共查询到20条相似文献,搜索用时 953 毫秒
1.
M G A Felipe M Vitolo I M Mancilha S S Silva 《Journal of industrial microbiology & biotechnology》1997,18(4):251-254
The bioconversion of xylose to xylitol by Candida guilliermondii FTI 20037 cultivated in sugar cane bagasse hemicellulosic hydrolyzate was influenced by cell inoculum level, age of inoculum
and hydrolyzate concentration. The maximum xylitol productivity (0.75 g L−1 h−1) occurred in tests carried out with hydrolyzate containing 54.5 g L−1 of xylose, using 3.0 g L−1 of a 24-h-old inoculum. Xylitol productivity and cell concentration decreased with hydrolyzate containing 74.2 g L−1 of xylose.
Received 02 February 1996/ Accepted in revised form 15 November 1996 相似文献
2.
Development of a cost-effective glucose-corn steep medium for production of butanol by Clostridium beijerinckii 总被引:1,自引:0,他引:1
M Parekh J Formanek H P Blaschek 《Journal of industrial microbiology & biotechnology》1998,21(4-5):187-191
Corn steep water (CSW) medium (1.6% solids plus 6% glucose) was evaluated for growth and butanol production by Clostridium beijerinckii NCIMB 8052 wild-type and hyper-amylolytic, hyper-butanol-producing mutant strain BA101. CSW alone was not a suitable substrate,
whereas addition of glucose supported growth and butanol production by both strains. In a batch-scale fermentation using an
optimized 6% glucose-1.6% solids CSW medium, C. beijerinckii NCIMB 8052 and strain BA101 produced 10.7 g L−1 and 14.5 g L−1 of butanol, respectively. The total solvents (acetone, butanol, and ethanol) produced by C. beijerinckii NCIMB 8052 and strain BA101 were 14 g L−1 and 20 g L−1, respectively. Initial fermentation in small-scale flasks containing 6% maltodextrin-1.6% solids concentration CSW medium
resulted in 6 g L−1 and 12.6 g L−1 of butanol production by C. beijerinckii NCIMB 8052 and strain BA101, respectively. CSW can serve as an economic source of nitrogen, vitamins, amino acids, minerals,
and other nutrients. Thus, it is feasible to use 6% glucose-1.6% solids CSW medium in place of semi-defined P2 medium.
Received 9 February 1998/ Accepted in revised form 1 September 1998 相似文献
3.
Delta-endotoxin production by a strain of Bacillus thuringiensis subsp kurstakion complex media based on crude gruel and fish meal was investigated. High proteolytic activities were concomitantly produced
with the bioinsecticide. In such complex media, the repressive regulation due to readily consumed carbon sources was partially
overcome. In order to improve substrate assimilation, 0.5 g L−1 sodium chloride and 0.1% Tween-80 were supplemented to the production medium, increasing delta-endotoxin yields when using
gruel concentrations below 59 g L−1. At and beyond 75 g L−1 gruel, delta-endotoxin yields were not affected in the presence of 0.5 g L−1 NaCl and 0.1% Tween-80, but proteolytic activity yields were remarkably reduced. Thus, the use of sodium chloride and Tween-80
allowed reduction of the initial gruel concentration to 42 g L−1 for the production of 3350 mg L−1 delta-endotoxin, while it was only 3800 mg L−1 with 92 g L−1 gruel. Moreover, similar to 0.5 g L−1 NaCl and 0.1% Tween-80, the use of 10 g L−1 sodium acetate significantly improved delta-endotoxin production and also reduced the proteolytic activity to 250 U ml−1.
Received 05 November 1998/ Accepted in revised form 19 August 1999 相似文献
4.
T Roukas 《Journal of industrial microbiology & biotechnology》1999,22(6):617-621
Aureobasidium pullulans P56 was investigated using an adaptation technique and a mixed culture system. The adaptation of A. pullulans and the mixed cultures of A. pullulans and/or Lactobacillus brevisX20, Debaryomyces hansenii 194 and Aspergillus niger did not increase the production of polysaccharide. Enzymic hydrolysis of lactose in deproteinized whey gave a higher polysaccharide
concentration and polysaccharide yield than acidic hydrolysed lactose. Maximum polysaccharide concentration (11.0 ± 0.5 g L−1), biomass dry weight (10.5 ± 0.4 g L−1), polysaccharide yield (47.2 ± 1.8%) and sugar utilization (93.2 ± 2.8%) were achieved using enzyme-hydrolysed whey (pH 6.5)
containing 25 g L−1 lactose and supplemented with K2HPO4 0.5%, L-glutamic acid 1%, olive oil 2.5%, and Tween 80 0.5%. In this case the pullulan content of the crude polysaccharide was 40%.
Received 16 December 1997/ Accepted in revised form 12 March 1999 相似文献
5.
E Parente C Brienza A Ricciardi G Addario 《Journal of industrial microbiology & biotechnology》1997,18(1):62-67
Production of the bacteriocin enterocin 1146 (E1146) by Enterococcus faecium DPC1146 was studied in batch and continuous fermentation. Growth was strongly inhibited by lactic acid. In batch fermentations
maximum E1146 activity (2.8 MBU L−1) was obtained in 9 h with 20 g L−1 glucose. Increase in initial glucose concentration did not lead to a proportional increase in E1146 activity. A simple linear
model was found to be adequate to explain the relationship between specific bacteriocin production rate and specific growth
rate in batch fermentations with initial glucose concentration higher than 20 g L−1. Maximum bacteriocin activity (2.9–3.2 MBU L−1) was obtained in continuous fermentations at dilution rates between 0.12 and 0.17 h−1 and specific bacteriocin production rate increased linearly with dilution rate.
Received 31 July 1996/ Accepted in revised form 01 November 1996 相似文献
6.
I-Y Lee W T Seo G J Kim M K Kim C S Park Y H Park 《Journal of industrial microbiology & biotechnology》1997,18(4):255-259
Maltose and sucrose were efficient carbon sources for the production of curdlan by a strain of Agrobacterium sp. A two-step, fed-batch operation was designed in which biomass was first produced, followed by curdlan production which
was stimulated by nitrogen limitation. There exists an optimal timing for nitrogen limitation for curdlan production in the
two-step, fed-batch operation. Maximum curdlan production (60 g L−1) was obtained from sucrose with a productivity of 0.2 g L−1 h−1 when nitrogen was limited at a cell concentration of 16.0 g L−1. It was also noted that the curdlan yield from sucrose was as high as 0.45 g curdlan g−1 sucrose, and the highest specific production rate was 1.0 g curdlan g−1 cells h−1 right after nitrogen limitation. Of particular importance was the use of molasses as a cheap carbon source to produce curdlan
in the two-step, fed-batch cultivation. As high as 42 g L−1 of curdlan with a yield of 0.35 g curdlan g−1 total sugar was obtained after 120 h of fed-batch cultivation.
Received 20 August 1996/ Accepted in revised form 26 November 1996 相似文献
7.
A two-phase membrane bioreactor was developed to continuously produce enantiopure epoxides using the epoxide hydrolase activity
of Rhodotorula glutinis. An aqueous/organic cascade, hydrophilic, hollow-fiber membrane bioreactor was used: (1) to carry out large-scale resolution
of epoxides, (2) to continuously extract residual enantiopure epoxides from the aqueous phase, and (3) to separate inhibitory
formed diol from the yeast cells contained in the aqueous phase. Dodecane was employed to dissolve-feed epoxide as well as
to extract residual epoxide. 1,2-Epoxyhexane was used as a model substrate. By use of this membrane bioreactor, enantiopure
(S)-1,2-epoxyhexane (>98% enantiomeric excess) was obtained with a volumetric productivity of 3.8 g l−1 h−1. The continuous-production system was operated for 12 days and resulted in 38 g enantiopure (S)-1,2-epoxyhexane.
Received: 14 February 2000 / Received revision: 15 June 2000 / Accepted: 18 June 2000 相似文献
8.
Liu J Zhang Z Liu Z Zhu H Dang H Lu J Cui Z 《Marine biotechnology (New York, N.Y.)》2011,13(5):837-844
The aim of this work was to optimize the fermentation parameters in the shake-flask culture of marine bacterium Wangia sp. C52 to increase cold-adapted amylase production using two statistical experimental methods including Plackett–Burman
design, which was applied to find the key ingredients for the best medium composition, and response surface methodology, which
was used to determine the optimal concentrations of these components. The results showed starch, tryptone, and initial pH
had significant effects on the cold-adapted amylase production. A central composite design was then employed to further optimize
these three factors. The experimental results indicated that the optimized composition of medium was 6.38 g L−1 starch, 33.84 g L−1 tryptone, 3.00 g L−1 yeast extract, 30 g L−1 NaCl, 0.60 g L−1 MgSO4 and 0.56 g L−1 CaCl2. The optimized cultivation conditions for amylase production were pH 7.18, a temperature of 20°C, and a shaking speed of
180 rpm. Under the proposed optimized conditions, the amylase experimental yield (676.63 U mL−1) closely matched the yield (685.60 U mL−1) predicted by the statistical model. The optimization of the medium contributed to tenfold higher amylase production than
that of the control in shake-flask experiments. 相似文献
9.
N Kiran Sree M Sridhar K Suresh I M Banat L Venkateswar Rao 《Journal of industrial microbiology & biotechnology》2000,24(3):222-226
A repeated batch fermentation system was used to produce ethanol using an osmotolerant Saccharomyces cerevisiae (VS3) immobilized in calcium alginate beads. For comparison free cells were also used to produce ethanol by repeated batch fermentation.
Fermentation was carried for six cycles with 125, 250 or 500 beads using 150, 200 or 250 g glucose L−1 at 30°C. The maximum amount of ethanol produced by immobilized VS3 using 150 g L−1 glucose was only 44 g L−1 after 48 h, while the amount of ethanol produced by free cells in the first cycle was 72 g L−1. However in subsequent fed batch cultures more ethanol was produced by immobilized cells compared to free cells. The amount
of ethanol produced by free cells decreased from 72 g L−1 to 25 g L−1 after the fourth cycle, while that of immobilized cells increased from 44 to 72 g L−1. The maximum amount of ethanol produced by immobilized VS3 cells using 150, 200 and 250 g glucose L−1 was 72.5, 93 and 87 g ethanol L−1 at 30°C. Journal of Industrial Microbiology & Biotechnology (2000) 24, 222–226.
Received 16 September 1999/ Accepted in revised form 22 December 1999 相似文献
10.
A Pessoa Jr I M de Mancilha S Sato 《Journal of industrial microbiology & biotechnology》1997,18(6):360-363
Sugar cane bagasse hemicellulosic fraction submitted to hydrolytic treatment with 100 mg of sulfuric acid per gram of dry
mass, at 140°C for 20 min, was employed as a substrate for microbial protein production. Among the 22 species of microorganisms
evaluated, Candida tropicalis IZ 1824 showed TRS consumption rate of 89.8%, net cell mass of 11.8 g L−1 and yield coefficient (Yx/s) of 0.50 g g−1. The hydrolyzate supplemented with rice bran (20.0 g L−1), P2O5 (2.0 g L−1) and urea (2.0 g L−1) provided a TRS consumption rate of 86.3% and a cell mass of 8.4 g L−1. At pH 4.0 cellular metabolism was inhibited, whereas at pH 6.0 the highest yield was obtained. The presence of furfural
(2.0 g L−1) hydroxymethylfurfural (0.08 g L−1) and acetic acid (3.7 g L−1) in the hydrolyzate did not interfere with cultivation at pH 6.0.
Received 25 October 1996/ Accepted in revised form 10 March 1997 相似文献
11.
Cloning and characterization of an epoxide hydrolase-encoding gene from Rhodotorula glutinis 总被引:1,自引:0,他引:1
Visser H Vreugdenhil S de Bont JA Verdoes JC 《Applied microbiology and biotechnology》2000,53(4):415-419
We cloned and characterized the epoxide hydrolase gene, EPH1, from Rhodotorula glutinis. The EPH1 open reading frame of 1230 bp was interrupted by nine introns and encoded a polypeptide of 409 amino acids with a calculated
molecular mass of 46.3 kDa. The amino acid sequence was similar to that of microsomal epoxide hydrolase, which suggests that
the epoxide hydrolase of R. glutinis also belongs to the α/β hydrolase fold family. EPH1 cDNA was expressed in Escherichia coli and resting cells showed a specific activity of 200 nmol min−1 (mg protein)−1 towards 1,2-epoxyhexane.
Received: 2 August 1999 / Received revision: 4 October 1999 / Accepted: 10 October 1999 相似文献
12.
P Buzzini 《Journal of industrial microbiology & biotechnology》2000,24(1):41-45
A multivariate statistical approach was employed for the optimization of conditions for carotenoid production by Rhodotorula glutinis DBVPG 3853 from a substrate containing concentrated rectified grape must as the sole carbohydrate source. Several experimental
parameters (carbohydrate, yeast autolysate and salt concentrations, and pH) were tested at two levels by following a fractional
factorial design. Carotenogenesis was most sensitive to both initial pH and yeast autolysate concentration. A Central Composite
Design experiment was then performed by obtaining both second-order polynomial models and isoresponse diagrams where initial
pH and yeast autolysate concentration were considered as variables. In this way it was possible to determine the conditions
(pH = 5.78, yeast autolysate = 4.67 g L−1) which maximize both the concentration of total carotenoids and that of β-carotene (6.9 mg L−1 and 1100 μg L−1 of culture fluid, respectively, after 120 h of fermentation). Journal of Industrial Microbiology & Biotechnology (2000) 24, 41–45.
Received 23 February 1999/ Accepted in revised form 14 September 1999 相似文献
13.
Candida peltata NRRL Y-6888 to ferment xylose to xylitol was evaluated under different fermentation conditions such as pH, temperature, aeration,
substrate concentration and in the presence of glucose, arabinose, ethanol, methanol and organic acids. Maximum xylitol yield
of 0.56 g g−1 xylose was obtained when the yeast was cultivated at pH 6.0, 28°C and 200 rpm on 50 g L−1 xylose. The yeast produced ethanol (0.41 g g−1 in 40 h) from glucose (50 g L−1) and arabitol (0.55 g g−1 in 87 h) from arabinose (50 g L−1). It preferentially utilized glucose > xylose > arabinose from mixed substrates. Glucose (10 g L−1), ethanol (7.5 g L−1) and acetate (5 g L−1) inhibited xylitol production by 61, 84 and 68%, respectively. Arabinose (10 g L−1) had no inhibitory effect on xylitol production.
Received 24 December 1998/ Accepted in revised form 18 March 1999 相似文献
14.
Production of hexanoic acid from d-galactitol by a newly isolated Clostridium sp. BS-1 总被引:1,自引:0,他引:1
Byoung Seung Jeon Byung-Chun Kim Youngsoon Um Byoung-In Sang 《Applied microbiology and biotechnology》2010,88(5):1161-1167
In a study screening anaerobic microbes utilizing d-galactitol as a fermentable carbon source, four bacterial strains were isolated from an enrichment culture producing H2, ethanol, butanol, acetic acid, butyric acid, and hexanoic acid. Among these isolates, strain BS-1 produced hexanoic acid
as a major metabolic product of anaerobic fermentation with d-galactitol. Strain BS-1 belonged to the genus Clostridium based on phylogenetic analysis using 16S rRNA gene sequences, and the most closely related strain was Clostridium sporosphaeroides DSM 1294T, with 94.4% 16S rRNA gene similarity. In batch cultures, Clostridium sp. BS-1 produced 550 ± 31 mL L−1 of H2, 0.36 ± 0.01 g L−1 of acetic acid, 0.44 ± 0.01 g L−1 of butyric acid, and 0.98 ± 0.03 g L−1 of hexanoic acid in a 4-day cultivation. The production of hexanoic acid increased to 1.22 and 1.73 g L−1 with the addition of 1.5 g L−1 of sodium acetate and 100 mM 2-(N-morpholino)ethanesulfonic acid (MES), respectively. Especially when 1.5 g L−1 of sodium acetate and 100 mM MES were added simultaneously, the production of hexanoic acid increased up to 2.99 g L−1. Without adding sodium acetate, 2.75 g L−1 of hexanoic acid production from d-galactitol was achieved using a coculture of Clostridium sp. BS-1 and one of the isolates, Clostridium sp. BS-7, in the presence of 100 mM MES. In addition, volatile fatty acid (VFA) production by Clostridium sp. BS-1 from d-galactitol and d-glucose was enhanced when a more reduced culture redox potential (CRP) was applied via addition of Na2S·9H2O. 相似文献
15.
Survase SA Jurgens G van Heiningen A Granström T 《Applied microbiology and biotechnology》2011,91(5):1305-1313
Clostridium beijerinckii DSM 6423 was studied using different continuous production methods to give maximum and stable production of isopropanol and
n-butanol. In a single-stage continuous culture, when wood pulp was added as a cell holding material, we could increase the
solvent productivity from 0.47 to 5.52 g L−1 h−1 with the yield of 54% from glucose. The overall solvent concentration of 7.51 g L−1 (39.4% isopropanol and 60.6% n-butanol) with the maximum solvent productivity of 0.84 g L−1 h−1 was obtained with two-stage continuous culture. We were able to run the process for more than 48 overall retention times
without losing the ability to produce solvents. 相似文献
16.
The maximum ethanol concentration produced from glucose in defined media at 45°C by the thermotolerant yeast Kluyveromyces marxianus IMB3 was 44 g L−1. Acclimatisation of the strain through continuous culture at ethanol concentrations up to 80 g L−1, shifted the maximum ethanol concentration at which growth was observed from 40 g L−1 to 70 g L−1. Four isolates were selected from the continuous culture, only one of which produced a significant increase in final ethanol
concentration (50 ± 0.4 g L−1), however in subsequent fermentations, following storage on nutrient agar plates, the maximum ethanol concentration was comparable
with the original isolate. The maximum specific ethanol production rates (approximately 1.5 g (gh)−1) were also comparable with the original strain except for one isolate (0.7 g (gh)−1). The specific ethanol productivity decreased with ethanol concentration; this decrease correlated linearly (rval 0.92) with
cell viability. Due to the transience of induced ethanol tolerance in the strain it was concluded that this was not a valid
method for improving final ethanol concentrations or production rates.
Received 18 July 1997/ Accepted in revised form 19 February 1998 相似文献
17.
Jonny E. Scherwinski-Pereira Rodrigo S. da Guedes Paulo César P. FerminoJr Tatiane L. Silva Frederico Henrique S. Costa 《In vitro cellular & developmental biology. Plant》2010,46(4):378-385
An efficient procedure has been developed for inducing somatic embryogenesis and regeneration of plants from tissue cultures
of oil palm (Elaeis guineensis Jacq.). Thin transverse sections (thin cell layer explants) of different position in the shoot apex and leaf sheath of oil
palm were cultivated in Murashige and Skoog (MS) (Physiol Plant 15:473–497, 1962) medium supplemented with 0–450 μM picloram and 2,4-D with 3.0% sucrose, 500 mg L−1 glutamine, and 0.3 g L−1 activated charcoal and gelled with 2.5 g L−1 Phytagel. Embryogenic calluses were evaluated 12 wk after inoculation. Picloram (450 μM) was effective in inducing embryogenic
calluses in 41.5% of the basal explants. Embryogenic calluses were maintained on a maturation medium composed of basal media,
plus 0.6 μM NAA and 12.30 μM 2iP, 0.3 g L−1 activated charcoal, and 500 mg L−1 glutamine, with subcultures at 4-wk intervals. Somatic embryos were converted to plants on MS medium with macro- and micronutrients
at half-strength, 2% sucrose, and 1.0 g L−1 activated charcoal and gelled with 2.5 g L−1 Phytagel. 相似文献
18.
Jeffke T Jende D Mätje C Ehlers RU Berthe-Corti L 《Applied microbiology and biotechnology》2000,54(3):326-330
Photorhabdus luminescens, a bacterial symbiont of entomopathogenic biocontrol nematodes, was grown in batch and glucose fed-batch culture. The cell
density, bioluminescence, production of antibiotic substances, number of cells with inclusion bodies, glucose concentration
and oxygen uptake rate were recorded. The addition of 12.4 g l−1 glucose prolonged the growth, and the yield almost doubled, from 6.85 g l−1 to 12.45 g l−1 dry mass. The production of antibiotic substances increased by 140%. Bioluminescence was higher in the batch culture. A shift
of P. luminescens to phase II variants was not detected.
Received: 21 January 2000 / Received revision: 3 April 2000 / Accepted: 7 April 2000 相似文献
19.
L. Lesage-Meessen M. Haon M. Delattre J.-F. Thibault B. Colonna Ceccaldi M. Asther 《Applied microbiology and biotechnology》1997,47(4):393-397
The effects of adding cellobiose on the transformation of vanillic acid to vanillin by two strains of Pycnoporus cinnabarinus MUCL39532 and MUCL38467 were studied. When maltose was used as the carbon source in the culture medium, very high levels
of methoxyhydroquinone were formed from vanillic acid. When cellobiose was used as the carbon source and/or added to the culture
medium of P. cinnabarinus strains on day 3 just before vanillic acid was added, it channelled the vanillic acid metabolism via the reductive route
leading to vanillin. Adding 3.5 g l−1 cellobiose to 3-day-old maltose cultures of P. cinnabarinus MUCL39532 and 2.5 g l−1 cellobiose to 3-day-old cellobiose cultures of P. cinnabarinus MUCL38467, yielded 510 mg l−1 and 560 mg l−1 vanillin with a molar yield of 50.2 % and 51.7 % respectively. Cellobiose may either have acted as an easily metabolizable
carbon source, required for the reductive pathway to occur, or as an inducer of cellobiose:quinone oxidoreductase, which is
known to inhibit vanillic acid decarboxylation.
Received: 24 July 1996 / Received revision: 29 November 1996 / Accepted: 29 November 1996 相似文献
20.
E. J. Bormann M. Leißner M. Roth B. Beer K. Metzner 《Applied microbiology and biotechnology》1998,50(5):604-607
Polyhydroxybutyrate (PHB) was produced by Ralstonia eutropha DSM 11348 (formerly Alicaligenes eutrophus) in media containing 20–30 g l−1 casein peptone or casamino acids as sole sources of nitrogen. In fermentations using media based on casein peptone, permanent
growth up to a cell dry mass of 65 g l−1 was observed. PHB accumulated in cells up to 60%–80% of dry weight. The lowest yields were found in media without any trace
elements or with casamino acids added only. The residual cell dry masses were limited to 10–15 g l−1 and did not contain PHB. The highest productivity amounted to 1.2 g PHB l−1 h−1. The mean molecular mass of the biopolymer was determined as 750 kDa. The proportion of polyhydroxyvalerate was less than
0.2% in PHB. The bioprocess was scaled up to a 300-l plant. During a fermentation time of 39 h the cells accumulated PHB to
78% w/w. The productivity was 0.98 g PHB l−1 h1.
Received: 8 July 1998 / Accepted: 26 August 1998 相似文献