共查询到20条相似文献,搜索用时 31 毫秒
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Auxin [α-naphthaleneacetic acid (NAA) or indole-3-acetic acid] can induce the expression of lipoxygenases (LOXs) in cultured
immature zygotic embryo cotyledons of soybean [Glycine max. (L.) Merr]. These auxin-induced LOXs are different from those normally expressed in seeds but have the same isoelectric
points (pI) as those found in seedlings. The pIs of the two seedling LOXs were determined to be 5.09 and 5.23. One of the
auxin-induced LOXs has the same pI (5.09) and molecular mass (94 kDa) as seedling LOX4. The partial amino acid sequences from
the purified NAA-induced pI-5.09 LOX are identical to those of LOX4. RNA protection assays showed that NAA induces the expression
of LOX4 and LOX5 mRNAs in cultured embryo cotyledons where they are not normally expressed. Soybean genotypes with a polymorphic
variant of LOX4 in hypocotyls showed the same variation as NAA-induced LOXs in the embryo cotyledons. These results demonstrate
that the NAA-induced pI-5.09 LOX is seedling LOX4 and also suggest that auxin might be directly or indirectly involved in
seedling LOX expression during seed germination.
Received: 10 January 2000 / Revision received: 16 June 2000 / Accepted: 29 June 2000 相似文献
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John Webster 《BMJ (Clinical research ed.)》2000,320(7242):1149
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David Gullick 《BMJ (Clinical research ed.)》2001,322(7288):738
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Pamela Choudhury 《BMJ (Clinical research ed.)》1999,318(7175):61
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John Ives 《BMJ (Clinical research ed.)》1946,2(4478):667-668
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David Alan Walker, Emeritus Professor of Biology, University of Sheffield, UK and Fellow of the Royal Society, died on February 13, 2012. David had a marvelous 60?year career as a scientist, during which he was a researcher, mentor, valued colleague, and a prolific writer in the field of photosynthesis. His career was marked by creative breakthroughs in isolation and analysis of chloroplast metabolism in vitro and simple but valuable technical advances for measurement of photosynthesis in vivo that remain relevant on a global scale to production of crops and biofuels, as well as plant responses to climate change. We include here personal remembrances by the authors (GEE and UH), and by (in alphabetical order): Zoran Cerovic (France), Bob Furbank (Australia), Geoffrey Hind (USA), John Humby (UK), Agu Laisk (Estonia), Peter Lea (UK), Ross Lilley (Australia), Barry Osmond (Australia), Simon Robinson (Australia) and Charles Stirling (UK). 相似文献