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细小病毒B19感染实验室诊断技术的研究进展 总被引:1,自引:0,他引:1
人类细小病毒感染,临床可有多种表现,尤其可导致胎儿早产、流产、死胎、贫血、发育迟缓及形成积水型胎儿。本文拟从电镜检查、ELISA、固相放射免疫技术(RIA)、分子杂交及多聚酶链反应(PCR)等几个方面对该病毒感染实验室诊断的国外最新研究进展作一介绍。 相似文献
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无菌留取 5 4例自然流产妇女和 43例妊娠无异常孕妇血清 ,用聚合酶链反应 (PolymeraseChainReaction ,PCR)检测的人细小病毒B19(HumanParvovirusB19,B19)DNA ,在自然流产组中人细小病毒B19DNA有 15例阳性 ,阳性率为 2 7.78%。正常对照组中 ,人细小病毒B19DNA有 2例为阳性 ,阳性率为 4.65 % ,用x2 检验 ,x2 =8.86,P <0 .0 1,两组有非常显著性差异。由此总结 ,人细小病毒B19感染可能是导致自然流产的原因之一 相似文献
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人细小病毒B19基因变异的研究进展 总被引:4,自引:0,他引:4
人细小病毒B19简称B19病毒,是细小病毒中惟一能感染人类的病毒,也是动物病毒中对人类具有致病性的最小单链线状DNA病毒,其与儿童及成人的多种疾病密切相关。B19病毒的基因变异对其致病、传播等具有重要影响,且与B19病毒诊断试剂,疫苗的制备等关系密切。本文就B19病毒基因组的变异状况,基因变异研究方法及研究B19病毒基因变异的意义作一概述。 相似文献
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采用昆虫杆状病毒表达系统,制备人细小病毒B19病毒样颗粒(VLPs)。先通过PCR方法合成细小病毒B19衣壳蛋白基因VP2,将其克隆到pFastBac1质粒,然后转化含杆状病毒穿梭载体Bacmid的E.coliDH10Bac感受态细胞,获得重组杆状病毒表达质粒Bacmid-VP2。在脂质体介导下转染Sf9昆虫细胞,包装重组杆状病毒rBac-VP2。利用rBac-VP2感染Sf9细胞表达B19VP2蛋白,通过间接免疫荧光、Western blotting等方法鉴定目的蛋白表达。采用两次超速离心的方法对表达产物进行纯化,纯化产物在透射电镜下可见直径约22nm的VLPs。本研究成功制备了人细小病毒B19的VLPs,为B19感染血清学检测方法的建立提供了参考。 相似文献
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目的调查人类细小病毒B19在武汉地区普通人群,尤其是育龄妇女中的感染状况。方法采集武汉地区2家医院的血液样本1700份,分为两组。以血清中提取的DNA为模板,进行巢式PCR扩增。结果第Ⅰ组(普通组,包括男性和女性)阳性检测率为4.50%,第Ⅱ组(妇女组)阳性检测率为8.33%。结论武汉地区育龄妇女的B19感染率高于普通人群,很有必要对孕妇进行诊断从而预防新生儿感染B19病毒。另外,由于巢式PCR具有灵敏、特异、简便等优点,适合于用来检测血液样本中的人细小病毒B19。 相似文献
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Liliane Costa Conteville Louise Zanella Michel Abanto Marín Ana Maria Bispo de Filippis Rita Maria Ribeiro Nogueira Ana Carolina Paulo Vicente Marcos César Lima de Mendon?a 《Memórias do Instituto Oswaldo Cruz》2015,110(6):820-821
Parvovirus B19 (B19V) infects individuals worldwide and is associated with an ample
range of pathologies and clinical manifestations. B19V is classified into three
distinct genotypes, all identified in Brazil. Here, we report a complete sequence of
a B19V genotype 1A that was obtained by high-throughput metagenomic sequencing. This
genome provides information that will contribute to the studies on B19V epidemiology
and evolution. 相似文献
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人类细小病毒B19壳蛋白VP2在昆虫杆状病毒表达系统中的表达 总被引:1,自引:1,他引:1
利用BAC-TO-BAC系统获得了人类细小病毒B19壳蛋白VP2的重组昆虫杆状病毒,并在sf9细胞中表达出VP2。用蚀斑法纯化病毒,终末稀释法测定病毒滴度为3.6×108。Western印迹检测证实了表达蛋白的特异性,间接免疫荧光法可观察到细胞胞浆中的表达蛋白颗粒。 相似文献
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Renata Freire Alves Pereira Rita de Cássia Nasser Cubel Garcia Kátia Martins Lopes de Azevedo Sérgio Setúbal Marilda Agudo Mendon?a Teixeira de Siqueira Solange Artimos de Oliveira 《Memórias do Instituto Oswaldo Cruz》2014,109(2):168-173
Immunocompromised patients may develop severe chronic anaemia when infected by human
parvovirus B19 (B19V). However, this is not the case in human immunodeficiency virus
(HIV)-infected patients with good adherence to highly active antiretroviral treatment
(HAART). In this study, we investigated the clinical evolution of five HIV-infected
patients receiving HAART who had B19V infections confirmed by serum polymerase chain
reaction. Four of the patients were infected with genotype 1a strains and the
remaining patient was infected with a genotype 3b strain. Anaemia was detected in
three of the patients, but all patients recovered without requiring immunoglobulin
and/or blood transfusions. In all cases, the attending physicians did not suspect the
B19V infections. There was no apparent relationship between the infecting genotype
and the clinical course. In the HAART era, B19V infections in HIV-positive patients
may be limited, subtle or unapparent. 相似文献
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11kDa蛋白作为B19病毒的一个非结构蛋白,可能在病毒复制周期中发挥重要作用.为了研究11 kDa蛋白对细胞内NF-κB信号通路的影响,首先通过原核表达纯化获得GST-11kDa融合蛋白,并制备免疫血清,利用免疫血清验证了11 kDa蛋白在Hela细胞呈胞浆定位.荧光素酶检测系统发现11 kDa蛋白能上调细胞内NF-κB转录活性,Western blotting进一步表明11 kDa蛋白能够引起细胞内IκB-α的降解.同时,11 kDa蛋白还能够上调细胞内炎性因子IL6启动子的活性,而该反应主要依赖于NF-κB通路.结果表明,11 kDa蛋白通过参与细胞内信号途径激活相关炎性因子的表达. 相似文献
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Parvovirus B19 (PVB19) is the causative agent of erythema infectiosum and sometimes the infection is correlated with severe haematological complications, or in pregnancy to fetalis hydrops. Moreover some authors suggest an infection involvement in some autoimmune diseases. To this purpose we evaluated seroprevalence for PVB19 in following the autoimmune or dysreactive pathologies: systemic lupus erythematosus (SLE), cryoglobulinemia, idiopathic systemic--ANCA associated vasculitis, rheumatoid arthritis (RA). In the case of LES, 31/42 patients were positive for PVB19 versus 21/42 of blood donors, as controls subjects (73.8% vs. 50%; significant difference for p < 0.05), moreover a significant difference for p < 0.001 was detected comparing mean titre values of IgGs against PVB19 of two groups (UI 1.94 +/- 0.90 vs. 1.24 +/- 0.80). In contrast no significant differences were found in the case of percent seropositivity of cryoglobulinemic subjects (37/57 = 64.9%, the majority of whom were HCV+) in comparison with the control group (50%). However mean units index (UI) was 1.63 +/- 0.81; p = 0.019 versus the control group. Similar result, with regard to the percentage of seropositivity, was found for vasculitis (9/17 = 52.9%). The data reported here can confirm a possible correlation between PVB19 prior infection and LES and also suggest possible implications in the case of cryoglobulinemia. In fact, most of our patients were affected by a nephropathic or systemic form of HCV+ cryoglobulinemia and the presence of other infective cofactors could be suggestive in the evolution of this clinical situation. 相似文献
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用PCR检测临床血标本中人类细小病毒B19 DNA 总被引:2,自引:0,他引:2
用PCR检测临床血标本中人类细小病毒B19 DNA杨洪江,张桦,林书祥,牛艾茹(天津市儿童医院儿研所,天津300074)关键词聚合酶链反应(PCR),人类细小病毒B19几种人类细小病毒中,只有B19病毒是人类的病原体。由于该病毒不能在体外繁殖,获得该... 相似文献