共查询到20条相似文献,搜索用时 31 毫秒
1.
Carla Hurt Bernard Kuhajda Alexis Harman Natalie Ellis Mary Nalan 《Conservation Genetics》2017,18(6):1347-1358
The Barrens Topminnow (Fundulus julisia) has undergone a rapid and dramatic decline. In the 1980s, at least twenty localities with Barrens Topminnows were known to exist in the Barrens Plateau region of middle Tennessee; currently only three areas with natural (not stocked) populations remain. The long-term survival of the Barrens Topminnow will depend entirely on effective management and conservation efforts. Captive propagation and stocking of captive-reared juveniles to suitable habitats have successfully established a handful of self-sustaining populations. However, very little is known about the genetic composition of source and introduced populations including levels of genetic diversity and structuring of genetic variation. Here we use both mitochondrial sequence data and genotypes from 14 microsatellite loci to examine patterns of genetic variation among ten sites, including all sites with natural populations and a subset of sites with introduced (stocked) populations of this species. Mitochondrial sequence analysis reveals extremely low levels of variation within populations and fixed differences between drainages. Microsatellite genotype data shows higher levels of genetic variability and a molecular signature consistent with a recent history of population bottlenecks. Measures of genetic diversity at microsatellite loci including allelic richness are similar within source and introduced populations. Bayesian assignment tests and analysis of molecular variation (AMOVA) support two distinct populations, consistent with drainage boundaries. Results from AMOVA analysis also suggest low levels of genetic connectivity between isolated populations within the same drainage. Here we propose two distinct evolutionary significant units (ESUs) and two management units that reflect this population substructure and warrant consideration in future management efforts. 相似文献
2.
Extremely low nucleotide polymorphism in Pinus krempfii Lecomte,a unique flat needle pine endemic to Vietnam
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Wei Lun Ng Junko Kusumi Hai Hong Phi Nobuyuki Inomata Xiao‐Ru Wang Alfred E. Szmidt 《Ecology and evolution》2014,4(11):2228-2238
Pinus krempfii Lecomte is a morphologically and ecologically unique pine, endemic to Vietnam. It is regarded as vulnerable species with distribution limited to just two provinces: Khanh Hoa and Lam Dong. Although a few phylogenetic studies have included this species, almost nothing is known about its genetic features. In particular, there are no studies addressing the levels and patterns of genetic variation in natural populations of P. krempfii. In this study, we sampled 57 individuals from six natural populations of P. krempfii and analyzed their sequence variation in ten nuclear gene regions (approximately 9 kb) and 14 mitochondrial (mt) DNA regions (approximately 10 kb). We also analyzed variation at seven chloroplast (cp) microsatellite (SSR) loci. We found very low haplotype and nucleotide diversity at nuclear loci compared with other pine species. Furthermore, all investigated populations were monomorphic across all mitochondrial DNA (mtDNA) regions included in our study, which are polymorphic in other pine species. Population differentiation at nuclear loci was low (5.2%) but significant. However, structure analysis of nuclear loci did not detect genetically differentiated groups of populations. Approximate Bayesian computation (ABC) using nuclear sequence data and mismatch distribution analysis for cpSSR loci suggested recent expansion of the species. The implications of these findings for the management and conservation of P. krempfii genetic resources were discussed. 相似文献
3.
The gorgonian Paramuricea clavata is a benthic organism often included in conservation management plans since it creates complexity in the ecosystems and is
extremely vulnerable to disturbances. Eight microsatellite markers isolated from an enriched genomic library were characterized
in a total of 50 individuals from two north-western Mediterranean populations. All loci were polymorphic and the number of
alleles per locus ranged between 2 and 14. Significant genetic differentiation was observed between populations. The polymorphic
markers presently isolated will allow for the assessment of the spatial genetic structure between and within populations of
this umbrella species of the Mediterranean Sea. 相似文献
4.
Paula Feliciano de Lima Flávio Nunes Ramos Maria Imaculada Zucchi Milene Möller Regina Helena Geribello Priolli Carlos Augusto Colombo Vera Nisaka Solferini 《Conservation Genetics》2009,10(6):1883-1886
The shrub species Psychotria tenuinervis (Rubiaceae) is native to the Brazilian Atlantic forest and is largely found within natural and disturbed forest fragments.
Aiming to develop studies on population genetic structure of forest fragment species, eigth microsatellite markers were developed
for P. tenuinervis. Also, 15 loci already developed for Coffea (Rubiaceae) were tested for transferability to this species. We utilized 45 individuals from natural populations of three
different fragments-anthropic edge, interior fragment and natural edge, within the Brazilian Atlantic forest. The average
number of alleles per locus was 2.5 (two–four alleles/locus). These loci will be useful for future population genetic studies
aiming to the conservation and management of this species. 相似文献
5.
Geographic variation in the Pine Barrens Treefrog (Hyla andersonii): concordance of genetic,morphometric and acoustic signal data
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Delimiting species is important to every subfield in biology. Templeton's cohesion species concept uses genetic and ecological exchangeability to identify sets of populations that ought to be considered as the same species, and the lack of exchangeability helps determine which populations can be grouped as evolutionarily significant units (ESU) in conservation science. However, previous work assessing genetic and ecological interchangeability among populations has been limited in scope. Here, we provide a method for assessing exchangeability that incorporates multiple, independent lines of multivariate evidence in genetic, behavioural and morphological data. We use this approach to assess exchangeability across three disjunct groups of populations of the Pine Barrens Treefrog (Hyla andersonii) from the eastern United States. This species is considered threatened by each state in which it occurs and conservation management of this taxon requires a clearer understanding of how populations in these three regions may differ from one another. We find a strikingly concordant pattern in which the first axis of variation for each of the three types of data distinguishes populations along a latitudinal gradient and the second axis distinguishes the set of populations occurring in the Carolinas from those occurring in the New Jersey and Florida/Alabama regions. We know of no comparable data set that displays such concordance among different types of data across so large a geographic range. The overlap in trait values (i.e. exchangeability) between neighbouring regions, however, is substantial in all three types of data, which supports continued consideration of this taxon as a single species. 相似文献
6.
The Yangtze sturgeon (Acipenser dabryanus) is an endemic species in China. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 Mbp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species Acipenser sturio and Acipenser sinensis. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species. 相似文献
7.
Angelica sinensis (Apiaceae) is an endangered alpine herb that is widely used as a medicinal plant in traditional Chinese medicine (TCM). Wild populations of A. sinensis have become quite rare in China. Thus, population genetics studies of this species are urgently needed for its effective conservation and sustainable use. However, to date, no microsatellite loci have been isolated in A. sinensis. To address this issue, we isolated 18 polymorphic loci and genotyped 120 individuals collected from 6 populations. The number of alleles per locus ranged from 1.2 to 5.5, and the average was 2.4. The observed and expected heterozygosity per locus for a population varied, respectively, from 0.000 to 0.983 (averaged at 0.198) and from 0.066 to 0.661 (averaged at 0.333). Deviation from the Hardy–Weinberg equilibrium (p < 0.01) was observed for 4 to 14 loci in various populations. These microsatellite markers were cross-amplified in 10 species affinis, and 7 loci were successfully amplified in all species. These microsatellite markers are useful for genetic studies, the conservation management of A. sinensis, and identification of A. sinensis. 相似文献
8.
Matthew A. Gitzendanner Carl W. Weekley Charlotte C. Germain-Aubrey Douglas E. Soltis Pamela S. Soltis 《Conservation Genetics》2012,13(1):223-234
Genetic data are often crucial for designing management strategies for rare and endangered species. Ziziphus celata is an endangered sandhill shrub endemic to the Lake Wales Ridge of central Florida. This self-incompatible clonal species
is known from only 14 wild populations, most of which are small (under 100 plants). Focusing on the five populations discovered
in 2007, we evaluate the level of genetic diversity and identify clonal lineages within the wild populations of the species
with a set of microsatellite loci. To account for somatic mutations and genotyping errors, we identified clonal lineages using
a threshold cutoff for pair-wise genetic distances among samples. The microsatellites had up to 18 alleles/locus, and, consistent
with outcrossing, samples were highly heterozygous (average population level H
o
= 0.69). Most populations of Z. celata consist of a single clone, and the most diverse population has only 10 clones. Overall Z. celata comprises 41 multi-locus genotypes, and 30 clonal lineages. With nearly 1,000 recorded plants (595 genotyped) and only 30
clonal lineages, Ziziphus celata is highly clonal: clonal richness, R = 0.049. The pair-wise distance method facilitates identification of clonal lineages, avoiding overestimation of clonal diversity.
In most cases, the samples that grouped into a lineage were one to four plants differing from a surrounding genotype by a
single microsatellite repeat insertion/deletion mutation, consistent with these having arisen via somatic mutations. Our data
will enable managers to incorporate extant diversity from wild populations into ex situ collections. Additionally, our research
demonstrates the utility of microsatellites for conservation of imperiled species, identifying genotypes of high priority
for preservation. 相似文献
9.
Squid populations are being put under rapidly increasing commercial fishing pressure worldwide. The same species are known to be susceptible to extreme population fluctuations, so detailed knowledge of population substructuring and genetic diversity is essential for rational management. We present a set of microsatellite DNA loci suitable for population genetic analysis of Loligo gahi, the squid species subject to the most detailed monitoring and fishery control (around the Falkland Islands), with the future aim of generating management‐related information to aid conservation of this valuable natural resource. 相似文献
10.
ERIC T. SIMANDLE MARY M. PEACOCK LAURA ZIRELLI C. RICHARD TRACY 《Molecular ecology resources》2006,6(1):116-119
The four species that comprise the Bufo boreas group of toads are critically imperiled in all or portions of their geographical ranges. We present data from 16 microsatellite loci isolated from B. boreas that cross‐amplify in these four species. These markers have proven useful in the analyses of population structure and conservation genetics, and provide a powerful tool for future researchers who seek to understand the conservation genetics of these rare toad species. Polymorphism was assessed for 339 individuals from seven populations representing the four species. All loci were polymorphic (X? = 8, range of four to 19 alleles). Three loci were not in Hardy–Weinberg equilibrium (HWE) in one population, and one of these loci was out of HWE in a second population (P < 0.003 after Bonferroni correction for multiple tests). However, there were no systematic deviations from HWE across all study populations. Small populations in fragmented habitat appear to explain the existing HWE deviations. 相似文献
11.
Six microsatellite loci were identified and characterized in the eastern form of the widespread and commercially important giant freshwater prawn (Macrobrachium rosenbergii). The loci were detected by randomly screening for dinucleotide and trinucleotide repeat units within a partial genomic library developed for the species. In a sample of 29 prawns, number of alleles and heterozygosity per locus ranged from 12 to 18 and from 0.66 to 0.90, respectively. These markers provide powerful tools for the conservation and management of wild stocks, the improvement of cultured stocks of M. rosenbergii, and for investigating evolutionary processes underlying genetic divergence among populations. 相似文献
12.
The razor clam (Sinonovacula constricta) is an important aquacultured bivalve in China. The natural populations of this species are decreasing quickly. To facilitate
studies on genetic diversity and population structure of wild populations, microsatellites were isolated from a CA enriched
genomic library. Eight microsatellite loci were polymorphic in 30 individuals from Chongming in Shanghai, China. The number
of alleles per polymorphic locus varied from 6 to 13 and the values of observed heterozygosity and expected heterozygosity
ranged from 0.350 to 1.000 and from 0.602 to 0.902, respectively. These microsatellites are being used in studying population
differentiation and genetic diversity for effective conservation and management genetic resources of S. constricta. 相似文献
13.
Emmanouella Vogiatzi Eleni Kalogianni Brian Zimmerman Sofia Giakoumi Roberta Barbieri Peristera Paschou Antonios Magoulas Dimitris Tsaparis Nikos Poulakakis Costas S. Tsigenopoulos 《Biological journal of the Linnean Society. Linnean Society of London》2014,111(2):334-349
The genetic variation of the critically endangered Corfu killifish (Valencia letourneuxi), an endemic freshwater fish species of the western Balkans, was assessed for nine populations sampled in eight water systems in western continental Greece, the Peloponnese and the Ionian Island of Corfu, using mitochondrial and microsatellite markers. The analyses were based on data from three mtDNA regions (D‐loop, COI and 16S rRNA sequences) and 14 microsatellite loci. Samples from the congeneric species Valencia hispanica and the phylogenetically closely related species Aphanius fasciatus were also used in the study as outgroups. Both the mitochondrial and the microsatellite analyses revealed three distinct population groupings associated with the geographical distribution of the populations: one southern group occupying rivers draining to the Patraikos Gulf, the second one including the populations flowing into the Amvrakikos Gulf and the third, more northern group, including the other populations from rivers in Corfu Island and Epirus flowing into the Ionian Sea. Within these groupings there is limited genetic differentiation between populations; in addition, there is reduced intrapopulation genetic variation, evidenced by low heterozygosity values, number of alleles and haplotype diversity. In terms of taxonomic implications and appropriate management actions for conservation, our data suggest that the major population groups should be regarded at least as three distinct conservation units (CUs), with translocation and restocking actions to take place only within the geographical range of the CU concerned. © 2013 The Linnean Society of London, Biological Journal of the Linnean Society, 2014, 111 , 334–349. 相似文献
14.
Paternity determination in captive lowland gorillas and orangutans and wild mountain gorillas by microsatellite analysis 总被引:1,自引:0,他引:1
Dawn Field Leona Chemnick Martha Robbins Karen Garner Oliver Ryder 《Primates; journal of primatology》1998,39(2):199-209
Paternity exclusion studies provide useful information for testing certain theories of behavioral ecology and for the management
and conservation of both wild and captive populations of endangered species. This study used eight human nuclear microsatellite
loci, in the absence of species-specific PCR primers, to genetically identify the sires of 12 captive lowland gorillas (Gorilla gorilla gorilla) and 2 captive orangutans (Pongo pygmaeus pygmaeus andPongo p. abelii). Parentage assignments were confirmed by excluding all except a single potential sire for each offspring with the least
two loci. Sire-offspring relationships were verified in 12 of the 14 cases, and reassigned in the case of two gorilla offspring.
The orangutan paternity typing was supplemented by DNA fingerprinting. Additionally, five of the eight microsatellite loci,
in conjunction with behavioral data, were used for a non-exhaustive set of paternity exclusions for five wild mountain gorillas
(Gorilla g. beringei). The eight loci described in this study should be useful additions to the tools available for the study of genetics in the
great apes. 相似文献
15.
We constructed a genomic library and developed 14 polymorphic loci to assess species boundaries between previously described
mtDNA lineages and to examine the genetic variability between populations of the ringneck snake Diadophis punctatus species complex. Polymorphism was investigated for these markers in 34 individuals from populations in southeastern Louisiana
and southern Florida. The number of alleles ranged from 5 to 29, expected heterozygosity ranged from 0.816 to 0.962 and observed
heterozygosity ranged from 0.452 to 0.889. These new loci provide the tools necessary to examine species boundaries, population
genetics within species and to determine the conservation status of imperiled populations. 相似文献
16.
1. One important goal in conservation biology is to characterise evolutionary lineages within endangered species before management decisions are taken. Here, we assess population differentiation in the freshwater crayfish Austropotamobius pallipes, an endangered species endemic to western Europe and provide valuable information for the conservation of French populations. 2. Analysis of five microsatellite loci in 44 populations revealed very different within population levels of genetic diversity (0.000 < H0 < 0.564). Two groups, corresponding to northern and southern French populations, showed a high degree of genetic differentiation in both allele frequencies and allele sizes. Comparison of these results with previous studies of A. pallipes strongly suggests that the divergence between northern and southern populations could have occurred during the last glaciation period of the Pleistocene from one Atlantic and one Mediterranean refuge. 3. Evidence for genetic admixture between these two lineages was revealed by correspondence analyses in southern populations, probably as the result of artificial translocations. 4. French populations appeared significantly differentiated among the different river drainages and were highly structured within rivers. The impact of population size, population bottlenecks and founder events on the population genetic differentiation are discussed. 5. Based on these results, we propose the designation of two evolutionarily significant units for A. pallipes in France. Our data also support the maintenance of separate demographic management strategies for crayfish inhabiting different river systems. However, genetic analyses will have to be combined with demographic and ecological data for sustainable conservation programmes. 相似文献
17.
Patricia Delgado Daniel Pinero Alvaro Chaos Nidia Pe´rez-Nasser Elena R. Alvarez-Buylla 《American journal of botany》1999,86(5):669-676
Pinus rzedowskii is an endangered pine species from Michoaca´n (central Me´xico), which has been previously reported from only three localities. Classified within the subgenus Strobus, it exhibits intermediate morphological characters between subgenera Strobus and Pinus. We analyzed genetic aspects that could shed light on the evolution and conservation of this species. The genetic structure of nine populations was examined using 14 isozyme loci. Pinus rzedowskii has a relatively high level of genetic variation with 46.8% of the loci assayed being polymorphic, a total of 35 alleles, and a mean heterozygosity per population of 0.219. We calculated Wright's FST statistic to estimate gene flow indirectly and to evaluate whether or not there was genetic structuring among populations. We found a marked differentiation among populations (FST = 0.175) and significant inbreeding (FIS = 0.247). No pattern of isolation by distance was found. We also constructed a dendrogram based on a genetic distance matrix to obtain an overview of the possible historical relationships among populations. Finally, we found a convex relationship between the genetic distance among populations and the number of ancestral lineages, suggesting that demographically this species has not been at risk recently. Although endangered, with small and fragmented populations, P. rzedowskii shows higher levels of genetic variation than other conifer species with larger populations or similar conservation status. 相似文献
18.
DANIEL VERHAEGEN DANIEL OFORI INZA FOFANA MIREILLE POITEL ALEXANDRE VAILLANT 《Molecular ecology resources》2005,5(4):945-947
Teak (Tectona grandis Linn. f) is a hardwood species of worldwide reputation. It occurs naturally in India, Thailand, Myanmar and Laos. Fifteen highly polymorphic microsatellite loci were developed from a genomic library enriched for AG/TC repeats. Primers to amplify these loci were tested on 265 individual trees from different teak populations. An average of 14.7 alleles per locus were detected and expected heterozygosity ranged from 0.223 to 0.879. These loci constitute a powerful tool in investigating the mating system, gene flow, parentage analysis and population dynamics for use in sustainable management of teak forests and for in situ conservation purposes. 相似文献
19.
20.
The genetic structure of eulachon (Thaleichthys pacificus) populations was examined in an analysis of variation of 14 microsatellite loci representing approximately 1900 fish from 9 sites between the Columbia River and Cook Inlet, Alaska. Significant genetic differentiation occurred among the putative populations. The mean FST for all loci was 0.0046, and there was a significant correlation between population genetic differentiation (FST) and geographic distance. Simulated mixed-stock samples comprising populations from different regions suggested that variation at microsatellite loci provided reasonably accurate estimates of stock composition for potential fishery samples. Marine sampling indicated that immature eulachons from different rivers, during the 2 to 3 years of prespawning life in offshore marine waters, do not mix thoroughly. For eulachons captured incidentally in offshore trawl fisheries, there was a clear geographic cline in relative abundance of eulachons from different geographic areas. The sample from northern British Columbia was dominated by northern and central coastal populations of British Columbia, the sample from central British Columbia was composed of eulachons from all regions, and the sample from southern British Columbia was dominated by Columbia River and Fraser River populations. These results have implications for the management of trawl fisheries and conservation of spawning populations in some rivers where abundance is at historically low levels. 相似文献