Evolution alpiner Populationen vonEuphrasia (Scrophulariaceae): Die mittel- bis kleinblütigen,drüsenhaarigen Arten

A more precise taxonomic concept ofE. hirtella and its infraspecific synonymy is presented. Its diploid nature (2n = 22) is confirmed. Within the European area ofE. hirtella five different races may be recognised: typical, brandisii, capitulata, Rofan and Bretagne. Taxonomic rank is not yet attributed to these races. The heterogeneous taxonomic assembly E. drosocalyx is disentangled. The type refers to products of hybrid introgression ofE. rostkoviana-characters (long glandular hairs) intoE. minima.

Former contributions of this series areEhrendorfer & Vitek (1984) andGreilhuber & al. (1984).     

A Cytogenetic Study of the Leaf Beetle Genus Cyrtonus (Coleoptera, Chrysomelidae)

The chromosomes of ten species of Cyrtonus and the genome sizes of six are surveyed. Among the total of 15 chromosomally studied species, 11 have 2n=28 chromosomes and a 13+Xy_p male meioformula, three have 2n=40 and 19+Xy_p and one 2n=46 and 22+Xy_p. All but one species with 28 chromosomes show only metacentric or submetacentric chromosomes, whereas the species with 40 and 46 chromosomes display some telocentrics or subtelocentrics, that are probably derived from the former by centric fissions. However, since the number of major chromosome arms is strikingly higher in these latter species (NF=70 and 78) than in the 28-chromosome species (mostly NF=56), other chromosomal rearrangements such as pericentric inversions or heterochromatin accretions could also be involved. The genome sizes display a narrow range, from 1C=0.6–1.22pg, and they are not significantly correlated with the chromosome numbers. Some possible factors implied in the rough chromosomal evolution of Cyrtonus are discussed in relation to a few other genera of the subfamily Chrysomelinae.     

Enzymic synthesis of the trisaccharide core region of the carbohydrate chain ofN-glycoprotein

Transmannosylation from mannotriose (Man1-4Man1-4Man) to the 4-position at the nonreducing end N-acetylglucosaminyl residue ofN,N-diacetylchitobiose was regioselectively induced through the use of -d-mannanase fromAspergillus niger. The enzyme formed the trisaccharide Man1-4GlcNAc1-4GlcNAc (3.7% of the enzyme-catalysed net decrease ofN,N-diacetylchitobiose) from mannotriose as a donor andN,N-diacetylchitobiose as an acceptor. Mannobiose (Man1-4Man) was also shown to be useful as a donor substrate for the desired trisaccharide synthesis.Abbreviations Man d-mannose - (M _n) (n=1–5) -linkedn-mer of mannose - GlcNAc₂ 2-acetamido-2-deoxy--d-glucopyranosyl-(1–4)-2-acetamido-2-deoxy-d-glucose     

Meiotic studies ofElymus nutans andE. jacquemontii (Poaceae,Triticeae) and their hybrids withPseudoroegneria spicata and seventeenElymus species

Hybridizations ofElymus nutans andE. jacquemontii were carried out with one species ofPseudoroegneria (S genome), and 20Elymus species, each containing either of the SH, SY, SYH, or SYW genomes. Chromosome configurations were analysed at metaphase I of the two target taxa and their interspecific hybrids. It is concluded that (i)E. nutans is an allohexaploid containing the SYH genomes, andE. jacquemontii is an allotetraploid having the SY genomes; (ii) the genomic affinity is associated with the geographic distance between the species studied; (iii) minor genomic structural rearrangements have occurred within the hexaploid taxon ofE. nutans.     

Pollination biology of two species ofKielmeyera (Guttiferae) from Brazilian cerrado vegetation

The pollination biology and breeding systems ofKielmeyera coriacea andK. speciosa, two sympatric woody species common in the cerrado vegetation of C. Brazil, were studied. Both species have similar nectarless, polystemonous Papaver-type flowers which are visited by a similar spectrum of insects, though they bloom in different seasons and are thus phenologically isolated. Large carpenter bees seem to be the most important pollinators and these and other bees effect buzz pollen retrieval despite the fact that anthers are not poricidal. Both species ofKielmeyera possess strong xenogamous breeding systems. The presence of staminate flowers and andromonoecy inK. coriacea, as well as the longevity ofK. speciosa flowers are discussed as alternative strategies to improve pollination success and reproductive efficacy.     

Evolution of a protein superfamily: Relationships between vertebrate lens crystallins and microorganism dormancy proteins

Summary A search of sequence databases shows that spherulin 3a, an encystment-specific protein ofPhysarum polycephalum, is probably structurally related to the - and -crystallins, vertebrate ocular lens proteins, and to Protein S, a sporulation-specific protein ofMyxococcus xanthus. The - and -crystallins have two similar domains thought to have arisen by two successive gene duplication and fusion events. Molecular modeling confirms that spherulin 3a has all the characteristics required to adopt the tertiary structure of a single -crystallin domain. The structure of spherulin 3a thus illustrates an earlier stage in the evolution of this protein superfamily. The relationship of - and -crystallins to spherulin 3a and Protein S suggests that the lens proteins were derived from an ancestor with a role in stressresponse, perhaps a response to osmotic stress.     

Development and parasitism in the genus Hemileia (uredinales)

Seventeen species ofHemileia were studied with emphasis on these characters: extent of colonization, specialization of parasitic mycelium, haustorial development, pathologic histology, soral morphology and sporulation capacity. An attempt was made to trace the varying degree of development and parasitism in this rust genus comprising of just over 40 reported species. It was noted that theGopalkrishnan's Subepidermal type more advanced than his Superstomal — B type.Fourth part of my thesis submitted to the University of Poona, India, accepted in 1968 for Ph.D. Not published.     

Requirements for in vitro propagation of seven nitrogen-fixing Alnus species

Studies on the in vitro propagation of Alnus crispa, A. glutinosa, A. incana, A. japonica, A. rubra, A. sinuata and A. viridis indicated interspecific as well as intraspecific variations in their requirements for in vitro culture. The WPM and Blaydes media supported, respectively, growth of A. glutinosa and A. crispa but not that of both species, while the MS medium induced equal or significantly better growth than WPM and Blaydes media for both species. The optimum type and concentration of sugar to be used in the multiplication medium varied with species. Only A. glutinosa showed good growth on sucrose while glucose was optimum for all other species but at different concentrations. All species rooted in 3 weeks on half-strength MS medium including 1 M IBA. All clones of A. glutinosa and A. rubra rooted 100%, whereas easy-to-root and difficult-to-root clones were observed in the other species. In the rooting medium, glucose promoted rooting of the difficult-to-root clones better than sucrose. Survival following transfer to an artificial substrate was 100% for all species. Nodulation tests using pure cultures of two Frankia strains showed 100% nodulation on all Alnus clones.     

The filtration apparatus of Cladocera: Filter mesh-sizes and their implications on food selectivity

Summary The filtering apparatus of eleven Cladoceran species was studied. The distances between the setulae, which act as filters, were measured. Among adult individuals, they vary from 0.2 m in Diaphanosoma brachyurum to 4.7 m in Sida crystallina. Species can be grouped according to the mesh-sizes, as fine mesh filter-feeders: Diaphanosoma brachyurum, Ceriodaphnia quadrangula, Chydorus sphaericus, Daphnia cucullata and Daphnia magna; medium mesh filter-feeders: Daphnia galeata, D. hyalina. D. pulicaria, Bosmina coregoni, and coarse mesh filter-feeders: Holopedium gibberum and Sida crystallina. In Daphnia hyalina, the distances between setulae increase from 0.3–0.4 m in small juveniles, to 0.8–2.0 m in adults. In Daphnia magna, the mesh-size of the filter does not increase significantly with growth. There is good evidence that the relative abundance of the filter-feeding types varies with the trophic state of the lake. In oligotrophic lakes the coarse mesh filter-feeders usually dominate throughout the year. The seasonal succession of zooplankton species in eutrophic lakes can be interpreted as a succession of feeding types; during winter coarse mesh filter-feeders dominate, while fine mesh filter-feeders are most abundant during summer phytoplankton blooms. Our results support the hypothesis that the species composition of filter-feeding zooplankton is strongly influenced by the amount of suspended bacteria which are available as food only for filter-feeding species with fine meshes.     

A preliminary phylogeny for the NeotropicalRubiaceae

The sequence of subfamilies,Cinchonoideae, Antirheoideae andRubioideae, attemps to show their natural affinities and phylogeny. The subfamilies are those ofVerdcourt, and the order in which they are presented is that ofBremekamp. A list is presented of the subfamilies, tribes and genera of theRubiaceae to be utilized in the Catálogo Ilustrado de las plantas de Cundinamarca, Colombia.     

Investigations into the inter-relationships ofPhaseolus vulgaris L. andP. Coccineus LAM

The crossability ofP. coccineus (self-incompatible) andP. vulgaris (self-compatible) was much higher whenvulgaris was used as the female parent. Reciprocal difference in crossability was due to the retarded development of embryo and endosperm tissue during the immediate post-fertilization period in the crossP. coccineus ×P. vulgaris . Backcrossing the reciprocal F₁ hybrids to the maternal species was more successful than to the paternal species.Two varieties ofP. vulgaris differed significantly in their ability to hybridize with six genotypes ofP. coccineus; this difference was shown to be genetical.The F₁ hybrids were readily distinguishable from the parent species at the seedling stage and could be classified into two distinct morphological types; one group showed and abnormal wrinkling of the leaves. The frequency of the abnormal progeny in the F₁ generation could be satisfactorily explained on the basis of either allelic or genic interaction. The importance of this genetic system in the divergence of the two species is discussed.     

On the probability of interaction between elementary radiation-induced chromosomal injuries

Summary Within the Theory of Dual Radiation Action, the heuristically useful function,(), that two energy depositions, a distance apart, will result in observable damage can be written in terms of two more fundamental quantities: One,s(), describes the structure of the sensitive matrix of the cell. The other,g(), describes the probability that two elementary injuries, a distance apart, will combine to produce observable damage. A priori, the known enhancement exhibited byy() at nanometer separations could be due to an enhancement ofs(),g() or both. For the endpoint of chromatid exchanges in Chinese hamster cells,() has been evaluated from experimental data ands() from a model system. From theseg() has been estimated and is roughly constant below 1 µm, decreasing rapidly at larger separations. Thus the enhanced short-range effect appears to be a function more of the spatial characteristics of the target rather than the probability of damage interaction.     

Characterization of the MHC class II region in cattle. The number of DQ genes varies between haplotypes

The organization of the major histocompatibility complex (MHC) class II region in cattle was investigated by Southern blot analysis using human probes corresponding to DO, DP, DQ, and DR genes. Exon-specific probes were also employed to facilitate the assessment of the number of different bovine class II genes. The results indicated the presence of single DO and DR genes, at least three DR genes, while the number of DQ genes was found to vary between MHC haplotypes. Four DQ haplotypes, DQ ^{1 1} to DQ ^{2 4}, possessed a single DQ and a single DQ gene whereas both these genes were duplicated in eight other haplotypes, DQ ^{3 5} to DQ ^{9 12}. No firm evidence for the presence of bovine DP genes was obtained. The same human probes were also used to investigate the genetic polymorphism of bovine class II genes. DQ DQ , DR DR , and DO restriction fragment length polymorphisms (RFLPs) were resolved and in particular the DQ restriction fragment patterns were highly polymorphic. Comparison of the present result with the current knowledge of the class II region in other mammalian species suggested that the DO, DP, DQ, DR, and DZ subdivision of the class II region was established already in the ancestor of mammals. The DP genes appear to be the least conserved class II genes among mammalian species and may have been lost in cattle. The degree of polymorphism of different class II genes, as revealed by RFLP analyses, shows striking similarities between species.     

Electrophoretic analysis of the high-molecular-weight glutenin subunits of Triticum monococcum,T. urartu,and the A genome of bread wheat (T. aestivum)

Summary The high molecular weight (HMW) subunit composition of glutenin was analysed by sodium dodecyl sulphate, polyacrylamide gel electrophoresis (SDS-PAGE) in the A genome of 497 diploid wheats and in 851 landraces of bread wheat. The material comprised 209 accessions of wild Triticum monococcum ssp. boeoticum from Greece, Turkey, Lebanon, Armenia, Iraq, and Iran; 132 accessions of the primitive domesticate T. monococcum ssp. monococcum from many different germplasm collections; one accession of free-threshing T. monococcum ssp. sinskajae; 155 accessions of wild T. urartu from Lebanon, Turkey, Armenia, Iraq, and Iran; and landraces of T. aestivum, mainly from the Mediterranean area and countries bordering on the Himalayan Mountains. Four novel HMW glutenin sub-units were discovered in the landraces of bread wheat, and the alleles that control them were designated Glu-Ald through Glu-Alg, respectively. The HMW subunits of T. monococcum ssp. boeoticum have a major, x subunit of slow mobility and several, less prominent, y subunits of greater mobility, all of which fall within the mobility range of HMW subunits reported for bread wheat. In T. monococcum ssp. monococcum the range of the banding patterns for HMW subunits was similar to that of ssp. boeoticum. However, two accessions, while containing y subunits were null for x subunits. The single accession of Triticum monococcum ssp. sinskajae had a banding pattern similar to that of most ssp. boeoticum and ssp. monococcum accessions. The HMW subunit banding patterns of T. urartu accessions were distinct from those of T. monococcum. All of them contained one major x and most contained one major y subunit. In the other accessions a y subunit was not expressed. The active genes for y subunits, if transferred to bread wheat, may be useful in improving bread-making quality.     

Weitere Untersuchungen an Nachkommen von Kürbis-Artbastarden

Zusammenfassung Aus 1950 durchgeführten Artkreuzungen zwischenCucurbita maxima Duch. und weichschaligemC. pepo L. wurden mittels Embryonenkultur F₁-Bastarde gewonnen. An Hand zweimaliger Rückkreuzung mitC. maxima und anschließender Selbstung gelang es, zum Teil wiederum mit Hilfe der Embryonenkultur, 4 verschiedene Bastardkombinationen bis in die F₄ zu verfolgen. In dieser Generation spalteten (1954 und 1955) bei Auswertung von 72 Familien mit insgesamt 733, davon 600 fertilen Pflanzen in 2 Bastardlinien resp. 3 Familien, insgesamt 4 weichschalige Pflanzen heraus. Da diese Pflanzen frei abblühten und das gewonnene Saatgut heterozygot war, mußten erneut zwei Selbstungsgenerationen aufgezogen werden, die in den Jahren 1956–58 mehrere erbreine Stämme weichschaliger Pflanzen lieferten.Nach Kurzer Einführung in die Anatomie der Samenschale und ihre verschiedenen Typen sowie in die bisherigen Ergebnisse genetischer Untersuchungen zur Frage der Testaausbildung wird an Hand der Spaltungsergebnisse beim erstmaligen Auftreten weichschaliger Pflanzen in der F₄ sowie der nach erneuter Selbstung in der F₆ erzielten Spaltungsverhältnisse aufgezeigt, daß das Merkmal weichschalig durchzwei Gene bedingt ist. Nach kurzer Besprechung von Spaltungen pachyspermer und leptospermer Pflanzen in der F₃ wird die Variabilität der Testaausbildung bis zum Auftreten weichschaliger Pflanzen beschrieben.In einem weiteren Kapitel wird die Variabilität in der Ausbildung der weichschaligen Testa der Bastardnachkommen, das Ergebnis der Selektion auf rein weichschalige Typen im Verlauf der 7. bis 11. Generation (1957–1964) und die Größenvariabilität der Samen der weichschaligen Typen im Vergleich zu den Ausgangsarten sowie einem Weltsortiment vonC. maxima erörtert. Zur Veranschaulichung dieser Verhältnisse wird die erstmalig vonF. Galton benutzte Methode der Contourellipsen herangezogen, deren Berechnung und zeichnerische Darstellung mit Hilfe der Großrechenanlage IBM 7090 erfolgte.Abschließend wurden die beobachtetenMendel-Spaltungen unter dem Gesichtspunkt der die Pollenmeiose sowie die Pollenentwicklung der F₁ charakterisierenden Störungen, ferner die Bedeutung des erzielten Ergebnisses für die Züchtung diskutiert. Es wird eine Formel für die Berechnung der Wahrscheinlichkeit mitgeteilt, mit der unter ähnlichen Bedingungen rezessive Pflanzen erwartet werden können, wenn das rezessive Merkmal durch n Faktoren bedingt ist und im Anschluß an die Artkreuzungj Rückkreuzungen mit der dominanten Art erforderlich sind. Auch auf die Bedeutung von Untersuchungen der vorliegenden Art für Fragen der Genlokalisation und Genanalyse wird hingewiesen.

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Further investigations on progeny of pumpkin species hybridsI. Seed formation, especially in progeny with soft seed coat, and the regularity in transmission of a recessive trait from one parental species to fertile progeny with characteristics of the other parental species

Summary Using embryo culture, we obtainedF ₁-hybrids from interspecific crosses realized in 1950 betweenCucurbita maxima Duch. and a form ofC. pepo L. with soft shell seed or weichschaligen Samen (a so called naked seed type). These and their descendants, the latter in part also obtained by embryo culture, were back-crossed toC. maxima, and theF ₃ selfed. In this way we obtained descendants from 4 different crosses between the two species.Studying (in 1954 and 1955) 72F ₄-families with a total of 733 (600 fertile) plants, we found 4 plants with soft shell seeds. They were descended from 3F ₄-families or from 2 crosses respectively between the above named species.Since these plants flowered in the presence of other progeny from our species crosses, their seed was heterozygous, and two generations of selfing were necessary to obtain (in 1956–1958) different true breeding families, whose plants furnished homozygous soft shell seed.The anatomy of seed coats and results of studies by other authors on the genetic control of different seed coat types are discussed briefly. Given the segregation ratios in theF ₄ of our soft shell seeds, as well as those obtained inF ₆ after renewed selfing, it is shown that the trait soft shell is controlled by two genes. After a short discussion of segregation in theF ₃ of pachyspermic and leptospermic plants, the variability of seed coat formation until the appearance of soft shell seed is described.In a special chapter we discuss the variability of the soft-shell seed character in hybrid progeny, the result of our selection experiments from the 7^th up to the 12^th generation (1957–1964), and the variability in the seed size of our types compared with that of pure species and of a collection ofC. maxima. To illustrate these relationships we used the method of contour-ellipses introduced byF. Galton. These ellipses were computed and designed by means of the computer IBM 7090.Finally we discuss Mendelian segregation of our plants in the light of abnormalities characteristic for meiosis of pollen-mother-cells and pollen development inF ₁, and the significance of the results to plant breeding. A formula is given for calculating the probability of obtaining recessive plants when the recessive trait depends onn factors, and whenj backcrosses to the dominant variety are necessary to establish a stable strain. The importance of experiments of this kind to the problem of gene localization and gene analysis is also pointed out.

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Herrn Professor Dr. Dr. Dr. h. c. O.Heinisch, geb. 23. 4. 1896, gest. 3. 5. 1966, zum Gedächtnis.     

Patterns and conformations of commonly occurring supersecondary structures (basic motifs) in protein data bank

Short peptides connecting-helices and-strands have been analyzed in 240 proteins refined at resolutions of 0.25 nm or better. Connecting peptides of lengths between one and five residues have been classified as part of supersecondary motifs of four types:, , , and. Careful consideration has been given to the definition of secondary structures on the basis of hydrogen bonds and main-chain conformational angles. Using five classes of residue conformation—a, b, e, l, t—in the nonregular structure regions of, space, 34 classes of supersecondary motifs occurring at least five times have been identified. Among these 34 classes, 11 classes that occur more than 25 times are commonly occurring supersecondary structure motifs. The patterns and conformations of the 11 commonly occurring supersecondary structure motifs have been characterized, demonstrating that patterns and conformations adopted by supersecondary structure motifs are limited. The results have relevance to structure prediction, comparative modeling, and protein folding.     

Existence and uniqueness of a sharp travelling wave in degenerate non-linear diffusion Fisher-KPP equations

In this paper we use a dynamical systems approach to prove the existence of a unique critical value c ^* of the speed c for which the degenerate density-dependent diffusion equation u _ct = [D(u)u _x ] _x + g(u) has: 1. no travelling wave solutions for 0 < c < c ^*, 2. a travelling wave solution u(x, t) = (x - c ^* t) of sharp type satisfying (– ) = 1, () = 0 ^*; '(^*–) = – c ^*/D'(0), '(^*+) = 0 and 3. a continuum of travelling wave solutions of monotone decreasing front type for each c > c ^*. These fronts satisfy the boundary conditions (– ) = 1, '(– ) = (+ ) = '(+ ) = 0. We illustrate our analytical results with some numerical solutions.     

Average phase difference theory and 1∶1 phase entrainment in interlimb coordination

The dynamics of coupled biological oscillators can be modeled by averaging the effects of coupling over each oscillatory cycle so that the coupling depends on the phase difference between the two oscillators and not on their specific states. Average phase difference theory claims that mode locking phenomena can be predicted by the average effects of the coupling influences. As a starting point for both empirical and theoretical investigations, Rand et al. (1988) have proposed d/dt= — K sin ), with phase-locked solutions =arcsin( /K), where is the difference between the uncoupled frequencies and K is the coupling strength. Phase-locking was evaluated in three experiments using an interlimb coordination paradigm in which a person oscillates hand-held pendulums. was controlled through length differences in the left and right pendulums. The coupled frequency _c was varied by a metronome, and scaled to the eigenfrequency _v of the coupled system K was assumed to vary inversely with _c. The results indicate that: (1) and K contribute multiplicatively to (2) =0 or = regardless of K when =0; (3) 0 or regardless of when K is large (relative to ); (4) results (1) to (3) hold identically for both in phase and antiphase coordination. The results also indicate that the relevant frequency is _c/_v rather than _c. Discussion high-lighted the significance of confirming =arcsin(/K) for more general treatments of phase-locking, such as circle map dynamics, and for the 11 phase-entrainment which characterizes biological movement systems.     

Genetics of the sex ratio anomaly in Drosophila hybrids of the Virilis group

Summary A study was made of the effect of genotype and temperature (25 and 17°C) on sex ratio in the hybrids D. virilis Sturt. X D. littoralis Sokolov. A genetic system has been found controlling sex-differential viability. In the F₁ of the reciprocal hybrids D. virilis X D. littoralis the sex ratio is normal, though at 17°C females are slightly excessive. The abnormal sex ratio is observed only in the progeny of test crosses.The major gene causing the death of female progeny of the cross [ (, D. virilis x , D. littoralis) x D. virilis] x D. littoralis is located on chromosome 2 of D. virilis. It is expressed as a lethal if chromosome 5 is heterogeneous virilis-littoralis. Chromosome 3 of D. virilis bears a modifier-enhancer and chromosome 5, a suppressor, of this lethal found in chromosome 2. This genetic system has a maternal effect and functions at 25°C, interacting with the X-chromosome of D. littoralis. If the maintainance temperature is lowered to 17°C, the progeny of the cross hybrid FB₁ x D. littoralis is predominantly female. Partial death of males is accounted for by a disturbance in the interaction between the genes of X-chromosome in certain combinations with the D. virilis autosomes and the Y-chromosome of the paternal species D. littoralis.Sex-differential mortality in the hybrids D. virilis x D. littoralis is one of the isolating factors between these species which does not appear to act until the second and subsequent F₁ generations due to the formation of the recombination load.     

The quantitative analysis of chromosome pairing and chiasma formation based on the relative frequencies of M I configurations

Whilst reliable estimates of chiasma frequencies can usually not be obtained, the probability (b) of a chromosome arm to be bound by at least one chiasma can often be determined. In the absence of interference this probability equals (1–e ^–2), where 2 is the average chiasma frequency of the chromosome arm and the average crossover frequency or map length. In the presence of interference is shown to retain its genetic meaning as an additive metric that may describe the chromosome arm or other distinctive chromosome segment in terms of genetic recombination. It is a form of potential map length, comparable to, but numerically different from the regular map length. It is termed provisionally crossing-over potential.A chromosome with armsm andn with crossing-over potentials and will form ring bivalents with a frequency (1–e ^–2).(1–e ^–2); open bivalents with a frequency (1–e ^–2).e ^–2+(1–e ^–2).e ^–2; univalent pairs with a frequencye ^–2.e ^–2. Estimates of these frequencies yield equations from which and may be solved. In rye (Secale cereale) their ratio (q) is approximately two and differs from the mitotic arm length ratio of 1.4, indicating localization of chiasmata in the long arms.Graphs are given to show how, with constantq, the relation between the probabilitiesb _m andb _n of the two arms being bound changes with changing averageb.Data are presented on chiasma frequencies in M I, and compared with the frequencies expected in the absence of interference to give an impression of the degree of interference. Apparent fusion of chiasmata simulates interference.