Altererythrobacter flava sp. nov., a new member of the family Erythrobacteraceae,isolated from a surface seawater sample

A Gram-stain-negative, light yellow pigmented, non-motile and aerobic bacterial strain, designated HHU E2-1 ^T, was isolated from a surface seawater sample. The 16S rRNA gene sequence analysis indicated that HHU E2-1 ^T shared the highest sequence similarity to the type strain Qipengyuania gaetbuli DSM 16225 ^T (96.90%), which belongs to the family Erythrobacteraceae. Combined phylogeny of 288 single-copy orthologous gene clusters, analysis of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), average amino acid identity (AAI) and evolutionary distances suggested that HHU E2-1 ^T can be considered as a member of the genus Altererythrobacter based on the recently proposed standard for defining genera of Erythrobacteraceae. Strain HHU E2-1 ^T grew at 15–35 °C and pH 5.0–8.0, with optimum growth at 28 °C and pH 7.0. Tolerance to NaCl was up to 4% (w/v) with optimum growth in 2–3% NaCl. The major fatty acids (>?10%) were C_18:1ω7c11-methyl, summed feature 3 (C_16:1ω7c and/or C_16:1ω6c), and summed feature 8 (C_18:1ω7c and/or C_18:1ω6c). The predominant isoprenoid quinone was ubiquinone-10. The genomic G?+?C content was 57.40%. On the basis of the phenotypic, phylogenetic and chemotaxonomic characterizations, HHU E2-1 ^T represents a novel species of the genus Altererythrobacter, for which the name Altererythrobacter flava sp. nov. is proposed. The type strain is HHU E2-1 ^T (=?CGMCC 1.17394 ^T?=?KCTC 72835 ^T?=?MCCC 1K04226^T).

     

Genome mining revealed polyhydroxybutyrate biosynthesis by Ramlibacter agri sp. nov., isolated from agriculture soil in Korea

A white-colony-forming, facultative anaerobic, motile and Gram-stain-negative bacterium, designated G-1-2-2 ^T was isolated from soil of agriculture field near Kyonggi University, Republic of Korea. Strain G-1-2-2 ^T synthesized the polyhydroxybutyrate and could grow at 10–35 °C. The phylogenetic analysis based on 16S rRNA gene sequence showed that, strain G-1-2-2 ^T formed a lineage within the family Comamonadaceae and clustered as a member of the genus Ramlibacter. The 16S rRNA gene sequence of strain G-1-2-2 ^T showed high sequence similarities with Ramlibacter ginsenosidimutans BXN5-27 ^T (97.9%), Ramlibacter monticola G-3-2 ^T (97.9%) and Ramlibacter alkalitolerans CJ661^T (97.5%). The sole respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, and an unidentified phospholipid. The principal cellular fatty acids were C_16:0, cyclo-C_17:0, summed feature 3 (C_16:1ω7c and/or C_16:1ω6c) and summed feature 8 (C_18:1ω7c and/or C_18:1ω6c). The genome of strain G-1-2-2 ^T was 7,200,642 bp long with 13 contigs, 6,647 protein-coding genes, and DNA G?+?C content of 68.9%. The average nucleotide identity and in silico DNA–DNA hybridization values between strain G-1-2-2 ^T and close members were?≤?81.2 and 24.1%, respectively. The genome of strain G-1-2-2 ^T showed eight putative biosynthetic gene clusters responsible for various secondary metabolites. Genome mining revealed the presence of atoB, atoB2, phaS, phbB, phbC, and bhbD genes in the genome which are responsible for polyhydroxybutyrate biosynthesis. Based on these data, strain G-1-2-2 ^T represents a novel species in the genus Ramlibacter, for which the name Ramlibacter agri sp. nov. is proposed. The type strain is G-1-2-2 ^T (=?KACC 21616 ^T?=?NBRC 114389 ^T).

     

Microvirga splendida sp. nov., bacteria isolated from soil

Two bacterial strains, BT325^T and BT690, were isolated from soil samples collected in Korea. Both strains were Gram stain-negative, short rod-shaped, and formed light-pink colored colonies. The 16S rRNA sequence similarity of strains BT325^T and BT690 shared a sequence similarity of 99.7%. Both strains shared the highest 16S rRNA gene similarity of 98.6% with Microvirga arabica SV2184P^T, followed by Microvirga ossetica V5/3 M T (98.5% and 98.2%, respectively), Microvirga soli R491^T (98.3% and 98.2%, respectively), Microvirga aerilata (98.2% and 98.08%, respectively), Microvirga makkahensis (98.08% and 97.8%, respectively). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BT325^T and BT690 were positioned in a distinct lineage within the family Methylobacteriaceae (order Rhizobiales, class Alphaproteobacteria). The genome size of strain BT325^T was 5,200,315 bp and the genomic DNA G?+?C content was 64.3 mol%. The sole respiratory quinone of strain BT325^T was Q-10 and the predominant cellular fatty acids were summed feature 3 (C_16:1 ω7c/C_16:1 ω6c) and summed feature 8 (C_18:1 ω7c/C_18:1 ω6c). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. Polyphasic taxonomic analysis of biochemical, chemotaxonomic, and phylogenetic analyses suggested that strains BT325^T represents a novel bacterial species within the genus Microvirga, for which the name Microvirga splendida is proposed. The type strain of Microvirga splendida is BT325^T (=?KCTC 72406 ^T?=?NBRC 114847 ^T).

     

<Emphasis Type="Italic">Photobacterium atrarenae</Emphasis> sp. nov. a Novel Bacterium Isolated from Sea Sand

The gram-reaction-negative, motile, facultatively anaerobic, catalase-positive, oxidase-positive bacterial strain M3-4^T was isolated from black sea sand and subjected to a taxonomic study. Cells of strain M3-4^T have monotrichous flagella, grow optimally at 37°C and at pH 7–8 in the presence of 1–4% (w/v) NaCl and hydrolyze casein, starch and l-tyrosine. According to phylogenetic analyses using 16S rRNA gene sequences, strain M3-4^T belongs to the genus Photobacterium and is most closely related to Photobacterium rosenbergii LMG 22223^T (97.4%) and P. gaetbulicola KCTC 22804^T (96.6%). The DNA–DNA relatedness value between M3-4^T and P. rosenbergii LMG 22223^T was 21.5%. The DNA G+C mol% of strain M3-4^T was 53.6. The major cellular fatty acid of strain M3-4^T was a summed feature 3 consisting of C_16:1 ω7c and/or iso-C_15:0 2-OH (35.0%), followed by C_16:0 (25.4%) and C_18:1ω7c (16.8%). These data suggest that strain M3-4^T represents a novel species in genus Photobacterium, for which the name P. atrarenae sp. nov. is proposed. The type strain is M3-4^T (= KCTC 23265^T = NCAIM B 02414^T).     

Oryzicola mucosus gen. nov., sp. nov., a novel slime producing bacterium belonging to the family Phyllobacteriaceae isolated from the rhizosphere of rice plants

A novel Gram-stain negative, asporogenous, slimy, rod-shaped, non-motile bacterium ROOL2^T was isolated from the root samples collected from a rice field located in Ilsan, South Korea. Phylogenetic analysis of the 16S rRNA sequence showed 96.5% similarity to Tianweitania sediminis Z8^T followed by species of genera Mesorhizobium (96.4–95.6%), Aquabacterium (95.9–95.7%), Rhizobium (95.8%) and Ochrobactrum (95.6%). Strain ROOL2^T grew optimally at 30 °C in the presence of 1–6% (w/v) NaCl and at pH 7.5. The major respiratory quinone was ubiquinone-10 and the major cellular fatty acids were C_18:1ω7c, summed feature 4 (comprising iso-C_17:1 I and/or anteiso-C_17:1 B) and summed feature 8 (comprising C_18:1ω6c and/or C_18:1ω7c). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylglycerol, one unidentified aminolipid and two unidentified lipids. The assembled draft genome of strain ROOL2^T had 28 contigs with N50 value of 656,326 nt, total length of 4,894,583 bp and a DNA G?+?C content of 61.5%. The average amino acid identity (AAI) values of strain ROOL2^T against the genomes of related members belonging to the same family were below 68% and the ANI and dDDH values between the strain ROOL2^T and the type strains of phylogenetically related species were 61.8–76.3% and 19.4–21.1%, respectively. Strain ROOL2^T only produces carotenoid-type pigment when grown on LB agar and slime on R2A agar. In the presence of tryptophan, strain ROOL2^T produced indole acetic acid (IAA), a phytohormone in plant growth and development. Gene clusters for indole-3-glycerol phosphatase and tryptophan synthase were found in the genome of strain ROOL2^T. The genotypic and phenotypic characteristics indicated that strain ROOL2^T represents a novel genus belonging the family Phyllobacteriaceae, for which the name Oryzicola mucosus gen. nov., sp. nov. is proposed. The type strain is ROOL2^T (KCTC 82711 ^T?=?NBRC 114717 ^T).

     

Pantoea endophytica sp. nov., novel endophytic bacteria isolated from maize planting in different geographic regions of northern China

Four endophytic bacterial strains were isolated from root, stem and leaf of maize planted in different regions of northern China. The four strains possessed almost identical 16S rRNA gene sequences. However, REP-PCR fingerprint patterns discriminated that they were not from one clonal origin. Furthermore, the average nucleotide identity (ANI) values among them were higher than 95%, suggesting they all belong to one species. Based on 16S rRNA gene phylogeny, the four strains were clustered together with Pantoea rodasii LMG 26273^T and Pantoea rwandensis LMG 26275^T, but on a separate branch. Multilocus sequence analysis (MLSA) indicated that the four strains form a novel Pantoea species. Authenticity of the novel species was confirmed by ANI comparisons between strain 596^T and its closest relatives, since obtained values were considerably below the proposed thresholds for the species delineation. The genome size of 596^T was 5.1Mbp, comprising 4896 predicted genes with DNA G + C content of 57.8 mol%. The respiratory quinone was ubiquinone-8 (Q-8) and the polar lipid profile consisted of phosphatidylethanolamin, diphosphatidylglycerol, phosphatidylglycerol, unidentified aminophospholipid and unidentified phospholipid. The major fatty acids of strain 596^T were C_16:0, summed feature 2 (C_12:0 aldehyde), summed feature 3 (C_16:1ω7c and/or C_16:1ω6c) and summed feature 8 (C_18:1ω7c and/or C_18:1ω6c). Based on phylogenetic, genomic, phenotypic and chemotaxonomic data, the four isolates are considered to represent a novel species of the genus Pantoea, for which the name Pantoea endophytica sp. nov., is proposed, with 596^T (= DSM 100,785^T = CGMCC 1.15280^T) as type strain.     

Hymenobacter taeanensis sp. nov., radiation resistant bacterium isolated from coastal sand dune

An aerobic, Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, and light pink-colored bacterial strain, designated TS19^T, was isolated from a sand sample obtained from a coastal sand dune after exposure to 3 kGy of gamma radiation. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the isolate was a member of the genus Hymenobacter and was most closely related to H. wooponensis WM78^T (98.3% similarity). Strain TS19^T and H. wooponensis showed resistance to gamma radiation with D₁₀ values (i.e., the dose required to reduce the bacterial population by tenfold) of 7.3 kGy and 3.5 kGy, respectively. The genome of strain TS19^T consists of one contig with 4,879,662 bp and has a G?+?C content of 56.2%. The genome contains 3,955 protein coding sequences, 44 tRNAs, and 12 rRNAs. The predominant fatty acids of strain TS19^T were iso-C_15:0, summed feature 4 (iso-C_17:1 I and/or anteiso-C_17:1 B), summed feature 3 (C_16:1 ω6c and/or C_16:1 ω7c), and C_16:1 ω5c. The major polar lipids were phosphatidylethanolamine, and one unidentified aminophospholipid. The main respiratory quinone was menaquinone-7. Based on the phylogenetic, physiological, and chemotaxonomic characteristics, strain TS19^T represents a novel species, for which the name Hymenobacter taeanensis sp. nov. is proposed. The type strain is TS19^T (=?KCTC 72897^T?=?JCM 34023^T).

     

Shinella oryzae sp. nov., a novel zearalenone-resistant bacterium isolated from rice paddy soil

A novel bacterium, designated Z-25 ^T, was isolated from a rice paddy rhizosphere soil sample from Wuchang County, China. The Z-25 ^T strain is gram-negative, rod-shaped, non-spore-forming, aerobic, motile by unipolar flagella and straw white in color. A phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain Z-25 belongs to the genus Shinella, and the closest members are Shinella zoogloeoides ATCC 19623 ^T with 98.58% similarity, S. kummerowiae CCBAU 25,048 T (98.03%) and S. granuli Ch06 ^T (97.37%). The average nucleotide identity and in silico DNA-DNA hybridization values between strain Z-25 ^T and the closest members were less than 85.29% and 28.70%, respectively. The predominant fatty acids were the sums of features comprising C_18:1 ω7c and/or C_18:1 ω6c (34.62%), C_18:1 ω7c -11-methyl (20.48%), and C_19:0 cyclo ω8c (18.19%). The only respiratory quinone was ubiquinone-10, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Additionally, a genome analysis showed that Z-25 ^T presented potential functional genes related to the degradation of zearalenone (ZEN). An HPLC analysis indicated that Z-25 ^T could remove 74.13% of 10 mg/L ZEN after 144 h at 30 °C. Therefore, based on phenotypic, chemotaxonomic, phylogenetic and genotypic analyses, strain Z-25 ^T represents a novel species in the genus Shinella, for which the name Shinella oryzae sp. nov. is proposed. The type strain is Z-25 ^T (=?GDMCC 1.2424 ^T?=?KCTC 82660 ^T).

     

<Emphasis Type="Italic">Joostella atrarenae</Emphasis> sp. nov., a novel member of the <Emphasis Type="Italic">Flavobacteriaceae</Emphasis> originating from the black sea sand of Jeju Island

Strain M1-2^T was isolated from the black sand from the seashore of Jeju Island, Republic of Korea and was classified using a polyphasic taxonomic approach. Strain M1-2^T appeared as Gram-negative, motile rods that could grow in the presence of 1–10% (w/v) NaCl and at temperatures ranging from 4 to 37°C. This isolate has catalase and oxidase activity and hydrolyses aesculin, DNA and l-tyrosine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain M1-2^T belongs to the genus Joostella and is clearly distinct from the other described species of this genus, Joostella marina (type strain En5^T). The 16S rRNA gene sequence similarity level between M1-2^T and J. marina En5^T is 97.2%, and the DNA–DNA relatedness value between the two strains is 23.9%. Strain M1-2^T contains MK-6 as the major menaquinone and iso-C_15:0, summed feature 3 (C_16:1 ω7c and/or iso-C_15:0 2OH) and iso-C_17:0 3OH as major cellular fatty acids. The DNA G + C content is 32.3 mol%. These data suggest that strain M1-2^T should be classified as a novel species, for which the name Joostella atrarenae sp. nov. is proposed. The type strain for the novel species is M1-2^T (= KCTC 23194^T = NCAIM B.002413^T).     

Fuscibacter oryzae gen. nov., sp. nov., a phosphate-solubilizing bacterium isolated from the rhizosphere of rice plant

An ovoid to rod shaped, white to brown pigmented, facultative anaerobic, mesophilic, non-phototrophic, Gram-staining-negative, non-motile, multiply by binary fission designated strain KVB23^T, which was isolated from root of rice plant, near Ilsan, South Korea, was investigated for its taxonomic position by polyphasic approach. Optimal growth was found to occur at 30?C, at pH 6.5 and in the absence of NaCl on R2A. Phylogenetic analysis based on the 16S rRNA gene sequence of strain KVB23^T revealed that it formed a distinct lineage, as a separate deep branch within the family Rhodobacteriaceae, with?<?96.5% sequence similarity to representatives of the genera Rhodobacter, Xinfangfangia, Tabrizicola, Falsirhodobacter, Haematobacter, Paenirhodobacter, Pseudorhodobacter and Pararhodobacter. Based in 16S rRNA sequences strain KVB23^T was most closely related to Tabrizicola fusiformis KCTC 62105 ^T (96.5%) and Rhodobacter thermarum KCTC 52712 ^T (96.2%). The draft genome of strain KVB23^T was 3.80 bp long with a DNA G?+?C content of 63.1%. Genome of strain KVB23^T harboured gene clusters for tryptophan and cobalamin biosynthesis. The strain contained Q-10 as the sole respiratory quinone. The predominant fatty acids were found to consist of C_16:0, C_18:0 and summed feature 8 (comprising C_18:1 ω7c and / or C_18:1 ω6). The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, seven unidentified phosphoglycolipids, two unidentified aminophosphoglycolipid, one unidentified glycolipid and four unidentified lipids. Phosphate-solubilizing bacteria have the ability to dissolve insoluble phosphates and enhance the soil fertility. Strain KVB23^T can solubilize calcium phosphate tribasic. Phosphate solubilizing and tryptophan biosynthesis property of strain KVB23^T could be a possible factor for the increase in growth of rice plant. Differential phenotypic, chemotaxonomic and genotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain KVB23^T was found to represent a novel genus in the Rhodobacteriaceae family, for which the name Fuscibacter oryzae gen. nov., sp. nov. is proposed, with the type strain KVB23^T(=?KACC 21711 ^T?=?NBRC 114716 ^T).

     

Pedobacter aquae sp. nov., a multi-drug resistant bacterium isolated from fresh water

A novel bacterial strain designated CJ43^T was isolated from fresh water located in Gangwon-do, South Korea, displaying multi-drug resistance. The isolate was Gram-stain-negative, aerobic, orange-pigmented, and rod-shaped. Strain CJ43^T grew optimally at 30 °C and pH 7 on R2A agar in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain CJ43^T belonged to the genus Pedobacter in the family Sphingobacteriaceae and was most closely related to Pedobacter puniceum HX-22-1 ^T and P. glucosidilyticus 1-2 ^T (98.3 and 98.1% sequence similarity). The genome size of strain CJ43^T was 3.9 Mb in a single contig with DNA G?+?C content of 34.9%. The genome included 3144 predicted protein-coding genes, as well as 55 tRNA, 9 rRNA and 3 ncRNA genes. The genome also contained 128 putative antibiotic resistance genes, reflecting its phenotypes. The average nucleotide identity values between strain CJ43^T and two closely related strains P. puniceum HX-22-1 ^T and P. glucosidilyticus 1-2 ^T were 91.0 and 88.7%, respectively. In silico digital DNA-DNA hybridization results between strain CJ43^T and the related strains were 42.8 and 38.6%, respectively. The major fatty acids of strain CJ43^T were iso-C_15:0, iso-C_17:0 3-OH, and summed feature 3 (C_16:1 ω6c and/or C_16:1 ω7c). Strain CJ43^T contained phosphatidylethanolamine as the major polar lipid and menaquinone-7 as the sole respiratory quinone. Based on the polyphasic taxonomy data, strain CJ43^T represents a novel species of the genus Pedobacter, for which the name Pedobacter aquae sp. nov. is proposed with the type strain CJ43^T (=?KACC 21350 ^T?=?JCM 33709 ^T).

     

Methylobacterium bullatum sp. nov., a methylotrophic bacterium isolated from Funaria hygrometrica

A novel, pink-pigmented aerobic, facultatively methylotrophic bacterial strain (F3.2^T) isolated from the phyllosphere of Funaria hygrometrica, was analyzed using a polyphasic approach. Cells were Gram-negative, motile rods, strictly aerobic and non-spore-forming and exhibited surface structures varying in quantity, distribution and morphology. The isolate grew at 10–33 °C over a pH range of 5.5–8.0 and in the presence of less than 1.0% NaCl. Strain F3.2^T shared less than 70% DNA–DNA binding to the next type strain of the genus Methylobacterium (M. adhaesivum DSM 17169^T). In addition to the major cellular fatty acid C_18:1ω7c (81.7%), present in all Methylobacterium species (and also members of the genus Alphaproteobacteria), a high value (11.7%) of the fatty acids (summed feature) C_16:1ω7c and/or iso-C_15:02OH was determined. Phylogenetic analyses based on 16S rDNA and methanol dehydrogenase gene sequences, DNA–DNA hybridization values, chemotaxonomic and phenotypic characteristics indicate that the strain F3.2^T represents a novel species within the genus Methylobacterium. We propose the name Methylobacterium bullatum sp. nov. for this species. The type strain is the strain F3.2^T (DSM 21893^T = LMG 24788^T).     

Nonhongiella spirulinensis gen. nov., sp. nov., a bacterium isolated from a cultivation pond of Spirulina platensis in Sanya, China

A Gram-negative, aerobic, motile rod strain, designated Ma-20^T, was isolated from a pool of marine Spirulina platensis cultivation, Sanya, China, and was subjected to a polyphasic taxonomy study. Strain Ma-20^T can grow in the presence of 0.5–11 % (w/v) NaCl, 10–43 °C and pH 6–10, and grew optimally at 30 °C, pH 7.5–9.0 in natural seawater medium. The polar lipids were composed of phosphatidylethanolamine, three unidentified phospholipids and three unidentified polar lipids. The respiratory quinone was ubiquinone 8 (Q-8) and the major fatty acids were C_18:1ω6c/C_18:1ω7c (summed feature 8, 32.84 %), C_16:1ω6c/C_16:1ω7c (summed feature 3, 30.76 %), C_16:0 (13.54 %), C_12:03-OH (4.63 %), and C_12:0 (4.09 %). The DNA G+C content of strain Ma-20^T was 58 mol %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Ma-20^T belonging to Gammaproteobacteria, it shared 88.46–91.55 and 89.21–91.26 % 16S rRNA gene sequence similarity to the type strains in genus Hahella and Marinobacter, respectively. In addition to the large 16S rRNA gene sequence difference, Ma-20^T can also be distinguished from the reference type strains Hahella ganghwensis FR1050^T and Marinobacter hydrocarbonoclasticus sp. 17^T by several phenotypic characteristics and chemotaxonomic properties. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain Ma-20^T is suggested to represent a novel species of a new genus in Gammaproteobacteria, for which the name Nonhongiella spirulinensis gen. nov., sp. nov. is proposed. The type strain is Ma-20^T (=KCTC 32221^T=LMG 27470^T).     

A genomic overview including polyphasic taxonomy of Thalassoroseus pseudoceratinae gen. nov., sp. nov. isolated from a marine sponge,Pseudoceratina sp.

A pink-coloured, salt- and alkali-tolerant planctomycetal strain (JC658^T) with oval to pear-shaped, motile, aerobic, Gram-negative stained cells was isolated from a marine sponge, Pseudoceratina sp. Strain JC658^T shares the highest 16S rRNA gene sequence identity with Maioricimonas rarisocia Mal4^T (<?89.2%) in the family Planctomycetaceae. The genomic analysis of the new strain indicates its biotechnological potential for the production of various industrially important enzymes, notably sulfatases and carbohydrate-active enzymes (CAZymes), and also potential antimicrobial compounds. Several genes encoding restriction-modification (RM) and CRISPR-CAS systems are also present. NaCl is obligate for growth, of which strain JC658^T can tolerate a concentration up to 6% (w/v). Optimum pH and temperature for growth are 8.0 (range 7.0–9.0) and 25 ºC (range 10–40 °C), respectively. The major respiratory quinone of strain JC658^T is MK6. Major fatty acids are C_16:1ω7c/C_16:1ω6c, C_18:0 and C_16:0. Major polar lipids are phosphatidylcholine, phosphatidyl-dimethylethanolamine and phosphatidyl-monomethylethanolamine. The genomic size of strain JC658^T is 7.36 Mb with a DNA G?+?C?content of 54.6 mol%. Based on phylogenetic, genomic (ANI, AAI, POCP, dDDH), chemotaxonomic, physiological and biochemical characteristics, we conclude that strain JC658^T belongs to a novel genus and constitutes a novel species within the family Planctomycetaceae, for which we propose the name Thalassoroseus pseudoceratinae gen. nov., sp. nov. The novel species is represented by the type strain JC658^T (=?KCTC 72881 ^T?=?NBRC 114371 ^T).

     

Deinococcus aestuarii sp. nov. and Deinococcus aquaedulcis sp. nov., two novel resistant bacteria isolated from pearl river estuary

Two novel species of the genus Deinococcus, designated SYSU M49105^T and SYSU M42101^T, were isolated from freshwater samples of the Pearl River estuary in Guangdong, China. Phylogenetic analysis using 16S rRNA gene sequence indicated that strains SYSU M49105^T and SYSU M42101^T showed the highest sequence similarities to Deinococcus aetherius JCM 11751 ^T (93.6%) and Deinococcus multiflagellatus NBRC 112888 ^T (97.3%), respectively. Cells of both strains were Gram-staining positive, aerobic, coccus-shaped, oxidase-negative and non-motile. The cell wall contained meso-diaminopimelic acid as their diagnostic diamino acid. MK-8 was the predominant respiratory quinone for both strains. The polar lipid profile of SYSU M49105^T contained two unidentified phosphoglycolipids, nine unidentified glycolipids, and five unidentified polar lipids. SYSU M42101^T had one unidentified phosphoglycolipid, nine unidentified glycolipids, one unidentified aminophospholipid and four unidentified polar lipids. The major fatty acids of strains SYSU M49105^T and SYSU M42101^T were summed feature 3 (C_16:1 ω7c and/ or C_16:1 ω6c) and C_16:0. The G?+?C contents of the novel isolates based on genomic DNAs were 69.6% and 67.4%, respectively. On the basis of phenotypic, genotypic and phylogenetic data, strains SYSU M49105^T and SYSU M42101^T should be considered to represent two novel species in the genus Deinococcus, for which the names Deinococcus aestuarii sp. nov. and Deinococcus aquaedulcis sp. nov. were proposed with the type strains SYSU M49105^T (=?KCTC 43258 ^T?=?CGMCC 1.18609 ^T) and SYSU M42101^T (=?KCTC 43257 ^T?=?CGMCC 1.18614 ^T), respectively.

     

<Emphasis Type="Italic">Paracoccus oceanense</Emphasis> sp. nov., Isolated from the West Pacific

A Gram-negative, short ovoid- to coccus-shaped, aerobic, motile, non-spore-forming bacterium (designated strain JLT1679^T) was isolated from West Pacific. Cells have subpolar flagella, dividing by binary fission. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belongs to branch of the evolutionary radiation occupied by the genus Paracoccus, family Rhodobacteraceae, order Rhodobacterales, class Alphaproteobacteria. The closest neighbours were Paracoccus stylophorae KTW-16^T (97.1% similarity), Paracoccus caeni strain MJ17^T (96.5% similarity), Paracoccus homiensis DD-R11^T (96.0% similarity) and Paracoccus alcaliphilus JCM 7364^T (95.8% similarity). The predominant cellular fatty acids of strain JLT1679^T were summed feature 8 (18:1ω6c) (38.8%), C_18:0 (27.7%), C_16:0 (22.5%), and significant amounts of C_18:1 ω9c (5.1%), C_14:0 (3.8%) and C_18:1 ω7c 11-methyl (2.1%), were present. The predominant respiratory ubiquinone of strain JLT1679^T was Q-10 and the DNA G + C content of strain JLT1679^T was 59.5 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. The isolate was distinguishable from members of the genus Paracoccus on the basis of phenotypic and biochemical characteristics. It is evident from the genotypic, chemotaxonomic and phenotypic data that strain JLT1679^T represents a novel species of the genus Paracoccus, for which the name Paracoccus oceanense sp. nov. is proposed. The type strain is JLT1679^T (= JCM 17768^T = CGMCC 1.10831^T).     

<Emphasis Type="Italic">Hoeflea prorocentri</Emphasis> sp. nov., isolated from a culture of the marine dinoflagellate <Emphasis Type="Italic">Prorocentrum mexicanum</Emphasis> PM01

A Gram-stain negative, aerobic, rod-shaped, non-motile, yellow-pigmented and non-spore-forming bacterial strain, designated PM5-8^T, was isolated from a culture of a marine toxigenic dinoflagellate Prorocentrum mexicanum PM01. Strain PM5-8^T grew at 15–35 °C (optimum, 25–30 °C) and pH 6–11 (optimum, 7.5–8). Cells required at least 1.5% (w/v) NaCl for growth, and can tolerate up to 7.0% with the optimum of 4%. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the strain PM5-8^T is closely related to members of the genus Hoeflea, with high sequence similarities with Hoeflea halophila JG120-1^T (97.06%) and Hoeflea alexandrii AM1V30^T (97.01%). DNA–DNA hybridization values between the isolate and other type strains of recognized species of the genus Hoeflea were between 11.8 and 25.2%, which is far below the value of 70% threshold for species delineation. The DNA G?+?C content was 50.3 mol%. The predominant cellular fatty acids of the strain were identified as summed feature 8 (C_16:1 ω7c and/or C_16:1 ω6c; 51.5%), C_18:1 ω7c 11-methyl (20.7%), C_16:0 (17.2%) and C_18:0 (5.7%). The major respiratory quinone was Q-10. Polar lipids profiles contained phosphatidylcholine, phosphatidylglycerol, sulfoquinovosyl diacylglycerol, phosphatidylmono- methylethanolamine, phosphatidylethanolamine and four unidentified lipids. On the basis of the polyphasic taxonomic data presented, strain PM5-8^T (=?CCTCC AB 2016294^T?=?KCTC 62490^T) represents a novel species of the genus Hoeflea, for which the name Hoeflea prorocentri sp. nov. is proposed.     

Rufibacter tibetensis gen. nov., sp. nov., a novel member of the family Cytophagaceae isolated from soil

A red-pigmented, Gram-negative, strictly aerobic, rod-shaped bacterium which was motile by gliding, designated strain 1351^T, was isolated from the soil of Lengduo, Tibet in China and subjected to a polyphasic taxonomic analysis. The isolate grows optimally at 30°C and pH 7. It grows with NaCl tolerated up to 1.5% (optimum, 0.5%). Phylogenetic analysis based on the 16S rRNA gene sequence shows that strain 1351^T is closely related to members of the family ‘Cytophagaceae’ closest sequence similarity was observed with similarity with Adhaeribacter terreus (91.8%). The major whole-cell fatty acids are summed feature 4 (containing anteiso-C_17:1 B and/or iso-C_17:1 I, 29.2%), summed feature 3 (containing C_16:1ω6c and/or C_16:1ω7c, 13.0%) and iso-C_15:0 (12.0%). The predominant menaquinone of strain 1351^T is menaquinone-7 (MK-7) and the G+C content of the DNA is 46.8 mol%. According to the phylogenetic evidence and phenotypic data, strain 1351^T is considered to represent a new genus and species of the family ‘Cytophagaceae’ for which the name Rufibacter tibetensis gen nov., sp. nov. is proposed. The type species is R. tibetensis and the type strain is 1351^T (=CCTCC AB 208084^T = NRRL B-51285^T).     

Caulobacter zeae sp. nov. and Caulobacter radicis sp. nov., novel endophytic bacteria isolated from maize root (Zea mays L.)

Four bacterial strains designated 410^T, 441, 695^T and 736 were isolated from maize root in Beijing, P. R. China. Based on 16S rRNA gene phylogeny, the four strains formed two clusters in the genus Caulobacter. Since strain 441 was a clonal variety of strain 410^T, only three strains were selected for further taxonomic studies. The whole genome average nucleotide identity (ANI) value between strains 410^T and 695^T was 94.65%, and both strains shared less than 92.10% ANI values with their close phylogenetic neighbors Caulobacter vibrioides DSM 9893^T, Caulobacter segnis ATCC 21756^T and Caulobacter flavus CGMCC 1.15093^T. Strains 410^T and 695^T contained Q-10 as the sole ubiquinone and their major fatty acids were C_{16:0, 11-methyl C18:1ω 0}, 11-methyl C_{18: 1}ω7c, summed feature 3 (C_{16:1ω7c and/or C16:1ω 1}ω7c and/or C_{16: 1}ω6c) and summed feature 8 (C_{18:1ω7c and/or C18:1ω 1}ω7c and/or C_{18: 1}ω6c). Their major polar lipids consisted of glycolipids and phosphatidylglycerol, and phenotypic tests differentiated them from their closest phylogenetic neighbors. Based on the results obtained, it is proposed that the three strains represent two novel species, for which the names Caulobacter zeae sp. nov. (type strain 410^T = CGMCC 1.15991 = DSM 104304) and Caulobacter radicis sp. nov. (type strain 695^T = CGMCC 1.16556 = DSM 106792) are proposed.     

<Emphasis Type="Italic">Sphingomonas jejuensis</Emphasis> sp. nov., isolated from marine sponge <Emphasis Type="Italic">Hymeniacidon flavia</Emphasis>

A Gram-negative, non-motile, rod shaped, and orange-pigmented chemoheterotrophic bacterium, strain MS-31^T was isolated from the marine sponge Hymeniacidon flavia, collected from near Jeju Island, Korea. The Strain MS-31^T was subjected to a polyphasic taxonomic study. The phylogenetic analysis based on the 16S rRNA gene sequences revealed that the novel isolate could be affiliated within the genus Sphingomonas. The strain MS-31^T showed 95.6% of 16S rRNA gene sequence similarity with the most closely related species Sphingomonas koreensis JSS26^T. The DNA G+C content of the strain MS-31^T was 69.4 mol%. The major isoprenoid quinone was ubiqunone 10 and predominant cellular fatty acids were summed feature 7 (comprising C_18:1 ω7c, C_18:1 Ω9t and/or C_18:1 ωl2t, 39.7%), C_16:0 (16.3%), C_14:0 2OH (15.9%) and summed feature 3 (comprising C_16:1 ω7c and/or C_15:0 iso 2OH, 11.7%). The polar lipids were sphingoglycolipid, phosphatidyletha-nolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified glycolipid. Based on the evidence from the polyphasic taxonomic study, the strain should be classified as a new species of the genus Sphingomonas. As a result, the name Sphingomonas jejuensis sp. nov. (type strain MS-31^T =KCTC 23321^T =NBRC 107775^T) is proposed.