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1.
A novel actinomycete, designated strain KLBMP 4601 T, was isolated from the root of the medicinal plant Curcuma phaeocaulis collected from Sichuan Province, south-west China. The strain produced extensively branched substrate and aerial hyphae that carried straight to flexuous spore chains. Chemotaxonomic properties of this strain were consistent with those of members of the genus Streptomyces. The cell wall of strain KLBMP 4601 T contained ll-diaminopimelic acid as the characteristic diamino acid. The major menaquinone was MK-9(H 4), with minor amounts of MK-9(H 6), MK-9(H 8) and MK-10(H 2). The major fatty acids were C 16:0, iso-C 16:0, C 18:1ω9 c and C 16:1, iso G. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KLBMP 4601 T belongs to the genus Streptomyces and is most closely related to Streptomyces armeniacus JCM 3070 T (97.9 %), Streptomyces pharmamarensis PM267 T (97.6 %) and Streptomyces artemisiae YIM 63135 T (97.5 %). The 16S rRNA gene sequence similarity between strain KLBMP 4601 T and other members of this genus were lower than 97.5 %. DNA–DNA hybridization studies of strain KLBMP 4601 T with the three closest species showed relatedness values of 36.3 ± 4.2 %, 27.3 ± 0.6 %, and 30.9 ± 2.5 %, respectively. On the basis of chemotaxonomic, phenotypic and genotypic characteristics, it is evident that strain KLBMP 4601 T represents a novel species of the genus Streptomyces, for which the name Streptomyces phytohabitans sp. nov. is proposed. The type strain is KLBMP 4601 T (=KCTC 19892 T = NBRC 108772 T). 相似文献
2.
A novel actinomycete strain, GW25-5 T, was isolated from a soil sample collected from the Fildes Peninsula, King George Island, West Antarctica. The strain was
characterized by white to grey aerial mycelia, which were differentiated to straight to flexuous spore chains, with rod-shaped
smooth spores. The cell wall of strain GW25-5 T contained LL-diaminopimelic acid (A 2pm) and traces of meso-A 2pm. Whole-cell sugars were galactose and minor amounts of mannose and glucose. The predominant menaquinones were MK-9(H 6) (49%), MK-9(H 8) (24%) and MK-9(H 4) (12%). The phospholipids contained DPG, PE, PI, PIM and PL(s). The major cellular fatty acids were iso-C 16:0 and anteiso-C 15:0. Genomic DNA G+C content of strain GW25-5 T was 70.0 mol%. BLAST result showed that strain GW25-5 has the 16S rRNA gene sequence highest similarity of 97.5% with members
of genus Streptomyces and phylogenetic analysis indicated that this strain belongs to the genus Streptomyces. DNA–DNA relatedness values of strain GW25-5 T with the closest species of Streptomyces
purpureus LMG 19368 T and Streptomyces
beijiangensis YIM 6 T were significantly lower than 70% of the threshold value for the delineation of genomic species. A polyphasic taxonomic investigation
based on a judicious combination of genotypic and phenotypic characteristics revealed that the organism represents a novel
species of the genus Streptomyces. Thus, we propose strain GW25-5 T as the type strain of this novel species, Streptomyces
fildesensis (=CGMCC 4.5735 T = YIM 93602 T = DSM 41987 T = NRRL B 24828 T). 相似文献
3.
During an investigation exploring potential sources of novel thermophilic species and natural products, a novel thermophilic and alkaliphilic actinomycete with alkaline cellulase producing ability, designated strain 4-2-13 T, was isolated from soil of a tropical rainforest in Xishuangbanna, Yunnan province, China. The morphological and chemotaxonomic characteristics of strain 4-2-13 T are consistent with those of the members of the genus Streptomyces. The strain forms extensively branched aerial mycelia and substrate mycelia. Spiral spore chains were observed on aerial mycelia; spores were oval to cylindrical, with smooth surfaces. The organism was found to contain ll-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan. The whole cell hydrolysates were found to contain glucose and ribose. The cellular fatty acid profile mainly consists of anteiso-C 17:0 and iso-C 16:0. The menaquinones were identified as MK-9(H 8), MK-10(H 6) and MK-9(H 6). The polar lipids profile were found to consist of diphosphatidylglycerol, phosphatidylmethylethanolamine, a ninhydrin-positive glycophospholipid, phosphatidylinositol, phosphatidylglycerol and unidentified glycolipids. The 16S rRNA gene sequence analysis showed that the organism belongs to the genus Streptomyces and in the 16S rRNA gene tree it formed a distinct phyletic line together with the closely related type strain Streptomyces burgazadensis Z1R7 T (95.2% sequence similarity). However, the phenotypic characteristics of strain 4-2-13 T are significantly different from those of S. burgazadensis Z1R7 T. Based on the phenotypic, chemotaxonomic and phylogenetic characteristics, strain 4-2-13 T represents a novel species in the genus Streptomyces, for which the name Streptomyces thermoalkaliphilus sp. nov. is proposed. The type strain is 4-2-13 T (= DSM 42159 T = CGMCC 4. 7205 T). 相似文献
4.
An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110T, was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110T was a member of the genus Streptomyces and showed the closest similarities to Streptomyces buecherae AC541T (99.2%), followed by Streptomyces rapamycinicus NRRL B-5491T (99.1%), Streptomyces luteoverticillatus NBRC 3840T (99.1%), Streptomyces coerulescens NBRC 12758T (99.1%), and Streptomyces iranensis HM 35T (99.0%). Strain CA3R110T contained LL-diaminopimelic acid in cell peptidoglycan, MK-9(H6), and MK-9(H8) as major menaquinone, iso-C16:0, iso-C15:0, C16:0 as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus Streptomyces. A low digital DNA–DNA hybridization (<?55.7%) and average nucleotide identity-blast (ANIb) (<?92.2%) values revealed that strain CA3R110T could be distinguished from any known Streptomyces species. With the differences in phenotypic and genotypic data, strain CA3R110T represents a novel species of genus Streptomyces, for which the name Streptomyces endocoffeicus sp. nov. is proposed. The type strain is CA3R110T (=?TBRC 11245T?=?NBRC 114296T). 相似文献
5.
A total of 37 actinobacteria were isolated from eighteen lichen samples collected in Thailand. Based on the 16S rRNA gene sequences, they were identified into five genera including Actinoplanes (1 strain), Actinomadura (1 strain), Pseudosporangium (1 strain), Wangella (1 strain) and Streptomyces (33 strains). Among these isolates, strain Ptm05T, Ptm01 and Ptm12 showed low 16S rRNA gene similarity and was selected for the further taxonomic study using the polyphasic approach. These strains showed the highest 16S rRNA gene sequence similarity with Streptomyces sparsogenes ATCC 25498T (97.44–97.72%). Strain Ptm05T was selected for the type strain. The chemical cell composition of the strain was similar to the members of Streptomyces genus. LL-diaminopimelic acids were detected in the peptidoglycan. Menaquinones were MK-9(H8) and MK-9(H6). Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, one unidentified phospholipid, one unidentified glycolipid and one unidentified lipid were detected as the polar lipids. The predominant cellular fatty acids are anteiso-C15:0, iso-C15:0, iso-C16:0, iso-C17:0 and C16:0. The dDNA-DNA hybridization values among strain Ptm05T and its closely related Streptomyces type strains were 17.2–18.0%. In addition, the ANIb and ANIm between strain Ptm05T and related Streptomyces type strains were ranged from 75.69 to 76.13% and 85.21 to 85.35%, respectively. Based on phenotypic and genomic evidence, strain Ptm05T (=?TBRC 14546T?=?NBRC 115203T) represents the novel species of the genus Streptomyces for which the name Streptomyces parmotrematis sp. nov. is proposed. This study showed that the lichens are the promising source of the novel actinobacterial taxa. 相似文献
6.
During an investigation exploring potential sources of novel species and natural products, a novel actinomycete with antifungal activity, designated strain NEAU-Gz11 T, was isolated from a soil sample, which was collected from Gama, Chad. The isolate was found to have morphological and chemotaxonomic characteristics typical of members of the genus Streptomyces. 16S rRNA gene sequence similarity studies showed that strain NEAU-Gz11 T belongs to the genus Streptomyces with high sequence similarity to Streptomyces hiroshimensis JCM 4098 T (98.0 %). Similarities to other type strains of the genus Streptomyces were lower than 98.0 %. However, the physiological and biochemical characteristics and low levels of DNA–DNA relatedness could differentiate the isolate genotypically and phenotypically from S. hiroshimensis JCM 4098 T. Therefore, the strain is concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces gamaensis sp. nov. is proposed. The type strain is NEAU-Gz11 T (=CGMCC 4.7304 T=DSM 101531 T). 相似文献
7.
A novel Gram-stain positive, spore-forming, aerobic actinomycete, designated strain NEAU-QTH3-11 T, was isolated from muddy soil collected from a stream in Qitaihe, Heilongjiang Province, northeast China and characterised using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-QTH3-11 T belongs to the genus Streptomyces and is closely related to Streptomyces rhizosphaerihabitans NBRC 109807 T (99.38%) and Streptomyces mirabilis JCM 4791 T (99.03%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain formed a cluster with S. rhizosphaerihabitans NBRC 109807 T and Streptomyces siamensis NBRC 108799 T (98.62%). The menaquinones were identified as MK-9(H 8), MK-9(H 6) and MK-9(H 4). The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as anteiso-C 15:0, iso-C 16:0, C 16:0 and C 15:0. However, multilocus sequence analysis based on five house-keeping genes ( atpD, gyrB, rpoB, recA and trpB), low DNA-DNA hybridization results and some phenotypic, physiological and biochemical properties could differentiate the strain from its close relatives in the genus Streptomyces. Therefore, strain NEAU-QTH3-11 T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces lutosisoli sp. nov. is proposed, with NEAU-QTH3-11 T (=DSM 42165 T=CGMCC 4.7198 T) as the type strain. 相似文献
8.
An actinomycete strain, designated strain LUSFXJ T, was isolated from a soil sample obtained near the Xiangtan Manganese Mine, Central-South China and characterised using a polyphasic taxonomic approach. The 16S rRNA gene sequence-based phylogenetic analysis indicated that this strain belongs to the genus Streptomyces. The DNA–DNA relatedness between this strain and two closely related type strains, Streptomyces echinatus CGMCC 4.1642 T and Streptomyces lanatus CGMCC 4.137 T, were 28.7 ± 0.4 and 19.9 ± 2.0%, respectively, values which are far lower than the 70% threshold for the delineation of a novel prokaryotic species. The DNA G+C content of strain LUSFXJ T is 75.0 mol%. Chemotaxonomic analysis revealed that the menaquinones of strain LUSFXJ T are MK-9(H 6), MK-9(H 8), MK-9(H 2) and MK-8(H 8). The polar lipid profile of strain LUSFXJ T was found to contain diphosphatidylglycerol and an unidentified polar lipid. The major cellular fatty acids were identified as iso-C 15:0, anteiso-C 15:0, iso-C 16:0, C 16:0 and Summed feature 3. Strain LUSFXJ T was found to contain meso-diaminopimelic acid as the diagnostic cell wall diamino acid and the whole cell hydrolysates were found to be rich in ribose, mannose and glucose. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, it is concluded that strain LUSFXJ T represents a novel species of the genus Streptomyces, for which the name S. xiangtanensis sp. nov. is proposed. The type strain is LUSFXJ T (=GDMCC 4.133 T = KCTC 39829 T). 相似文献
9.
A novel actinomycete strain YIM 31724 T was isolated from a soil sample collected from Dali, Yunnan Province, People’s Republic of China. The strain is characterized
by white to yellow white aerial mycelia, spiral spore chains and smooth spore surface. The cell wall of strain YIM 31724 T contained LL-diaminopimelic acid (A 2pm) and traces of meso-A 2pm. Whole-cell hydrolysates contained mainly glucose and small amounts of galactose and xylose. The menaquinones were MK-9(H 6) (31%) and MK-9(H 8) (69%). Phosphatidylethanolamine was the diagnostic phospholipid. The DNA G+C content of strain YIM 31724 T was 67.2 mol%. Phylogenetic analysis indicated that the strain belongs to the genus Streptomyces, with highest similarity to Streptomyces rimosus subsp. rimosus JCM 4667 T (rRNA gene sequence similarity value of 98.9%) and Streptomyces erumpens DSM 40941 T (rRNA gene sequence similarity value of 98.7%). Based on its phenotypic and genotypic characteristics, including low DNA–DNA
hybridization results, strain
is proposed as the type strain of a novel species, Streptomyces daliensis sp. nov. 相似文献
10.
A Streptomyces-like actinomycete strain, designated as YIM 78087 T, was isolated from a sediment sample collected from Hehua hot spring in Tengchong, Yunnan province, south-west China. The taxonomic position of strain YIM 78087 T was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain YIM 78087 T belongs to the genus Streptomyces and is closely related to Streptomyces fimbriatus DSM 40942 T, Streptomyces marinus DSM 41968 T and Streptomyces qinglanensis DSM 42035 T (97.18, 97.05 and 97.1 % similarity, respectively). Combined with the low values of DNA–DNA hybridization between strain YIM 78087 T and its closest neighbours, these analyses indicated that this new isolate represents a different genomic species in the genus Streptomyces. The predominant menaquinones of strain YIM 78087 T were identified as MK-9 (H 4) and MK-9 (H 6). The major fatty acids were identified as anteiso-C 15:0 (28.4 %), anteiso-C 17:0 (23.0 %) and iso-C 16:0 (15.1 %). The whole-cell hydrolysates found to contain glucose, mannose and ribose. The DNA G+C content was determined to be 73.0 mol%. Based on the comparative analysis of phenotypic and genotypic characteristics, it is proposed that strain YIM 78087 T represents a novel species of the genus Streptomyces, for which the name Streptomyces calidiresistens sp. nov., is proposed. The type strain is YIM 78087 T (=BCRC 16955 T=DSM 42108 T=JCM 19629 T). 相似文献
11.
The taxonomic position of a Streptomyces strain isolated from a hyper-arid desert soil was established using a polyphasic approach. The organism had chemical and
morphological properties typical of the genus Streptomyces and formed a phyletic line at the periphery of the Streptomyces coeruleorubidus subcluster in the 16S rRNA gene tree. DNA:DNA relatedness values between the isolate and its nearest phylogenetic neighbours,
Streptomyces lomondensis NRRL 3252 T and Streptomyces lusitanus NRRL B-12501 T were 42.5 (±0.48)% and 25.0 (±1.78)%, respectively. The isolate was readily distinguished from these organisms using a combination
of morphological and phenotypic properties. On the basis of these results, it is proposed that isolate C63 T (CGMCC 4.6997 T, = KACC 15425 T) be classified as the type strain of Streptomyces deserti sp. nov. 相似文献
12.
During a screening for novel and useful actinobacteria in desert animal, a new actinomycete was isolated and designated strain TRM63209T. The strain was isolated from in vivo of a Blattella germanica in Tarim University in Alar City, Xinjiang, north-west China. The strain was found to exhibit an inhibitory effect on biofilm formation by Candida albicans ATCC 18,804. The strain was observed to form abundant aerial mycelium, occasionally twisted and which differentiated into spiral spore chains. Spores of TRM63209T were observed to be oval-shaped, with a smooth surface. Strain TRM63209T was found to grow optimally at 28 °C, pH 8 and in the presence of 1% (w/v) NaCl. The whole-cell sugars of strain TRM63209T were rhamnose ribose, xylose, mannose, galactose and glucose, and the principal polarlipids were found to be diphosphatidylglycerol, phos-phatidylethanolamine, phosphatidylcholine, phosphatidylinositol mannoside, phosphatidylinositol and an unknown phospholipid(L). The diagnostic cell wall amino acid was identified as LL-diaminopimelic acid. The predominant menaquinone was found to be MK-9(H6) (14.64%), MK-9(H2) (19.65%), MK-9(H8) (22.34%), MK-10(H2) (25.37%). The major cellular fatty acids were identified as iso-C16:0, 16:0, anteiso-C15:0, anteiso-C17:0, iso-C15:0 and Sum in Feature 3. Analysis of the 16S rRNA sequence showed that strain TRM63209T exhibits high sequence similarity to Streptomyces bungoensis strain DSM 41781T 98.20%. A multi-locus sequence analysis of five house-keeping genes (atpD, gyrB, rpoB, recA and trpB) and phylogenomic analysis also illustrated that strain TRM63209T should be assigned to the genus Streptomyces. The DNA G?+?C content of the strain was determined to be 70.2 mol%. Average nucleotide identity (ANI) between strain TRM63209T and S. bungoensis DSM 41781T, Streptomyces phyllanthi PA1-07T, Streptomyces longwoodensis DSM 41677T and Streptomyces caeruleatus NRRL B-24802T were 82.76%, 82.54%, 82.65%, 84.02%, respectively. Digtal DNA-DNA (dDDH) hybridization were 26.30%, 25.10%, 26.20%, 29.50%, respectively. Therefore, it is concluded that strain TRM63209T represents a novel species of the genus Streptomyces, for which the name Streptomyces blattelae is proposed. The type strain is TRM63209T (CCTCC AA 2018093T?=?LMG 31,403?=?TRM63209T). 相似文献
13.
A novel bacterium capable of fixing nitrogen was isolated from plantain rhizosphere soil in China. The isolate, designated YN-83 T, is Gram-positive, aerobic, motile and rod-shaped (0.4–0.6 μm × 1.9–2.6 μm). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain YN-83 T was a member of the genus Cohnella. High similarity of 16S rRNA gene sequence was found between YN-83 T and Cohnella ginsengisoli DSM18997 T (97.99%), whereas the similarity was below 96.0% between YN-83 T and the other Cohnella species. DNA–DNA relatedness between strain YN-83 T and C. ginsengisoli DSM18997 T was 27.4 ± 6.2%. The DNA G+C content of strain YN-83 T was 59.3 mol%. The predominant isoprenoid quinone was MK-7 and the major fatty acids were anteiso-C 15:0 (44.3%), iso-C 15:0 (11.3%), iso-C 16:0 (18.6%) and C 16:0 (7.7%). The polar lipids of strain YN-83 T consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lyso- phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. On the basis of phenotypic and chemotaxonomic properties, 16S rRNA gene sequence, G+C content and DNA–DNA hybridization, strain YN-83 T represents a novel species of the genus Cohnella, for which the name Cohnella plantaginis sp. nov. (type strain YN-83 T = DSM 25424 T = CGMCC 1.12047 T) is proposed. 相似文献
14.
A novel alkaliphilic and moderate halophilic bacterium, designated strain K164 T, was isolated from Keke Salt Lake in Qinghai, China. The strain grew with 2.0–20.0% (w/v) NaCl, at 4–50°C and pH 6.5–11.5,
with an optimum of 8% (w/v) NaCl, 37°C and pH 10, respectively. The predominant respiratory quinone was menaquinone 6 (MK-6)
and the major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were anteiso-C 15:0 and iso-C 15:0. The genomic DNA G+C content was 50.16 mol. Phylogenetic analysis based on the full-length 16S rRNA gene sequence revealed
that strain K164 T was a member of the genus Salinicoccus. Strain K164 T showed the highest similarity (98.4%) with Salinicoccus alkaliphilus AS 1.2691 T and below 97% similarity with other recognized members of the genus in 16S rRNA gene sequence. Level of DNA–DNA relatedness
between strain K164 T and Salinicoccus alkaliphilus AS 1.2691 T was 20.1%. On the basis of its phenotypic characteristics and the level of DNA–DNA hybridization, strain K164 T is considered to represent a novel species of the genus Salinicoccus, for which the name Salinicoccus kekensis sp. nov. is proposed. The type strain is K164 T (=CGMCC 1.10337 T = DSM 23173 T). 相似文献
15.
A novel actinomycete strain, designated YIM 45720 T, was isolated from a Cephalotaxus fortunei rhizophere soil sample collected from Yunnan Province, southwest China. The strain formed well-differentiated aerial and
substrate mycelia. Chemotaxonomically, it contained LL-diaminopimelic acid in the cell wall. The cell-wall sugars contained
ribose, mannose, and galactose with traces of glucose and xylose. Phospholipids were diphosphatidylglycerol, phosphatidylethanolamine,
phosphatidylcholine and phosphatidylinositol. MK-9 (H 8) was the predominant menaquinone. The major fatty acids (>10%) were iso-C 16:0, iso-C 15:1 and anteiso-C 15:0. The G + C content of the DNA was 70 mol%. Phylogenetic analysis data based on 16S rRNA gene sequence showed that strain YIM 45720 T formed a distinct branch with the type strain of Streptomyces scabrisporus JCM 11712 T within the genus Streptomyces. On the basis of the phenotypic and genotypic characteristics, strain YIM 45720 T (=DSM 41883 T = CCTCC AA 206006 T) is proposed as the type strain of a novel species, Streptomyces serianimatus sp. nov. 相似文献
16.
An actinomycete strain 232617 T was isolated from a composite mangrove sediment sample collected in Haikou, China. Phylogenetic analysis of the 16S rRNA
gene sequence of strain 232617 T indicated the highest similarity with Micromonospora siamensis TT2-4 T (99.05%), Micromonospora krabiensis A-2 T (98.99%) and Micromonospora carbonacea DSM 43815 T (98.91%). The gyrB gene sequence analysis also indicated that 232617 T should be assigned to the genus Micromonospora. The cell wall contains meso-DAP and glycine. The major menaquinones were MK-10(H 4) and MK-10(H 6), with MK-9(H 4) as minor components. The characteristic whole-cell sugars are xylose, arabinose and glucose. The phospholipid profile comprises
phosphatidylethanolamine, diphosphatidlglycerol and phosphatidylinositol mannoside. The DNA G+C content is 71.5 mol%. Furthermore,
a combination of DNA–DNA relatedness and some physiological and biochemical properties indicated that the novel strain could
be readily distinguished from the closest related species. On the basis of these phenotypic and genotypic data, strain 232617 T represents a novel species of the genus Micromonospora, for which the name Micromonospora haikouensis sp. nov. is proposed. The type strain is 232617 T (= CCTCC AA 201112 T = DSM 45626 T). 相似文献
17.
A novel endophytic actinomycete strain, designated KM-1-2 T, was isolated from seeds of Ginkgo biloba at Yangling, China. A polyphasic approach was used to study the taxonomy of strain KM-1-2 T and it was found to show a range of phylogenetic and chemotaxonomic properties consistent with those of members of the genus Streptomyces. The diamino acid of the cell wall peptidoglycan was identified as LL-diaminopimelic acid. No diagnostic sugars were detected in whole cell hydrolysates. The predominant menaquinones were identified as MK-9(H 6) and MK-9(H 8). The diagnostic phospholipids were found to be phosphatidylethanolamine and phosphatidylcholine. The DNA G + C content of the novel strain was determined to be 72.9 mol%. The predominant cellular fatty acids (> 10.0?%) were identified as iso-C 14?:?0, iso-C 16?:?0, C 16?:?0 and C 17?:?0 cyclo. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain is closely related to Streptomyces carpaticus JCM 6915 T (99.3%), Streptomyces harbinensis DSM 42076 T (98.9%) and Streptomyces cheonanensis JCM 14549 T (98.5%). DNA-DNA hybridizations with these three close relatives gave similarity values of 39.1 ± 1.9, 35.8 ± 2.3, and 47.4 ± 2.7%, respectively, which indicated that strain KM-1-2 T represents a novel species of the genus Streptomyces. This is consistent with the morphological, physiological and chemotaxonomic data. Cumulatively, these data suggest that strain KM-1-2 T represents a novel Streptomyces species, for which the name Streptomyces ginkgonis sp. nov. is proposed, with the type strain KM-1-2 T (= CCTCC AA2016004 T = KCTC 39801 T). 相似文献
18.
A Streptomyces strain isolated from a hyper-arid Atacama Desert soil was characterised using a polyphasic taxonomic approach. The strain, designated C2 T, had chemical and morphological properties typical of the genus Streptomyces. The isolate formed a branch in the Streptomyces 16S rRNA gene tree together with the type strain of Streptomyces chromofuscus and was also loosely related to Streptomyces fragilis NRRL 2424 T. DNA:DNA relatedness values between the isolate and its two phylogenetic neighbours showed that it formed a distinct genomic species. The strain was readily distinguished from these organisms using a combination of morphological and phenotypic data. Based on the genotypic and phenotypic results, isolate C2 T represents a novel species in the genus Streptomyces, for which the name Streptomyces bullii sp. nov. is proposed. The type strain is C2 T (=CGMCC 4.7019 T = KACC 15426 T). 相似文献
19.
A novel actinobacterial strain, designated TRM SA0054 T, was isolated from the roots of Sophora alopecuroides grown in Alar, Xinjiang, north-west China, and characterised by a polyphasic taxonomic approach. Phylogenetic analysis showed that strain TRM SA0054 T has 16S rRNA gene sequence similarity of 98.22% with Streptomyces barkulensis RC 1831 T. Whole cell hydrolysates of strain TRM SA0054 T were found to contain ll-diaminopimelic acid as the diagnostic diamino acid and ribose, glucose and xylose as the major whole cell sugars. The major fatty acids were identified as iso-C 16:0, iso-C 16:1 G, anteiso-C 17:0, anteiso-C 15:0, C 16:0, anteiso-C 17:1 ω9c. The main menaquinones were determined to be MK-8 (H 4), MK-9 (H 6) and MK-9 (H 8). The polar lipids were identified as diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified lipid. The G?+?C content of the genomic DNA was determined to be 73.04%. Strain TRM SA0054 T has a relatively low DNA–DNA relatedness value with Streptomyces barkulensis RC 1831 T as determined by calculating the average nucleotide identity value (ANI?=?84.1%). Based on the phenotypic, chemotaxonomic and phylogenetic data, it is concluded that strain TRM SA0054 T should be designated as a novel species of the genus Streptomyces, for which the name Streptomyces carminius sp. nov. is proposed, with type strain TRM SA0054 T (=?CCTCC AA 2016041 T?=?KCTC39903 T). 相似文献
20.
An endophytic actinomycete strain, designated Hhs.015 T, was isolated from roots of cucumber seedlings. The endophytic isolate was identified by means of a polyphasic taxonomic
approach. On the basis of 16S rRNA gene sequence similarities, strain Hhs.015 T was closely related to members of the genus Saccharothrix. DNA–DNA hybridization with the four closest relatives, Saccharothrix longispora NRRL B-16116 T, Saccharothrix xinjiangensis NRRL B-24321 T, Saccharothrix
autraliensis CGMCC 4.1355 T and Saccharothrix espanaensis CGMCC 4.1714 T, gave similarity values of 33.8, 28.2, 44.1 and 29.5%, respectively, which indicated that strain Hhs.015 T represents a novel species of the genus Saccharothrix. This is consistent with the morphological, physiological and chemotaxonomic data. As a whole, these results suggest that
strain Hhs.015 T represents a novel Saccharothrix species. The name Saccharothrix yanglingensis sp. nov. is proposed, with the type strain Hhs.015 T (=CGMCC 4.5627 T = KCTC 19722 T). 相似文献
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