Rethinking the links between systematic studies and ex situ living collections as a contribution to the Global Strategy for Plant Conservation

The Muséum national d’histoire naturelle (MNHN) in Paris holds ca. 70 million specimens. The collections were in need of a strategy to ensure their long-term conservation. We discuss how the Department of Botanical and Zoological Gardens (DJBZ; tropical living collections), and the Department of Systematics and Evolution (DSE; herbarium) contribute to achieving GSPC’s Target 1 (‘a widely accessible working list of known plant species as a step towards a complete world flora’). The DJBZ started encouraging better management of the collections, evolving towards focused reference collections, where all specimens have well-documented collection data. The objective is to link all collections to a scientific referee. This has already been achieved for a number of taxa. The herbarium of the DSE (acronym P) is among the world’s largest (11 million specimens, including 400,000 types). The collection’s heterogeneity impedes access to its data, since P is a mix of recent well-documented collections and historical collections at various curational levels. P is currently under renovation, which started by mounting all ca. 2 million unmounted specimens. The project also includes databasing and imaging of every specimen. The database now holds around 1,000,000 records. For taxonomic studies, living collections are crucial, especially for plants that are not easily preserved as herbarium specimens. Living collections also enable studies impossible to forecast at the time of collecting. Herbaria and living collections should therefore be conceived as interoperable entities requiring common scientific curation. Through a combination of its assets and the expertise of its researchers, the MNHN is well prepared to tackle the new objectives of the GSPC beyond 2010.     

Using predictive modelling to guide the conservation of a critically endangered coastal wetland amphibian

Amphibians are the most threatened Class of vertebrate, with wetland-associated anurans in particular suffering high levels of habitat loss. We used predictive modelling to better understand the distribution of a critically endangered South African endemic (Hyperolius pickersgilli) and to guide conservation action. MaxEnt distribution models were produced based on limited occurrence data. Predicted localities with probability of occurrence ≥60% were surveyed. Ten new sub-populations were discovered. The mean probability of occurrence for the species at wetlands where it was detected was greater than that at wetlands where it was not detected or absent. In addition, 17 known historical localities were re-visited and the species deemed absent at 8 of these. The total number of localities at which the species is now known to occur is 18, which is an increase in the known extant sub-populations of six. We recalculate the area of occupancy and extent of occurrence for the species as 108 km² and 2081.5 km², respectively; both increases on previous estimates. Implications of these changes on the IUCN Red List status of H. pickersgilli are discussed. A friction map was created to identify possible linkages between sub-populations, which can be used to guide habitat restoration and population repatriation. Given the degree of isolation of subpopulations and the potentially severe threats to most of these, urgent conservation action for H. pickersgilli remains crucial. This study provides a method for use in conservation planning for wetland-breeding amphibians in eastern coastal regions of Africa and elsewhere.     

A Proteomic Strategy for Global Analysis of Plant Protein Complexes

Global analyses of protein complex assembly, composition, and location are needed to fully understand how cells coordinate diverse metabolic, mechanical, and developmental activities. The most common methods for proteome-wide analysis of protein complexes rely on affinity purification-mass spectrometry or yeast two-hybrid approaches. These methods are time consuming and are not suitable for many plant species that are refractory to transformation or genome-wide cloning of open reading frames. Here, we describe the proof of concept for a method allowing simultaneous global analysis of endogenous protein complexes that begins with intact leaves and combines chromatographic separation of extracts from subcellular fractions with quantitative label-free protein abundance profiling by liquid chromatography-coupled mass spectrometry. Applying this approach to the crude cytosolic fraction of Arabidopsis thaliana leaves using size exclusion chromatography, we identified hundreds of cytosolic proteins that appeared to exist as components of stable protein complexes. The reliability of the method was validated by protein immunoblot analysis and comparisons with published size exclusion chromatography data and the masses of known complexes. The method can be implemented with appropriate instrumentation, is applicable to any biological system, and has the potential to be further developed to characterize the composition of protein complexes and measure the dynamics of protein complex localization and assembly under different conditions.     

《中国生物多样性保护战略与行动计划》的核心内容与实施战略

2010年9月15日,国务院第126次常务会议审议通过并发布实施《中国生物多样性保护战略与行动计划》(2011–2030年)(下称《战略与行动计划》)。该计划作为今后20年中国生物多样性保护的蓝图,需要强大的科学与技术支撑,这为生物学、生态学和环境科学等领域的广大科技工作者提供了一个广阔的空间。     

Achieving Target 2 of the Global Strategy for Plant Conservation: building a preliminary assessment of vascular plant species using data from herbarium specimens

The Global Strategy for Plant Conservation calls for a preliminary assessment of the conservation status of all known plant species by the year 2010. To date insufficient progress has been made on meeting this target. New efforts to develop a preliminary list beyond using the full IUCN criteria in plant assessments are needed. Here we present an algorithm that provides a preliminary assessment of the conservation status of plant species using data from herbarium specimens. We use Hawaiian specimen data from the United States National Herbarium to calibrate the parameters of the algorithm and then use specimen data from the Arecaceae, Commelinaceae, Gesneriaceae and Heliconiaceae as examples of the application of the algorithm. The algorithm was calibrated to insure 95% accuracy in placing the Hawaiian plant species into previously and independently determined threatened categories. Our results indicate that 28% of the Hawaiian taxa, 27% of the species of Arecaceae, 45% of the species of Commelinaceae, 32% of the species of Gesneriaceae, and 35% of the species of Heliconiaceae are Not Threatened and will not need any further evaluation for the preliminary assessment. Species identified here as Potentially Extinct and Potentially Threatened can be further assessed by additional herbarium material and/or conservation specialists for final evaluation using other assessment strategies (e.g., regional and national lists, taxonomic expert assessment, etc.).     

Moths count: recording moths for conservation in the UK

Insects are under-represented in current assessments of biodiversity loss at global and national scales. Butterflies, and a few other insect taxa, have been used as indicators of biodiversity change and as flagships for conservation, especially in temperate zones, but these groups are typically species-poor and may not be representative of insects as a whole. Macro-moths, on the other hand, are an important component of UK biodiversity, in terms of both species richness and ecosystem function. Moth abundance has decreased significantly in the UK and there is rapidly growing interest in the surveillance and monitoring of macro-moths among amateur and professional scientists. The Moths Count project was instigated to raise awareness of moths, increase moth recording and set up a long-term National Moth Recording Scheme for c.900 species of macro-moth in the UK. Since 2006, Moths Count has promoted moths to millions of people, trained and engaged thousands in recording and collated over eight million species distribution records into the National Moth Recording Scheme. These data, though incomplete at present, are already contributing to assessments of biodiversity loss and to conservation initiatives. We present initial results which show that substantial changes are already evident for macro-moths in the UK. The northern range margins of a sample of 12 species have shifted northwards by 194.8 km on average between 1982 and 2009 (7.8 km year^?1 over the 25 years between survey mid-points). Provisional distribution trends (not corrected for variation in recording effort) were calculated for 43 species and the results corroborated both positive and negative population-level changes assessed previously.     

Global Diversity and Conservation Priorities in the Cactaceae

The purpose of this paper was to analyze the diversity patterns of Cactaceae at a global scale, to identify those countries where conservation actions should be performed. In order to do this, the species richness and the number of endemic species for 34 American countries were determined. With these data, the relationship between the total number of species or the number of endemic species and the area of the countries were analyzed. In addition, a complementarity analysis was conducted to determine the most important countries for cactus conservation. Results showed that Mexico had the highest number of total and endemic species followed by Argentina, Bolivia, Brazil, and Peru, among others. There was a significant positive relationship between both, the total and endemic species, and the area of the countries. Despite this fact, the cactus diversity in Mexico, Argentina, Peru, Bolivia, Chile, and Costa Rica was higher than expected according to their area. Further, these countries also presented the highest proportions of endemic species. The complementarity analysis indicated that 24 countries are necessary to preserve all cactus species. However, 94% of all species could be preserved with only 10 countries. Considering the diversity patterns and the complementarity analysis, three important groups for cactus conservation were identified: (1) Mexico, Argentina, Peru, Bolivia, Chile, and Costa Rica, (2) Paraguay and Cuba, and (3) Brazil and USA. Conservation efforts should be focused on these countries in order to preserve cactus diversity.     

Plant Conservation Challenges in the Bahama Archipelago

The Bahamian archipelago has a rich flora with 89 endemic species. An international symposium held at Nassau in October 2012 to celebrate the 30th anniversary of the publication of the “Flora of the Bahama Archipelago” provided a forum to discuss plant conservation issues on these islands. This article builds on conclusions from this symposium and results from joint plant conservation research projects among the authors. The two main conservation challenges for these islands are: (1) environmental uncertainties derived from global warming and associated sea level changes and (2) the need for increased plant conservation awareness among the predominant urban population of the archipelago. Legal tools and biodiversity international agreements in place for The Commonwealth of the Bahamas can facilitate mechanisms for effective plant conservation. Further legal developments need to be established in The United Kingdom Overseas Territory of The Turks and Caicos Islands. There is an urgent need to redevelop the Botanic Garden of Nassau and designate it as the national botanic garden of The Bahamas. Further research related to the taxonomy, biology, conservation status, and distribution of the endemic species is urgently needed. Research initiatives pertinent to the detrimental effect and biology of invasive species are also lacking. The heterogeneous environments and uneven distribution of human populations across the archipelago are major challenges for conservation. Finally because of the political and economic status of The Bahamas and The Turks and Caicos, conservation agencies from the archipelago do not have easy access to international or British/European Union funds for global conservation initiatives.     

重庆市珍稀蝶类及保护对策

重庆市有 14种珍稀蝶类 ,主要分布在东北部、南部和东南部的 17个区 (市 )县 ,其中以江津、万盛、巫山、巫溪、城口、南川、彭水等 7个区 (市 )县最为重要。重庆市珍稀蝶类的保护对策是 ,加强法规制订和宣传 ,对濒危保护蝶类生物特性、生态学和行为学等方面进行专题研究 ,加强蝶类生栖地的保护和恢复 ,贯彻护·养·猎并举、以养蝶为主持续利用方针。     

Horse riding in urban conservation areas: Reviewing scientific evidence to guide management

Summary We undertook a literature review of the impacts of horse riding in conservation areas, and used it to guide management of horse riding in Canberra Nature Park (CNP), a large, fragmented semi‐natural park in and around the city of Canberra in the Australian Capital Territory. The literature review established that, because of their relatively large weight and small area in contact with the ground, horses have a relatively high potential for doing environmental damage. Impacts tend to be generally lowest for hikers, followed by motorcycles, horses and four‐wheeled vehicles. One study showed horse traffic caused more damage on established trails than motorcycles, off‐road bicycles or hikers. Most published studies of horse‐riding impacts in Australia have been conducted in alpine and subalpine environments, and in temperate woodlands and forests on sandstone near Sydney. They have shown that impacts are generally highest in previously untracked areas. Impacts on established trails are generally most marked on sections of trail that are wet, boggy or steep, and on unplanned and unmaintained trails. Impacts are lowest on constructed and maintained trails. Trail proliferation, associated with avoidance of untrafficable sections and short‐cutting, can be a major problem. Horses also have potential to spread weeds, because pastures and dried stock feeds contain large numbers of weed seeds that retain high levels of viability in horse manure. The risk of weed establishment is highest when manure is deposited in disturbed, damp sites, particularly when riding off‐track. Much less weed establishment is apparent when horse riders remain on‐track. Horse riding is a popular activity, but one that is relatively expensive to provide for, and one that may reduce opportunities for lower‐impact recreational park users. For all these reasons it appears socially equitable that provision is made for lower numbers of horse riders compared to numbers of park users involved in lower impact, more passive, recreational activities. We conclude by describing how this information was used to develop principles to guide management of horse riding and assess risk at individual sites in Canberra Nature Park.     

Plant species' response to climate change: implications for the conservation of European birds

Wildlife conservation faces new and extreme challenges in adapting to the accelerating dynamics of a world responding to global change. The Quaternary record shows that migration has been the usual response of organisms to environmental change. This record also reveals that forecast future climate changes are of a magnitude and in a direction unprecedented in recent earth history: the rate of these changes is likely also to surpass that of any comparable change during the last 2.4 million years.
The relationship between a species' geographical distribution and present climate may be modelled by a surface representing the probability of encountering that species under given combinations of climate conditions. This 'climate response surface' then may be used to simulate potential future distributions of the species in response to forecast climate scenarios. Such simulations reveal the magnitude of the impacts of these forecast climate changes. Although to date this approach has been applied in Europe only to plants, it promises to be valuable also for other groups of organisms, including birds. Some bird species, however, may respond more directly to either habitat structure or presence of specific food plants; such factors may be incorporated into the models when required.
The magnitude of likely vegetation changes necessitates a global approach to conservation if there is to be any hope of long-term success. Successful conservation of global biodiversity will depend upon conservation of the global environment and limitation of the human population much more than upon parochial efforts to conserve locally rare organisms or habitats.     

Using probability of persistence to identify important areas for biodiversity conservation

Most attempts to identify important areas for biodiversity have sought to represent valued features from what is known of their current distribution, and have treated all included records as equivalent. We develop the idea that a more direct way of planning for conservation success is to consider the probability of persistence for the valued features. Probabilities also provide a consistent basis for integrating the many pattern and process factors affecting conservation success. To apply the approach, we describe a method for seeking networks of conservation areas that maximize probabilities of persistence across species. With data for European trees, this method requires less than half as many areas as an earlier method to represent all species with a probability of at least 0.95 (where possible). Alternatively, for trials choosing any number of areas between one and 50, the method increases the mean probability among species by more than 10%. This improvement benefits the least-widespread species the most and results in greater connectivity among selected areas. The proposed method can accommodate local differences in viability, vulnerability, threats, costs, or other social and political constraints, and is applicable in principle to any surrogate measure for biodiversity value.     

CAPitalising on conservation knowledge: Using Conservation Action Planning,Healthy Country Planning and the Open Standards in Australia

More than 20 organisations use Conservation Action Planning (CAP), Healthy Country Planning and the Open Standards for the Practice of Conservation in over 140 projects, covering almost 160 million ha across Australia. This review documents the history, evolution and application of CAP in Australia and discusses its strengths, limitations and lessons learnt by users, including conservation planners, practitioners and policymakers.     

Plant Conservation in the Caribbean Island Biodiversity Hotspot

While the Caribbean is a recognized “biodiversity hotspot”, plant conservation has not received adequate attention; particularly, given the high levels of endemism in many plant groups. Besides establishing protected areas, there needs to be a sustained effort to study the taxonomy, systematics and ecology of the flora. Recent phylogenetic studies have shown high levels of endemism and conservation studies indicate a large propotion of the flora is threatened with extinction. Eight recommendations are given for plant conservation in the region.     

Using the IUCN Red List to determine effective conservation strategies

Threatened species lists continue to grow while the world’s governments fail to meet biodiversity conservation goals. Clearly, we are failing in our attempts to conserve biodiversity. Yet 37 mammal species genuinely improved in status in the 2009 IUCN Red List, suggesting there are ways to successfully conserve biodiversity. Here, I compare the threats and conservation actions (proposed and implemented) by the expert assessors of the Red List of improving species to a further 144 declining mammal species to determine whether specific threats were more easily remedied, and whether certain conservation actions were more successful than others. Declining species were faced with different threatening processes to mammals improving in status suggesting some threats were easier to treat (e.g. hunting) than others (climate change, invasive species). Declining species had different proposed and implemented conservation actions than improving species suggesting some actions are more successful than others. Threatened species were invariably found in conservation areas, suggesting protected area creation alone is not an overly successful strategy for species at risk of extinction. Conservation actions were more frequently implemented for improving than declining species suggesting active conservation is effective in improving the status of biodiversity. There were significant differences between proposed and implemented conservation actions suggesting some actions are easier to implement than others. Reintroduction, captive breeding and hunting restriction were more effective in conserving mammals than site creation and invasive species control. These findings highlight effective conservation actions for mammals worldwide and allow the rationalisation of threat mitigation measures to ensure economically justifiable biodiversity conservation strategies.