal of microbiology & biotechnology》2006,22(1):89-93
Summary Four white-rot fungi isolated in Pakistan were used for decolorization of widely used reactive textile dyestuffs. Phanerochaete chrysosporium, Coriolus versicolor, Ganoderma lucidum and Pleurotus ostreatus were grown in defined nutrient media for decolorization of Drimarene Orange K-GL, Remazol Brilliant Yellow 3GL, Procion BluePX-5R
and Cibacron Blue P-3RGR for 10 days in shake flasks. Samples were removed every day, centrifuged and the absorbances of the
supernatants were read to determine percentage decolorization. It was observed that P. chrysosporium and C. versicolor could effectively decolorize Remazol Brilliant Yellow 3GL, Procion BluePX-5R and Cibacron Blue P-3RGR. Drimarene Orange K-GL
was completely decolorized (0.2 g/l after 8 days) only by P.chrysosporium, followed by P. ostreatus (0.17 g/l after 10 days). P. ostreatus also showed good decolorization efficiencies (0.19–0.2 g/l) on all dyes except Remazol Brilliant Yellow (0.07 g/l after 10 days).
G. lucidum did not decolorize any of the dyestuffs to an appreciable extent except Remazol Brilliant Yellow (0.2 g/l after 8 days). 相似文献
2.
Effect of various parameters such as size of inoculum, temperature, carbon source on decolorization of textile wastewater by Phanerochaete chrysosporium was investigated. Textile wastewater decolorization occurred during the primary phase of growth and secondary metabolism in carbon and nitrogen limited medium, respectively. It was found that glucose concentration up to 0.3 g/l has considerable effect on decolorisation rate. Further, it was also found that the concentration of the organic nitrogen of the effluent stream was sufficient to furnish the decolorisation process. It was observed that the inoculum size in this case within 10% increased the decolorisation rate rapidly. It was found that the temperature rise from 20 to 38 °C enhanced the rate of decolorization. The optimum temperature for decolorisation was found to be about 35 °C. Effect of pH from 2-4 on decolorization was also investigated. It is concluded that using Phanerochaete chrysosporium, decolorization of the azo dye containing effluent of the textile industry was achieved to about 96% within 28 h of operation. 相似文献
3.
Moredo N. Lorenzo M. Domínguez A. Moldes D. Cameselle C. Sanroman A. 《World journal of microbiology & biotechnology》2003,19(7):665-669
Ligninolytic enzyme production by the white-rot fungi Phanerochaete chrysosporium and Trametes versicolor precultivated with different insoluble lignocellulosic materials (grape seeds, barley bran and wood shavings) was investigated. Cultures of Phanerochaete chrysosporium precultivated with grape seeds and barley bran showed maximum lignin peroxidase (LiP) and manganese-dependent peroxidase (MnP) activities (1000 and 1232 U/l, respectively). Trametes versicolor precultivated with the same lignocellulosic residues showed the maximum laccase activity (around 250 U/l). For both fungi, the ligninolytic activities were about two-fold higher than those attained in the control cultures. In vitro decolorization of the polymeric dye Poly R-478 by the extracellular liquid obtained in the above-mentioned cultures was monitored in order to determine the respective capabilities of laccase, LiP and MnP. It is noteworthy that the degrading capability of LiP when P. chrysosporium was precultivated with barley bran gave a percentage of Poly R-478 decolorization of about 80% in 100 s, whereas control cultures showed a lower percentage, around 20%, after 2 min of the decolorization reaction. 相似文献
4.
Ola M. Gomaa John E. Linz C. A. Reddy 《World journal of microbiology & biotechnology》2008,24(10):2349-2356
Synthetic textile dyes are among the most dangerous chemical pollutants released in industrial wastewater streams. Recognizing
the importance of reducing the environmental impact of these dyes, the ability of the white rot fungus Phanerochaete chrysosporium to decolorize various textile dyes was investigated. This fungus decolorized 6 of the 14 structurally diverse dyes with varying
efficiency (between 14% and 52%). There was no discernable pattern of decolorization even among dyes of the same chemical
class, suggesting that attack on the dyes is relatively non-specific. Among the three dyes which showed >40% decolorization,
Victoria Blue B (VB) was chosen for further analysis because the ability of the fungus to decolorize VB was nearly independent
over a relatively broad concentration range. Blocking lignin peroxidase (LiP) and manganese peroxidase (MnP) production by
the fungus did not substantially affect VB decolorization. Inhibition of laccase production by adding various inhibitors to
shaken cultures reduced VB decolorization significantly suggesting a role for laccase in VB decolorization. When sodium azide
and aminotriazole were used to inhibit endogenous catalase and cytochrome P-450 oxygenase activities, there was 100% and 70%
reduction in VB decolorization, respectively. Adding benzoate to trap hydrogen peroxide-derived hydroxyl radicals resulted
in 50% decolorization of VB. Boiling the extracellular fluid (ECF) for 30 min resulted in approximately 50% reduction in VB
decolorization. Collectively, these data suggest that laccase, and/or oxygenase/oxidase and a heat-stable non-enzymatic factor,
but not Lip and MnP, play a role in VB decolorization by P. chrysosporium. 相似文献
5.
The evaluation of pre-grown mycelial pellets in decolorization of textile dyes during repeated batch process 总被引:1,自引:0,他引:1
Ozfer Yesilada Seval Cing Yildirim Emre Birhanli Elif Apohan Dilek Asma Filiz Kuru 《World journal of microbiology & biotechnology》2010,26(1):33-39
This study was undertaken for the possibility of application of pre-grown pellets for biotechnological treatment of dyes and
textile industry waste waters. Mycelial pellets of five different white rot fungi were tested for their dye decolorization
activity. The pellets of Funalia trogii, Phanerochaete chrysosporium and Trametes versicolor were determined as the most effective ones. The decolorization ability of viable pellets was compared with the decolorization
(adsorption) ability of dead pellets during repeated batch studies. Astrazon Black dye was decolorized effectively, about
90%, by viable pellets of all fungi during the first use. Viable F. trogii pellets were found as the most effective pellets. Upon pellet treatment not only a high decolorization but also reduced toxicity
(antimicrobial activity) of the Astrazon Black dye was recorded. This type of decolorization activity with commercial or crude
laccase was partially observed. Growing cells of F. trogii in batch system showed lower efficiency in color removal of mixed dyes compared to the pre-grown pellets in repeated batch
system. The results in this study showed that mycelial pellets could effectively be used as an alternative to traditional
physicochemical processes. 相似文献
6.
Roland Waldner Matti S. A. Leisola Armin Fiechter 《Applied microbiology and biotechnology》1988,29(4):400-407
Summary Six fast growing ligninolytic white-rot fungi were compared with Phanerochaete chrysosporium. The results showed that the fungi have similar ligninolytic systems, although minor differences exist. Like in P. chrysosporium the ligninolytic system could be induced by veratryl alcohol in Coriolus versicolor and Chrysosporium pruinosum. These three lignin peroxidase producing fungi were the fastest lignin degraders in stationary cultures, whereas in agitated cultures Bjerkandera adusta showed highest lignin degradation rates. Metabolites accumulating during the degradation of veratryl alcohol were analyzed and compared. Peroxidase production seems to be a common feature of all the tested fungi. Polyclonal antibodies against the lignin peroxidase with pl of 4.65 from P. chrysosporium reacted with the extracellular peroxidases of C. pruinosum, C. versicolor and B. adusta, but not with those of Pleurotus ostreatus.Dedicated to Professor Dr. Hans-Jürgen Rehm on the occasion of his 60th birthday 相似文献
7.
The white-rot fungi Trametes versicolor PRL 572, Trametes versicolor MUCL 28407, Pleurotus ostreatus MUCL 29527, Pleurotus sajor-caju MUCL 29757 and Phanerochaete chrysosporium DSM 1556 were investigated for their ability to degrade the polycyclic aromatic hydrocarbons (PAH) anthracene, benz[a]anthracene and dibenz[a,h]anthracene in soil. The fungi were grown on wheat straw and mixed with artificially contaminated soil. The results of this
study show that, in a heterogeneous soil environment, the fungi have different abilities to degrade PAH, with Trametes showing little or no accumulation of dead-end metabolites and Phanerochaete and Pleurotus showing almost complete conversion of anthracene to 9,10-anthracenedione. In contrast to earlier studies, Phanerochaete showed the ability to degrade the accumulated 9,10-anthracenedione while Pleurotus did not. This proves that, in a heterogeneous soil system, the PAH degradation pattern for white-rot fungi can be quite different
from that in a controlled liquid system.
Received: 20 March 1996 / Received revision: 2 July 1996 / Accepted: 8 July 1996 相似文献
8.
The decontamination of effluents from textile industries is problematic due to the fact that textile dyes are resistant to degradation in the environment. Enzymes from white rot fungi, especially laccase, are able to degrade various complex aromatic structures, and are therefore able to decolorize textile dyes. The white‐rot fungi Trametes versicolor and Phanerochaete chrysosporium were immobilized, separately, on both pine wood chips and palm oil fiber, and cultivated in the temporary immersion RITA® (Récipient à Immersion Temporaire Automatique) System, which was adapted to serve as a fungal bioreactor in a series of four experiments to determine optimal conditions for decolorizing the textile dyes Levafix Blue and Remazol Brilliant Red. The maximum rate of decolorization of both dyes occurred within 24 h of incubation, and laccase was detected in the system. 相似文献
9.
Tripathi MK Mishra AS Misra AK Vaithiyanathan S Prasad R Jakhmola RC 《Letters in applied microbiology》2008,46(3):364-370
Aims: Selection of white‐rot fungi of bio‐conversion of mustard straw (MS) into feed for ruminants. Methods and Results: Mustard straw was cultured with Ganoderma applanatum, Coriolus versicolor and Phanerochaete chrysosporium for solid‐state fermentation at 35°C from 7 to 63 days for dilignification and for 21 days to study dry matter digestibility and protein enrichment. Lignin loss in fungus cultured straw varied between 100 and 470 g kg?1 lignin. Dilignification was higher between 7 and 28 days fermentation with C. versicolor. Among the three fungi P. chrysosporium was the most effective in degrading lignin for longer fermentation. In‐vitro dry matter digestibility (IVDMD) and crude protein content was higher in C. versicolor cultured straw. Large quantity of straw was cultured by C. versicolor for 21 days, for in vivo evaluation. Mean pH and metabolites of rumen fermentation were not different while, pH and volatile fatty acid increased at 6 h postfermentation on cultured straw feeding. Cultured straw fermentation increased (P = 0·001) small holotricks and reduced (P = 0·005) large holotricks population. Fungus cultures straw did not improve microbial enzyme concentration. Conclusions: Coriolus versicolor and P. chrysosporium were the promising fungus for MS bio‐dilignification. Significance and Impact of the Study: Coriolus versicolor treated MS improved dry matter digestibility and protein content. 相似文献
10.
Textile industry discharges a vast amount of unused synthetic dyes in effluents. The discharge of these effluents into rivers and lakes leads to a reduction in sunlight penetration in natural water bodies, which, in turn, decreases both photosynthetic activity and dissolved oxygen concentration rendering it toxic to living beings. This paper describes the decolorization potential of a local white rot fungus, Coriolus versicolor IBL-04 for practical industrial effluents collected from five different textile industries of Faisalabad, Pakistan. Screening of C. versicolor IBL-04 on five effluents showed best decolorization results (36.3%) for Arzoo Textile Industry (ART) effluent in 6 days followed by Crescent Textile Industry (CRT), Itmad Textile Industry (ITT), Megna Textile Industry (MGT) and Ayesha Textile Industry (AST) effluents. Optimization of different process parameters for ART effluent decolorization by C. versicolor IBL-04 showed that manganese peroxidase (MnP) (486 U/mL) was the lignolytic enzyme present in the culture filtrates with undetectable lignin peroxidase (LiP) and laccase. The MnP synthesis and effluent decolorization could be enhanced to 725 U/mL and 84.4%, respectively, with a significant time reduction to 3 days by optimizing pH and temperature and using 1% starch as a supplementary carbon source. 相似文献
11.
Effect of environmental conditions on biological decolorization of textile dyestuff by C. versicolor
Effects of environmental conditions such as pH, media composition, carbon and nitrogen sources, TOC/N ratio, and dyestuff concentrations on decolorization of reactive phytalocyanin type textile dyestuff Everzol Turquoise Blue G by white rot fungi, Coriolus versicolor 20) or low nitrogen concentration was essential for effective decolorization of the dyestuff. Dyestuff concentration should be lower than 500 mg/l for complete decolorization. Only partial decolorization was observed for dyestuff concentrations above 500 mg/l. Adsorption of the dyestuff on surfaces of the fungi was insignificant (<20%). 相似文献
12.
Degradation and decolorization of monosodium glutamate wastewater (MSGW) with Coriolus versicolor were firstly carried out. The effects of various operation parameters namely wastewater concentrations, pH, culture time
and incidence of sterilization on maximum percentage of degradation and decolorization of wastewater were investigated. Studies
of mycelium and enzyme for C. versicolor degradation and decolorization were estimated in this study. Ten percentage of wastewater concentration and pH = 5.0 were
found to be the most suitable ones among the other experiments. The highest degradation and decolorization efficiency of wastewater
was obtained at the fifth day of cultivation, which was displayed with more than 70% chemical oxygen demand removal, 83% total
sugar removal and 55% color removal, respectively. Sterile operation had no remarkable effect on the degradation and decolorization
efficiency for C. versicolor. Mycelium and the extra cellular fungal enzyme were both necessary for the degradation and decolorization of MSGW. C. versicolor possesses great potential and economic advantages in MSGW treatment. 相似文献
13.
White-rot fungal strains of Trametes versicolor and Phanerochaete chrysosporium were selected to study the decolourisation of the textile dye, Reactive Black 5, under alkaline–saline conditions. Free and immobilised T. versicolor cells showed 100 % decolourisation in the growth medium supplemented with 15 g l?1 NaCl, pH 9.5 at 30 °C in liquid batch culture. Continuous culture experiments were performed in a fixed-bed reactor using free and immobilised T. versicolor cells and allowed 85–100 % dye decolourisation. The immobilisation conditions for the biomass and the additional supply of carbon sources improved the decolourisation performance during a long-term trial of 40 days. Lignin peroxidase, laccase and glyoxal oxidase activities were detected during the experiments. The laccase activity varied depending on carbon source utilized and glycerol-enhanced laccase activity compared to sucrose during extended growth. 相似文献
14.
Ilgi Karapinar Kapdan Fikret Kargi Geoffrey McMullan Roger Marchant 《Biotechnology letters》2000,22(14):1179-1181
A mixed anaerobic bacterial culture decolorized Drimaren Orange K-GL, Everzol Red RBN and Everdirect Supra Yellow PG dyestuffs at 200 mg dyestuff l–1 over 24 h. Improved performance with complete decolorization within 24 h was achieved by incubation with 5 g yeast extract l–1 compared to glucose, lactose and sucrose though 50 mg yeast extract l–1 supplemented with 5 g lactose l–1 or 5 g sucrose l–1 also resulted in complete decolorization within 24 h. 相似文献
15.
Hyeon Woo Kang Yun Hui Yang Sang Woo Kim Soonok Kim Hyeon-Su Ro 《Biotechnology and Bioprocess Engineering》2014,19(3):519-525
Triphenylmethane dyes such as Crystal Violet (CV) and Malachite Green (MG) are common textile dyes. MG, which is toxic to humans, is widely used in aquaculture as an antifungal agent. In this study, 56 mushroom strains from 12 species of wild mushrooms were examined on dye-containing PDA plates to evaluate their potential for the bioremediation of synthetic dyes. Pycnoporus coccineus, Coriolus versicolor, and Lentinula edodes showed fair growth on CV, but only a few survived on MG. However, a decolorization experiment in an aqueous system revealed that the growth on MG-containing solid medium did not directly match the decolorization of MG in the aqueous system. C. versicolor IUM0061 grew well on both MG and CV plates, but could not decolorize MG in the reaction mixture. Conversely, HPLC analysis revealed that P. coccineus IUM0032, which could not grow on the MG plate, completely mineralized MG within 3 days. A subsequent enzyme activity assay revealed a high lignin peroxidase activity in the reaction mixture, indicating that lignin peroxidase is the key enzyme involved in degradation of MG in P. coccineus IUM0032. 相似文献
16.
Zahra Ansari Farbod Ebadi Fard Azar Nour-Ahmad Latifi 《Biocatalysis and Biotransformation》2017,35(6):434-441
An enzymatic reaction using glucose oxidase (GOx) was applied for continues production of hydrogen peroxide and organic acid in Phanerochaete chrysosporium cultures for use simultaneously in catalytic cycle of peroxidases. Decolorization efficiency of crystal violet (CV) as a model pollutant was investigated in 16 d old cultures which overproduced manganese peroxidase (MnP) in response to daily GOx addition and control cultures (i.e. no GOx was added). However, the ability of overproduced cultures in decolorization of CV was not increased significantly, through addition of GOx (300?U/L)?+?glucose (10?Mm) to the culture medium at the start of decolorization, the time needed to obtain 87?±?0.5% removal of CV was reduced 10.7-fold in compared with the control culture. The best GOx concentration in culture medium for more efficient decolorization was obtained to be 300?U/L. These findings indicated that GOx in the presence of glucose could increase the degradation of CV not only by inducing ligninolytic activity in cultures but also as a subsidiary source for in situ H2O2 and organic acid production for catalytic activity of peroxidases in P. chrysosporium cultures. 相似文献
17.
Decolorization of Several Polymeric Dyes by the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium 总被引:28,自引:13,他引:15
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The polymeric dyes Poly B-411, Poly R-481, and Poly Y-606 were examined as possible alternatives to the radiolabeled lignin previously used as a substrate in lignin biodegradation assays. Like lignin degradation, the decolorization of these dyes by the white rot basidiomycete Phanerochaete chrysosporium occurred during secondary metabolism, was suppressed in cultures grown in the presence of high levels of nitrogen, and was strongly dependent on the oxygen concentration in the cultures. A variety of inhibitors of lignin degradation, including thiourea, azide, and 4′-O-methylisoeugenol, also inhibited dye decolorization. A pleiotropic mutant of P. chrysosporium, 104-2, lacking phenol oxidase and ligninolytic activity was also not able to decolorize the polymeric dyes, whereas a phenotypic revertant strain, 424-2, regained this capacity. All of these results suggest that the ligninolytic degradation activity of the fungus was responsible for the decolorization of these dyes. 相似文献
18.
《Process Biochemistry》2007,42(10):1429-1435
In this study, decolorization of Remazol Brillant Blue Royal (RBBR) and Drimaren Blue CL-BR (DB) was investigated using three white rot fungi named as Pleurotus ostreatus (P. ostreatus), Coriolus versicolor (C. versicolor) and Funalia trogii (F. trogii). Decolorization studies were continued for 48 h under static conditions at 30 °C and pH 5.0. The degree of pH, dry mycelium weight (DMW), dye concentration, laccase activity and protein content were analyzed; the enzyme responsible for decolorization was detected for both dyes. Maximum and minimum decolorizations were obtained by F. trogii and P. ostreatus, respectively. Both dyes at all concentrations were found to be toxic for P. ostreatus growth, whereas only DB above 60 mg/L was found to be toxic for C. versicolor growth. Maximum and minimum laccase activities were detected in decolorization media of F. trogii and P. ostreatus, respectively. Results of activity staining following SDS-PAGE showed that laccase is the only enzyme that is responsible for decolorization of DB and RBBR. 相似文献
19.
Cristiane A. Ottoni Cledir Santos Zofia Kozakiewicz Nelson Lima 《Folia microbiologica》2013,58(3):187-193
Twelve white-rot fungal strains belonging to seven different species were screened on plates under alkaline condition to study the decolourisation of the textile dyes Reactive Black 5 and Poly R-478. Three strains of Trametes versicolor (Micoteca da Universidade do Minho (MUM) 94.04, 04.100 and 04.101) and one strain of Phanerochaete chrysosporium (MUM 94.15) showed better decolourisation results. These four strains were used for decolourisation studies in liquid culture medium. All four selected strains presented more efficient decolourisation rates on Reactive Black 5 than on Poly R-478. For both dyes on solid and liquid culture media, the decolourisation capability exhibited by these strains depended on dye concentration and pH values of the media. Finally, the decolourisation of Reactive Black 5 by T. versicolor strains MUM 94.04 and 04.100 reached 100 %. In addition, the highest white-rot fungi ligninolytic enzyme activities were found for these two strains. 相似文献
20.
Chen A Zeng G Chen G Fan J Zou Z Li H Hu X Long F 《Applied microbiology and biotechnology》2011,91(3):811-821
Phanerochaete chrysosporium has been recognised as an effective bioremediation agent due to its unique degradation to xenobiotic and biosorption ability
to heavy metals. However, few studies have focused on the simultaneous removal of heavy metals and organic pollutants. The
aim of this work was to study the feasibility of simultaneous cadmium removal and 2,4-dichlorophenol (2,4-DCP) degradation
in P. chrysosporium liquid cultures. The removal efficiencies were pH dependent and the maximum removal efficiencies were observed at pH 6.5
under an initial cadmium concentration of 5 mg/L and an initial 2,4-DCP concentration of 20 mg/L. The removal efficiencies
for cadmium and 2,4-DCP reached 63.62% and 83.90%, respectively, under the optimum conditions. The high production levels
of lignin peroxidase (7.35 U/mL) and manganese peroxidase (8.30 U/mL) resulted in an increase in 2,4-DCP degradation. The
protein content decreased with increasing cadmium concentration. The surface characteristics and functional groups of the
biomass were studied by scanning electron microscopy and a Fourier-transformed infrared spectrometer. The results showed that
the use of P. chrysosporium is promising for the simultaneous removal of cadmium and 2,4-DCP from liquid media. 相似文献