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1.
Development of electrical coupling and action potential synchrony between paired aggregates of embryonic heart cells 总被引:2,自引:0,他引:2
Summary Pairs of spheroidal aggregates of embryonic chick heart cells, held in suction pipettes were brought into contact and allowed to synchronize their spontaneous action potentials. Contractions were suppressed with cytochalasin B. Both intracellular and extracellular electrodes were used to analyze the development of synchrony. Electric coupling occurred in three phases. During phase I electrical interactions were absent despite close physical contact. Phase II was characterized by partial synchrony. Action potentials in the faster aggregate (F) induced small depolarizations in the other member of the pair (S). These depolarizations sometimes triggered action potentials inS depending on when during the diastolic depolarization inS they occurred. In these cases both the latency between the action potentials (L) and the fluctuations in latency (V
L) were large. At the end of phase II the aggregates often passed through a brief period when fluctuation in interbeat interval in both increased noticeably. In phase III, beginning about 8 min after initial contact, action potentials were completely entrained at a certainL. During the subsequent 20–40 minL fell along an approximately exponential time course from about 130 to <1 msec, whileV
L declined in parallel. When well-coupled aggregates were pulled apart and immediately pressed back together, they re-established synchronization according to the usual three-phase time course. Synchronized aggregates could be partially decoupled by separating them just far enough to reduce the area of mutual contact. Pairs joined only by cellular strands maintained entrained action potentials with long latencies for many minutes. These results indicate that electronic junctions form between the paired heart cell aggregates causing the gradual development of action potential synchrony. 相似文献
2.
Circadian (ca. 24 hr) oscillations in expression of mammalian "clock genes" are found not only in the suprachiasmatic nucleus (SCN), the central circadian pacemaker, but also in peripheral tissues. Under constant conditions in vitro, however, rhythms of peripheral tissue explants or immortalized cells damp partially or completely. It is unknown whether this reflects an inability of peripheral cells to sustain rhythms, as SCN neurons can, or a loss of synchrony among cells. Using bioluminescence imaging of Rat-1 fibroblasts transfected with a Bmal1::luc plasmid and primary fibroblasts dissociated from mPer2(Luciferase-SV40) knockin mice, we monitored single-cell circadian rhythms of clock gene expression for 1-2 weeks. We found that single fibroblasts can oscillate robustly and independently with undiminished amplitude and diverse circadian periods. Cells were partially synchronized by medium changes at the start of an experiment, but due to different intrinsic periods, their phases became randomly distributed after several days. Closely spaced cells in the same culture did not have similar phases, implying a lack of functional coupling among cells. Thus, like SCN neurons, single fibroblasts can function as independent circadian oscillators; however, lack of oscillator coupling in dissociated cell cultures leads to a loss of synchrony among individual cells and damping of the ensemble rhythm at the population level. 相似文献
3.
During different behavioral states different population activities are present in the hippocampal formation. These activities
are not independent: sharp waves often occur together with high-frequency ripples, and gamma-frequency activity is usually
superimposed on theta oscillations. There is both experimental and theoretical evidence supporting the notion that gamma oscillation
is generated intrahippocampally, but there is no generally accepted view about the origin of theta waves. Precise timing of
population bursts of pyramidal cells may be due to a synchronized external drive. Membrane potential oscillations recorded
in the septum are unlikely to fulfill this purpose because they are not coherent enough. We investigated the prospects of
an intrahippocampal mechanism supplying pyramidal cells with theta frequency periodic inhibition, by studying a model of a
network of hippocampal inhibitory interneurons. As shown previously, interneurons are capable of generating synchronized gamma-frequency
action potential oscillations. Exciting the neurons by periodic current injection, the system could either be entrained in
an oscillation with the frequency of the inducing current or exhibit in-phase periodic changes at the frequency of single
cell (and network) activity. Simulations that used spatially inhomogeneous stimulus currents showed anti-phase frequency changes
across cells, which resulted in a periodic decrease in the synchrony of the network. As this periodic change in synchrony
occurred in the theta frequency range, our network should be able to exhibit the theta-frequency weakening of inhibition of
pyramidal cells, thus offering a possible mechanism for intrahippocampal theta generation.
Received: 23 February 2000 / Accepted in revised form: 30 June 2000 相似文献
4.
How do humans and other animals accomplish coordinated movements? How are novel combinations of limb joints rapidly assembled
into new behavioral units that move together in in-phase or anti-phase movement patterns during complex movement tasks? A
neural central pattern generator (CPG) model simulates data from human bimanual coordination tasks. As in the data, anti-phase
oscillations at low frequencies switch to in-phase oscillations at high frequencies, in-phase oscillations occur at both low
and high frequencies, phase fluctuations occur at the anti-phase in-phase transition, a “seagull effect” of larger errors
occurs at intermediate phases, and oscillations slip toward in-phase and anti-phase when driven at intermediate phases. These
oscillations and bifurcations are emergent properties of the CPG model in response to volitional inputs. The CPG model is
a version of the Ellias-Grossberg oscillator. Its neurons obey Hodgkin-Huxley type equations whose excitatory signals operate
on a faster time scale than their inhibitory signals in a recurrent on-center off-surround anatomy. When an equal command
or GO signal activates both model channels, the model CPG can generate both in-phase and anti-phase oscillations at different
GO amplitudes. Phase transitions from either in-phase to anti-phase oscillations, or from anti-phase to in-phase oscillations,
can occur in different parameter ranges, as the GO signal increases.
Received: 22 August 1994 / Accepted in revised form: 13 May 1997 相似文献
5.
Pittendrigh first found that the circadian rhythm of locomotor activity in nocturnal rodents split into two components. Hoffman then reported that the splitting phenomenon was even more reproducible in the small diurnal primate Tupaia. These “splitting” experiments and many other experiments suggest that two coupled oscillators may constitute the circadian pacemaker system. Pittendrigh proposed a phenomenological two-oscillator model. Daan and Berde developed a quantitative model assuming that the interaction between the two constituent oscillators is by instantaneous resets. Their model system can simulate several qualitative features in the experimental data. As the assumption of instantaneous resets seems to be unnatural, we study two limit cycle oscillators, which are coupled continuously to each other, as a model of the circadian pacemaker. We assume the following points, (i) One oscillator in a resting state does not affect another oscillator, (ii) Two oscillators are identical, (iii) The coupling is symmetrical. By the theory of Hopf bifurcation it is found that the general two-oscillator system has two stable periodic solutions. One is the in-phase solution where the two constituent oscillators oscillate in phase synchrony. Another is the anti-phase solution where the two oscillators oscillate 180 ° out of phase. The former corresponds to a single pattern of locomotor activity and the latter corresponds to a splitting pattern. Furthermore, we study specific two-neural oscillators, which are linearly coupled to each other. By the method of secondary bifurcation we find that the model shows simultaneous stability of the two alternative phase relationships and the hysteresis phenomena found in Tupaia. A natural period of the uncoupled constituent oscillator is longer than that of the in-phase solution but it is shorter than that of the anti-phase solution. This is in agreement with the data of Tupaia. 相似文献
6.
In mammals, the circadian pacemaker, which controls daily rhythms, is located in the suprachiasmatic nucleus (SCN). Circadian oscillations are generated in individual SCN neurons by a molecular regulatory network. Cells oscillate with periods ranging from 20 to 28 h, but at the tissue level, SCN neurons display significant synchrony, suggesting a robust intercellular coupling in which neurotransmitters are assumed to play a crucial role. We present a dynamical model for the coupling of a population of circadian oscillators in the SCN. The cellular oscillator, a three-variable model, describes the core negative feedback loop of the circadian clock. The coupling mechanism is incorporated through the global level of neurotransmitter concentration. Global coupling is efficient to synchronize a population of 10,000 cells. Synchronized cells can be entrained by a 24-h light-dark cycle. Simulations of the interaction between two populations representing two regions of the SCN show that the driven population can be phase-leading. Experimentally testable predictions are: 1), phases of individual cells are governed by their intrinsic periods; and 2), efficient synchronization is achieved when the average neurotransmitter concentration would dampen individual oscillators. However, due to the global neurotransmitter oscillation, cells are effectively synchronized. 相似文献
7.
The medial septum-diagonal band (MSDB) complex is considered as a pacemaker for the hippocampal theta rhythm. Identification
of the different cell types, their electro-physiological properties and their possible function in the generation of a synchronized
activity in the MSDB is a hot topic. A recent electro-physiological study showed the presence of two antiphasically firing
populations of parvalbumin containing GABAergic neurons in the MSDB. Other papers described a network of cluster-firing glutamatergic
neurons, which is able to generate synchronized activity in the MSDB. We propose two different computer models for the generation
of synchronized population theta oscillation in the MSDB and compare their properties. In the first model GABAergic neurons
are intrinsically theta periodic cluster-firing cells; while in the second model GABAergic cells are fast-firing cells and
receive periodic input from local glutamatergic neurons simulated as cluster-firing cells. Using computer simulations we show
that the GABAergic neurons in both models are capable of generating antiphasic theta periodic population oscillation relying
on local, septal mechanisms. In the first model antiphasic theta synchrony could emerge if GABAergic neurons form two populations
preferentially innervate each other. In the second model in-phase synchronization of glutamatergic neurons does not require
specific network structure, and the network of these cells are able to act as a theta pacemaker for the local fast-firing
GABAergic circuit. Our simulations also suggest that neurons being non-cluster-firing in vitro might exhibit clustering properties when connected into a network in vivo.
Action Editor: David Golomb 相似文献
8.
Arthur Sherman 《Bulletin of mathematical biology》1994,56(5):811-835
I seek to explain phenomena observed in simulations of populations of gap junction-coupled bursting cells by studying the
dynamics of identical pairs. I use a simplified model for pancreatic β-cells and decompose the system into fast (spike-generating)
and slow subsystems to show how bifurcations of the fast subsystem affect bursting behavior. When coupling is weak, the spikes
are not in phase but rather are anti-phase, asymmetric or quasi-periodic. These solutions all support bursting with smaller
amplitude spikes than the in-phase case, leading to increased burst period. A key geometrical feature underlying this is that
the in-phase periodic solution branch terminates in a homoclinic orbit. The same mechanism also provides a model for bursting
as an emergent property of populations; cells which are not intrinsic bursters can burst when coupled. This phenomenon is
enhanced when symmetry is broken by making the cells differ in a parameter. 相似文献
9.
We synchronized Drosophila cell lines (Schneider's line 2 and Kc) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S = 14 to 16 hr; G2 = 6 to 8 hr; M = 0.4 hr) were similar to those of Kc cells. 相似文献
10.
Synchronization between neuronal populations plays an important role in information transmission between brain areas. In particular, collective oscillations emerging from the synchronized activity of thousands of neurons can increase the functional connectivity between neural assemblies by coherently coordinating their phases. This synchrony of neuronal activity can take place within a cortical patch or between different cortical regions. While short-range interactions between neurons involve just a few milliseconds, communication through long-range projections between different regions could take up to tens of milliseconds. How these heterogeneous transmission delays affect communication between neuronal populations is not well known. To address this question, we have studied the dynamics of two bidirectionally delayed-coupled neuronal populations using conductance-based spiking models, examining how different synaptic delays give rise to in-phase/anti-phase transitions at particular frequencies within the gamma range, and how this behavior is related to the phase coherence between the two populations at different frequencies. We have used spectral analysis and information theory to quantify the information exchanged between the two networks. For different transmission delays between the two coupled populations, we analyze how the local field potential and multi-unit activity calculated from one population convey information in response to a set of external inputs applied to the other population. The results confirm that zero-lag synchronization maximizes information transmission, although out-of-phase synchronization allows for efficient communication provided the coupling delay, the phase lag between the populations, and the frequency of the oscillations are properly matched. 相似文献
11.
Cell cycle dependence of cytotoxicity and clastogenicity induced by treatment of synchronized human diploid fibroblasts with sodium fluoride 总被引:11,自引:0,他引:11
To study the cell cycle dependence of cytotoxicity and clastogenicity of sodium fluoride (NaF), synchronized human diploid fibroblasts were treated with NaF during different phases of the cell cycle and analyzed. Exponentially growing cells were synchronized by the following two procedures. (1) The cells were synchronized at G0/G1 phase by a period of growth in medium containing 1% serum (low serum medium). (2) The cells were synchronized at the G1/S boundary by growth in low serum medium, followed by hydroxyurea treatment (Tsutsui et al., 1984a). Synchronized cells were treated with NaF for 3 h during the G1 phase or G2 phase, and for each of three 3-h periods during the S phase which lasted 9 h. Cytotoxicity, as determined by a decrease in colony-forming ability, was dependent upon the phase of the cell cycle during which NaF treatment was administered. The highest lethality was induced in when the cultures were treated with NaF during the second or third 3 h of S phase (middle or late S phase, respectively), or G2 phase. Little lethality was observed in cultures in G1 phase. Inducibility of chromosome aberrations of the cells following treatment with NaF was also dependent upon the phase of the cell cycle. A significant increase in the incidence of chromosome aberrations was observed only in cultures treated with NaF during early and / or middle S phases of cell cycle. These results suggest that cytotoxicity and clastogenicity of NaF to cultured human diploid fibroblasts are cell cycle dependent, and that the cells in early and middle S phases are more sensitive to the effects. 相似文献
12.
D J Grdina M L Meistrich R E Meyn T S Johnson R A White 《Cell and tissue kinetics》1984,17(3):223-236
Selected cell synchrony techniques, as applied to asynchronous populations of Chinese hamster ovary (CHO) cells, have been compared. Aliquots from the same culture of exponentially growing cells were synchronized using mitotic selection, mitotic selection and hydroxyurea block, centrifugal elutriation, or an EPICS V cell sorter. Sorting of cells was achieved after staining cells with Hoechst 33258. After synchronization by the various methods the relative distribution of cells in G1, S, or G2 + M phases of the cell cycle was determined by flow cytometry. Fractions of synchronized cells obtained from each method were replated and allowed to progress through a second cell cycle. Mitotic selection gave rise to relatively pure and unperturbed early G1 phase cells. While cell synchrony rapidly dispersed with time, cells progressed through the cell cycle in 12 hr. Sorting with the EPICS V on the modal G1 peak yielded a relatively pure but heterogeneous G1 population (i.e. early to late G1). Again, synchrony dispersed with time, but cell-cycle progression required 14 hr. With centrifugal elutriation, several different cell populations synchronized throughout the cell cycle could be rapidly obtained with a purity comparable to mitotic selection and cell sorting. It was concluded that, either alone or in combination with blocking agents such as hydroxyurea, elutriation and mitotic selection were both excellent methods for synchronizing CHO cells. Cell sorting exhibited limitations in sample size and time required for synchronizing CHO cells. Its major advantage would be its ability to isolate cell populations unique with respect to selected cellular parameters. 相似文献
13.
We numerically investigate the influence of intrinsic channel noise on the dynamical response of delay-coupling in neuronal systems. The stochastic dynamics of the spiking is modeled within a stochastic modification of the standard Hodgkin–Huxley model wherein the delay-coupling accounts for the finite propagation time of an action potential along the neuronal axon. We quantify this delay-coupling of the Pyragas-type in terms of the difference between corresponding presynaptic and postsynaptic membrane potentials. For an elementary neuronal network consisting of two coupled neurons we detect characteristic stochastic synchronization patterns which exhibit multiple phase-flip bifurcations: The phase-flip bifurcations occur in form of alternate transitions from an in-phase spiking activity towards an anti-phase spiking activity. Interestingly, these phase-flips remain robust for strong channel noise and in turn cause a striking stabilization of the spiking frequency. 相似文献
14.
Very little is known about the metabolism of phospholipids in the G2 and M phases of the cell cycle, but limited studies have led to the postulation that phospholipid synthesis ceases during this period. To investigate whether phospholipids are synthesized in the G2/M phase of the cell cycle, protocols were developed to produce synchronized MCF-7 cell populations with greater than 80% of the cells in G1/S or G2/M phases that moved in synchrony following removal of the blocking agent. Analysis of the activities of key phosphatidylcholine and phosphatidylethanolamine biosynthetic enzymes in subcellular fractions obtained from MCF-7 cells at different cell cycle phases revealed that there was robust activity of key enzymes in the fractions prepared from MCF-7 cells in G2/M phase. Radiolabeled choline and ethanolamine were rapidly incorporated into cells maintained at G2/M phase with nocodazole, and the rates of incorporation were similar to those obtained in cells allowed to progress into the G1 phase. Furthermore, radiolabeled glycerol was incorporated into phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and phosphatidic acid in MCF-7 cells maintained at G2/M phase with nocodazole. Similar results were obtained in CHO cells. These results demonstrate that glycerophospholipid synthesis is very active in the G2/M phase of these cells. Therefore, the postulated cessation of phospholipid synthesis in G2/M phases is not applicable to all cell types. 相似文献
15.
Using two-cell and 50-cell networks of square-wave bursters, we studied how excitatory coupling of individual neurons affects
the bursting output of the network. Our results show that the effects of synaptic excitation vs. electrical coupling are distinct.
Increasing excitatory synaptic coupling generally increases burst duration. Electrical coupling also increases burst duration
for low to moderate values, but at sufficiently strong values promotes a switch to highly synchronous bursts where further
increases in electrical or synaptic coupling have a minimal effect on burst duration. These effects are largely mediated by
spike synchrony, which is determined by the stability of the in-phase spiking solution during the burst. Even when both coupling
mechanisms are strong, one form (in-phase or anti-phase) of spike synchrony will determine the burst dynamics, resulting in
a sharp boundary in the space of the coupling parameters. This boundary exists in both two cell and network simulations. We
use these results to interpret the effects of gap-junction blockers on the neuronal circuitry that underlies respiration. 相似文献
16.
Ishii Y Yamagishi H 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2002,133(3):589-594
The heart of the ostracod crustacean Vargula hilgendorfii has a single intrinsic neuron that morphologically appears to innervate the myocardium. We, therefore, examined the heart activity electrophysiologically to determine whether the heartbeat is neurogenic. Each heartbeat is associated with a myocardial action potential composed of a spike potential followed by a plateau potential. The frequency of the action potential is not stable but changes successively over a wide range. The action potential is not preceded by a pacemaker potential and has an inflection in its rising phase. The myocardial cells couple electrically and fire almost simultaneously. The frequency of the action potential was unchanged by injection of depolarizing or hyperpolarizing current into the myocardium. However, slow oscillatory potentials appeared during the depolarization and its frequency was higher with increasing current intensity. Application of 1-microM tetrodotoxin (TTX) depolarized the myocardial membrane and completely prevented the action potential. During this depolarization, slow oscillatory potentials often appeared spontaneously. These results suggest that, although the myocardium has a property of conditional oscillator, the heartbeat is driven by the single cell cardiac ganglion that has both pacemaker and motor functions. 相似文献
17.
Schoppa NE 《Neuron》2006,49(2):271-283
Synchronized oscillatory activity at the gamma frequency (30-70 Hz) is thought to be important for information processing in many sensory systems. Here, I used patch-clamp recordings in neuron pairs in rat olfactory bulb slices to assess the mechanisms underlying such "gamma" activity in the olfactory system. Patterned electrical stimulation of afferents that mimicked a natural odor stimulus elicited rapidly synchronized spikes (lag < or = 5 ms) in mitral cells, along with oscillatory activity at the gamma (approximately 50 Hz) frequency. Analysis of coupling potentials, combined with dendritic sectioning, indicated that mitral cell synchrony was mainly driven by inhibitory postsynaptic potentials (IPSPs) imposed by GABAergic granule cells. Recordings in granule cell pairs indicated that granule cells were themselves synchronized by their excitatory inputs from mitral cells, providing a means to coordinate GABA release. These results demonstrate that rapid synchrony can emerge in a network through the precise back-and-forth interplay between neuronal populations. 相似文献
18.
Observations in cultured mouse astrocytes suggest anti-phase synchronization of cytosolic calcium concentrations in nearest neighbor cells that are coupled through gap junctions. A mathematical model is used to investigate physiologic conditions under which diffusion of the second messenger inositol (1, 4, 5)-trisphosphate (IP(3)) through gap junctions can facilitate synchronized anti-phase Ca(2+) oscillations. Our model predicts anti-phase oscillations in both cytosolic calcium and IP(3) concentrations if (a) the gap junction permeability is within a window of values and (b) IP(3) is regenerated in the astrocytes via, e.g. phospholipase C(delta). This result sheds new light on the current dispute on the mechanism of intercellular calcium signaling. It provides indirect evidence for a partially regenerative mechanism as the model excludes anti-phase synchrony in the absence of IP(3) regeneration. 相似文献
19.
The ability of spiking neurons to synchronize their activity in a network depends on the response behavior of these neurons as quantified by the phase response curve (PRC) and on coupling properties. The PRC characterizes the effects of transient inputs on spike timing and can be measured experimentally. Here we use the adaptive exponential integrate-and-fire (aEIF) neuron model to determine how subthreshold and spike-triggered slow adaptation currents shape the PRC. Based on that, we predict how synchrony and phase locked states of coupled neurons change in presence of synaptic delays and unequal coupling strengths. We find that increased subthreshold adaptation currents cause a transition of the PRC from only phase advances to phase advances and delays in response to excitatory perturbations. Increased spike-triggered adaptation currents on the other hand predominantly skew the PRC to the right. Both adaptation induced changes of the PRC are modulated by spike frequency, being more prominent at lower frequencies. Applying phase reduction theory, we show that subthreshold adaptation stabilizes synchrony for pairs of coupled excitatory neurons, while spike-triggered adaptation causes locking with a small phase difference, as long as synaptic heterogeneities are negligible. For inhibitory pairs synchrony is stable and robust against conduction delays, and adaptation can mediate bistability of in-phase and anti-phase locking. We further demonstrate that stable synchrony and bistable in/anti-phase locking of pairs carry over to synchronization and clustering of larger networks. The effects of adaptation in aEIF neurons on PRCs and network dynamics qualitatively reflect those of biophysical adaptation currents in detailed Hodgkin-Huxley-based neurons, which underscores the utility of the aEIF model for investigating the dynamical behavior of networks. Our results suggest neuronal spike frequency adaptation as a mechanism synchronizing low frequency oscillations in local excitatory networks, but indicate that inhibition rather than excitation generates coherent rhythms at higher frequencies. 相似文献
20.
To obtain different cell populations at specific cell cycle stages, we used a cell culture synchronization protocol. Effects of five different cell cycle inhibitors acting throughout the cell cycle were examined by DNA flow cytometric analysis of a synchrony/release lymphoma cell line (CEM). The screening synchronized protocol showed that staurosporine, mimosine and aphidicolin are reversible G1 phase inhibitors that act at different times. Staurosporine acted in early G1, exhibited the strongest cytotoxic effect, and induced apoptosis. Mimosine and aphidicolin acted in late G1 and at the G1/S boundary, respectively. Hydroxyurea arrested CEM cells in early S phase, but later than the aphidicolin arrest point. Nocodazole synchronized CEM cells in M phase. All the inhibitors examined in this study can be used to synchronize cells at different phases of the cell cycle and were reversible with little toxicity except for staurosporine which is highly toxic. Because the regulatory mechanism of the cell cycle is disrupted by their effects on protein synthesis, however, these drugs must be used with caution. 相似文献