共查询到20条相似文献,搜索用时 31 毫秒
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Heat and desiccation are the predominant factors affecting inactivation of Bacillus licheniformis and Bacillus thuringiensis spores during simulated composting
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K. Stanford A. Harvey R. Barbieri S. Xu T. Reuter K.K. Amoako L.B. Selinger T.A. McAllister 《Journal of applied microbiology》2016,120(1):90-98
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B. Setlow S. Parish P. Zhang Y.‐Q. Li W.C. Neely P. Setlow 《Journal of applied microbiology》2014,116(4):805-814
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Lineage‐specific plasmid acquisition and the evolution of specialized pathogens in Bacillus thuringiensis and the Bacillus cereus group
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Guillaume Méric Leonardos Mageiros Ben Pascoe Dan J. Woodcock Evangelos Mourkas Sarah Lamble Rory Bowden Keith A. Jolley Ben Raymond Samuel K. Sheppard 《Molecular ecology》2018,27(7):1524-1540
Bacterial plasmids can vary from small selfish genetic elements to large autonomous replicons that constitute a significant proportion of total cellular DNA. By conferring novel function to the cell, plasmids may facilitate evolution but their mobility may be opposed by co‐evolutionary relationships with chromosomes or encouraged via the infectious sharing of genes encoding public goods. Here, we explore these hypotheses through large‐scale examination of the association between plasmids and chromosomal DNA in the phenotypically diverse Bacillus cereus group. This complex group is rich in plasmids, many of which encode essential virulence factors (Cry toxins) that are known public goods. We characterized population genomic structure, gene content and plasmid distribution to investigate the role of mobile elements in diversification. We analysed coding sequence within the core and accessory genome of 190 B. cereus group isolates, including 23 novel sequences and genes from 410 reference plasmid genomes. While cry genes were widely distributed, those with invertebrate toxicity were predominantly associated with one sequence cluster (clade 2) and phenotypically defined Bacillus thuringiensis. Cry toxin plasmids in clade 2 showed evidence of recent horizontal transfer and variable gene content, a pattern of plasmid segregation consistent with transfer during infectious cooperation. Nevertheless, comparison between clades suggests that co‐evolutionary interactions may drive association between plasmids and chromosomes and limit wider transfer of key virulence traits. Proliferation of successful plasmid and chromosome combinations is a feature of specialized pathogens with characteristic niches (Bacillus anthracis, B. thuringiensis) and has occurred multiple times in the B. cereus group. 相似文献
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Lili Shen Fenglong Wang Yanhua Liu Yumei Qian Jinguang Yang Huiqing Sun 《Journal of Phytopathology》2013,161(4):293-294
Suppression of Tobacco Mosaic Virus (TMV) by B. amyloliquefaciens Ba33 was evaluated on Nicotiana tabacum by spraying before (①), after (②) and simultaneously with (③) TMV inocula. The results suggested that Ba33 treatments reduced local necrotic lesion number and disease index, showing ③ treatment was the best and ① treatment was better than ② treatment in TMV suppression. It also showed Ba33 virus‐contaminated scissors could be disinfected by dipping. Field trials showed that Ba33 had an inhibitory effect of 48.59% in 2009 and 50.54% in 2010, close to the effect of Ningnanmycin, a registered antiviral agent in tobacco. In conclusion, Ba33 might be used as a soil disinfector and an antiviral agent against TMV. 相似文献
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Shigenobu Yoshida Motoo Koitabashi Dai Yaginuma Masato Anzai Minoru Fukuda 《Journal of Phytopathology》2019,167(11-12):679-685
The potential of the active ingredient of a commercial bioinsecticide, XenTari® (Bacillus thuringiensis [BT] serovar aizawai strain ABTS‐1857), to suppress gray mold in tomato plants was elucidated. First, a suspension of the active ingredient of XenTari® and a liquid culture of the bacterial strain as BT inocula were sprayed onto detached leaves or drenched into pots of tomato seedlings, and then, propagules of the gray mold fungus, Botrytis cinerea, were inoculated onto the leaves. The gray mold disease was significantly suppressed when rhizospheres were drenched with either inoculum, but not when inocula were sprayed onto detached leaves of seedlings. Both BT inocula were verified not to directly inhibit the mycelial growth of B. cinerea based on in vitro culture plate assays. Additionally, real‐time RT‐PCR analysis verified that the active ingredient increased the expression levels of defence‐related genes, such as PR‐1(P6) and P4, in the leaves of tomato seedlings. These results suggest that the active ingredient has the potential to suppress gray mold disease in tomato, not through direct antagonistic interactions with B. cinerea, but rather through systemic activation of the plant defence system by increased expression of several defence‐related genes. 相似文献
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Microbial larvicides for mosquito control: Impact of long lasting formulations of Bacillus thuringiensis var. israelensis and Bacillus sphaericus on non‐target organisms in western Kenya highlands
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Yahya A. Derua Samuel C. Kahindi Franklin W. Mosha Eliningaya J. Kweka Harrysone E. Atieli Xiaoming Wang Guofa Zhou Ming‐Chieh Lee Andrew K. Githeko Guiyun Yan 《Ecology and evolution》2018,8(15):7563-7573
The microbial larvicides Bacillus thuringiensis var. israelensis and Bacillus sphaericus have been used extensively for mosquito control and have been found to be effective and safe to non‐target organisms cohabiting with mosquito larvae. Recently developed long lasting microbial larvicides (LLML), although evading the previous challenge of short duration of activity, increase the risk of persistence of toxins in the treated larval habitats. This study monitored the impact of LLML FourStar® and LL3 on non‐target organisms cohabiting with mosquito larvae in an operational study to control malaria vectors in western Kenya highlands. A total of 300 larval habitats were selected in three highland villages. The habitats were first monitored for 5 weeks to collect baseline data on non‐target organisms cohabiting with mosquito larvae and then randomized into two treatment arms (respective FourStar® and LL3) and one control arm. Non‐target organisms were sampled weekly for 5 months after treatment to assess the impact of LLML intervention. Before treatment, the mean density of all non‐target organisms combined in the control, LL3 and FourStar® treated habitats was 1.42, 1.39 and 1.49 individuals per habitat per sampling occasion, respectively. Following treatment, this density remained fairly unchanged for 21 weeks at which time it was 1.82, 2.11, and 2.05 for the respective control, LL3 and FourStar® treated habitats. Statistical analysis revealed that LL3 and FourStar® did not significantly alter abundance, richness or diversity of the 11 taxa studied, when comparing the intervention and control larval habitats. However, both FourStar® and LL3 significantly reduced the density of malaria vectors. In conclusion, one round of label rate application of FourStar® or LL3 in natural larval habitats did not alter richness, abundance or diversity of the monitored aquatic non‐target organisms cohabiting with mosquito larvae to an ecologically significant level. 相似文献
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Isolation and Identification of Bacillus amyloliquefaciens IBFCBF‐1 with Potential for Biological Control of Phytophthora Blight and Growth Promotion of Pepper
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Mengjun Zhang Jilie Li Airong Shen Shiyong Tan Zhun Yan Yongting Yu Zhaodong Xue Taimeng Tan Liangbin Zeng 《Journal of Phytopathology》2016,164(11-12):1012-1021
In this study, 76 bacterial strains were isolated from the rhizosphere soil of pepper. Of these, 23 bacterial isolates capable of inhibiting Phytophthora capsici growth were selected. Among the antagonistic bacteria, one strain, IBFCBF‐1 showed the strongest antagonistic activity, and was identified as Bacillus amyloliquefaciens based on the results of 16S rRNA gene sequence analysis, physiological and biochemical testing, and morphological characteristics. When tested with a dual‐culture method and with laboratory greenhouse studies, the strain IBFCBF‐1 was found to be a potential biocontrol agent for controlling the plant pathogen, P. capsici. Moreover, it showed high efficiency and broad‐spectrum antifungal properties in vitro. Under greenhouse conditions, IBFCBF‐1 could significantly promote the growth of pepper seedlings, and was able to solubilize phosphate, and produce indole acetic acid (IAA) and ammonia. This study clearly demonstrated that IBFCBF‐1 is a potential candidate exhibiting phytophthora blight‐suppressive and plant growth‐promoting effects on pepper. 相似文献
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An experimentally supported model of the Bacillus subtilis global transcriptional regulatory network
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Ashley Rose Bate Timothy Chu Alex Greenfield Bentley Shuster Samantha N Barry Matthew Gallitto Brian Liu Thadeous Kacmarczyk Francis Santoriello Jie Chen Christopher DA Rodrigues Tsutomu Sato David Z Rudner Adam Driks Richard Bonneau Patrick Eichenberger 《Molecular systems biology》2015,11(11)