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1.
Rectifier-like Activities of Roots of Two Desert Succulents 总被引:13,自引:0,他引:13
Axial and radial water flows for roots in response to appliedhydrostatic pressure drops, water loss from roots after variousperiods of drying, and development of new roots after rewettingdroughted plants were examined for two sympatric desert succulents.Agave deserti Engelm. and Ferocactus acanthodes (Lemaire) Brittonand Rose. For a 40 kPa hydrostatic pressure drop applied to20 mm long root pieces, radial water flows from the epidermisto the root xylem were 2- to 5-fold greater at the tip thanat midlength and were much less than axial flows along the xylem.Upon drying detached roots in air at 20 °C and a water vapoursaturation deficit of 1.2 kPa (50% relative humidity), radialwater flow decreased more than 10-fold in 36 h, and couldrecover to the original level 6 h after rewetting. The rateof water loss from attached roots of plants dried in air at20 °C and a 1.2 kPa saturation deficit decreased about 200-foldin 72 h, which would greatly limit water loss from the plantto a drying soil. At 96 h after rewetting roots of A. desertithat had been exposed to air at 20 °C and a 1.2 kPa saturationdeficit for 120 h, rehydration of existing roots and developmentof new roots contributed about equally to water uptake by thewhole plant. In summary, roots of these desert succulents canreadily take up water from a wet soil but do not lose much waterto a dry soil, thus effectively acting like rectifiers withrespect to plant-soil water movement. Key words: Agave, Cactus, Drought, Root, Water flow, Xylem 相似文献
2.
Localization of ATPase Activity on the Plasmalemma of Scutellar Epithelial Cells of Germinating Barley (Hordeum vulgare L.) 总被引:1,自引:0,他引:1
Chaffey, N. J. and Harris, N. 1985. Localization of ATPase activityon the plasmalemma of scutellar epithelial cells of germinatingbarley (Hordeum vulgare L.).J. exp. Bot 36: 16121619. ATPase activity has been localized at an ultrastructural levelin the absorptive region of the scutella of germinating barley(Hordeum vulgare L.). The enzyme is localized on the plasmalemmaof the epithelial cells. Using the Gomori reaction the depositionof reaction product on the plasmalemma, which is dependent uponthe presence of supplied ATP, was precluded or reduced by theinhibitors orthovanadate, mercuric chloride and DCCD, whilstß-glycerophosphate would not act as an alternativesubstrate. The mitochondria demonstrated phosphatase activitywith both ATP and ß-glycerophosphate as substrate.The results are discussed in relation to the active uptake ofmetabolites by the scutellum during germination and the structuralmodification of the plasmalemma of the epithelial cells to formplasmatubules. Key words: ATPase, Hordeum vulgare L., localization (ultrastructural) 相似文献
3.
Root Water Uptake, Leaf Water Storage and Gas Exchange of a Desert Succulent: Implications for Root System Redundancy 总被引:3,自引:1,他引:2
A technique used for hydroponics was adapted to measure instantaneousroot water uptake from the soil for a leaf succulent CAM species,Agave deserti. Comparisons were made to previously modelledwater fluxes for A. deserti and to Encelia farinosa, a non-succulentC3species. Net CO2uptake and transpiration forA. deserti underwell-watered conditions occurred primarily at night whereasroot water uptake was relatively constant over 24 h. Leaf thicknessdecreased when transpiration commenced and then increased whenrecharge from the stem and soil occurred, consistent with previousmodels. A drought of 90 d eliminated net CO2uptake and transpirationand reduced the water content of leaves by 62%. Rewetting theentire root system for 7 d led to a full recovery of leaf waterstorage but only 56% of maximal net CO2uptake. Root water uptakewas maximal immediately after rewetting, which replenished rootwater content, and decreased to a steady rate by 14 d. Whenonly the distal 50% of the root system was rewetted, the timefor net CO2uptake and leaf water storage to recover increased,but by 30 d gas exchange and leaf water storage were similarto 100% rewetting. Rewetting 10 or 20% of the root system resultedin much less water uptake; these plants did not recover leafwater storage or gas exchange by 30 d after rewetting. A redundancyin the root system of A. deserti apparently exists for dailywater uptake requirements under wet conditions but the entireroot system is required for rapid recovery from drought.Copyright1999 Annals of Botany Company Agave deserti Engelm., desert, drought, gas exchange, rewetting, roots, succulent, water uptake. 相似文献
4.
Root Growth and Water Uptake by Maize Plants in Drying Soil 总被引:16,自引:0,他引:16
Sharp, R. E and Da vies, W. J. 1985. Root growth and water uptakeby maize plants in drying soil. J. exp. Bot. 36: 14411456. The influence of soil drying on maize (Zea mays L.) root distributionand use of soil water was examined using plants growing in thegreenhouse in soil columns. The roots of plants which were wateredwell throughout the 18 d experimental period penetrated thesoil profile to a depth of 60 cm while the greatest percentageof total root length was between 2040 cm. High soil waterdepletion rates corresponded with these high root densities.Withholding water greatly restricted root proliferation in theupper part of the profile, but resulted in deeper penetrationand higher soil water depletion rates at depth, compared withthe well watered columns. The deep roots of the unwatered plantsexhibited very high soil water depletion rates per unit rootlength. Key words: Maize, roots, water deficit, soil water depletion 相似文献
5.
The Effect of Water Stress upon Polyamine Levels in Barley (Hordeum vulgare L.) Leaves 总被引:1,自引:0,他引:1
The effect of water stress on leaf polyamine content of fourHordeum vulgare varieties, Alger/Ceres, Palmella Blue, Rihaneand Roho, with different drought characters was studied After6 d without water Alger/Ceres, Palmella Blue and Rihane hadaccumulated putrescine, although only in concentrations up totwice those found in the controls, but Roho had a decreasedputrescine content. However, one common response was identified;the accumulation and subsequent loss of putrescine was dependentupon the maintenance and loss of leaf turgor respectively. Consequentlyvarietal differences in putrescine accumulation were relatedto water consumption rates and the extent of osmotic adjustment.Spermine behaved in a similar manner to putrescine but spermidinelevels always decreased. Polyamine levels were never high enoughto be an important component of solute accumulation. Prolinelevels were 150-fold higher and glycine-betaine levels 50-foldhigher than polyamines in stressed plants. Proline and glycine-betaineaccumulation occurred once a threshold turgor was reached, whichin Roho appeared to be a reduction by 02025MPa or 3040%. The importance of polyamine accumulationduring water stress is discussed. Key words: Hordeum vulgare, polyamine, water stress 相似文献
6.
Excised embryos of Phaseolus vulgaris incubated in a mediumcontaining 10 mg dm3 farnesol showed enhanced root growthwhereas the leaves remained rudimentary At lower concentrationsof exogenous farnesol normal leaf development occurred and rootgrowth was comparable to untreated cultures. Enhanced root growthalso occurred when excised embryos of Hordeum vulgare were treatedwith farnesol but only at 10 mg dm3 and this treatmentdid not prevent leaf growth X-ray micro-probe analysis of leavesrevealed an increased phosphorus content in P vulgaris and adecreased sulphur content in H vulgare in comparison to untreatedplants. Hordeum vulgare L., barley, Phaseolus vulgaris, bean, embryo culture, farnesol, X-ray microprobe analysis, root growth 相似文献
7.
U. SCHMIDHALTER 《Plant, cell & environment》1997,20(7):953-960
Uptake of soil water by plants may result in significant gradients between bulk soil and soil in the vicinity of roots. Few experimental studies of water potential gradients in close proximity to roots, and no studies on the relationship of water potential gradients to the root and leaf water potentials, have been conducted. The occurrence and importance of pre-dawn gradients in the soil and their relation to the pre-dawn root and leaf water potentials were investigated with seedlings of four species. Pre-germinated seeds were grown without watering for 7 and lid in a silt loam soil with initial soil matric potentials of -0.02, -0.1 and -0.22 MPa. Significant gradients, independent of the species, were observed only at pre-dawn soil matric potentials lower than -0.25 MPa; the initial soil matric potentials were -0.1 MPa. At an initial bulk soil matric potential of -0.22 MPa, a steep gradient between bulk and rhizoplane soil was observed after 7 d for maize (Zea mays L. cv. Issa) and sunflower (Helianthus annuus L. cv. Nanus), in contrast to barley (Hordeum vulgare L. cv. Athos) and wheat (Triticum aestivum L. cv. Kolibri). Pre-dawn root water potentials were usually about the same as the bulk soil matric potential and were higher than the rhizoplane soil matric potential. Pre-dawn root and leaf water potentials tended to be much higher than rhizoplane soil matric potentials when the latter were lower than -0.5 MPa. It is concluded that plants tend to become equilibrated overnight with the wetter bulk soil or with wetter zones in the bulk soil. Plants can thus circumvent negative effects of localized steep pre-dawn soil matric potential gradients. This may be of considerable importance for water uptake and growth in drying soil. 相似文献
8.
Sagebrush (Artemisia tridentata Nutt.) roots maintain nutrient uptake capacity under water stress 总被引:2,自引:0,他引:2
Phosphorus and nitrogen uptake capacities were assessed during3658 d drying cycles to determine whether the abilityof sagebrush (Artemisia tridentata Nutt.) to absorb these nutrientschanged as the roots were subjected to increasing levels ofwater stress. Water was withheld from mature plants in large(6 I) containers and the uptake capacity of excised roots insolution was determined as soil water potentials decreased from0.03 MPa to 5.0 MPa. Phosphorus uptake rates of excised roots at given substrateconcentrations increased as preharvest soil water potentialsdecreased to 5.0 MPa. Vmax and Km also increased as soilwater potentials declined. Declining soil water potentials depressednitrogen uptake at set substrate concentrations, but uptakecapacity, calculated as the sum Vmax for both NH+4+NO3,did not change significantly with drying. The sum Vmax correlatedwith root nitrogen concentration. Root uptake capacity for nitrogen and phosphorus was extremelystable under severe water stress in this aridland shrub. Maintenanceof uptake capacity, coupled with a previously demonstrated abilityto conduct hydraulic lift, may enable A. tridentata better tomaintain nitrogen and phosphorus uptake as soil water availabilitydeclines. These mechanisms may be important in the ability ofA. tridentata to maintain growth, complete reproduction, andgain an advantage against competitors late in the season whenthe soil layers with higher nutrient availability are dry. Key words: Kinetics, nitrogen, phosphorus, roots, water stress 相似文献
9.
High temperatures (up to 35 °C) were applied to plants ofmalting barley,Hordeum vulgareL. (Schooner) fora period of 5 d during grain-filling. Heat treatment had a profoundeffect on the structure of the mature barley grain. There wasevidence of degradation of endosperm storage products in heat-treatedgrain. Starch granule development was reduced in sub-aleuronecells following heat treatment and alterations to starch granuledistribution and growth were observed in the endosperms of thesegrains. Endosperm cell wall and crushed cell layer (CCL) developmentwere sensitive to high temperatures, with the reduced thicknessof the CCL and generally patchy Calcofluor fluorescence of endospermcell walls indicative of partial hydrolysis of ß-glucans.Increased growth of the embryo took place in heat-treated grainscompared with control grains. Endosperm texture was generallymore friable in heat-treated grains than in control grains,and these grains overmodified during malting, with considerabledegradation of starch in the form of extensive pitting of A-typestarch granules. Evidence is presented for developmental andgermination events occurring simultaneously within the developinggrain.Copyright 1998 Annals of Botany Company Barley,Hordeum vulgareL., starch granules, crushed cell layer, scutellum, embryo, fluorescence microscopy, scanning electron microscopy, confocal microscopy, malting quality. 相似文献
10.
Hydraulic Conductances of the Soil, the Root-Soil Air Gap, and the Root: Changes for Desert Succulents in Drying Soil 总被引:5,自引:0,他引:5
Water movement to and from a root depends on the soil hydraulicconductivity coefficient (Lsoil), the distance across any root-soilair gap, and the hydraulic conductivity coefficient of the root(LP). After analytical equations for the effective conductanceof each part of the pathway are developed, the influences ofsoil drying on the soil water potential and Lsoil are describedduring a 30 d period for a loamy sand in the field. The influencesof soil drying on LP for three desert succulents, Agave deserti,Ferocactus acanthodes, and Opuntia ficus-indica, are also describedfor a 30 d period. To quantify the effects of soil drying onthe development of a root-soil air gap, diameters of 6-week-oldroots of the three species were determined at constant watervapour potentials of 1.0 MPa and 10 MPa as wellas with the water vapour potential decreasing at the same rateas soil drying during a 30 d period. The shrinkage observedfor roots initially 2·0 mm in diameter averaged 19% duringthe 30d period. The predominant limiting factor for water movementwas LP of the root for the first 7 d of soil drying, the root-soilair gap for the next 13 d, and Lsoil thereafter. Compared withthe ease of water uptake from a wet soil, the decrease in conductancesduring soil drying, especially the decrease in Lsoil causedthe overall conductance to decrease by 3 x 103-fold during the30 d period for the three species considered, so relativelylittle water was lost to the dry soil. Such rectifier-like behaviourof water movement in the soil-root system resulted primarilyfrom changes in Lsoil and, presumably, is a general phenomenonamong plants, preventing water loss during drought but facilitatingwater uptake after rainfall. Key words: Agave deserti, Ferocactus acanthodes, Opuntia ficus-indica, rectification, soil water potential, water movement 相似文献
11.
Thermal and Water Relations of Roots of Desert Succulents 总被引:6,自引:0,他引:6
Two succulent perennials from the Sonoran Desert, Agave desertiEngelm. and Ferocactus acanthodes (Lem.) Britton and Rose, loselittle water through their roots during drought, yet respondrapidly to light rainfall. Their roots tend to be shallow, althoughabsent from the upper 20 mm or so of the soil. During 1215d after a rainfall, new root production increased total rootlength by 47 per cent to 740 m for A. deserti and by 27 percent to 230 m for F. acanthodes; root dry weight then averagedonly 15 per cent of shoot dry weight. The annual carbon allocatedto dry weight of new roots required 11 per cent of shoot carbondioxide uptake for A. deserti and 19 per cent for F. acanthodes.Elongation of new roots was greatest near a soil temperatureof 30°C, and lethal temperature extremes (causing a 50 percent decrease in root parenchyma cells taking up stain) were56°C and -7°C. Soil temperatures annually exceeded themeasured tolerance to high temperature at depths less than 20mm, probably explaining the lack of roots in this zone. Attached roots immersed in solutions with osmotic potentialsabove -2·6 MPa could produce new lateral roots, with50 per cent of maximum elongation occurring near -1·4MPa for both species. Non-droughted roots lost water when immersedin solutions with osmotic potentials below -0·8 MPa,and root hydraulic conductance decreased markedly below about-1·2 MPa. Pressure-volume curves indicated that, fora given change in water potential, non-droughted roots lostthree to five times more water than droughted roots, non-droughtedleaves, or non-droughted stems. Hence, such roots, which couldbe produced in response to a rainfall, will lose the most tissuewater with the onset of drought, the resulting shrinkage beingaccompanied by reduced root hydraulic conductance, less contactwith drying soil, and less water loss from the plant to thesoil. Agave deserti, Ferocactus acanthodes, roots, soil, temperature, water stress, drought, Crassulacean acid metabolism, succulents 相似文献
12.
Use of the root contact concept,an empirical leaf conductance model and pressure-volume curves in simulating crop water relations 总被引:5,自引:0,他引:5
A simulation model “DanStress” was developed for studying the integrated effects of soil, crop and climatic conditions on
water relations and water use of field grown cereal crops. The root zone was separated into 0.1 m deep layers of topsoil and
subsoil. For each layer the water potential at the root surface was calculated by a single root model, and the uptake of water
across the root was calculated by a root contact model. Crop transpiration was calculated by Monteith's combination equation
for vapour flow. Crop conductance to water vapour transfer for use in Monteith's combination equation was scaled up from an
empirical stomatal conductance model used on sunlit and shaded crop surfaces of different crop layers. In the model, transpirational
water loss originates from root water uptake and changes in crop water storage. Crop water capacitance, used for describing
the water storage, was derived from the slope of pressure-volume (PV) curves of the leaves. PV curves were also used for deriving
crop water potential, osmotic potential, and turgor pressure. The model could simulate detailed diurnal soil-crop water relations
during a 23-day-drying cycle with time steps of one hour.
During the grain filling period in spring barley (Hordeum distichum L.), grown in a sandy soil in the field, measured and predicted values of leaf water and osmotic potential, RWC, and leaf
stomatal conductance were compared. Good agreement was obtained between measured and predicted values at different soil water
deficits and climatic conditions. In the field, measured and predicted volumetric soil water contents (θ) of topsoil and subsoil
layers were also compared during a drying cycle. Predicted and measured θ-values as a function of soil water deficits were
similar suggesting that the root contact model approach was valid.
From the investigation we concluded: (I) a model, which takes the degree of contact between root surface and soil water into
account, can be used in sandy soil for calculation of root water uptake, so that the root conductance during soil water depletion
only varies by the degree of contact; (II) crop conductance, used for calculation of crop transpiration, can be scaled up
from an empirical single leaf stomatal conductance model controlled by the level of leaf water potential and micrometeorological
conditions; (III) PV curves are usable for describing crop water status including crop water storage. 相似文献
13.
Barley plants (Hordewn vulgare L. cv. Atem) were grown fromseed for 28 d in flowing solution culture, during which timeroot temperature was lowered decrementally to 5?C. Plants werethen subjected to root temperatures of 3, 5, 7, 9, 11, 13, 17or 25 ?C, with common air temperature of 25/15 ?C (day/night).Changes in growth, plant total N, and NO3 levels, andnet uptake of NH4+ and NO3 from a maintained concentrationof 10 mmol m3 NH4NO3 were measured over 14 d. Dry matterproduction increased 6-fold with increasing root temperaturebetween 325 ?C. The growth response was biphasic followingan increase in root temperature. Phase I, lasting about 5 d,was characterized by high root specific growth rates relativeto those of the shoot, particularly on a fresh weight basis.During Phase I the shoot dry weight specific growth rates wereinversely related to root temperature between 313 ?C.Phase 2, from 514 d, was characterized by the approachtowards, and/or attainment of, balanced exponential growth betweenshoots and roots. Concentrations of total N in plant dry matterincreased with root temperature between 325 ?C, moreso in the shoots than roots and most acutely in the youngestfully expanded leaf (2?l6?9% N). When N contents wereexpressed on a tissue fresh weight basis the variation withtemperature lessened and the highest concentration in the shootwas at 11 ?C. Uptake of N increased with root temperature, andat all temperatures uptake of NH4+, exceeded that of NO3,irrespective of time. The proportions of total N uptake over14 d absorbed in the form of NH4+ were (%): 86, 91, 75, 77,76, 73, 77, and 80, respectively, at 3, 5, 7, 9, Il, 13, 17,and 25 ?C. At all temperatures the preference for NH4+ overNO3 uptake increased with time. An inverse relationshipbetween root temperature (311 ?C) and the uptake of NH4+as a proportion of total N uptake was apparent during PhaseI. The possible mechanisms by which root temperature limitsgrowth and influences N uptake are discussed. Key words: Hordeum vulgare, root temperature, ammonium, nitrate, ion uptake, growth rate 相似文献
14.
Resource Capture by Localized Root Proliferation: Why Do Plants Bother? 总被引:12,自引:0,他引:12
Using data from a well-known, published experiment [Drew (1975)TheNew Phytologist75: 479490], the potential exploitationof locally available nutrients by barley roots is calculated.Local proliferation of lateral roots does not necessarily achievesignificantly greater exploitation of mobile soil resourceslike nitrate, but it does for less mobile ones such as phosphate.Despite this, the magnitude of the proliferative response isas great to locally available nitrate as it is to phosphate.This implies an over-production of roots in responseto localized nitrate availability, prompting a re-evaluationof the nature and implications of the response mechanism(s)of roots to soil heterogeneity. Hordeum vulgare; barley; carbon; heterogeneity; lateral root; nitrate; localized nutrient supply; phosphate; proliferation; root 相似文献
15.
Water uptake and structural plasticity along roots of a desert succulent during prolonged drought 总被引:3,自引:0,他引:3
Desert succulents resume substantial water uptake within 1–2 d of the cessation of drought, but the changes in root structure and hydraulic conductivity underlying such recovery are largely unknown. In the monocotyledonous leaf succulent Agave deserti Engelm. substantial root mortality occurred only for lateral roots near the soil surface; nearly all main roots were alive at 180 d of drought. New main roots were initiated and grew up to 320 mm at soil water potentials lower than – 5·0 MPa, utilizing water from the shoot. The hydraulic conductivity of distal root regions decreased 62% by 45 d of drought and 70% thereafter. After 7 d of rewetting, root hydraulic conductivity was restored following 45 d of drought but not after 90 and 180 d. The production of new lateral roots and the renewed apical elongation of main roots occurred 7–11 d after rewetting following 180 d of drought. Hydraulic conductivity was higher in the distal region than at midroot and often increased again near the root base, where many endodermal cells lacked suberin lamellae. Suberization and xylem maturation were influenced by the availability of moisture, suggesting that developmental plasticity along a root allows A. deserti to capitalize on intermittent or heterogeneous supplies of water. 相似文献
16.
Water extraction from subsoil in upland rice (Oryza sativa L.) was examined as related to topsoil desiccation and subsoil compaction. The water extraction was observed by measurements of heavy water concentrations in transpiring plants. The plants were grown in pots that were filled with sandy soil and vertically compartmented into two columns. Heavy water was applied to the subsoil. Plants exposed to mild topsoil desiccation (–120 kPa in water potential) eventually increased water extraction from the subsoil and maintained photosynthetic rate and stomatal conductance at the wet condition level. The rates of the plants subjected to severely droughted topsoil (–190 kPa) were significantly lowered due to less water uptake from the subsoil. Subsoil compaction at bulk densities of 1.45 and 1.50 Mg m–3 inhibited increase of root length densities. Limited water extraction from the subsoil was insufficient to maintain plant productivity under drought conditions. Daily water uptake per unit of root length in the lower tube did not apparently increase even if water demand on the unit root length increased. When water to topsoil was completely withheld, water extraction from the subsoil gradually increased as the topsoil dried out. Plants that were watered and rewatered took up very little water from the subsoil. The extraction from the subsoil occurred only when water potential of the topsoil was below about –190 kPa. 相似文献
17.
A Two-dimensional Model for Water Uptake by Desert Succulents: Implications of Root Distribution 总被引:3,自引:0,他引:3
Water uptake by Agave deserti and Ferocatus acanthodes was predictedusing a two-dimensional simulation model in which the soil arounda plant was divided into a series of layers and concentric cylindricalshells. Root lengths in 0.05 m thick soil layers were determinedfor both species in the field, where mean root depths were only0.11 m for A. deserti and 0.10 m for F. acanthodes. For a yearwith average precipitation (159 mm), 42 per cent of the annualprecipitation could be taken up by A. deserti and 25 per centby F. acanthodes. Predicted water uptake by both species wasgreater from the upper soil layers (above 0.15 m) for averageand dry years, but was greater from the deeper layers for awet year. The actual root distribution for both species ledto more water uptake than when all of the roots were in a singlelayer. The large number of days per year when the soil temperaturesexceeded 57 °C (the temperature for 50 per cent inhibitionof uptake of a vital stain by root cells) may exclude rootsfrom the 0.000.05 m soil layer, even though water uptakewhen all roots were located there was predicted to be maximal.Therefore, the observed root distribution of A. deserti andF. acanthodes may be limited near the soil surface by high temperaturesand at maximum depths by water availability for all but wetyears. Agave deserti, Ferocactus acanthodes, desert succulents, root system, root distribution, soil temperature, water uptake 相似文献
18.
Relationships between nitrate (NO-3) supply, uptake and assimilation,water uptake and the rate of mobilization of seed reserves wereexamined for the five main temperate cereals prior to emergencefrom the substrate. For all species, 21 d after sowing (DAS),residual seed dry weight (d.wt) decreased while shoot plus rootd.wt increased (1530%) with increased applied NO-3concentrationfrom 0 to 520 mM . Nitrogen (N) uptake and assimilationwere as great with addition of 5 mM ammonium (NH+4) or 5 mMNO-3but NH+4did not affect the rate of mobilization of seedreserves. Chloride (Cl-) was similar to NO-3in its effect onmobilization of seed reserves of barley (Hordeum vulgare L.).Increased rate of mobilization of seed reserves with additionalNO-3or Cl-was associated with increases in shoot, root and residualseed anion content, total seedling water and residual seed watercontent (% water) 21 DAS. Addition of NH+4did not affect totalseedling water or residual seed water content. For barley suppliedwith different concentrations of NO-3or mannitol, the rate ofmobilization of seed reserves was positively correlated (r >0.95)with total seedling water and residual seed water content. Therate of mobilization of seed reserves of barley was greaterfor high N content seed than for low N content seed. Seed watercontent was greater for high N seed than for low N seed, 2 DAS.Additional NO-3did not affect total seedling water or residualseed water content until 1014 DAS. The effects of seedN and NO-3on mobilization of seed reserves were detected 10and 14 DAS, respectively. It is proposed that the increasedrate of mobilization of seed reserves of temperate cereals withadditional NO-3is due to increased water uptake by the seedlingwhile the seed N effect is due to increased water uptake bythe seed directly. Avena sativa L.; oat; Hordeum vulgare L.; barley; Secale cereale L.; rye; xTriticosecale Wittm.; triticale; Triticum aestivum L.; wheat; nitrate; seed; germination; seed reserve mobilization 相似文献
19.
Drought Influences the Activity of Enzymes of the Chloroplast Hydrogen Peroxide Scavenging System 总被引:12,自引:2,他引:10
Smirnoff, N. and Colomb?, S. V. 1988. Drought influences theactivity of enzymes of the chloroplast hydrogen peroxide scavengingsystem.J. exp. Bot. 39: 10971108. The effect of drought on the activity of ascorbate peroxidase(AP), glutathione reductase (GR) and monodehydroascorbate reductase(MDAR) in leaves of barley (Hordeum vulgare) and tef {Eragrostistef) was studied. These enzymes are components of the chloroplasthydrogen peroxide scavenging system. Severe leaf water deficit(<30 M Pa) resulted in increased activity (leaf dryweight basis) of GR and MDAR in barley and of AP and MDAR intef. The specific activity of all the scavenging enzymes wasgreater in droughted plants. The activities (dry weight basis)of two control enzymes, malate dehydrogenase (barley)and phosphoenolpyruvate carboxylase (tef), not directly involvedin the scavenging system, were not affected by drought. These data suggest that drought may cause an increase in thecapacity of the hydrogen peroxide scavenging system and thatit may, therefore, increase the rate of hydrogen peroxide formationin chloroplasts. Key words: Water stress, hydrogen peroxide, enzyme activity 相似文献
20.
Culture of Barley Anthers in Conditioned Media 总被引:2,自引:1,他引:1
High yielding anther cultures of Hordeum vulgare cv. Sabarlisare obtained at inoculation densities of 1020 per mlby use of media previously conditioned by Sabarlis anthers.To achieve these high yields anthers at the mid-unicellularpollen stage (stage 2), stressed in the excised spike for 14d at 7 °C, are necessary, but for conditioning of mediaolder anthers may be used with or without the stress pretreatment.Conditioning for 7 d by anthers at young bicellular pollen stages(stages 56) is highly effective. Hormones supplied inthe medium interact synergistically with the conditioning factor. Sabarlis ovaries are shown to be even more effective for conditioningthan anthers, whereas glumes and other parts of the spike arerelatively ineffective. Anthers of oats, rye, wheat, maize,tobacco, and one other genotype of barley are also less effectivethan Sabarlis anthers. The improved method of anther culture is more efficient thanspike culture for the production of pollen callus in Sabarlisbarley. 相似文献