安徽虫瘟霉菌株的强毒杀蚜应与侵染速率

报道安徽虫瘟霉（Ｚｏｏｐｈｔｈｏｒａ ａｎｈｕｉｅｎｓｉｓ）菌株Ｆ９７０２８对桃蚜（Ｍｙｚｕｓ ｐｅｒｓｉｃａｅ）的强毒杀蚜活性。以７个孢子剂量（０．４￣１０．４个孢子／ｍｍ＾２）接种２￣３龄若蚜（５２￣８６头／剂量）,连续观察７ｄ,所获数据经时间－剂量－死亡率模型模拟分析,接种后第３￣７ｄ的ＬＤ５０分别为３４．８、８７、１．５、０．７和０．４个孢子／ｍｍ＾２;在所有剂量范围内ＬＴ５０为２．９￣６     

中国猕猴类的蛋白多态性及其遗传分化关系

采用蛋白电泳分析技术研究了来源于我国和越南的猕猴属（Ｇｅｎ ｕｓＭａｃａｃａ）５个种（Ｍ．ｍｕｌａｔｔａ、Ｍ．ａｒｃｔｏｉｄｅｓ、Ｍ．ａｓｓａｍｅｎｓｉｓ、Ｍ．ｔｈｉｂｅｔａｎａ、Ｍ．ｆａｓｃｉｃｕｌａｒｉｓ）的蛋白多态性及其遗传分化关系。共分传座位３０个。在分析的１９只恒河猴中,３０个遗传座有位９个座位表现出多态性,多态座位百分比Ｐ＝０．３,平均等位基因数Ａ＝１．４,平均杂合度Ｈ＝０．０１０４５     

蜘蛛病原真菌一新种

虫壳属一新种大别山虫壳（Ｔｏｒｒｕｂｉｅｌａｄａｂｉｅｓｈａｎｅｎｓｉｓ）及其同时在蛛体上并存的无性型大别山球束梗孢（Ｇｉｂｅｌｕｌａｄａｂｉｅｓｈａｎｅｎｓｉｓ）。子囊壳表生至浅埋生,孔口黄色,子囊孢子弹射后呈波浪形弯曲。次生子囊孢子长柱形,９．４～１５．１×０．７～１．２μｍ。孢梗束单个或两个,白色,在其２／３处仍有分生孢子梗。分生孢子梗长２７～４４μｍ,光滑。分生孢子纺锤形,３．２～４．０×１．１～１．８μｍ。具糙梗孢式的共无性型方式,分生孢子线形,１１．０～２０．０×０．７～１．１μｍ。列出了该属已知种的检索表,并分别命以中名     

香椿的组织培养和玻璃苗的防止

１植物名称香椿（Ｔｏｏｎａｓｉｎｅｎｓｉｓ）。２材料类别（１）种子发芽３～５ｄ后的下胚轴及带少许下胚轴的子叶;（２）当年生半木质化的腋芽茎段。３培养条件芽诱导及增殖培养,以ＭＳ为基本培养基,蔗糖３０ｇ·Ｌ~（－１）,琼脂０．５％,附加激素（单位ｍｇ·Ｌ~（－１））：（１）６－ＢＡ０．２;（２）６－ＢＡ０．２、ＧＡ_３２．０;（３）ＩＡＡ０．１、６ＢＡ０．２;（４）ＺＴ０．２、ＧＡ_３２．０。诱导生根培养基为１／ＺＭＳ或仅含ＭＳ有机质（铁盐减半）,附加１．０ｍｇ·Ｌ~（－１）ＩＢＡ、１５ｇ·Ｌ~（－１）…     

缢蛏碱性磷酸酶胍溶液中分子折叠与活力变化研究

报道了缢蛏碱性磷酸酶（简称ＡＬＰ）经不同浓度盐酸胍处理时酶的分子构象所发生的变化以及酶变化和失活的动力学过程。在胍中酶荧光发射峰强度下降,紫外差光谱在２４６ｎｍ和２８５ｎｍ处出现２个负峰,ＣＤ谱中酶的α螺旋度下降,且随浓度增大,变化程度也加大。动力学研究表明,酶在０．５ｍｏｌ／Ｌ、１．０ｍｏｌ／Ｌ、２．０ｍｏｌ／Ｌ３．０ｍｏｌ／Ｌ、４．０ｍｏｌ／Ｌ盐酸胍中的变性速度常数分别为３．２１×１０~（－４）ｓ~（－１）、６．３８×１０~（－４）ｓ~（－１）、２．１７×１０~（－３）ｓ~（－１）、２．３３×１０~（－３）ｓ~９－１）、５．１７×１０~（－３）ｓ~（－１）;而酶在相应盐酸胍中的失活速度常数分别为２．３３×１０~（－４）ｓ~（－１）、３．５７×１０~（－４）ｓ~（－１）、５．８６×１０~（－４）ｓ~（－１）、１．１４×１０~（－３）ｓ~（－１）、３．４５×１０~（－３）ｓ~（－１）;表现为失活与构象伸展变化基本平行。     

滇池太湖新银鱼的生长特性与资源量关系的研究

为了滇池中太源新银鱼（ＮｅｏｓａｌａｎｘｔａｉｈｕｅｎｓｉｓＣｈｅｎ）达到稳产,高产,优质,高效的目的,管理部门急需寻求一个合理的资源平衡点,经３１个月的连续采样调查,应用ＶｏｎＢｅｒｔａｌａｎｆｆｙ生长方程,得到太湖新银鱼１９８９年和１９９０年生长方程分别为：Ｌ＝６．７０（１－ｅ＾－０．１９８（ｔ－０．２２５）,Ｗ＝１．７８（１－ｅ＾－０．１９８（ｔ－０．２２５））＾３和Ｌ＝７．４８（１－ｅ＾－     

南川升麻种子休眠与萌发的研究

南川升麻（ＣｉｍｉｃｉｆｕｇａｎａｎｃｈｕａｎｅｎｓｉｓＨｓｉａｏ）的种子自然脱落时尚处于球形胚发育阶段,需要长时间的越冬过程才能完成胚的后熟。室内采用低温湿润或不同浓度的ＧＡ３处理,可以不同程度地加快其胚的后熟过程。休眠种子用０．１ｇ／ＬＧＡ３处理１周后,在低温（１～５℃）湿润条件下存放约９０ｄ,萌发率可达到７０％以上。     

高活力胰岛素研究：B3—Lys猪胰岛素的结晶及初步晶体学分析

用化学半合成方法制备的Ｂ３—Ｌｙｓ猪胰岛素（Ｂ３ＫＰＩ）具有与天然猪胰岛素相同的体内生物活力,但其体外生物活力及受体结合活力比天然猪胰岛素高。本文报道Ｂ３ＫＰＩ的晶体生长,初步晶体学分析及衍射数据与处理。Ｂ３ＫＰＩ晶体的衍射分辨率可以达到２．１Ａ（１Ａ＝０．１ｎｍ）,空间群为Ｒ３,晶胞参数为：ａＨ＝ｂＨ＝８２．２３ＡＣＨ＝３４．１７Ａ．收集了一套２．１Ａ分辨率的衍射数据,数据完全度为８１％,Ｒｍｅｒｇｅ为２．９％。     

落叶松落叶病的生态因子及其生态模型的研究

通过１９８７－１９９１年在吉林省磐石等３个县（市１７）个林场３００余块长白落叶松（Ｌａｒｉｘ ｏｌｇｅｎｓｉｓ）人工林标准地调查、定点观察试验和统计分析表明,落叶松落叶病（Ｍｙｃｏｓｐｈａｅｒｅｌｌａ Ｌａｒｉｃｉ－ｌｅｐｔｏｌｅｐｉｓ）的林分病情指数与林分密度、林分类型、平均枝下高、土壤种类、林龄和林分地形有密切关系;对１９９０年１０３块标准地的数量化理论１分析,其偏相关系数分别为０．７２３、０     

云南纵坑切梢小蠹四个地理种群同工酶比较研究

采用不连续聚丙烯酰胺凝胶电泳技术研究了纵坑切梢小蠹（Ｔｏｍ ｉｃｕｓ ｐｉｎｉｐｅｒｄａ Ｌ．）４ 个自然种群的９ 个同工酶基因座。４ 个种群均在Ｅｓ－ １、Ｅｓ－ ２、Ｅｓ－ ４、Ｍｄｈ－ １、Ｍｄｈ－ ２ 及ＡＡＴ－ １ 基因座上存在遗传多态现象。路南长湖、楚雄、蒙自３ 种群间的遗传距离为０．００３６～０．０１７３, 平均值为０．０１０５, 表明其遗传结构基本相似。丽江种群与上述３ 种群之间的遗传距离为０．１４２１～０．２０３５, 平均值为０．１７６５,表明丽江种群与上述三种群已有了遗传分化。丽江种群近交系数较大,近亲繁殖程度较高。种群遗传结构的差异可能与不同蠹害程度之间存在一定的内在联系。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.