生物体微弱磁场检测技术在某部特殊作业人员健康评估中的应用研究

目的:研究某部特殊作业人员的健康状况,为有针对性地提出防护措施提供参考依据。方法:随机抽取某部不同工作岗位的特殊作业人员145名,进行生物体微弱磁场检测分析,以获取包括疲劳、免疫、睡眠、脑机能、血压、心脏、消化、肝胆、泌尿生殖、呼吸、运动、钙代谢、糖代谢、脂代谢、嘌呤代谢等15个系统在内的108项健康评估检测指标。对于每一项指标,仪器自带有其正常值范围,凡低于下限或高于上限的指标被视为异常。结果:所测特殊作业人员总体在钙代谢系统、消化系统、心脏系统、血压系统、呼吸系统、运动系统、免疫系统等七个系统存在不适症状的较为突出,其中钙代谢失衡的占比83.45%,脾胃不和的占比78.62%,心脏功能欠佳的占比72.41%,血压不稳的占比64.14%,咽喉不适的占比59.31%,骨关节不适的占比58.62%,免疫功能下降的占比51.72%。出现运动系统"颈椎疾患"症状的人数占比,实验组明显高于对照组(P0.05)。结论:特殊作业环境可能会影响作业人员的身体健康,应采取有效的安全防护措施,以减弱或消除有毒有害化学物质污染、强噪声、电磁辐射等对人体健康的影响。     

消化系统中的门脉系统

本文介绍了消化系统中的肝门脉系统、胆固门脉系统、小肠绒毛门脉系统系统、胰岛一外分泌门脉系统和唾液腺门脉系统的结构和功能。     

河西走廊山地-绿洲-荒漠复合系统耦合的景观生态学机制

系统耦合的基础为非生物环境的空间异质性,基本条件为具有类型相同并且相互连通的廓道,系统耦合的景观生态学机制为非生物环境的空间分异和各种干扰导致的景观异质化,各种干扰是系统耦合的介质,系统耦合从本质上可以被看作尺度变大过程中景观组分的融合与性质转换的过程,系统耦合根据干扰的性质可以分为自然耦合和人为耦合2类,自然耦合是人为耦合的基础,系统生态生产力的提高取决于人为耦合的优化过程。     

“山地-绿洲-荒漠”复合系统土地利用变化的生态系统服务权衡与协同效应——以张掖市为例

开展复合生态系统土地利用变化的生态服务效应研究可为优化国土空间格局和强化生态系统管理提供重要的基础依据。以干旱区"山地-绿洲-荒漠"复合系统的典型区域张掖市为例,基于1987年、2000年和2018年3期土地利用数据,以乡级行政区为基本单元,引入相对优势度指数划分山地、绿洲、荒漠子系统,研究复合系统的土地利用变化特征及生态系统服务权衡/协同效应。结果表明：（1）张掖市复合系统呈现地域分异特征,由南部山地系统,中部绿洲系统和北部荒漠系统构成;山地系统以林地和草地为主,绿洲系统以耕地和建设用地为主,荒漠系统以未利用地为主。（2）从时间上看,2000-2018年各系统的土地利用变化更强烈,其中,绿洲系统的耕地面积增加量最大,为363.08km²,荒漠系统的建设用地面积增加幅度最大,为78.23%。各系统土地利用转移模式为：山地系统以草地转耕地为主,绿洲系统和荒漠系统以未利用地转耕地为主。（3）张掖市复合系统的生态服务价值呈增加趋势,其中,荒漠系统生态服务价值量的增幅最大,各子系统的价值量之间的关系表现为山地系统 > 绿洲系统 > 荒漠系统,耕地对各系统生态服务价值贡献量最大。（4）张掖市土地利用变化的协同效应大于权衡效应,其中荒漠系统的生态服务之间均为协同关系,山地系统中供给服务与其他服务呈现权衡关系,占比32.73%,绿洲系统中调节服务与其他服务呈现权衡关系,占比18.18%。     

草原鼢鼠土丘与不同根系类型植物的关系

本研究于2013-2015年的5月（春季）和9月（秋季）,在锡林浩特市南部白银库伦牧场的天然草地监测草原鼢鼠（Myospalax aspalax）生境中植被情况及新旧土丘数量,采用偏冗余分析的方法研究了新旧土丘数量与植物地上生物量的干重、株丛数和物种数之间的关系。结果表明：5月鼢鼠新土丘数量与轴根型、根蘖型植物呈正相关且具有较大解释量。鼢鼠旧土丘数量与疏丛型植物呈正相关,与轴根型和根蘖型植物呈负相关,且具有较大解释量。9月鼢鼠新土丘数量与疏丛型、密丛型和根蘖型植物呈正相关且解释量较大。鼢鼠旧土丘与疏丛型、密丛型和根蘖型植物呈正相关且解释量较大。这些结果说明草原鼢鼠的造丘活动和微栖息地选择和食物资源有关,存在季节差异。轴根型和根蘖型植物对鼢鼠的春季活动有重要影响;疏丛型、密丛型和根蘖型植物对鼢鼠的秋季活动有重要影响。     

部分国家和地区分级诊疗特点及启示

通过剖析部分国家和地区分级诊疗的特点,即分级诊疗是医疗卫生服务体系健全的自然结果,分级诊疗的实质在于服务整合,合理的支付制度是分级诊疗的重要促进因素;提出以强基层、建机制的原则发展分级诊疗,将医疗机构分工合作、服务功能整合作为分级诊疗的重要路径,增强医保对医疗服务体系的影响力以促进分级诊疗等建议。     

国家重点生态功能区生态-经济-社会系统耦合协调的动态演化——以新疆阿勒泰地区为例

探究国家重点生态功能区生态-经济-社会系统耦合协调的演化特征,对实现可持续发展目标、建设美丽中国有重要意义。运用耦合协调模型、剪刀差方法、耦合度模型以及VAR模型对2007—2019年阿勒泰地区EES系统耦合协调度的动态演化进行分析。结果表明：(1)2007—2019年阿勒泰地区整体耦合协调度呈现不断上升趋势,由2007年的中度失调(0.271)向2019年的轻度失调(0.371)演进,生态系统与经济系统、社会系统的发展水平呈现“X”型变动趋势,经济系统与社会系统持续上升,而生态系统略微下降。(2)经济系统与社会系统、生态系统与社会系统演化速率的剪刀差在2014年以后出现较大幅度的波动,而生态系统与经济系统演化速率的剪刀差趋于稳定态势,生态系统与经济系统、经济系统与社会系统的耦合度呈现出由无序到有序的初始过渡。(3)由脉冲响应函数及方差分解可得,阿勒泰地区EES系统耦合协调水平的提升是三系统共同作用的结果,前期主要由社会系统、经济系统驱动,后期主要依赖生态系统的改善。研究表明：国家重点生态功能区应着重减轻经济系统对社会系统和生态系统的胁迫强度,进而实现三者的协同发展。     

Co-expression of a prophage system and a plasmid system in Bacillus subtilis

A dual expression system for overexpressing two proteins by a single cell strain has been developed in Bacillus subtilis. This dual expression system combines the phi105MU331 prophage system and a plasmid system within a single cell. Protein expression by the prophage system is heat inducible, while that of the plasmid system is constitutive. Three candidate genes, BPN, BT, and amyE, all of Bacillus origin, were used as test models. Seven strains (BPN, BT, AMY, BS168K, MU331K, BPNK, and BTK) were constructed to investigate the influences of the prophage system and the plasmid system on each other, and to compare the efficiency of the individual expression systems with that of the dual expression system. Individually, the yield of the plasmid system is higher than that of the prophage system, which could be attributed to the constitutive nature of the expression of the plasmid system. Nonetheless, for the dual expression strains, the expression of two enzymes in a single fermentation run can reduce costs in facilities, manpower, and utilities. Fed-batch fermentation of BPNK strains confirmed the feasibility of applying this dual expression system in industrial-scale production.     

A Switching Controller System for a Wheeled Mobile Robot

A wheeled mobile mechanism with a passive and/or active linkage mechanism for rough terrain environment is developed and evaluated. The wheeled mobile mechanism which has high mobility in rough terrain needs sophisticated system to adapt various environments.We focus on the development of a switching controller system for wheeled mobile robots in rough terrain. This system consists of two sub-systems: an environment recognition system using link angles and an adaptive control system. In the environment recognition system, we introduce a Self-Organizing Map (SOM) for clustering link angles. In the adaptive controllers, we introduce neural networks to calculate the inverse model of the wheeled mobile robot.The environment recognition system can recognize the environment in which the robot travels, and the adjustable controllers are tuned by experimental results for each environment. The dual sub-system switching controller system is experimentally evaluated. The system recognizes its environment and adapts by switching the adjustable controllers. This system demonstrates superior performance to a well-tuned single PID controller.     

旅游环境系统研究进展与展望

旅游环境系统是旅游发展的基础,其协调性与完整性对旅游目的地的可持续发展具有重要推动作用。旅游环境系统具有复杂性、动态性与综合性,对人地关系的关注与研究为改善旅游环境系统的功能提供了科学依据,对旅游环境系统的研究体现了众多学者对旅游可持续发展领域达成的科学共识。文章从旅游环境系统的内涵、指标体系、量化模型、管理工具、应用研究等角度,对国内外学者的旅游环境系统的研究现状分析;根据其研究的不足,提出旅游环境系统研究展望,以期对提升旅游环境系统质量的进一步研究提供理论参考。     

Evaluation of Radiometric System for Detecting Bacteremia

An automated radiometric system (BACTEC, Johnston Laboratories) for detection of bacteremia was evaluated in parallel with a standard blood culture system in use in our laboratory. Of 1,445 blood cultures from 484 patients with possible bacteremia, 106 sets of cultures (excluding 39 presumed contaminated), representing 56 patients, were positive by both methods. The conventional system yielded 85 positive cultures from 48 patients, whereas the BACTEC system yielded 84 positive cultures from 43 patients. The BACTEC system failed to detect 22 cultures that were positive in the conventional system, and the conventional system failed to detect 21 cultures that were positive in the BACTEC system. The detection efficiency was generally equivalent in the two systems except for the lower detection rates of anaerobes and Enterobacter aerogenes by the BACTEC system and the lower detection rates of Torulopsis glabrata and, possibly, Pseudomonas sp. (group IVD) in the conventional system. The BACTEC system had a slight advantage over the conventional system in the time interval to detection of positivity. Approximately 20% of the positive cultures detected by the BACTEC system were detected on the first day of incubation compared with 7% by the conventional system. The recovery rates and detection times of anaerobes were less efficient by the BACTEC system than by the conventional system. It does not appear that the radiometric method has much advantage over available conventional methods.     

新型冠状病毒与系统损伤研究

自2019年12月全国及世界范围爆发了新型冠状病毒性肺炎(corona virus disease 2019,COVID-19),给中国和全球公共卫生安全带来了极大的挑战.研究发现,新型冠状病毒(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)不仅损...     

二种群生态系统的能量结构分析

每一个生命系统均存在一个能量系统与之相对应。能量进入生命系统后按生理的组织和功能进行分配,形成具有特定的能量传递关系,组分配置关系及相互关联形式的能量结构,这种结构是完成一定生命功能的物质基础,也是不同生命系统之间相互区别的重要特征。以二种群生态系统分析为例,从其能量的传输类型,配置和奇点的控制特性三个方面扼要地介绍了生命能量系统结构的概念和分析方法。     

门诊预约服务体系建设中的问题与对策

门诊预约服务在政府的倡导下广泛开展,门诊预约服务体系的建设是现阶段的主要任务。预约体系包括信息系统、服务系统、管理系统和评价系统,各系统相互配合构成统一的整体。目前预约服务还面临着不少难题,对不同系统的主要问题和解决思路作一简要探讨。     

中国兽类资源数据库管理信息系统的建立与应用

本文阐明了建立兽类资源管理系统的意义,然后详细介绍系统的设计目标、数据结构及系统软件的设计。介绍了标本、物种分布名录及文献库管理的功能,并对本系统创建及应用的物种、地名代码体系作了介绍。最后介绍了已经用此系统建立的昆明动物研究所兽类标本及中国兽类分布名录等基础数据库的概况。     

Optimal control mode of a biochemical feedback system

An optimal feedback system for constant-value control of biochemical reaction system was investigated by computer simulations. A feedback system containing a cyclic enzyme system where two enzyme types share a substrate in a cyclic manner, was found to be the most reliable one. This feedback system has a capability to keep the stationary value of the end product at a desired level against not only exogenous substrate supply but also endogenous parametric disturbances. The cyclic enzyme system installed as a control element of this feedback system played the role of comparator in this feedback system. The control mode of this feedback system was in good agreement with that of a system established by means of optimization technique based on the maximum principle. Also bang - bang control could be performed in this biochemical feedback system as well as in electrical one.     

植物根系构型原位观测识别技术研究进展

根系是植物从土壤中获取养分和水分并输送给植物体的重要器官,由于土壤的不透明属性阻碍了根系的观测,缺乏有效的原位根系观测方法已经构成了对植物根系深入研究的技术瓶颈.本文首先从根系研究的技术需求出发,对破坏性根系研究法、非破坏性根系二维观测法(微根管法)和根系三维构型原位观测方法如X射线计算机层析成像( X-ray computed tomography,XCT)、核磁共振成像法(magnetic resonance imaging,MRI)等进行了综述,并总结了各自的优缺点.其次,重点对XCT和MRI法进行了比较,有研究表明,MRI法不适用于根系三维构型的观测.指出自然土壤介质下,XCT是根系三维构型定量化分析未来的发展趋势.     

Characterization of a transport system for anionic amino acids in human fibroblast lysosomes

L-Aspartate and L-glutamate are transported into human fibroblast lysosomes by a single, low Km, Na(+)-independent transport system, which has been provisionally named lysosomal system d. This system resembles the Na(+)-dependent plasma membrane system chi-AG, although these differences have been observed: (1) lysosomal system d recognizes the D- as well as the L-isomers of both aspartate and glutamate, whereas only for aspartate is the D-isomer recognized by system chi-AG; (2) the anion L-homocysteate is not accepted by system chi-AG, but is an effective inhibitor of lysosomal system d; (3) N-methyl, alpha-methyl, and omega-hydroxamate derivatives of both aspartate and glutamate inhibit lysosomal system d, but only the aspartate derivatives are accepted by system chi-AG; (4) lysosomal system d shows a preference for the substrate amino group in the alpha-position, a preference not seen for system chi-AG. These points imply differences at the two recognition sites with respect to substrate length, size, and rotation, with the lysosomal site generally being the less restrictive.     

不同消化方法对测定生物样品中硒含量的比较研究

本文比较了干法消化、普通湿法消化、高压消化、微波消化对测定富含有机硒的生物样品中硒含量的影响.结果显示,高压及微波消化等密闭消化体系具有显著的优点,适合生物样品中富含有机硒的硒含量的测定.     

Improving cell-free protein synthesis for stable-isotope labeling

Cell-free protein synthesis is suitable for stable-isotope labeling of proteins for NMR analysis. The Escherichia coli cell-free system containing potassium acetate for efficient translation (KOAc system) is usually used for stable-isotope labeling, although it is less productive than other systems. A system containing a high concentration of potassium l-glutamate (l-Glu system), instead of potassium acetate, is highly productive, but cannot be used for stable-isotope labeling of Glu residues. In this study, we have developed a new cell-free system that uses potassium d-glutamate (d-Glu system). The productivity of the d-Glu system is approximately twice that of the KOAc system. The cross peak intensities in the ¹H–¹⁵N HSQC spectrum of the uniformly stable-isotope labeled Ras protein, prepared with the d-Glu system, were similar to those obtained with the KOAc system, except that the Asp intensities were much higher for the protein produced with the d-Glu system. These results indicate that the d-Glu system is a highly productive cell-free system that is especially useful for stable-isotope labeling of proteins. Electronic Supplementary Material The online version of this article (doi: ) contains supplementary material, which is available to authorized users.