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1.
The reacting pattern of circulating filarial antigen fraction-2 fromWuchereria bancrofti and soluble antigen from adultBrugia malayi with bancroftian filarial sera were analysed by immunoblotting technique and enzyme linked immunosorbent assay. Microfilaraemic
sera reacted specifically with proteins of molecular weight 200, 120, 97, 56, 54, 43, 26 and 17 kDa of circulating Filarial
antigen fraction-2 and 44, 40, 38, 31, 22 and 18 kDa ofBrugia malayi adult soluble antigen. Clinical filarial sera identified protein molecules of 56, 54 and 42 kDa of circulating filarial antigen
fraction-2 and 19, 16 and 14 kDa ofBrugia malayi adult soluble antigen. Some components of both the antigen preparation were also identified by endemic normal serai.e.proteins 120, 97, 62, 43 and 33 kDa of circulating filarial antigen fraction-2 and 170, 120, 43, 31 and 12 kDa ofBrugia malayi adult soluble antigen. One of the sodium dodecyl sulphate-polyacrylamide gel electropherosis fractions of circulating filarial
antigen fraction-2 (CFA2-8) andBrugia malayi adult soluble antigen fraction-6 when used in enzyme linked immunosorbent assay could differentiate microfilaraemic sera
from endemic normal and clinical filarial sera. The other antigen fractions (CFA2-2, 6 and 7 andBmA-2) showed a high geometric mean titre of filarial immunoglobulin G antibodies in endemic normal sera when compared to microfilaraemia
and clinical filarial sera. These proteins need to be further studied to assess their involvement in protecting from filarial
infection in an endemic area. 相似文献
2.
The cell wall protein peptidoglycan complex (CW-PPC) of
Mycobacterium tuberculosis H37Ra was isolated through sequential extraction of lipids, carbohydrates and soluble proteins. CW-PPC emulsified in FIA was
found to induce significant protection in mice against challenge with LD50 dose ofM. tuberculosis H37Rv. To identify the immunoprotective components of CW-PPC, the proteins in avid association with peptidogican were dissociated
by chemical treatment with trifluoromethanesulthonic acid (CF3CO3H): anisole (2:1). Immunoreactivity of total (CW-Pr) as well as its component proteins i.e., 71, 60 and 45 kDa proteins of
cell wall was studied in animals immunized with CW-Pr-FIA. The 71 kDa protein was found to be most immunoreactive giving higher
T-cell sensitization and humoral responses. Further, immunization of mice with 71 kDa-FIA demonstrated enhanced T- and B-
cell responses. Mice immunized with 71 kDa-FIA gave significantly higher protection (P ≤ 0.05) against intravenous challenge
with LD50 dose ofM. tuberculosis H37Rv, than BCG immunized animals. The results indicate the potential of 71 kDa cell wall protein as a suitable candidate for
Cthe subunit vaccine. 相似文献
3.
Streptomyces fradiae
produces several extracellular proteases and many of these are inducible. An 8.8 kb DNA fragment of
Streptomyces fradiae
cloned on pIJ699 caused increased protease activity in
Streptomyces lividans.Clones carrying this recombinant plasmid showed a significant delay in sporulation. A protein of 18 kDa was purified from
the extracellular proteins secreted by the host carrying the recombinant plasmid. Further characterization showed that this
protease is a metalloprotease. 相似文献
4.
Tony M. Johnson A. S. Rishi Pritilata Nayak Soumitra K. Sen 《Journal of biosciences》1996,21(5):673-685
Damage caused to rice production by coleopteran insects like rice weevil (Sitophilus oryzae), a stored grain insect pest and rice hispa (Dicladispa armigera), a pest of the growing plant is quite high. In order to combat the damage, generation of insect resistant transgenic rice
plant was considered desirable. CryIIIA endotoxin ofBacillus thuringiensis var.tenebrionis, a 65 kDa protein toxic to coleopteran insects, figured as the candidate gene product. Thus, the cryIIIA gene was isolated
from a local isolate ofBacillus thuringiensis var.tenebrionis. The gene was tailored at the N-terminal end to its minimal size by using a synthetic ATG codon which replaced the first
codon next to ATG of threonine to proline. This modification did not affect the functional property of the gene product. A
chimeric construct of the modifiedcryIIIA gene was developed containing CaMV35S promoter andnos terminator for plant expression. The expressibility of thecryIIIA gene inindica rice was judged through test for transient expression in indica rice protoplasts. 相似文献
5.
Om Parkash M. K. Beuria B. K. Girdhar K. Katoch U. Sengupta 《Journal of biosciences》1997,22(1):111-116
Skin scrapings obtained from the lesions of leprosy patients of all types showed 96 % positivity to the serum antibody competition
test using monoclonal antibody (ML04)to 35 kDa antigen of
Mycobacterium leprae. Further, in vitro culture of full thickness skin biopsies from lepromatous patients were noted to release IgG antibodies
toM. leprae with a peak antibody response at 48 h. The significance of this local antibody response toM. leprae in skin has been discussed for its possible use in diagnosing early leprosy. 相似文献
6.
In an attempt to develop measures for early diagnosis and prognosis of the disease and to explore association of murine mammary
tumour virus (MuMTV) or related virus in breast cancer, we purified a breast tumour associated antigen (BTAA) from the breast
tumour tissues of untreated female cancer patients. The BTAA purified by DEAE discontinuous column chromatography followed
by SE-HPLC was an 85 kDa glycoprotein. A high level of circulating antibodies against this antigen was observed, using ELISA,
in all the untreated female breast cancer patients. The BTAA was not immunologically related to MuMTV antigens but strongly
resembled an 83 kDa glycoprotein tumour associated antigen, purified from MuMTV induced mouse mammary tumour. In patients
after surgical removal of the breast tumour, the circulating antibodies to the BTAA decreased gradually, but reappeared in
the patients with secondary metastasis. In healthy age matched women or in female patients with carcinoma of tissues other
than breast, no significant titre of the BTAA antibodies was observed. 相似文献
7.
The 47–55 domain of the maturehumanInterleukin-was predicted to be exposed by our computational analysis and confirmed to
be so by comparing with X-ray crystallographic as well as nuclear magnetic resonance (NMR) spectroscopic data. Four peptides
representing fully or part of this domain with sequences 47–55, 41–61, 45–61 and 50–66 were synthesized and tested for their
ability to modulate in vivo, the humoral immune response of Balb/c mice to Shigella dysenteriae 116 kDa antigen(s). The smallest
immunomodulatory peptide amongst them was found to be the nonapeptide 47–55. To ascertain the structure-function relationships
of this 47–55 peptide, various mutant peptides were synthesized and tested for IL-1β
2 like activity in vivo. Change of Val47 to Asp47 or to Lys47 enhanced its immunomodulatory activity significantly while the change of Gly49 to Asp49 or Glu50 to Ile50 or Asp54 to Ile54 had no such effect. The peptides 47–55 and its mutants were first tested for their ability to elicit inflammatory response
like PGE2 synthesis by a sensitive radioimmunoassay. The peptides which did not have any inflammatory activity were then tested for
their ability to stimulate antigen primed T-cells in vitro in the presence of sub-optimal concentration of the antigen. 相似文献
8.
S. Mohan Jain 《Journal of biosciences》1997,22(5):585-592
Begonia
x
elatior
plantlets which regenerated from leaf disk callus showed variations in plant morphology, number of flowers per plant, and
flower size. Variations in flowering period, number of flowers per plant, and flower morphology were observed in
Saintpaulia ionantha
L. plants directly regenerated from leaf disk explants. The cytokinins, benzylaminopurine and zeatin, tested in the culture
medium did not affect the basic plant characteristics including flower colour which remained stable in both species. Micropropagation
of selected somaclones having the desirable trait of high number of flowers per plant was stable in the MV2 and MV3 generations. 相似文献
9.
Gábor Nardai Tamás Schnaider Csaba Söti Michael T. Ryan Peter B. Hoj János Somogyi Peter Csermely 《Journal of biosciences》1996,21(2):179-190
The 90 kDa heat shock protein (HSP90) is an ATP-binding molecular chaperone with an associated ATPase activity having nucleoplasmin
and HSP70-binding homology domains and containing Ca-binding EF-hands and a nuclear localization signal. Here we characterize
the HSP90-associated ATPase and show that it is (i) a P-type ATPase inhibited by molybdate and vanadate, (ii) able to hydrolyze
methylfluorescein phosphate with a 5–6-fold higher affinity, (iii) a 3-times better GTPase than ATPase in the presence of
calcium and (iv) HSP27 and F-actin, but not HSP10 can “convert” the HSP90-associated ATPase activity to HSP90 autokinase activity.
The HSP90-associated ATP/GTPase may participate in the regulation of complex formation of HSP90 with other proteins, such
as F-actin, tubulin and heat shock proteins. 相似文献
10.
Blackgram (Vigna mungo
L. Hepper)seeds contain two galactose-specific lectins, BGL-I and BGL-II. BGL-I was partially purified into two monomeric lectins which
were designated as BGL-I-1 (94 kDa) and BGL-I-2 (89 kDa). BGL-II is a monomeric lectin of 83 kDA. The purified lectins were
associated with galactosidase activities. BGL-I-1 and BGL-II were copurified with α-galactosidase activity while BGL-I-2 was
largely associated with β-galactosidase activity. These lectins agglutinate trypsin treated rabbit erythrocytes, but not the
human erythrocytes of A, B or O groups. They were stable between pH 3·5 and 7·5 for their agglutination. The lectins did not
show any metalion requirement. They were inactivated at 50°C. The lectin activity was inhibited by D-galactose (0·1 mM). The
Scatchard plots of galactose binding to these lectins are nonlinear and biphasic curves indicative of multiple binding sites.
The data show that the monomeric lectins have both lectin and galactosidase activities suggestive of a bifunctional protein. 相似文献
11.
Grewal J.S. Reddy M.P. Singh Arvinderjit Singh Mohinder 《World journal of microbiology & biotechnology》2002,18(8):721-725
Secretion of a low molecular weight (24 kDa) Mycobacterium tuberculosis-specific antigen was analysed in the urine samples of tuberculosis patients under antimycobacterial therapy regime. The urine samples of sputum-positive and culture-positive tuberculosis patients under therapy regime were collected after 2, 3, 4, 5 and 6 months of antimycobacterial therapy. After concentration of the samples by ultrafiltration the proteins were resolved by SDS–PAGE. The antibodies raised in rabbits against M. tuberculosis strain H37Ra were used to check specificity of the bands by Western blotting. It was found that the tuberculosis patients secreted a 24 kDa M. tuberculosis-specific antigen in their urine. This band was present in the samples taken upto 5 months of drug therapy but was absent in the samples taken after 6 months of antimycobacterial therapy. This study gives evidence in support of the continuation of chemotherapy of tuberculosis patients for at least 6 months. Also, the 24 kDa excreted/secreted antigen can be used as a marker for monitoring the drug therapy as well as for the diagnosis of tuberculosis patients. Moreover, the urine sample taken in the study is a non-invasive and safer sampling method as compared to sampling blood or sputum which is the usual procedure in these patients. 相似文献
12.
Nilanjana Basu Labanyamoy Kole Abhijit Ghosh Pijush K Das 《Molecular and cellular biochemistry》1994,132(1):1-6
A rabbit polyclonal antibody to crude soluble antigen ofLeishmania donovani promastigotes recognized a determinant expressed on the surface membrane of mouse peritoneal macrophages and human monocyte derived macrophages infectedin vitro. The determinant was recognized on infected macrophage surface only when F (ab)2 fragments of anti-leishmanial antiserum was employed in immunofluorescence. F(ab)2 fragments of human patient sera also could recognize the determinant. The expression of this antigen was not stage-specific for the parasite. Immunochemical analyses revealed this antigen to be of 51 kDa protein. Specific leaching of membrane proteins by trypsin showed three bands of expressed antigens of 26, 11 and 10 kDa, which in all likelihood might be arising from the 51 kDa antigen. The antigen was not expressed until 12 h of post infection, reached a maximum level at 24 h and thereafter attained a steady state level as studied upto 96 h of post infection. This typc of antigen might have a great potential in immunodiagnostics and site-specific drug targeting. 相似文献
13.
Seiji Kawamoto Masahiro Shoji Yuko Setoguchi Masatoshi Kato Shuichi Hashizume Akira Ichikawa Kazuhiro Osada Yoshinori Katakura Hirofumi Tachibana Hiroki Murakami 《Cytotechnology》1995,17(2):103-108
The human monoclonal antibody AE6F4 specifically reacts with human lung cancer tissues but does not with normal tissues. This monoclonal antibody recognizes a cytosolic 31 kDa antigen in the cancer cells. In a previous study, we elucidated that the 31 kDa antigen belonged to a family of proteins collectively designated as 14-3-3 proteins, which were known as protein kinase-dependent activators of tyrosine/trytophan hydroxylases, or protein kinase C inhibitor proteins. Here we report molecular cloning of the 31 kDa antigen from the human lung adenocarcinoma cell line, A549. Sequencing analysis indicates that the cloned cDNA is identical to that of previously reported human placental cytosolic phospholipase A2 (cPLA2), which is also a member of the 14-3-3 protein family. Western analysis demonstrated that a 31 kDa recombinant cPLA2 expressed in monkey COS cells was recognized by the AE6F4 monoclonal antibody. Binding of the monoclonal antibody to the recombinant cPLA2 was abolished when treated with sodium periodate, suggesting that not only are carbohydrate chains associated with the cPLA2, but they also play a crucial role in antigen recognition by the monoclonal antibody. 相似文献
14.
The integration, expression, and stability of the Respiratory Syncytial Virus (RSV)-F protein was analyzed in a T3 generation of transgenic cherry tomato, Solanum
lycopersicum L. cv. Swifty Belle, plants. Expression of the RSV-F antigen, under the control of the fruit-specific promoter E-8, was investigated
in T3 plants derived from a transgenic line, identified as #120. Transgene integration of the RSV-F gene in the T3 generation was initially determined by polymerase chain reaction (PCR). PCR analysis from line 120-7-2 revealed that all
T3 plants were homozygous for the transgene; whereas, line 120-6-4 showed segregation for the transgene. Enzyme-linked immunosorbent
assay (ELISA) was used to quantify levels of RSV-F protein in these plants, and protein levels ranged from 0–22 μg/g of fresh
weight, with an average of ~3 μg/g fresh weight. Southern blot analysis of the highest expressing plants revealed presence
of a single copy of the RSV-F transgene in these plants. 相似文献
15.
Thehrsω or the 93D heat shock locus ofDrosophila melanogaster, which does not code for any protein, has an important role in development since nullosomy of this locus in transheterozygotes
for two overlapping deficiencies, viz.,Df(3R)
e
Gp4 (eGp4) andDf(3R)GC14 (GC14), is known to cause a high (∼ 80%) mortality with the small number of escapee nullosomic flies being sterile, weak and surviving
for only a few days. We now show that a majority of thehsrω-nulosomics die as embryo and that the 20% escapee embryos develop slower compared to their sibs carrying either one or two
copies of thehsrω locus but after hatching survive to pupal/imago stage. Most interestingly, we further show that when onehsp83 mutant allele (hsp83
e4A) is introduced ineGp4/GC14 trans-heterozygotes, practically none of thehsrω-nullosomic embryos develop beyond the 1st instar larval stage. The specificity of this interaction betweenhsp83 andhsrω genes was further confirmed by examining the effect of thehsp83 mutant allele on other mutations in the 93D cytogenetic region. Therefore, we conclude that thehsp83 mutation acts as a dominant enhancer of the lethality associated with nullosomy for thehsrω gene. The observed genetic interaction between these two members of the heat shock gene family during normal embryonic development
ofDrosophila reveals novel aspects of their biological functions. 相似文献
16.
This paper presents the results of an investigation on the distribution of210Po in Mutharasanallur pond ecosystem. It has been demonstrated that210Po is non-uniformly distributed within the ecosystem. The results of the study show a dissolved210Po concentration in pond water of 1 4mBq 1−1. The sediment samplso recorded a210Po activity of 59 9 Bq kg−1. The aquatic organisms showed differential accumulation of the radionuclide with enhanced bioaccumulation in soft tissues
and muscle. The210Po activity in the biota fell within the range of 1·2–53 3 Bq kg−1 (wet wt). The bivalve mussel,Lamellidens marginalis was identified to accumulate higher concentration of210Poin soft tissues, suggesting that these organisms could serve as a bio-monitor of210Po radionuclide in a freshwater system. The concentration factors of210Po for the biotic components ranged from ∼102–∼104. Analyses of the results indicate that prawn and fish represent an important source of supply of210Po to humans via dietary intake. Results of210Po activity in the abiotic and biotic components of the pond ecosystem were higher when compared with those of Cauvery river
system, the primary water source of the pond. 相似文献
17.
S. A. Ranade Anil Kumar Mamta Goswami Nuzhat Farooqui P. V. Sane 《Journal of biosciences》1997,22(4):457-464
Amaranths are an important group of plants and include grain, vegetable and ornamental types. Despite the economic importance
of the amaranths, there is very little information available about the extent and nature of genetic diversity present in the
genus
Amaranthus
at molecular level. We now report the randomly amplified polymorphic DNA (RAPD) profiles of different species of
Amaranthus
as well as different accessions of the species. These RAPD analyses have been carried out using 65 arbitrary sequence decamer
primers. From the RAPD data, an UPGMA dendrogram illustrating the inter-as well as intra-species relationships has been computed.
The putative hybrid origin of A.dubious
from
A. hybridus
and
A. spinosus
is also ruled out by the RAPD data. The trends of species relationships amongst the amaranths determined by RAPDs is consistent
with their cytogenetic and evolutionary relationships that have already been determined.
NBRI Communication No:464 (N.S.). 相似文献
18.
Wheamei Jenq Chi Lan Chen Chun Chang Chang Richard F. E. Crang 《Archives of microbiology》1994,162(1-2):33-40
Selected strains of Candida albicans were examined to reveal the surface antigenicity and biochemical nature of major cell wall proteins that also were shown to serve as cellular adhesins on human buccal epithelial cells. Confirmation of the adhesive properties of these cells was made by scanning electron microscopy and immunofluorescence microscopy. Particular attention was directed at the clinical isolate KM-302. By means of indirect immunofluorescence staining, the KM-302 blastoconidia absorbed rabbit anti-C. albicans ATCC-32354 serum, revealing specific localization of surface antigens on germ tubes and pseudohyphae. Extracellular polymeric material and the cell wall extract of C. albicans KM-302 blastoconidia were found to contain a major surface antigen of 49 kDa that exhibited 42% adhesion inhibition in vitro. Of considerable significance is that immunogold localization by electron microscopy showed the antigen to be almost exclusively cell wall bound. This major antigen, identified in affinity and gel filtration chromatography fractions, was composed of 4% carbohydrate and 95.7% protein and had an isoelectric point of 6.1. The major antigen also showed a high level of similarity with that of C. albicans strain SC-5314 inasmuch as the major antigen of that strain had carbohydrate and protein compositions of 4 and 95.5%, respectively. Both of these strains also possessed the same percent of adhesion inhibition of human buccal epithelial cells.Abbreviations
BECs
buccal epithelial cells
-
CWE
cell wall extract
-
EPP
extracellular polymers and proteins
-
FITC
fluorescein isothiocyanate
-
mAg
major antigen
-
OD
600
optical density at 600 nm
-
PBS
phosphate buffered saline
-
TEM
transmission electron microscopy
-
YNB
yeast nitrogen base 相似文献
19.
Gastrimargus africanus orientalis,an acridid grasshopper has revealed the existence of karyotypic mosaicism in the male germ line cells of a few individuals
with 2
n
= 23, 19, 21, 25 and 27 chromosomes. Details of this chromosomal instability are presented in this paper.
We dedicate this paper to our teacher Prof. L Siddaveere Gowda on the eve of his 60th birthday. 相似文献
20.
The ultradian rhythm of the lateral leaflets ofDesmodium motorium}(Houtt.) Merril. was recorded with a picture analysis method using a video camera and a computer. The periods are in the minute
range and depend strongly on temperature.
The phosphatidyl inositol signal chain might be involved in the ultradian rhythm of the lateral leaflet movement of
Desmodium motorium:Myoinositol shortens the period length and reduces the known period lengthening effect of lithium ions. Neomycin, which inhibits
the hydrolysis of phosphatidylinositol-4,5 -biphosphate to inositol-4-phosphate and diacylglycerin, lengthens the period of
the rhythm at low concentrations (0.2 mM). Higher concentrations shorten the period, perhaps by activating G protein. Mastoparan,
which activates G protein, shortens period likewise. The G protein agonists fluorid ion and ethanol are toxic for the lateral
leaflets and could therefore not be used to test the involvement of G protein.
The intracellular Ca
2+
antagonist 3,4,5-trinietlioxybeiizoic acid 8-(diethylamino)octylester lengthens the period of the rhythm. This indicates,
that release of Cas
2+
from intracellular stores is important for the lateral leaflet movement rhythm. 相似文献