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1.
The ongoing pandemic of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has placed a substantial strain on the supply of personal protective equipment, particularly the availability of N95 respirators for frontline healthcare personnel. These shortages have led to the creation of protocols to disinfect and reuse potentially contaminated personal protective equipment. A simple and inexpensive decontamination procedure that does not rely on the use of consumable supplies is dry heat incubation. Although reprocessing with this method has been shown to maintain the integrity of N95 respirators after multiple decontamination procedures, information on the ability of dry heat incubation to inactivate SARS-CoV-2 is largely unreported. Here, we show that dry heat incubation does not consistently inactivate SARS-CoV-2-contaminated N95 respirators, and that variation in experimental conditions can dramatically affect viability of the virus. Furthermore, we show that SARS-CoV-2 can survive on N95 respirators that remain at room temperature for at least five days. Collectively, our findings demonstrate that dry heat incubation procedures and ambient temperature for five days are not viable methods for inactivating SARS-CoV-2 on N95 respirators for potential reuse. We recommend that decontamination procedures being considered for the reuse of N95 respirators be validated at each individual site and that validation of the process must be thoroughly conducted using a defined protocol.  相似文献   

2.
Shortages of N95 respirators for use by medical personnel have driven consideration of novel conservation strategies, including decontamination for reuse and extended use. Decontamination methods listed as promising by the Centers for Disease Control and Prevention (CDC) (vaporous hydrogen peroxide (VHP), wet heat, ultraviolet irradiation (UVI)) and several methods considered for low resource environments (bleach, isopropyl alcohol and detergent/soap) were studied for two commonly used surgical N95 respirators (3M™ 1860 and 1870+ Aura™). Although N95 filtration performance depends on the electrostatically charged electret filtration layer, the impact of decontamination on this layer is largely unexplored. As such, respirator performance following decontamination was assessed based on the fit, filtration efficiency, and pressure drop, along with the relationship between (1) surface charge of the electret layer, and (2) elastic properties of the straps. Decontamination with VHP, wet heat, UVI, and bleach did not degrade fit and filtration performance or electret charge. Isopropyl alcohol and soap significantly degraded fit, filtration performance, and electret charge. Pressure drop across the respirators was unchanged. Modest degradation of N95 strap elasticity was observed in mechanical fatigue testing, a model for repeated donnings and doffings. CDC recommended decontamination methods including VHP, wet heat, and UV light did not degrade N95 respirator fit or filtration performance in these tests. Extended use of N95 respirators may degrade strap elasticity, but a loss of face seal integrity should be apparent during user seal checks. NIOSH recommends performing user seal checks after every donning to detect loss of appropriate fit. Decontamination methods which degrade electret charge such as alcohols or detergents should not be used on N95 respirators. The loss of N95 performance due to electret degradation would not be apparent to a respirator user or evident during a negative pressure user seal check.  相似文献   

3.
Reusing filtering facepiece respirators (FFRs) has been suggested as a strategy to conserve available supplies for home and healthcare environments during an influenza pandemic. For reuse to be possible, used FFRs must be decontaminated before redonning to reduce the risk of virus transmission; however, there are no approved methods for FFR decontamination. An effective method must reduce the microbial threat, maintain the function of the FFR, and present no residual chemical hazard. The method should be readily available, inexpensive and easily implemented by healthcare workers and the general public. Many of the general decontamination protocols used in healthcare and home settings are unable to address all of the desired qualities of an efficient FFR decontamination protocol. The goal of this study is to evaluate the use of two commercially available steam bags, marketed to the public for disinfecting infant feeding equipment, for FFR decontamination. The FFRs were decontaminated with microwave generated steam following the manufacturers' instructions then evaluated for water absorption and filtration efficiency for up to three steam exposures. Water absorption of the FFR was found to be model specific as FFRs constructed with hydrophilic materials absorbed more water. The steam had little effect on FFR performance as filtration efficiency of the treated FFRs remained above 95%. The decontamination efficacy of the steam bag was assessed using bacteriophage MS2 as a surrogate for a pathogenic virus. The tested steam bags were found to be 99.9% effective for inactivating MS2 on FFRs; however, more research is required to determine the effectiveness against respiratory pathogens.  相似文献   

4.
On June 11, 2009, the World Health Organization declared the outbreak of novel influenza A (H1N1) a pandemic. With limited supplies of antivirals and vaccines, countries and individuals are looking at other ways to reduce the spread of pandemic (H1N1) 2009, particularly options that are cost effective and relatively easy to implement. Recent experiences with the 2003 SARS and 2009 H1N1 epidemics have shown that people are willing to wear facemasks to protect themselves against infection; however, little research has been done to quantify the impact of using facemasks in reducing the spread of disease. We construct and analyze a mathematical model for a population in which some people wear facemasks during the pandemic and quantify impact of these masks on the spread of influenza. To estimate the parameter values used for the effectiveness of facemasks, we used available data from studies on N95 respirators and surgical facemasks. The results show that if N95 respirators are only 20% effective in reducing susceptibility and infectivity, only 10% of the population would have to wear them to reduce the number of influenza A (H1N1) cases by 20%. We can conclude from our model that, if worn properly, facemasks are an effective intervention strategy in reducing the spread of pandemic (H1N1) 2009.  相似文献   

5.
The aim of this study was to develop a test system to evaluate the effectiveness of procedures for decontamination of respirators contaminated with viral droplets. MS2 coliphage was used as a surrogate for pathogenic viruses. A viral droplet test system was constructed, and the size distribution of viral droplets loaded directly onto respirators was characterized using an aerodynamic particle sizer. The sizes ranged from 0.5 to 15 μm, and the sizes of the majority of the droplets were the range from 0.74 to 3.5 μm. The results also showed that the droplet test system generated similar droplet concentrations (particle counts) at different respirator locations. The test system was validated by studying the relative efficiencies of decontamination of sodium hypochlorite (bleach) and UV irradiation with droplets containing MS2 virus on filtering facepiece respirators. It was hypothesized that more potent decontamination treatments would result in corresponding larger decreases in the number of viable viruses recovered from the respirators. Sodium hypochlorite doses of 2.75 to 5.50 mg/liter with a 10-min decontamination period resulted in approximately 3- to 4-log reductions in the level of MS2 coliphage. When higher sodium hypochlorite doses (≥8.25 mg/liter) were used with the same contact time that was used for the dilute solutions containing 2.75 to 5.50 mg/liter, all MS2 was inactivated. For UV decontamination at a wavelength of 254 nm, an approximately 3-log reduction in the level of MS2 virus was achieved with dose of 4.32 J/cm2 (3 h of contact time with a UV intensity of 0.4 mW/cm2), while with higher doses of UV irradiation (≥7.20 J/cm2; UV intensity, 0.4 mW/cm2; contact times, ≥5 h), all MS2 was inactivated. These findings may lead to development of a standard method to test decontamination of respirators challenged by viral droplets.During an infectious disease outbreak widespread panic can result from a limited understanding of the transmission route. Although some research points to a larger role for droplet nuclei (21, 28), other research suggests that droplets are the principal means of transmitting respiratory infections (10, 26). Droplets containing an infectious microorganism are believed to be transmitted to individuals who directly inhale the droplets resulting from coughing by carriers in close proximity or who ingest droplets spread to the mouth or the nose via the hands (7, 15). Large droplets were first defined as droplets more than 100 μm in diameter by Wells (30). Elsewhere, however, droplets more than 5 μm (23) or 10 μm (8) in diameter are often treated as large droplets. In this paper, the term “viral droplet” refers to all virus-containing liquid particles that retain their original size without significant evaporation, regardless of their specific size.N95 filtering facepiece respirators (FFRs) are routinely employed to prevent exposure of workers to biological hazards such as severe acute respiratory syndrome, tuberculosis, and novel H1N1 influenza A (6). In addition to CDC interim guidance (6), a recent report from the National Academies'' Institute of Medicine suggests that healthcare workers who are in close contact with individuals with novel H1N1 influenza illnesses should use fit-tested N95 respirators to reduce the risk of infection (18). This report also recommends increased research on influenza transmission and respiratory protection, which would enable policy makers to update these types of recommendations as additional disease prevention data become available.Current best practices suggest that once an FFR is worn in the presence of an infected patient, it should be considered potentially contaminated and discarded (5). However, during a pandemic outbreak a shortage of FFRs could occur (4). According to another report from the Institute of Medicine, during a 42-day influenza pandemic outbreak over 90 million N95 FFRs will be needed to protect workers in the healthcare sector (4). Furthermore, this report suggested that FFR reuse following decontamination should be considered a possible solution to deal with anticipated FFR shortages. Low-temperature biological decontamination methods have been suggested as a possible solution, but additional research needs to be done to determine whether infectious organisms can survive the decontamination process and if the decontamination method changes respirator fit (29).While it is well known that droplets play a role in the transmission of some respiratory infections, there is a lack of knowledge and data on the effectiveness of decontamination methods applied to respirators and porous personal protective equipment. There are several test methods for evaluation of the effectiveness of decontamination procedures for liquids and for hard porous or nonporous surfaces when they are challenged with viruses (2, 3, 27), while other methods are used to assess the effectiveness of decontamination procedures for FFRs when they are challenged with viral droplet nuclei (12). However, there is no test method to evaluate the effectiveness of biological decontamination procedures for disposable FFRs after they are challenged with viral droplets (liquid droplets containing a virus) whose sizes are similar to the sizes of droplets expelled by humans.Therefore, the aim of the present study was to develop a test system to evaluate the effectiveness of procedures for decontamination of respirators contaminated with viral droplets. The system was validated using two possible decontamination strategies: sodium hypochlorite and UV irradiation. It was hypothesized that the more potent decontamination treatments would result in corresponding larger decreases in the number of viable viruses recovered from the respirators than the less aggressive treatments.  相似文献   

6.
Aims: To develop a method to assess model‐specific parameters for ultraviolet‐C (UV‐C, 254 nm) decontamination of filtering facepiece respirators (FFRs). Methods and Results: UV‐C transmittance was quantified for the distinct composite layers of six N95 FFR models and used to calculate model‐specific α‐values, the percentage of the surface UV‐C irradiance available for the internal filtering medium (IFM). Circular coupons, excised from the FFRs, were exposed to aerosolized particles containing MS2 coliphage and treated with IFM‐specific UV‐C doses ranging from 38 to 4707 J m?2. Models exposed to a minimum IFM dose of 1000 J m?2 demonstrated at least a 3 log reduction (LR) in viable MS2. Model‐specific exposure times to achieve this IFM dose ranged from 2 to 266 min. Conclusions: UV‐C transmits into and through FFR materials. LR of MS2 was a function of model‐specific IFM UV‐C doses. Significance and Impact of the Study: Filtering facepiece respirators are in high demand during infectious disease outbreaks, potentially leading to supply shortages. Reuse of disposable FFRs after decontamination has been discussed as a possible remediation strategy, but to date lacks supporting scientific evidence. The methods described here can be used to assess the likelihood that UV‐C decontamination will be successful for specific FFR models.  相似文献   

7.

Background

Governments are preparing for a potential influenza pandemic. Therefore they need data to assess the possible impact of interventions. Face-masks worn by the general population could be an accessible and affordable intervention, if effective when worn under routine circumstances.

Methodology

We assessed transmission reduction potential provided by personal respirators, surgical masks and home-made masks when worn during a variety of activities by healthy volunteers and a simulated patient.

Principal Findings

All types of masks reduced aerosol exposure, relatively stable over time, unaffected by duration of wear or type of activity, but with a high degree of individual variation. Personal respirators were more efficient than surgical masks, which were more efficient than home-made masks. Regardless of mask type, children were less well protected. Outward protection (mask wearing by a mechanical head) was less effective than inward protection (mask wearing by healthy volunteers).

Conclusions/Significance

Any type of general mask use is likely to decrease viral exposure and infection risk on a population level, in spite of imperfect fit and imperfect adherence, personal respirators providing most protection. Masks worn by patients may not offer as great a degree of protection against aerosol transmission.  相似文献   

8.
Decontamination of N95 filtering facepiece respirators (FFRs) is a crisis capacity strategy allowed when there are known shortages of FFRs. The application of moist heat is one decontamination method that has shown promise and is the approach approved in the Steris Steam Emergency Use Authorization (EUA). This effort examines the use of multicookers to apply moist heat, as they are available in retail stores and more affordable than methods requiring more sophisticated equipment. Four of five multicooker models examined met the acceptance criteria for the test and one model was selected for inactivation testing. Tests were performed on four different FFR models with SARS-CoV-2 suspended in culture media, simulated saliva or simulated lung fluid. Moist heat treatment reduced recoverable titres of SARS-CoV-2 virus to levels below the limit of detection in all tests. Furthermore, these four FFR models showed no loss in collection efficiency, inhalation resistance or visual damage after up to 10 decontamination cycles. Two (2) FFR models showed a slight change in strap elasticity (<9%). These data show that moist heat treatment using a multicooker is a viable option for FFR decontamination in a crisis capacity strategy.  相似文献   

9.
The person-to-person transmission of influenza virus, especially in the event of a pandemic caused by a highly virulent strain of influenza, such as H5N1 avian influenza, is of great concern due to widespread mortality and morbidity. The consequences of seasonal influenza are also substantial. Because airborne transmission appears to play a role in the spread of influenza, public health interventions should focus on preventing or interrupting this process. Air disinfection via upper-room 254-nm germicidal UV (UV-C) light in public buildings may be able to reduce influenza transmission via the airborne route. We characterized the susceptibility of influenza A virus (H1N1, PR-8) aerosols to UV-C light using a benchtop chamber equipped with a UVC exposure window. We evaluated virus susceptibility to UV-C doses ranging from 4 to 12 J/m(2) at three relative humidity levels (25, 50, and 75%). Our data show that the Z values (susceptibility factors) were higher (more susceptible) to UV-C than what has been reported previously. Furthermore, dose-response plots showed that influenza virus susceptibility increases with decreasing relative humidity. This work provides an essential scientific basis for designing and utilizing effective upper-room UV-C light installations for the prevention of the airborne transmission of influenza by characterizing its susceptibility to UV-C.  相似文献   

10.
COVID-19 appeared in December 2019, needing efforts of science. Besides, a range of light therapies (photodynamic therapy, ultraviolet [UV], laser) has shown scientific alternatives to conventional decontamination therapies. Investigating the efficacy of light-based therapies for environment decontamination against SARS-CoV2, a PRISMA systematic review of Phototherapies against SARS-CoV or MERS-CoV species discussing changes in viral RT-PCR was done. After searching MEDLINE/PubMed, EMBASE, and Literatura Latino-Americana e do Caribe em Ciências da Saúde we have found studies about cell cultures irradiation (18), blood components irradiation (10), N95 masks decontamination (03), inanimate surface decontamination (03), aerosols decontamination (03), hospital rooms irradiation (01) with PDT, LED, and UV therapy. The best quality results showed an effective low time and dose UV irradiation for environments and inanimate surfaces without human persons as long as the devices have safety elements dependent on the surfaces, viral charge, humidity, radiant exposure. To interpersonal contamination in humans, PDT or LED therapy seems very promising and are encouraged.  相似文献   

11.
Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is an RNA virus that causes coronavirus infection (COVID‐19). COVID‐19 is a highly contagious disease transmitted through respiratory droplets, saliva and other contact routes. Within 10 months of its outbreak, SARS‐CoV‐2 has infected more than 23 million people around the world. Evidence suggests that older adults are the most vulnerable to infection and have an increased risk of mortality. Reduced immunity and underlying medical conditions make them risk‐prone and vulnerable to critical care. Older adults affected with the SARS‐CoV‐2 virus present with distinct clinical manifestations necessitating specific treatment needs and management protocols. While it is crucial to prevent the spread of novel coronavirus (2019‐nCoV), the role of oral healthcare workers in addressing the specific needs of ageing adult patients by adopting specific guidelines and appropriate infection control protocols is timely. This paper aims to develop specific guidelines and protocols for the dental management of geriatric patients during the COVID‐19 pandemic.  相似文献   

12.
Song  Zhi-Gang  Chen  Yan-Mei  Wu  Fan  Xu  Lin  Wang  Bang-Fang  Shi  Lei  Chen  Xiao  Dai  Fa-Hui  She  Jia-Lei  Chen  Jian-Min  Holmes  Edward C.  Zhu  Tong-Yu  Zhang  Yong-Zhen 《中国病毒学》2020,35(6):785-792
Virologica Sinica - Healthcare workers (HCWs) are at high risk of occupational exposure to the new pandemic human coronavirus, SARS-CoV-2, and are a source of nosocomial transmission in airborne...  相似文献   

13.
Netherlands Heart Journal - In the Netherlands, the coronavirus disease 2019 (COVID?19) pandemic has resulted in excess mortality nationwide. Chronic heart disease patients are at risk for...  相似文献   

14.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causing the global coronavirus disease 2019 (COVID-19) pandemic. Because complete elimination of SARS-CoV-2 appears difficult, decreasing the risk of transmission is important. Treatment with 0.1 and 0.05 ppm ozone gas for 10 and 20 hr, respectively, decreased SARS-CoV-2 infectivity by about 95%. The magnitude of the effect was dependent on humidity. Treatment with 1 and 2 mg/L ozone water for 10 s reduced SARS-CoV-2 infectivity by about 2 and 3 logs, respectively. Our results suggest that low-dose ozone, in the form of gas and water, is effective against SARS-CoV-2.  相似文献   

15.
Molecular Biology - The pandemic of coronavirus disease 2019 (COVID-19) warrants the identification of factors that may determine both risk and severity of infection. The factors include microRNAs...  相似文献   

16.
口罩是预防呼吸道暴露的重要防线,正确选择和佩戴口罩可以降低新型冠状病毒(2019 novel coronavirus,2019-nCoV)等呼吸道传播病原感染的风险。但是,口罩类型、防护等级、职业性质、工作环境和防护要求以及正确的佩戴方式均会影响防护效果。本文以国家标准、行业标准以及诊疗方案等资料为参照,针对不同职业人群对口罩的选择与佩戴的关键点展开论述,为不同人群正确使用口罩提供参考,降低各类人群的暴露风险,以利于疫情的控制。  相似文献   

17.
目的 了解医院应对流感大流行的能力。方法 通过现场调查方法调查6家二级及以上医院和5家一级医院。结果 (1)二级及以上医院呼吸相关科室医生218人(14.15 %),护士314人(19.26%),病床数469张(19.68%),呼吸机27台(36.99%);一级医院呼吸相关科室医生29人(14.79%),护士24人(35.29 %),病床数53张(22.27%); 呼吸相关科室医护人员比例除1家外,其余均低于1:2; 2家医院ICU病床数占整个医院总床数比例低于2%。(2)5家二级及以上医院设置了呼吸相关科室,5家一级医院设置了传染病接诊室。所有医院均能提供口罩和手套,棉纱口罩和N95口罩各有4家医院(36.36%)能够提供,部分医院能够提供洗手设备和洗手用品;2家医院为职工(18.18%)医院接种流感疫苗。(3)10家(91.91%)医院只储备部分个人防护用品,3家(27.27%)储备抗病毒药物。结论 目前医院应对流感大流行的能力有限。  相似文献   

18.

Background

Though recommended by many and mandated by some, influenza vaccination rates among health care workers, even in pandemics, remain below optimal levels. The objective of this study was to assess vaccination uptake, attitudes, and distinguishing characteristics (including doctor-nurse differences) of health care workers who did and did not receive the pandemic H1N1 influenza vaccine in late 2009.

Methodology/Principal Findings

In early 2010 we mailed a self-administered survey to 800 physicians and 800 nurses currently licensed and practicing in Minnesota. 1,073 individuals responded (cooperation rate: 69%). 85% and 62% of Minnesota physicians and nurses, respectively, reported being vaccinated. Accurately estimating the risk of vaccine side effects (OR 2.0; 95% CI 1.5–2.7), agreeing with a professional obligation to be vaccinated (OR 10.1; 95% CI 7.1–14.2), an ethical obligation to follow public health authorities'' recommendations (OR 9.9; 95% CI 6.6–14.9), and laws mandating pandemic vaccination (OR 3.1; 95% CI 2.3–4.1) were all independently associated with receiving the H1N1 influenza vaccine.

Conclusions/Significance

While a majority of health care workers in one midwestern state reported receiving the pandemic H1N1 vaccine, physicians and nurses differed significantly in vaccination uptake. Several key attitudes and perceptions may influence health care workers'' decisions regarding vaccination. These data inform how states might optimally enlist health care workers'' support in achieving vaccination goals during a pandemic.  相似文献   

19.
The ongoing pandemic of coronavirus disease 2019(COVID-19)caused by a novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2,also named as 2019-nCoV or HCoV-19)poses an unprecedented threat to public health(Zhu et al.,2020;Wang et al.,2020;Jiang et al.,2020).The novel HCoV-19 virus has rapidly spread into multiple countries across the world since it was first reported in December 2019.The World Health Organization(WHO)declared COVID-19 as a pandemic on 11th March 2020.As of 4th July,over 10 million confirmed COVID-19 cases have been reported in over 200 countries/regions with more than 0.5 million deaths,including 85,287 documented cases and 4,648 deaths in China(WHO,2020a).  相似文献   

20.
The efficacy of powered air-purifying respirators, surgical masks, dust/mist respirators, and high-efficiency respirators were tested with a biological aerosol under simulated breathing conditions. Protective ability ranged from 67 to 99.95%. The majority of penetration in negative-pressure respirators occurred at the face-mask interface rather than through the filter material.  相似文献   

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