共查询到20条相似文献,搜索用时 31 毫秒
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Winnie W. Pong Jason Walker Todd Wylie Vincent Magrini Jingqin Luo Ryan J. Emnett Jaebok Choi Matthew L. Cooper Malachi Griffith Obi L. Griffith Joshua B. Rubin Gregory N. Fuller David Piwnica-Worms Xi Feng Dolores Hambardzumyan John F. DiPersio Elaine R. Mardis David H. Gutmann 《PloS one》2013,8(10)
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Hong Zhu Jin Qian Wenqiu Wang Quan Yan Ying Xu Yuan Jiang Lei Zhang Fengqing Lu Weiting Hu Xi Zhang Fenghua Wang Xiaodong Sun 《PloS one》2013,8(3)
Background
Apoptosis of photoreceptors plays a critical role in the vision loss caused by retinal detachment (RD). Pharmacologic inhibition of photoreceptor cell death may prevent RD. This study investigated the role of GADD153 that participates in endoplasmic reticulum (ER) stress-mediated apoptosis of photoreceptor cells after RD.Methods
Retinal detachment was created in Wistar rats by subretinal injection of hyaluronic acid. The rats were then randomly divided into four groups: normal control group, RD group, GADD153 RNAi group and vehicle group. RNA interference of GADD153 was performed using short hairpin RNA (shRNA). Expressions of GADD153 mRNA and protein were examined by RT-PCR and Western blotting analysis, respectively. GADD153 protein distribution in the retinal cells was observed using immunofluorescence confocal laser scanning microscopy. Apoptosis of retinal cells was determined by TdT-mediated fluorescein-16-dUTP nick-end labeling (TUNEL) assay.Results
Lentivirus GADD153 shRNA with the most effective silencing effect was chosen for in vivo animal study and was successfully delivered into the retinal tissues. GADD153 mRNA and protein expressions in GADD153 RNAi group were significantly lower than those in the RD group. Silencing of GADD153 by RNAi protected photoreceptors from ER stress-induced apoptosis.Conclusion
ER stress-mediated pathway is involved in photoreceptor cell apoptosis after RD. GADD153 is a key regulatory molecule regulating ER-stress pathways and plays a crucial role in the apoptosis of photoreceptor cells after RD. 相似文献17.
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