工业生物催化技术在绿色精细化学品合成中的机遇

生物催化技术具有反应条件温和、专一性强、环境友好等特点,是传统化学方法无法比拟的。本文从生物催化技术的特点及其在精细化学品合成中的应用现状出发,从产品战略、技术策略等方面提出生物催化技术应用的方向与目标：生物催化与有机合成技术的集成、交叉将会是未来生物催化技术发展的重要方向;有机合成技术为生物催化技术的发展、应用指明并提供了更多技术、产品及产业机会。     

生物反应器技术平台及其在新药研发中的应用

<正>生物反应器是科研成果的转化及新药研发的重要技术平台。简要介绍长江学者蛋白药物重点科技创新团队在多种生物反应器技术平台的建立及其在新药研发中的应用,为以高校为科研主体,开展新型"产学研"相结合的模式,积极推动科研成果的产业转化提供一种可借鉴模式。     

基于微流控芯片的细胞-细菌相互作用光电检测技术

细胞/细菌及其相互作用研究对于生命科学、药物研发、医学诊疗等领域的研究具有重要意义。微流控芯片分析技术因微环境可控、生物相容性好、检测并行性、微型化等特性，正发展成为细胞/细菌及其相互作用研究的高效手段。本文在简要介绍基于微流控芯片分析技术的细胞-细菌分析方法和技术基础之上，对微流控芯片上细胞-细菌相互作用模型的建立进行了讨论，重点针对细胞-细菌及其相互作用过程的芯片检测进行了综述，尤其对芯片集成的光电检测技术及其测试效果进行总结和比较。通过芯片集成微流体控制、多种光电传感监测模块，使微流控芯片分析技术成为细胞/细菌及其相互作用过程分析和检测的支撑平台和优势手段。最后，对微流控光电检测技术在细胞-细菌相互作用检测中面临的挑战及发展趋势进行了讨论和展望。     

《生物产业技术》创刊五周年回顾之医药生物技术篇

医药生物技术产业是生物技术产业最重要的组成部分,是《生物产业技术》杂志重点关注领域之一。《生物产业技术》自2005年创刊以来,一直追踪医药生物行业的热点、关注医药生物行业的发展,期待将医药生物产业的精彩展示给产业界和大众。杂志通过“公司新闻”、“前沿技术”、“新药连载”、”专题”等多种形式记录了医药生物产业研发以及医药生物制造企业、研发机构、合同研发外包企业、投资机构等发展实践历程。     

黄土高原水土流失综合治理技术及示范

黄土高原是我国水土流失严重和生态环境极为脆弱的地区,也是我国"两屏三带"生态安全战略格局的重要组成部分。研发及集成相关技术,建立试验示范样板,为该区生态修复与产业发展提供技术支撑成为亟待解决的重要问题。"黄土高原水土流失综合治理技术及示范"项目(2016YFC0501700)属于国家重点研发计划"典型脆弱生态修复与保护研究"专项。该项目通过6个类型区水土流失治理相关技术的研究及区域尺度上的集成研发,阐明黄土高原脆弱区域生态持续恢复与生态安全中的主要学科发展及相关技术问题。     

手性技术与生物催化

简要介绍了手性,手性技术与生物催化的基本概念。手性,是指一个有机分子具有不对称性,形成两种空间排布方式不同的对映异构体。手性技术即生产手性化合物的技术,手性化合物的制备方法主要有手性源、外消旋体拆分、不对称合成等几种。生物催化,即利用酶或微生物等生物材料催化进行某种化学反应,被认为是手性化合物生产取得突破的关健技术。文章还介绍了生物催化外消旋体拆分、生物催化不对称合成等几种生产手性化合物的应用实例。     

《生物产业技术》征稿简则

《生物产业技术》（CNll—5606／Q．ISSNl674一0319．邮发代号80～627）立足生物产业．关注技术进步．面向生物农业、生物医药、生物制造、生物能源以及生物环保等领域的技术研发、工业生产、市场营销以及经营管理等环节．宣传国家相关产业政策．发布相关技术、生产、市场、产品等信息．帮助企业进行正确的经营管理决策,推动国家生物技术产业的快速发展．具有较强的技术性、应用性和信息性。     

《生物产业技术》征稿简则

《生物产业技术》（CN11-5606／Q,ISSN1674—0319,邮发代号80-627）立足生物产业,关注技术进步,面向生物农业、生物医药、生物制造、生物能源以及生物环保等领域的技术研发、工业生产、市场营销以及经营管理等环节,宣传国家相关产业政策,发布相关技术、生产、市场、产品等信息,帮助企业进行正确的经营管理决策,推动国家生物技术产业的快速发展,具有较强的技术性、应用性和信息性。     

《生物产业技术》征稿简则

《生物产业技术》（CN11-5606／O,ISSN1674—0319,邮发代号80—627）立足生物产业,关注技术进步,面向生物农业、生物医药、生物制造、生物能源以及生物环保等领域的技术研发、工业生产、市场营销以及经营管理等环节,宣传国家相关产业政策,发布相关技术、生产、市场、产品等信息,帮助企业进行正确的经营管理决策,推动国家生物技术产业的快速发展,具有较强的技术性、应用性和信息性。     

《生物加工过程》2006年总目次

第1期目次专论与综述以技术集成促进生物催化技术转移……………………………………杨晟,杨蕴刘,袁中一,姜卫红(1)化学修饰———提高酶催化性能的重要工具………………………………………………张松平,王平(4)酶定向进化的研究进展………………………………………………………     

光催化与纯紫外工艺对船舶压载水微藻的处理效果比较

分析光催化和纯紫外工艺对控制处理压载水中10～50μm藻类的影响,比较了压载水中10～50μm藻类的去除率、种类变化、绿藻和硅藻去除率以及硅藻种类数的变化,结果显示光催化工艺对10～50μm藻类的去除效果要好于纯紫外工艺。两种工艺对10～50μm藻类的即时去除率均可以达到96.0%以上,压载数日后排放则效果更佳。研究发现两种工艺对硅藻的处理效果不如绿藻。并且压载水盐度会影响压载水处理工艺的效果。     

The historical development of vaccine technology: exploring the relationship between science and technology

This paper examines the feasibility of using historical case studies to contextualise the learning of the nature of science and technology in a biology lesson. Through exploring the historical development of vaccine technology, students were expected to understand the complexity of the relationships between technology and science beyond the simplistic portrayal of technology as ‘applied science’. Instructional scaffolding in the form of Socratic Dialogue and self-reflection was used to engage students in thinking about the difference in the nature of science and technology and their mutual interactions. This was followed by students’ reflections on the insights they have gained from the lesson as a means to evaluate and reconstruct their own understanding if necessary. The educational implications of the findings are discussed to explore how technology could be further integrated into the school science curriculum to enhance scientific and technological literacy.     

Microchip technology applications for blood group analysis

Blood group analysis techniques are some of the most in demand immunological applications in clinical transfusion praxis and organ transplantation. In order to aid the advance towards higher throughput and increased sensitivity, analytical solutions dealing with a minimal amount of blood samples and the miniaturization of diagnostic equipment using microchip technologies have been evolving into an optimal solution. Here we review fabrication technologies for various types of microstructure on microchips, related operating procedures, and characterization approaches. Our focus is on examples of microchip technology and instrumentation used for blood group analysis ranging from classical serological methods of glycoprotein detection and solid phase assays, to nucleic acid amplification techniques. Molecular typing using microchip-based techniques is emerging as a supplement to standard serological methods. Microchip technology will play its key role to support blood group analysis at the molecular scale by using microliters of blood samples for extremely sensitive, quantitative, and high throughput analyses.     

3S技术与生物多样性研究

生物多样性是地球生物圈和人类发展的基础,是国际社会所共同关注的焦点问题。3S技术是集遥感、全球定位与地理信息系统于一体的高新技术手段,为生物多样性研究提供强有力的技术支持。近年来该领域的研究越来越受到人们的重视,并取得了大量的研究成果。     

基因人工进化的分子育种技术

分子育种技术(molecular breeding technology)利用人工手段模拟生物杂交优势的分子进化模式,在体外构建含有感兴趣基因的大量突变文库,并根据基因表达产物的特性,以合适的筛选方法选择具有最佳功能的改良基因。经过近几年的快速发展,分子育种技术在创造高效随机基因突变库的方法上日臻完善,已在医学、工业和环境保护酶类研究等方面收到了很好的成效。预计未来该技术将广泛地应用于重要传染性疾病(如疟疾、艾滋病病毒等)的预防和治疗,尤其可通过DNA疫苗手段的应用而获得强大的生命力。作者全面地综述了分子育种技术的产生、发展和应用现状。     

Vaccine process technology

The evolution of vaccines (e.g., live attenuated, recombinant) and vaccine production methods (e.g., in ovo, cell culture) are intimately tied to each other. As vaccine technology has advanced, the methods to produce the vaccine have advanced and new vaccine opportunities have been created. These technologies will continue to evolve as we strive for safer and more immunogenic vaccines and as our understanding of biology improves. The evolution of vaccine process technology has occurred in parallel to the remarkable growth in the development of therapeutic proteins as products; therefore, recent vaccine innovations can leverage the progress made in the broader biotechnology industry. Numerous important legacy vaccines are still in use today despite their traditional manufacturing processes, with further development focusing on improving stability (e.g., novel excipients) and updating formulation (e.g., combination vaccines) and delivery methods (e.g., skin patches). Modern vaccine development is currently exploiting a wide array of novel technologies to create safer and more efficacious vaccines including: viral vectors produced in animal cells, virus-like particles produced in yeast or insect cells, polysaccharide conjugation to carrier proteins, DNA plasmids produced in E. coli, and therapeutic cancer vaccines created by in vitro activation of patient leukocytes. Purification advances (e.g., membrane adsorption, precipitation) are increasing efficiency, while innovative analytical methods (e.g., microsphere-based multiplex assays, RNA microarrays) are improving process understanding. Novel adjuvants such as monophosphoryl lipid A, which acts on antigen presenting cell toll-like receptors, are expanding the previously conservative list of widely accepted vaccine adjuvants. As in other areas of biotechnology, process characterization by sophisticated analysis is critical not only to improve yields, but also to determine the final product quality. From a regulatory perspective, Quality by Design (QbD) and Process Analytical Technology (PAT) are important initiatives that can be applied effectively to many types of vaccine processes. Universal demand for vaccines requires that a manufacturer plan to supply tens and sometimes hundreds of millions of doses per year at low cost. To enable broader use, there is intense interest in improving temperature stability to allow for excursions from a rigid cold chain supply, especially at the point of vaccination. Finally, there is progress in novel routes of delivery to move away from the traditional intramuscular injection by syringe approach.     

益生菌微胶囊化技术研究概述

本文介绍了益生菌的生理功能及其微胶囊化的不同方法,并探讨了其发展前景。     

转基因技术与大豆品质改良

大豆是重要的食用油、食用蛋白和饲用蛋白原料。应用转基因技术对调节大豆品质形成的关键基因进行定向操作,可大大加速优质大豆品种的选育进程,培育出适合不同消费需求的多样化优质大豆品种。文章主要综述了近年来转基因技术在大豆品质性状改良方面的应用进展,同时也简要介绍了目前已经发展起来的安全转基因技术。     

Quantification of phosphoproteins with global internal standard technology

Global internal standard technology (GIST) is being developed for the quantification of all primary structure and post-translational variants of proteins in a proteome. This paper is directed at an analysis of phosphorylation, primarily of serine and threonine. Quantification was achieved by acylation of primary amino groups in peptide cleavage fragments of proteins with isotopically coded derivatizing agents. Peptides from controls were globally coded with an isotopically "light" form of the reagent while those from experimental samples were coded with a "heavy" form of the reagent. The two types coding reagents used in this work were N-hydroxyl succinimide derivatives of acetate and 4-trimethylammoniumbutyrate. Heavy isotope forms were produced by deuteration of methyl groups. Subsequent to coding and mixing, the two samples were passed through a Ga(III) immobilized metal affinity chromatography (IMAC) column and the selected peptide fraction was further resolved by reversed-phase chromatography (RPC) and analyzed by mass spectrometry (MS). Relative differences in phosphopeptide concentration between samples were derived from isotope ratio measurements of the peptide isoforms observed in mass spectra. The method was validated with model peptides.     

白蚁毒饵诱杀技术研究进展

毒饵技术(baiting technology)是一项古老而又有新意的白蚁防治技术,在白蚁的防治中起了重要作用。近年来毒饵技术的研究与应用取得了突破性进展。文中就国内外白蚁毒饵诱杀药剂及技术的研究与应用进行了综述。