Relationship between intake of tannin-containing tropical tree forage,PEG supplementation,and salivary haze development in hair sheep and goats

The objective of this study was to estimate the relationship between tannin binding salivary protein (TBSP) and condensed tannins (CT) intake in hair sheep and creole goats. Foliage was obtained from trees with different levels of CT content; animals were offered foliage ad libitum, with or without polyethylene glycol (PEG). Saliva haze development (SHD) was evaluated as evidence for TBSP. PEG consumption did not affect dry matter intake (DMI) (P > 0.05). Lignin (R = −0.714, P < 0.001) and Crude Protein (CP) (R = 0.622, P < 0.001) contents had a stronger association with DMI than CT (R = 0.622, P < 0.011) in sheep; no significant association was found in goats. The positive relationship between tannin intake and SHD (P < 0.05) was not confirmed after PEG supplementation in sheep (P > 0.09), but remained significant for goats (P < 0.01), except for those fed Lysiloma latisiliquum (P = 0.07). Foliage lignin or CP contents are better predictors of foliage intake than CT. Sheep and goats fed with tropical tree forages containing different levels of tannins exhibited differences in intake behavior; moreover, individual variations in TBSP expression helps explaining foliage DMI.     

Effects of Brosimum alicastrum and Lysiloma latisiliquum mixtures on voluntary intake, nutrient digestibility and nitrogen balance in sheep fed tropical pastures

Effects of supplementing tree foliage mixtures on voluntary intake, apparent digestibility and N balance was evaluated using Pelibuey sheep fed low quality diets. Five treatments were examined in a 5 × 5 Latin square design, which consisted of a basal diet of grass (Sorghum halepense) hay supplemented with Brosimum alicastrum (B) and Lysiloma latisiliquum (L) at the following rates (g DM/kg diet): B264, L0; B198, L66; B132, L132; B66, L198 and B0, L264. Additionally, an in situ degradability evaluation was completed with two ruminally cannulated cows. Neutral detergent fibre (NDF), acid detergent insoluble N (ADIN), lignin(sa) and total phenols (TP) were higher (P<0.01) in L. latisiliquum versus B. alicastrum. Daily intake (g/kg LW^0.75/day) of DM (from 98 to 73) and OM (from 88 to 66) decreased quadratically (P<0.01), whereas CP (from 8.0 to 5.6) and ME (from 7.7 to 5.2, MJ/sheep/day) reduced linearly (P<0.01), as L. latisiliquum increased in the diet. Apparent digestibility of DM (from 0.486 to 0.445), OM (from 0.511 to 0.458) and CP (from 0.417 to 0.198) decreased linearly (P<0.01) and was associated with a low ruminal in situ CP degradability of L. latisiliquum. The decrease in N intake and digestibility induced lower (P<0.01) N retention (from 2.7 to 0.1 g/sheep/day). Although the incremental substitution of B. alicastrum with L. latisiliquum negatively affected intake, rumen degradation, digestibility and N balance, results indicate that this foliage mixture, but with no more than 132 g DM/kg diet of L. latisiliquum, could be used as a supplementation strategy to sheep fed low quality forage without negative effects on voluntary intake.     

Tannins in tropical tree fodders fed to small ruminants: A friendly foe?

Livestock production systems worldwide rely largely on conventional feedstuffs. The current world food crisis highlights the need to improve the use of local resources for animal nutrition, such as fodder trees and shrubs. The detrimental effects of tropical tannin-rich plants (TRP) on animal production have been frequently described. In contrast, their potential benefits have long been neglected. This paper presents the potential positive effects of tropical TRP on small ruminants either as source of feed or as nutraceuticals with anthelmintic (AH) properties. It also analyses the host behavioral and physiological adaptations associated with exploitation of those tannin-rich resources. Both sheep and goats preferred a mixture of plants even when tannin-free forage was available. Moreover, the preference for TRP by goats and hair sheep were mainly associated with the digestible fraction of fiber and to a less extent with tannin content, which implies that they do not necessarily select against TRP. The addition of polyethylene glycol did not modify the preference or intake of TRP by goats and sheep. Evidence of physiological adaptation to TRP is presented and discussed. Both, experienced hair sheep and goats had saliva with tannin binding capacity, enabling both species to eat higher quantities of TRP which could lead to a higher availability of tannins in the gastrointestinal tract. Tannins in the gastrointestinal tract could be an AH against gastrointestinal nematodes (GIN). Indeed, in vitro and in vivo studies have shown AH effects of tannins from TRP, suggesting their possible use as natural anthelmintics against GIN. This paper supports the change in the current view of tannins in TRP as anti-nutritional compounds. If adequately managed, TRP can be a valuable component of sustainable small ruminant production systems.     

Responses of hair and wool sheep to a single fixed dose of infective larvae of Haemonchus contortus

Body weights, fecal egg counts (FEC), and packed cell volumes (PCV) of hair sheep and wool sheep crossbred lambs were compared over 8 weeks following administration of a single dose of approximately 10,000 third-stage larvae of Haemonchus contortus. Hair sheep lambs (n=17) were reciprocal crosses between mainland USA populations of Barbados Blackbelly (BB) and Virgin Islands White (VIW) sheep. Wool sheep lambs (n=64) were from a crossbred composite of 50% Dorset, 25% Rambouillet, and 25% Finnish Landrace breeding. Lambs of both breed types continued to grow during the period of infection. Mean weights were higher for wool lambs (39.7±0.8 kg) than for hair lambs (28.2±1.5 kg). FEC increased to week 5 in both breed groups and remained elevated in wool lambs through week 7 but declined sharply in hair lambs after week 5. Mean FEC for weeks 4 through 8 were 4011±361 eggs per gram of feces (epg) in wool lambs, and 1135±196 epg in hair lambs. PCV declined through week 7 in wool lambs but stabilized and then increased after week 4 in hair lambs. Mean PCV in weeks 4 through 8 were 22.4±0.3% in wool lambs and 24.3±0.5% in hair lambs. These results suggest that Caribbean hair breeds may be able to contribute significantly to development of parasite-resistant sheep populations.     

Lambs Fed Fresh Winter Forage Rape (Brassica napus L.) Emit Less Methane than Those Fed Perennial Ryegrass (Lolium perenne L.), and Possible Mechanisms behind the Difference

The objectives of this study were to examine long-term effects of feeding forage rape (Brassica napus L.) on methane yields (g methane per kg of feed dry matter intake), and to propose mechanisms that may be responsible for lower emissions from lambs fed forage rape compared to perennial ryegrass (Lolium perenne L.). The lambs were fed fresh winter forage rape or ryegrass as their sole diet for 15 weeks. Methane yields were measured using open circuit respiration chambers, and were 22-30% smaller from forage rape than from ryegrass (averages of 13.6 g versus 19.5 g after 7 weeks, and 17.8 g versus 22.9 g after 15 weeks). The difference therefore persisted consistently for at least 3 months. The smaller methane yields from forage rape were not related to nitrate or sulfate in the feed, which might act as alternative electron acceptors, or to the levels of the potential inhibitors glucosinolates and S-methyl L-cysteine sulfoxide. Ruminal microbial communities in forage rape-fed lambs were different from those in ryegrass-fed lambs, with greater proportions of potentially propionate-forming bacteria, and were consistent with less hydrogen and hence less methane being produced during fermentation. The molar proportions of ruminal acetate were smaller and those of propionate were greater in forage rape-fed lambs, consistent with the larger propionate-forming populations and less hydrogen production. Forage rape contained more readily fermentable carbohydrates and less structural carbohydrates than ryegrass, and was more rapidly degraded in the rumen, which might favour this fermentation profile. The ruminal pH was lower in forage rape-fed lambs, which might inhibit methanogenic activity, shifting the rumen fermentation to more propionate and less hydrogen and methane. The significance of these two mechanisms remains to be investigated. The results suggest that forage rape is a potential methane mitigation tool in pastoral-based sheep production systems.     

Effect of altering ruminal pH by dietary buffer supplementation on methane emissions from sheep fed forage rape

Low methane (CH₄) emissions from sheep fed forage rape (Brassica napus) might be related to low ruminal pH value. In this study, sodium carbonate (Na₂CO₃: SC) was supplemented to the diet to alter ruminal pH for evaluation of its role in CH₄ emissions from sheep fed forage rape. Fourteen intact and eight fistulated Romney sheep were adapted to forage rape over 32 days and then randomly allocated to one of two groups: diets supplemented with SC or not (control). Methane emissions were measured from intact sheep in seven experimental periods. In parallel, ruminal pH and fermentation characteristics were assessed using the fistulated sheep. In the first (P01) and the second (P02) periods, none of the sheep received SC to examine the baseline CH₄ emissions. The P01 period was used as a covariate for analysis of gas emission measurements in subsequent measurement periods. Sodium carbonate was offered at 5% of the forage DM in P03 and P04, increased to 8% in P05 and P06 to assess the effect of pH increase on CH₄ emissions and stopped in P07 to assess if the CH₄ emissions reverted to values similar to those measured before the supplementation started. Methane yield (g/kg forage DM intake) was similar for the sheep in both groups during P02 and P03, but sheep supplemented with SC in the diet emitted 36%, 49% and 30% more CH₄ per unit of forage DM intake than those in the control group during P04, P05 and P06, respectively. Emissions returned to similar levels when SC supplementation was ceased in P07. Ruminal pH was 0.412 to 0.565 units higher in SC supplemented sheep than for the control group during the SC treatment periods. Based on the lack of an immediate response in CH₄ emissions to the supplementation of SC in P03, the positive responses in P04 to P06 and the rapid disappearance of the response after supplementation with SC stopped in P07, we propose a new hypothesis that ruminal pH effects on CH₄ emissions are possibly through medium-term changes in microbial and methanogenic communities in the rumen, rather than a direct, short-term impact on methanogens per se. In conclusion, SC supplemented to the forage rape diet of sheep increased rumen pH, leading to an increase in CH₄ emissions. Low ruminal pH in sheep fed forage rape explains, at least partially, the reported low CH₄ emissions from sheep fed with this forage crop.     

The Role of Individual Traits and Environmental Factors for Diet Composition of Sheep

Large herbivore consumption of forage is known to affect vegetation composition and thereby ecosystem functions. It is thus important to understand how diet composition arises as a mixture of individual variation in preferences and environmental drivers of availability, but few studies have quantified both. Based on 10 years of data on diet composition by aid of microhistological analysis for sheep kept at high and low population density, we analysed how both individual traits (sex, age, body mass, litter size) linked to preference and environmental variation (density, climate proxies) linked to forage availability affected proportional intake of herbs (high quality/low availability) and Avenella flexuosa (lower quality/high availability). Environmental factors affecting current forage availability such as population density and seasonal and annual variation in diet had the most marked impact on diet composition. Previous environment of sheep (switch between high and low population density) had no impact on diet, suggesting a comparably minor role of learning for density dependent diet selection. For individual traits, only the difference between lambs and ewes affected proportion of A. flexuosa, while body mass better predicted proportion of herbs in diet. Neither sex, body mass, litter size, ewe age nor mass of ewe affected diet composition of lambs, and there was no effect of age, body mass or litter size on diet composition of ewes. Our study highlights that diet composition arises from a combination of preferences being predicted by lamb and ewes’ age and/or body mass differences, and the immediate environment in terms of population density and proxies for vegetation development.     

Nitrous oxide emission factors for urine and dung from sheep fed either fresh forage rape (Brassica napus L.) or fresh perennial ryegrass (Lolium perenne L.)

In New Zealand, agriculture is predominantly based on pastoral grazing systems and animal excreta deposited on soil during grazing have been identified as a major source of nitrous oxide (N₂O) emissions. Forage brassicas (Brassica spp.) have been increasingly used to improve lamb performance. Compared with conventional forage perennial ryegrass (Lolium perenne L.), a common forage in New Zealand, forage brassicas have faster growth rates, higher dry matter production and higher nutritive value. The aim of this study was to determine the partitioning of dietary nitrogen (N) between urine and dung in the excreta from sheep fed forage brassica rape (B. napus subsp. oleifera L.) or ryegrass, and then to measure N₂O emissions when the excreta from the two different feed sources were applied to a pasture soil. A sheep metabolism study was conducted to determine urine and dung-N outputs from sheep fed forage rape or ryegrass, and N partitioning between urine and dung. Urine and dung were collected and then used in a field plot experiment for measuring N₂O emissions. The experimental site contained a perennial ryegrass/white clover pasture on a poorly drained silt-loam soil. The treatments included urine from sheep fed forage rape or ryegrass, dung from sheep fed forage rape or ryegrass, and a control without dung or urine applied. N₂O emission measurements were carried out using a static chamber technique. For each excreta type, the total N₂O emissions and emission factor (EF3; N₂O–N emitted during the 3- or 8-month measurement period as a per cent of animal urine or dung-N applied, respectively) were calculated. Our results indicate that, in terms of per unit of N intake, a similar amount of N was excreted in urine from sheep fed either forage rape or ryegrass, but less dung N was excreted from sheep fed forage rape than ryegrass. The EF3 for urine from sheep fed forage rape was lower compared with urine from sheep fed ryegrass. This may have been because of plant secondary metabolites, such as glucosinolates in forage rape and their degradation products, are transferred to urine and affect soil N transformation processes. However, the difference in the EF3 for dung from sheep fed ryegrass and forage rape was not significant.     

Optimum dietary crude protein level for finishing Awassi lambs

Awassi is a multi-purpose sheep breed. Awassi lambs being finished are usually offered an 18% crude protein (CP) diet. The growth rate of Awassi lambs is lower than other meat breeds. Therefore, this high content of dietary CP is questionable. The objective of this study was to estimate the optimum CP level for finishing Awassi lambs. Fifty male Awassi lambs (23.0±1.2 kg) were fed five high concentrate isocaloric diets (10 lambs per diet) that contained 10, 12, 14, 16, and 18% CP in a totally mixed diets for 9 weeks using a completely randomized design. Lambs were fed twice daily, and feed offered and feed refusals recorded for each feeding. Individual lamb intakes were calculated using daily feed offered and feed refused averaged over the interval of the experiment. Digestibility estimates were measured by total fecal collection. Lambs fed diets that contained 10, 12, and 14% CP gained less weight than those fed the 16 and 18% CP diets (P<0.05). Dry matter and CP intakes increased (P<0.05) with increasing levels of dietary CP. No difference (P>0.10) was observed in feed-to-gain ratio between diets except for the diet that contained 10% CP (P<0.05) which had a lower ratio. Organic matter and CP digestibility were lowest in lambs fed the 10% CP diet. Results suggest that the optimum CP concentration is 16% and that any increase above this level will not result in any improvement in production.     

Supplementing high-quality fresh forage to growing lambs fed a total mixed ration diet led to higher intake without altering nutrient utilization

The effect of supplementing high-quality fresh forage, mainly based on alfalfa, to growing lambs fed with decreasing levels of total mixed ration (TMR) was studied on intake, digestion and ruminal environment. In total, 24 catheterized lambs (25.2±3.67 kg) housed in individual metabolism cages were assigned to one of four treatment diets: ‘TMR100’: TMR offered ad libitum; ‘TMR75’ and ‘TMR50’: TMR at a level of 0.75 and 0.50 of potential intake, respectively, complemented with fresh forage without restriction; ‘TMR0’: only fresh forage ad libitum. The feeding behavior, nutrient intake and digestibility, kinetics of passage and rumen environment were evaluated. As the level of TMR in the diet decreased, lambs increased the forage intake and spent more time eating and ruminating, less time resting and demonstrated a higher rate of intake. Those changes resulted in a higher nutrient intake of dry matter, organic matter, nitrogen, NDF and ADF, but a slightly lower organic matter digestibility, while no differences were detected in the output rate of particles. As a consequence, with the decrease of TMR and increase of forage intake, the ingested energy increased. Higher ruminal pH and NH₃–N concentrations were observed for lower levels of TMR in the diet. The total volatile fatty acids, acetate and propionate concentrations presented a quadratic response. Total volatile fatty acids and acetate concentrations were higher and propionate concentration was lower in lambs consuming mixed diets (TMR50 and TMR75). We concluded that the inclusion of high-quality fresh forage in a combined diet with TMR in lambs had positive effects on nutrient intake without negative consequences on digestion and rumen environment.     

Comparison of muscle fatty acid composition and lipid stability in lambs stall-fed or pasture-fed alfalfa with or without sainfoin pellet supplementation

Currently, consumers are increasingly interested in obtaining high-quality and healthy lamb meat. Compared to grain-based diets, dietary forage legumes such as alfalfa and condensed tannin (CT)-rich sainfoin increase the levels of polyunsaturated fatty acids (PUFAs) that are beneficial for health in lamb meat thanks to their high content in PUFA and/or their impact on ruminal biohydrogenation. However, they can therefore adversely affect its oxidative stability. Thus, the impact of dietary forage legumes on lamb longissimus thoracis (LT) muscle FA composition and their stability to peroxidation was studied in 36 Romane lambs grazing alfalfa (AF; n = 12) or alfalfa plus daily supplementation with CT-rich sainfoin pellets (AS; n = 12; 15 g DM/kg BW, 42 g CT/kg DM) or stall-fed concentrate and grass hay indoors (SI; n = 12). Lambs were slaughtered at a mean age of 162 ± 8.0 days after an average experimental period of 101 ± 8.1 days. Forage legumes-grazing lambs outperformed SI lambs in LT nutritional quality, with more conjugated linoleic acids and n-3 PUFAs, especially 18:3n-3, eicosapentaenoic and docosahexaenoic acids (P < 0.001), and thus lower n-6 PUFA/n-3 PUFA and 18:2 n-6/18:3 n-3 ratios (P < 0.001). Peroxidizability index was higher (P < 0.001) in LT muscle of forage legumes-grazing lambs. Concurrently, two endogenous antioxidant enzyme activities, superoxide dismutase and glutathione peroxidase, were, respectively, similar and lower (P < 0.001) for forage legumes-grazing compared with SI lambs. A lower vitamin E level in SI lambs compared with forage legumes-grazing lambs (1.0 v. 3.8 mg/g, P < 0.001) could explain that malondialdehyde content, a marker of lipid oxidation intensity, was 0.63 µg/g in SI after 8 days in aerobic packaging conditions, whereas it remaining steady at 0.16 µg/g in forage legumes-grazing lambs. Dietary forage alfalfa thus improved FA composition of lamb LT muscle and their stability to oxidation when compared to SI lambs. However, supplementation of alfalfa-grazing lambs with CT-rich sainfoin pellets did not affect the nutritional quality of LT muscle FAs.     

Susceptibility of Young Sheep to Oral Infection with Bovine Spongiform Encephalopathy Decreases Significantly after Weaning

Bovine spongiform encephalopathy (BSE) is a transmissible spongiform encephalopathy (TSE) (or prion disease) that is readily transmissible to sheep by experimental infection and has the shortest incubation period in animals with the ARQ/ARQ PRNP genotype (at codons 136, 154, and 171). Because it is possible that sheep in the United Kingdom could have been infected with BSE by being fed contaminated meat and bone meal supplements at the same time as cattle, there is considerable interest in the responses of sheep to BSE inoculation. Epidemiological evidence suggests that very young individuals are more susceptible to TSE infection; however, this has never been properly tested in sheep. In the present study, low doses of BSE were fed to lambs of a range of ages (∼24 h, 2 to 3 weeks, 3 months, and 6 months) and adult sheep. The incidence of clinical BSE disease after inoculation was high in unweaned lambs (∼24 h and 2 to 3 weeks old) but much lower in older weaned animals The incubation period was also found to be influenced by the genotype at codon 141 of the PRNP gene, as lambs that were LF heterozygotes had a longer mean incubation period than those that were homozygotes of either type. The results suggest that sheep in the United Kingdom would have been at high risk of BSE infection only if neonatal animals had inadvertently ingested contaminated supplementary foodstuffs.     

The use of Balanites kernel cake in a diet for fattening sheep

Balanites kernel cake (BKC) is produced from the fruit of Balanites aegyptiaca, a tree which is widely and densely distributed along the tropical belt of the African continent. The cake is characterized by high protein (36.8%) and low crude fibre (5.9%) contents. In this work the value of BKC as a source of crude protein in ruminant diets was compared with cotton seed cake (CSC), a conventional source of protein. Two groups of 10 and 11 lambs having an average liveweight of 20.5 ± 0.9 kg were used. For a 3-week preliminary period animals of both groups were fed ad libitum on a diet containing 30% CSC as a major source of protein. During the following 13 weeks the group of 10 lambs continued on the same diet, whereas the other group was fed ad libitum on a Balanites cake diet (20% BKC). All lambs were slaughtered at about 40 kg liveweight. No significant differences were detected between the two groups of lambs in feedlot performance or carcass analysis.It is concluded that BKC is non toxic to sheep and its addition at the rate of 20%, together with 10% straw, can replace 30% CSC in the diet. The Balanites cake diet was also found to be substantially cheaper.     

Behavioural and developmental responses of range caterpillar larvae, Hemileuca oliviae, to condensed tannin

Condensed tannin and astringency levels of several grasses known to be preferred, or not preferred, by range caterpillar larvae, Hemileuca oliviae Cockrell, were determined. Comparable levels of a commercially available condensed tannin, quebracho, were applied to filter paper discs in choice-tests. Quebracho inhibited feeding in preference tests. Quebracho also was incorporated into agar-based diets; larval development and survival were inhibited. However, in these latter tests, larvae were not provided with tannin-free diet and responded to the presence of quebracho by increasing their rate of consumption. Condensed tannin (quebracho) therefore acted as a feeding deterrent to range caterpillar larvae in situations where low-tannin hosts were available, but as an antimetabolite in no-choice environments.     

Relative bioavailability of copper in a copper-lysine complex or copper sulfate for ruminants as affected by feeding regimen

An experiment was conducted with 40 crossbred wether lambs to compare the relative bioavailability of Cu from a Cu-lysine complex with that from Cu sulfate under two feeding regimens. Liver biopsy samples were collected by a laparotomy technique and lambs were given 12 days to recover and adapt to individual pens. Lambs were fed either a control basal diet (9.8 mg kg⁻¹ Cu dry matter (DM) basis) or basal supplemented with 180 mg kg⁻¹ Cu as either reagent grade CuSO₄ · 5H₂O or a feed-grade Cu-lysine complex. There were 12 lambs fed control diet and 14 lambs fed each Cu-supplemented diet. Half of the lambs given each diet were fed 1.0 kg of their respective diet once daily at 08:00 h. The remaining half were fed 1.0 kg in equal portions (166 g) six times during a 24 h period at 04:00 h, 08:00 h, 12:00 h, 16:00 h, 20:00 h and 24:00 h. Lambs were fed experimental diets for 10 days, then killed and livers removed for Cu analysis. Lambs fed once daily accumulated more (P < 0.05) Cu in liver than those fed every 4 h. There was no Cu source × feeding regimen interaction (P > 0.10). Based on multiple linear regression slope ratio of log₁₀ transformed final liver Cu concentration on total intake of Cu (mg day⁻¹) with the initial liver biopsy Cu concentration as a covariate, bioavailability of Cu from Cu-lysine relative to Cu sulfate was 93.4% (P > 0.05) for combined feeding regimens.     

Dietary protein or energy restriction influences age and weight at puberty in ewe lambs

The working hypothesis was that restricted protein content in diets of prepubertal ewe lambs would delay time of onset of puberty to a greater extent than restricted energy content. Restriction of dietary protein (metabolizable protein, MP) or energy (ME) intake on age and weight at puberty was studied in ewe lambs in two experiments. In Experiment 1, 85 crossbred ewe lambs were assigned by weight to one of five dietary treatment groups: control (CTL; 18 mg ME day⁻¹ and 173 g MP day⁻¹); energy restricted (ER; 10.2 mg ME day⁻¹ and 96 g MP day⁻¹); protein restricted (PR; 11 mg ME day⁻¹ and 66 g MP day⁻¹); ER for 9 weeks then switched to CTL (ER/CTL); PR for 9 weeks then switched to CTL (PR/CTL). Ewe lambs were weighed biweekly, bled weekly, and serum was assayed for progesterone to determine time of puberty. Average age at puberty differed among lambs fed the various diets (P < 0.01). Ewe lambs in the CTL group were younger and weighed more at puberty. Lambs fed diets restricted in protein or energy content were similar in age at puberty. Dietary switching, ER/CTL and PR/CTL, affected body weight and gain (P < 0.05), but did not result in changed age at puberty compared with lambs fed ER or PR diets. In Experiment 2, two breed types (maternal type and meat type) were used to compare responses of dietary protein restriction to dietary energy restriction. In Experiment 2, lambs (n = 60) were assigned to either the CTL, PR or ER diets. Lambs in the CTL group gained more weight than lambs fed the PR diet and lambs fed the PR diet gained more weight than lambs fed the ER diet (P < 0.05). Diet affected age (P < 0.001), but not weight at puberty (P = 0.13). Dietary energy and protein restriction influenced age at puberty in ewe lambs with energy restriction having a greater influence on delaying onset of puberty than protein restriction. Realimentation did not overcome effects of previous feed restriction by inducing an earlier age at puberty than ewe lambs fed the ER or PR diet throughout the experimental period. Body weight gain prepuberty is more important than type of dietary restriction (protein or energy) with regard to impacting age at puberty. We, therefore, reject the working hypothesis that dietary protein restriction prepuberty would have a greater impact in delaying onset of puberty than dietary energy restriction.     

Effects of defaunation on digestion of fresh Digitaria decumbens grass and growth of lambs

The effects of defaunation on growth and digestion were measured in sheep fed fresh Digitaria decumbens grass cut at four stages of regrowth 14, 28, 42, and 56 days, and with different protein to energy (P/E) ratios. Two completely randomized designs trials (growth and digestion) were conducted using faunated animals, defaunated rams and protozoa free lambs. The digestion trial: eight faunated and eight defaunated rams fitted with ruminal and duodenal cannulas were fed 4 diets (diets D1 to D4) to measure digestion parameters. The dietary P/E ratios were 120 (D1), 130 (D2), 130 (D3), and 140 (D4) g PDIN/UFL (Protein Digested in the small Intestine supplied by microbial protein from rumen-degraded protein/Feed Unit for Lactation) and the grass stages of regrowth were 56, 42, 28, and 14 days, respectively. Increasing the dietary P/E ratios increased dry matter intake (DMI) and the total tract digestibility of organic matter (OM), NDF and CP. Defaunation decreased DMI, except for rams fed D4 diet. Defaunation also decreased total tract digestibility of OM except for rams fed D1 diet and that of NDF except for rams fed D1 and D4 diets. Increasing the dietary P/E ratios also increased nitrogen intake and ammonia (NH3) concentration in the rumen, whereas defaunation decreased them. The dietary P/E ratio increased non-NH3 nitrogen and microbial nitrogen duodenal flows and microbial efficiency. Defaunation did not affect duodenal flows of neither non-NH3 nitrogen and microbial nitrogen or microbial efficiency. The growth trial: 20 faunated and 20 protozoa free lambs were fed four diets (diets D5 to D8) to measure their average daily gain (ADG). The dietary P/E ratios were 60 (D5), 70 (D6), 80 (D7) and 100 (D8) g PDIN/UFL and the stages of regrowth were 56, 42, 28, and 14 days, respectively. DMI of lambs increased with P/E ratio. Protozoa free lambs had greater DMI than faunated ones when fed D7 diet (80.8 v. 74.9 g/kg LW0.75, respectively). The ADG of the lambs increased with P/E ratio. Fed on the same D5 diet, protozoa free lambs had greater ADG than faunated lambs (29.8 v. 11.6 g/day, respectively). In conclusion, animal response to defaunation was modulated by the P/E ratio of the D. decumbens grass diets. Defaunation increased ADG of lambs fed forage with the lowest P/E ratio, while digestion and nitrogen duodenal flows of rams fed the lowest P/E ratio were not affected.     

Effects of restricted feeding of prepubertal ewe lambs on growth performance and mammary gland development

The aim of this study was to determine the effects of restricted feeding before puberty on growth performance and mammary gland development in replacement ewe lambs. At weaning, 72 Dorset ewe lambs were assigned to one of the three diets: an ad libitum control diet with medium-quality forage (MQF; diet A-MQF); a restricted diet with the same forage as A, but less feed concentrate (diet R-MQF); or a high-quality forage (HQF) diet (diet F-HQF). The quantity of concentrate offered to the group R-MQF and F-HQF ewe lambs was adjusted to obtain 70% of the control ewe lambs' growth rate. The diets were offered for 75 days after weaning to cover the allometric phase of mammary gland development. During this period, average daily gain (ADG) was 223 and 229 g/day for groups R-MQF and F-HQF, respectively, compared to 305 g/day for group A-MQF (P < 0.0001). At the end of this period, 28 ewe lambs were slaughtered and their mammary gland was collected. Parenchymal fresh tissue weight tended to be higher for groups R-MQF and F-HQF compared to group A-MQF (P = 0.09). Stroma weight was greater (P < 0.05) for the group A-MQF ewe lambs than for those in the other treatments. Total DNA and total protein in parenchymal tissue tended to be greater for groups R-MQF and F-HQF (P = 0.09 and P = 0.07, respectively). Dry fat-free tissue was greater for groups R-MQF and F-HQF (P < 0.05). The remaining ewe lambs were fed the same haylage and barley diet until their first lambing. During this period, compensatory growth was observed. ADG was greater (P < 0.01) for groups R-MQF and F-HQF than for group A-MQF, and feed conversion was improved (P < 0.01) for groups R-MQF and F-HQF compared with the control, whereas the dry matter intake was similar for all groups. Live body weight, loin eye depth and backfat depth at breeding and around lambing were similar for all groups. The results of this study suggest that restricted feeding before puberty improves mammary gland development without compromising growth performance in ewe lambs.     

The effect of replacing rice straw with dry wormwood (Artemisia sp.) on intake,digestibility, nitrogen balance and ruminal fermentation characteristics in sheep

This study examined the effect of substituting dry wormwood (Artemisia sp.) for rice straw in sheep diets on intake and apparent digestibility in vivo, nitrogen (N) balance and ruminal fermentation characteristics. Four Corriedale×Polwarth sheep (51.7 ± 1.3 kg) were individually housed in metabolism cages and fed diets (ad. libitum) with a 70:30 forage to concentrate ratio (DM basis), in which the basal rice straw was substituted with 0 (Control), 30 (LW), 50 (MW) or 100 (HW) g/kg of dry wormwood. The experimental design was a 4×4 latin square design in which 10 days of dietary adaptation was followed by 6 days of total feces and urine collection in each period. Rumen fluid was collected from a stomach tube at −0.5, 0.5, 1, 2, 4, and 8 h after the morning feed on day 6 of each collection period and analyzed for volatile fatty acids (VFA). The intakes of dry matter (DM), organic matter intake (OM), crude protein (CP), ether extract (EE), neutral detergent fibre (NDF) and acid detergent fibre (ADF) were higher (P<0.05) in sheep fed diets containing wormwood, than those fed the Control diet. Compared to the Control diet, CP digestibility was higher (P<0.05) in sheep fed MW and N intake, retained N, EE digestibility, urinary purine derivatives and microbial N yield were higher (P<0.05) in sheep fed diets, containing wormwood. Rumen pH was unaffected by treatment. Rumen NH₃-N and VFA concentrations were similar across treatments except that most values for diets containing wormwood were higher (P<0.05) than those for the control diet within the first 2 h of feeding. The non-glucogenic acid ratio was also similar across Control, LW and MW treatments, but it was generally lower in MW versus the Control treatment. In conclusion, substituting wormwood for rice straw in the sheep diets increased feed intake, rumen fermentation, in vivo digestibility, N retention and microbial N yield, particularly at the medium and high levels of wormwood inclusion.     

Effect of different dietary tannin extracts on lamb growth performances and meat oxidative stability: comparison between mimosa,chestnut and tara

Little information is available on the effects of different sources of tannins on ruminant product quality. Nowadays several tannin-rich extracts, produced from different plants, are available and contain tannins belonging to different chemical groups, but most of these have not been used so far as feed supplements. The present study aimed at comparing the effects of feeding three tannin extracts (one containing condensed tannins and two containing hydrolysable tannins) to lambs on growth performances and meat oxidative stability. Comisana male lambs were divided into four groups (n=9 each) and were fed for 75 days: a concentrate-based diet (CON), or CON supplemented with 4% tannin extracts from either mimosa (MI; Acacia mearnsii, De Wild; condensed tannins), chestnut (CH; Castanea sativa, Mill; hydrolysable ellagitannins) or tara (TA; Cesalpinia spinosa, (Molina) Kuntze; hydrolysable gallotannins). Only CH reduced growth rate, final weight, carcass weight and feed intake (P<0.05). Tannins did not affect the concentration of the main fatty acid classes and the peroxidability of the intramuscular fat (P>0.05). The TA diet increased (P<0.001) the concentration of γ-tocopherol in muscle and tended to increase that of α-tocopherol (P=0.058). Oxidative stability of raw and cooked meat, or of meat homogenates incubated with pro-oxidants, was not affected by the extracts. These results, compared with those reported in the literature, highlight that some effects of tannins cannot be easily generalized, but may strictly depend on their specific characteristics and on conditions inherent to the basal diet and the metabolic status of the animals.