全蝎软膏对大鼠糖尿病皮肤溃疡创面愈合的效果评价

目的:探讨全蝎软膏治疗糖尿病皮肤创面愈合的作用及其机制。方法:采用SPF级SD大鼠构建糖尿病皮肤溃疡模型,将其随机平均分为对照组、模型组、全蝎软膏组和湿润烧伤膏组。造模后,分别在创面处涂抹全蝎软膏或湿润烧伤膏处理,空白组和模型组用PBS处理,观察和比较各组创面的愈合情况,并收集用药后3 d、7 d、10 d和14 d的创面肉芽组织进行HE染色,通过ELISA及荧光定量PCR检测肉芽组织内碱性成纤维细胞生长因子(bFGF)、血管性假血友病因子(v WF)、肿瘤坏死因子α(TNF-α)与Smad 4基因的表达。结果:成功构建大鼠糖尿病皮肤溃疡模型,成模率为96.15%。创伤后第3 d、7 d、10 d和14 d,各治疗组创面愈合率均显著高于模型组(P0.05),给药14 d时,全蝎软膏组创面愈合率已接近对照组水平。HE染色结果显示与模型组相比,全蝎软膏组中肉芽组织生长较快、毛细血管数量增多且组织纤维化程度较低。ELISA和荧光定量PCR检测结果显示与模型组和湿润烧伤膏组相比,全蝎软膏组肉芽组织内bFGF、v WF含量上调更显著(P0.05),而TNF-α含量与Smad 4 m RNA显著降低(P0.05)。结论:全蝎软膏可促进糖尿病皮肤溃疡创面肉芽组织的生长,可能与其抑制炎症反应并改善血管功能有关。     

Vagotomy for Gastric Ulcer

Out of a series of 75 patients who were treated by vagotomy and drainage for gastric ulcer 66 have been followed up for one to seven years (average three-and-a-half years). Four recurrent gastric ulcers occurred among the 33 cases of ulcer of the lesser curve or body with no antral or duodenal lesion (type I). Among the 42 patients with an antral ulcer or an associated active or healed pyloric or duodenal lesion (type II or III) there was only one recurrence. The recurrence rate for men was 5% and for women 12%. One patient died two years after operation from cancer which may have originated in the stomach. The clinical results in patients without recurrences were generally satisfactory.Vagotomy, preferably selective, with drainage is a satisfactory operation in gastric ulcers of types II and III. For ulcers of type I, gastrectomy is to be preferred except in selected cases.     

全蝎软膏对糖尿病皮肤溃疡大鼠模型EGF、PDGF及其受体表达的影响

摘要 目的：探讨全蝎软膏对糖尿病皮肤溃疡大鼠模型EGF、PDGF及其受体表达的影响及创面愈合的机理。方法：60只大鼠随机分为对照组、模型组、湿润烧伤膏组和全蝎软膏组,每组15只。建立糖尿病皮肤溃疡大鼠模型,干预后7天及14天,观察并比较大鼠创面愈合率和血清中表皮生长因子(epidenllal giiilllh factor, EGF)、血小板衍生生长因子(platelet -derived growth factor, PDGF)及其受体水平。结果：全蝎软膏组、湿润烧伤膏组与模型组7 d、14 d同时间点比较创面愈合率,差异有统计学意义(P＜0.05或 P＜0.01);全蝎软膏组、湿润烧伤膏组7 d、14 d同时间点比较,差异无统计学意义(P＞0.05)。免疫组化结果显示,EGF的表达水平,全蝎软膏组、湿润烧伤膏组与模型组比较,7 d差异有统计学意义(P＜0.05);14 d差异无统计学意义(P＞0.05)。EGFR 的表达水平,全蝎软膏组与湿润烧伤膏组、模型组、空白组7 d、14 d同时间点比较,差异无统计学意义(P＞0.05)。PDGFB的表达水平,全蝎软膏组、湿润烧伤膏组与模型组7 d、14 d同时间点比较,差异有统计学意义(P＜0.05)。PDGFRB的表达水平,7 d全蝎软膏组与湿润烧伤膏组、模型组、空白组比较,差异无统计学意义(P＞0.05);14 d全蝎软膏组、湿润烧伤膏组与模型组比较,差异有统计学意义(P＜0.05或P＜0.01)。结论：全蝎软膏可影响糖尿病皮肤溃疡大鼠模型EGF、PDGF及其受体的表达,促进糖尿病创面愈合。