共查询到20条相似文献,搜索用时 15 毫秒
1.
The baker's yeast process was optimised with a fuzzy logic controller, which is capable of detecting (with the respiratory quotient as indicator) and eliminating overdosage. The controller was developed to enable automatic modification of the set value for the respiratory quotient according to glucose concentration in the broth. With this controller, a cell yield of 55% (w/w) from glucose and a maximum specific growth rate of 0.16 h–1 were obtained. 相似文献
2.
Fuzzy control of ethanol concentration its application to maximum glutathione production in yeast fed-batch culture 总被引:1,自引:0,他引:1
Alfafara CG Miura K Shimizu H Shioya S Suga K Suzuki K 《Biotechnology and bioengineering》1993,41(4):493-501
A fuzzy logic controller (FLC) for the control of ethanol concentration was developed and utilized to realize the maximum production of glutathione (GSH) in yeast fedbatch culture. A conventional fuzzy controller, which uses the control error and its rate of change in the premise part of the linguistic rules, worked well when the initial error of ethanol concentration was small. However, when the initial error was large, controller overreaction resulted in an overshoot.An improved fuzzy controller was obtained to avoid controller overreaction by diagnostic determination of "glucose emergency states" (i.e., glucose accumulation or deficiency), and then appropriate emergency control action was obtained by the use of weight coefficients and modification of linguistic rules to decrease the overreaction of the controller when the fermentation was in the emergency state. The improved fuzzy controller was able to control a constant ethanol concentration under conditions of large initial error.The improved fuzzy control system was used in the GSH production phase of the optimal operation to indirectly control the specific growth rate mu to its critical value mu(c). In the GSH production phase of the fed-batch culture, the optimal solution was to control mu to mu(c) in order to maintain a maximum specific GSH production rate. The value of mu(c) also coincided with the critical specific growth rate at which no ethanol formation occurs. Therefore, the control of mu to mu(c) could be done indirectly by maintaining a constant ethanol concentration, that is, zero net ethanol formation, through proper manipulation of the glucose feed rate. Maximum production of GSH was realized using the developed FLC; maximum production was a consequence of the substrate feeding strategy and cysteine addition, and the FLC was a simple way to realize the strategy. (c) 1993 John Wiley & Sons, Inc. 相似文献
3.
Kazuo Dairaku Yoshihiko Yamasaki Kazuo Kuki Suteaki Shioya Takeichiro Takamatsu 《Biotechnology and bioengineering》1981,23(9):2069-2081
A feedback control system of the glucose feed rate in a bakers' yeast fed-batch culture was developed by keeping the ethanol concentration constant. A PID controller and on–off controller were applied and discussed with the aid of the porous Teflon tubing method. Experimental results showed the effectiveness of the control system for avoiding the glucose effect and glucose starvation. It was shown that the feedback control system developed hare could achieve a maximum specific growth rate of 0.3 h?1 or a maximum cell yield of 0.5 g cell/g glucose in the fedhyphen;batch culture. 相似文献
4.
The biokinetics of glucose metabolism were evaluated in Aeromonas hydrophila during growth in an anaerobic biosystem. After approx 34 h growth, A. hydrophila metabolized 5,000 mg glucose l−1 into the end-products ethanol, acetate, succinate and formate. The maximum growth rate, μ
m, half saturation coefficients, K
s, microbial yield coefficient, Y, cell mass decay rate coefficient, k
d, and substrate inhibition coefficient, K
si were 0.25 ± 0.03 h−1, 118 ± 31 mg glucose l−1, 0.12 μg DNA mg glucose−1, 0.01 h−1, and 3,108 ± 1,152 mg glucose l−1, respectively. These data were used to predict the performance of a continuous growth system with an influent glucose concentration
of 5,000 mg l−1. Results of the analysis suggest that A. hydrophila will metabolize glucose at greater than 95% efficiency when hydraulic retention times (HRTs) exceed 7 h, whereas the culture
is at risk of washing out at an HRT of 6.7 h. 相似文献
5.
Jyoti B.D. Suresh A.K. Venkatesh K.V. 《World journal of microbiology & biotechnology》2003,19(5):509-514
Lactobacillus rhamnosus is a heterolactic acid bacterium, which can be used to produce flavour compounds like diacetyl and acetoin. Various startegies have been applied to improve the growth rate and diacetyl yield. The use of multiple substrates affected growth as well as the yield of diacetyl. Growth on a medium containing glucose demonstrated a diauxic growth profile, with the second phase of growth being on the product, lactic acid. L. rhamnosus also grew on a medium containing citrate. Growth on medium containing glucose+citrate demonstrated simultaneous utilization of carbon sources. L. rhamnosus did not grow in a medium containing acetate and also did not co-metabolize it with glucose. Maximum specific growth rate (
max) was found to increase in the case of simultaneous utilization of glucose+citrate (0.38 h–1) as compared to glucose as the sole carbon source (0.28 h–1). The yields of diacetyl were also found to increase for glucose + pyruvate and glucose + citrate (0.10 and 0.05 g g–1 of glucose, respectively) as compared to glucose alone (0.01 g g–1 of glucose). The productivity of diacetyl on medium containing glucose and citrate was double that of a medium containing only citrate, although the yields were comparable. 相似文献
6.
Beneckea natriegens oxidizes sodium formate constitutively when grown on glucose or glycerol in chemostat culture, but cannot utilize formate as the sole source of carbon and energy for growth. However, when grown on a mixture of glucose and formate (D=0.37 h-1, pH 7.6) the yield is higher than on glucose alone.The yield, expressed in terms of g bacterial dry weight g-1 glucose plus formate carbon utilized, gave a linear relationship when plotted against the total heat of combustion of glucose plus formate utilized. Extrapolation of the plot cut the abscissa at a value equivalent to the heat of combustion of formate, which suggests that formate is not utilised as a source of carbon but only energy.In cultures with nitrate as the sole source of nitrogen the yield from glucose was lower than that observed with ammonia but the addition of formate to the culture utilizing nitrate resulted in an increase in the yield from glucose to a value similar to that observed with ammonia.At a culture pH value of 7.65 unused formate (<0.15–227 mM) in the culture supernatant had no effect on respiration spiration or yield, but at a culture pH of 6.7 excess formate caused a marked increase in respiration rate and a large decrease in the yield from glucose; further decrease in the pH value caused washout of the culture. This may be explained by undissociated formic acid causing uncoupling of oxidative phosphorylation. 相似文献
7.
The steady-state residual glucose concentrations in aerobic chemostat cultures of Saccharomyces cerevisiae ATCC 4126, grown in a complex medium, increased sharply in the respiro-fermentative region, suggesting a large increase in the apparent ks value. By contrast, strain CBS 8066 exhibited much lower steady-state residual glucose concentrations in this region. Glucose transport assays were conducted with these strains to determine the relationship between transport kinetics and sugar assimilation. With strain CBS 8066, a high-affinity glucose uptake system was evident up to a dilution rate of 0.41 h–1, with a low-affinity uptake system and high residual glucose levels only evident at the higher dilution rates. With strain ATCC 4126, the high-affinity uptake system was present up to a dilution rate of about 0.38 h–1, but a low-affinity uptake system was discerned already from a dilution rate of 0.27 h–1, which coincided with the sharp increase in the residual glucose concentration. Neither of the above yeast strains had an absolute vitamin requirement for aerobic growth. Nevertheless, in the same medium supplemented with vitamins, no low-affinity uptake system was evident in cells of strain ATCC 4126 even at high dilution rates and the steady-state residual glucose concentration was much lower. The shift in the relative proportions of the high and low-affinity uptake systems of strain ATCC 4126, which might have been mediated by an inositol deficiency through its effect on the cell membrane, may offer an explanation for the unusually high steady-state residual glucose concentrations observed at dilution rates above 52% of the wash-out dilution rate. 相似文献
8.
Plasma glucose kinetics and tissue uptake in brown trout in vivo: effect of an intravascular glucose load 总被引:2,自引:0,他引:2
J. Blasco J. Fernàndez-Borràs I. Marimon A. Requena 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1996,165(7):534-541
The object of the present study was to elucidate whether a glucose load modifies glucose uptake by tissues in brown trout in vivo. By the use of 2-[1,2-3H]-deoxyglucose, plasma glucose disappearance rate and tissue glucose uptake were measured after an intraaortic glucose load of 500 mg·kg-1 (glucose load group) and under normoglycemic conditions (control). We also attempted to determine whether fasting modifies the glucose load disposal (fasted glucose load group). The procedure used to calculate 2-deoxyglucose uptake by tissues was evaluated, and the levels of 2-deoxyglucose uptake were compared with those of 2-deoxyglucose phosphorylation. Uptake and phosphorylation rates were similar in all tissues, except in brain and heart. In all the groups glucose uptake rates were highest in spleen, kidney, brain and gills, and lowest in red muscle, heart and white muscle. However, white muscle was the main site of glucose uptake on a whole tissue basis. The glucose load led to strong, long-lasting hyperglycemia, in spite of the increases observed in plasma insulin levels and in glucose uptake rate by the whole body (control: 4.9 mol·min-1·kg-1; glucose load group: 6.5 mol·min-1·kg-1). This higher rate was due to the higher glucose uptake only in white and red muscles (four- and threefold, respectively). Fasting halved the uptake of glucose by both red and white muscles in the load condition. In consequence the use of exogenous glucose decreased with fasting (fasted glucose load group: 5.1 mol·min-1·kg-1), causing still longer hyperglycemia.Abbreviations
bw
body weight
-
2DG
2-[1,2-3H]-deoxyglucose
-
2DG-P
2-[1,2-3H]-deoxyglucose phosphate
-
dpm
disintegrations per min
-
FGL
fasted glucose load group
-
GL
glucose load group
-
G-6-Pase
glucose-6-phosphatase
-
LG
L-[1-14C]-glucose
-
MS-222
3-aminobenzoic acid ethyl ester methanesulphonate salt 相似文献
9.
Ki Moon Park Min Woo Song Jae Heung Lee 《Biotechnology and Bioprocess Engineering》2008,13(2):197-203
Batch culture kinetics of the red yeast, Xanthophyllomyces dendrorhous SKKU 0107, revealed reduction in biomass with glucose and lower intracellular carotenoid content with fructose. Figures were
different when compared to sucrose, which is a disaccharide of glucose and fructose. In contrast, specific growth rate constant
stayed between 0.094~0.098 h−1, irrespective of the carbon sources employed. Although the uptake rate of glucose was found to be 2.9-fold faster than that
of fructose, sucrose was found to be a more suitable carbon source for the production of carotenoids by the studied strain.
When sugar cane molasses was used, both the specific growth rate constant and the intracellular carotenoid content decreased
by 27 and 17%, respectively. Compared with the batch culture using 28 g/L sugar cane molasses, fed-batch culture with the
same strain resulted in a 1.45-fold higher cell yield together with a similar level of carotenoid content in X. dendrorhous SKKU 0107. 相似文献
10.
Summary The insecticidal crystal protein (ICP) synthesized at the onset of sporulation by Bacillus thuringiensis var. galleriae (Btg) is lethal against specific pests. Attempts were made to enhance the synthesis of biomass and ICP by Btg employing process optimization strategies. The process optimization was carried out with residual glucose concentration control in a bench scale bioreactor. A fuzzy logic-based feedback control system for maintaining the residual glucose concentration at a constant level during cultivation was developed in LabVIEW. This control system indicated the possibilities in providing a balanced substrate flux during cultivation. The identified optimum level of 2.72 g/l in residual glucose concentration was maintained by fed-batch cultivation with glucose and yeast extract fed at equal concentration with the above control system. High cell density of 16.0 g/l with specific growth rate of 0.69 h-1 was obtained during the cultivation. The balanced flux of substrate during cultivation has resulted in the enhanced synthesis of biomass and ICP. This optimized process could be commercially exploited by comparing the fluxes of basal compounds in different media sources used in fermentation. 相似文献
11.
Klebsiella aerogenes NCTC418 was cultured anaerobically under glucose-limited conditions in chemostat cultures at various growth rates, ranging from 0.13 h-1 to 0.82 h-1. It was found that the specific uptake rate of glucose varied linearly with the growth rate and that under these conditions glucose was fermented solely to acetate and ethanol plus CO2+H2 and formate.When steady-state cultures were pulsed with cell saturating concentrations of glucose, the specific glucose aptake rate increased immediately and substantially. However, at steady-state growth rates lower than 0.5 h-1, this increase was not accompanied by a change in the growth rate, in contrast to cultures growing at higher rates. It was found that relief of the glucose limitation resulted in a shift in fermentation pattern: at the lower growth rates 50% or more of the extra glucose taken up was fermented to D-lactate.Incubation experiments with sonified cells revcaled that K. aerogenes possessed all the enzymes needed to convert dihydroxyacetone phosphate to methylglyoxal and subsequently to D-lactate, and that the rate at which this overall conversion occurred in vitro was in close agreement with the production rate of D-lactate in vivo. Moreover, it was found that the activities of the enzymes of the methylglyoxal bypass were dependent on the imposed growth rate. At higher growth rates, where cells possessed the potential to increase their growth rate immediately, the activity of methylglyoxal synthase was relatively low.it could be shown that, under low growth rate conditions, the uncoupling effect of the methylglyoxal bypass was highly effective and that, as a consequence thereof, a significant increase in the uptake rate of the energy source was accompanied by only a marginal increase in the rate at which ATP was synthesized. 相似文献
12.
The effect of growth rate on the physiology of Beneckea natriegens was studied in chemostat culture. The molar growth yields (Y) from glucose and oxygen, the specific rates of oxygen (q
O
2) and glucose (q
glc) consumption and the specific rate of CO2 production (q
CO
2) were linearly dependent on the growth rate over the dilution rate 0.17 h-1 to 0.60 h-1. Further increase in the dilution rate resulted in a decrease in growth yield and respiration rate and these changes were coincident with increases in the specific rate of glucose utilisation and of acetate production. The affinity of Beneckea natriegens for glucose was similar when measured either directly in chemostat culture or in a closed oxygen electrode system using harvested bacteria. The total content of cytochromes decreased with increasing growth rate. However, the quantity of CO-binding cytochromes remained independent of growth rate and correlated with the potential respiration rate. 相似文献
13.
Yonggang Wang Peng Du Renbao Gan Zhimin Li Qin Ye 《Biotechnology and Bioprocess Engineering》2005,10(2):149-154
Secretion of the expressed heterologous proteins can reduce the stress to the host cells and is beneficial to their recovery
and purification. In this study, fed-batch cultures ofEscherichia coliYK537 (pAET-8) were conducted in a 5-L fermentor for the secretory production of human epidermal growth factor (hEGF) whose
expression, was under the control of alkaline phosphatase promoter. The effects of feeding of glucose and complex nitrogen
sources on hEGF production were investigated. When the fed-batch culture was conducted in a chemically defined medium, the
cell density was 9.68 g/L and the secreted hEGF was 44.7 mg/L in a period of 60 h. When a complex medium was used and glucose
was added in pH-stat mode, the secreted hEGF was improved to 345 mg/L. When the culture was fed with glucose at a constant
specific rate of 0.25 gg−1h−1, hEGF reached 514 mg/L. The effects of adding a solution containing yeast extract and tryptone were further studied. Different
rate of the nitrogen source feeding resulted in different levels of phosphate and acetic acid formation, thus affected hEGF
expression. At the optimal feeding rate, hEGF production achieved 686 mg/L. 相似文献
14.
Gluconobacter oxydans was grown successively in glucose and nitrogen-limited chemostat cultures. Construction of mass balances of organisms growing at increasing dilution rates in glucose-limited cultures, at pH 5.5, revealed a major shift from extensive glucose metabolism via the pentose phosphate pathway to the direct pathway of glucose oxidation yielding gluconic acid. Thus, whereas carbon dioxide production from glucose accounted for 49.4% of the carbon input at a dilution rate (D)=0.05 h-1, it accounted for only 1.3% at D=0.26 h-1. This decline in pentose phosphate pathway activity resulted in decreasing molar growth yields on glucose. At dilution rates of 0.05 h-1 and 0.26 h-1 molar growth yields of 19.5 g/mol and 3.2 g/mol, respectively, were obtained. Increase of the steady state glucose concentration in nitrogen-limited chemostat cultures maintained at a constant dilution rate also resulted in a decreased flow of carbon through the pentose phosphate pathway. Above a threshold value of 15–20 mM glucose in the culture, pentose phosphate pathway activity almost completely inhibited. In G. oxydans the coupling between energy generation and growth was very inefficient; yield values obtained at various dilution rates varied between 0.8–3.4 g/cells synthesized per 0.5 mol of oxygen consumed. 相似文献
15.
A family of 10 competing, unstructured models has been developed to model cell growth, substrate consumption, and product formation of the pyruvate producing strain Escherichia coli YYC202 ldhA::Kan strain used in fed-batch processes. The strain is completely blocked in its ability to convert pyruvate into acetyl-CoA or acetate (using glucose as the carbon source) resulting in an acetate auxotrophy during growth in glucose minimal medium. Parameter estimation was carried out using data from fed-batch fermentation performed at constant glucose feed rates of
qVG=10 mL h–1. Acetate was fed according to the previously developed feeding strategy. While the model identification was realized by least-square fit, the model discrimination was based on the model selection criterion (MSC). The validation of model parameters was performed applying data from two different fed-batch experiments with glucose feed rate
qVG=20 and 30 mL h–1, respectively. Consequently, the most suitable model was identified that reflected the pyruvate and biomass curves adequately by considering a pyruvate inhibited growth (Jerusalimsky approach) and pyruvate inhibited product formation (described by modified Luedeking–Piret/Levenspiel term).List of symbols cA
acetate concentration (g L–1)
- cA,0
acetate concentration in the feed (g L–1)
- cG
glucose concentration (g L–1)
- cG,0
glucose concentration in the feed (g L–1)
- cP
pyruvate concentration (g L–1)
- cP,max
critical pyruvate concentration above which reaction cannot proceed (g L–1)
- cX
biomass concentration (g L–1)
- KI
inhibition constant for pyruvate production (g L–1)
- KIA
inhibition constant for biomass growth on acetate (g L–1)
- KP
saturation constant for pyruvate production (g L–1)
- KP
inhibition constant of Jerusalimsky (g L–1)
- KSA
Monod growth constant for acetate (g L–1)
- KSG
Monod growth constant for glucose (g L–1)
- mA
maintenance coefficient for growth on acetate (g g–1 h–1)
- mG
maintenance coefficient for growth on glucose (g g–1 h–1)
- n
constant of extended Monod kinetics (Levenspiel) (–)
- qV
volumetric flow rate (L h–1)
- qVA
volumetric flow rate of acetate (L h–1)
- qVG
volumetric flow rate of glucose (L h–1)
- rA
specific rate of acetate consumption (g g–1 h–1)
- rG
specific rate of glucose consumption (g g–1 h–1)
- rP
specific rate of pyruvate production (g g–1 h–1)
- rP,max
maximum specific rate of pyruvate production (g g–1 h–1)
- t
time (h)
- V
reaction (broth) volume (L)
- YP/G
yield coefficient pyruvate from glucose (g g–1)
- YX/A
yield coefficient biomass from acetate (g g–1)
- YX/A,max
maximum yield coefficient biomass from acetate (g g–1)
- YX/G
yield coefficient biomass from glucose (g g–1)
- YX/G,max
maximum yield coefficient biomass from glucose (g g–1)
-
growth associated product formation coefficient (g g–1)
-
non-growth associated product formation coefficient (g g–1 h–1)
-
specific growth rate (h–1)
- max
maximum specific growth rate (h–1) 相似文献
16.
The lac operon of Escherichia coli can exhibit bistability. Early studies showed that bistability occurs during growth on TMG/succinate and lactose + glucose,
but not during growth on lactose. More recently, studies with lacGFP-transfected cells show bistability during growth on TMG/succinate,
but not during growth on lactose and lactose + glucose. In the literature, these results are invariably attributed to variations
in the destabilizing effect of the positive feedback generated by induction. Specifically, during growth on TMG/succinate,
lac induction generates strong positive feedback because the permease stimulates the accumulation of intracellular TMG, which
in turn, promotes the synthesis of even more permease. This positive feedback is attenuated during growth on lactose because
hydrolysis of intracellular lactose by β-galactosidase suppresses the stimulatory effect of the permease. It is attenuated even more during growth on lactose + glucose
because glucose inhibits the uptake of lactose. But it is clear that the stabilizing effect of dilution also changes dramatically
as a function of the medium composition. For instance, during growth on TMG/succinate, the dilution rate of lac permease is proportional to its activity, e, because the specific growth rate is independent of e (it is completely determined by the concentration of succinate). However, during growth on lactose, the dilution rate of
the permease is proportional to e
2 because the specific growth rate is proportional to the specific lactose uptake rate, which in turn, proportional to e. We show that: (a) This dependence on e
2 creates such a strong stabilizing effect that bistability is virtually impossible during growth on lactose, even in the face
of the intense positive feedback generated by induction. (b) This stabilizing effect is weakened during growth on lactose
+ glucose because the specific growth rate on glucose is independent of e, so that the dilution rate once again contains a term that is proportional to e. These results imply that the lac operon is much more prone to bistability if the medium contains carbon sources that cannot be metabolized by the lac enzymes, e.g., succinate during growth on TMG/succinate and glucose during growth on lactose + glucose. We discuss the experimental
data in the light of these results. 相似文献
17.
Glucose repressed xylose utilization inCandida tropicalis pre-grown on xylose until glucose reached approximately 0–5 g l–1. In fermentations consisting of xylose (93 g l–1) and glucose (47 g l–1), xylitol was produced with a yield of 0.65 g g–1 and a specific rate of 0.09 g g–1 h–1, and high concentrations of ethanol were also produced (25 g l–1). If the initial glucose was decreased to 8 g l–1, the xylitol yield (0.79 g g–1) and specific rate (0.24 g g–1 h–1) increased with little ethanol formation (<5 g l–1). To minimize glucose repression, batch fermentations were performed using an aerobic, glucose growth phase followed by xylitol production. Xylitol was produced under O2 limited and anaerobic conditions, but the specific production rate was higher under O2 limited conditions (0.1–0.4 vs. 0.03 g g–1 h–1). On-line analysis of the respiratory quotient defined the time of xylose reductase induction. 相似文献
18.
Continuous ethanol fermentation by immobilized whole cells ofZymomonas mobilis was investigated in an expanded bed bioreactor and in a continuous stirred tank reactor at glucose concentrations of 100, 150 and 200 g L–1. The effect of different dilution rates on ethanol production by immobilized whole cells ofZymomonas mobilis was studied in both reactors. The maximum ethanol productivity attained was 21 g L–1 h–1 at a dilution rate of 0.36 h–1 with 150 g glucose L–1 in the continuous expanded bed bioreactor. The conversion of glucose to ethanol was independent of the glucose concentration in both reactors. 相似文献
19.
A strain of Nannochloropsis isolated originally from the East China Sea and obtained from Institute of Hydrobiology, Chinese Academy of Sciences was shown to utilize glucose or ethanol for mixotrophic and heterotrophic growth. The highest cell density, 550 mg L− 1 dry weight after culture for 8 days, was obtained during mixotrophic culture with 30 mM glucose. The organic carbon sources had no effect on the net photosynthetic rate, but enhanced the respiratory rate. The addition of an organic carbon source led to an increase in the cell lipid content and a decrease in their eicosapentaenoic acid (EPA) content. The EPA yield was 21.9 mg L− 1 using photoautotrophic culture, and 23.4 mg L− 1 and 23.0 mg L− 1, respectively, in mixotrophic cultivation with glucose or ethanol as the carbon source. 相似文献
20.
The growth of Hansenula polymorpha and Kloeckera sp. 2201 with a mixture of glucose and methanol (38.8%/61.2%, w/w) and the regulation of the methanol dissimilating enzymes alcohol oxidase, catalase, formaldehyde dehydrogenase and formate dehydrogenase were studied in chemostat culture, as a function of the dilution rate. Both organisms utilized and assimilated glucose and methanol simultaneously up to dilution rates of 0.30 h-1 (H. polymorpha) and 0.26h-1, respectively (Kloeckera sp. 2201) which significantly exceeded max found for the two yeasts with methanol as the only source of carbon. At higher dilution rates methanol utilisation ceased and only glucose was assimilated. Over the whole range of mixed-substrate growth both carbon sources were assimilated with the same efficiency as during growth with glucose or methanol alone.In cultures of H. polymorpha, however, the growth yield for glucose was lowered by the unmetabolized methanol at high dilution rates. During growth on both carbon sources the repression of the synthesis of all catabolic methanol enzymes which is normally caused by glucose was overcome by the inductive effect of the simultaneously fed methanol. In both organisms the synthesis of alcohol oxidase was found to be regulated differently as compared to catalase, formaldehyde and formate dehydrogenase. Whereas increasing repression of the synthesis of alcohol oxidase was found with increasing dilution rates as indicated by gradually decreasing specific activities of this enzyme in cell-free extracts, the specific activities of this enzyme in cell-free extracts, the specific activities of catalase and the dehydrogenases increased with increasing growth rates until repression started. The results indicate similar patterns of the regulation of the synthesis of methanol dissimilating enzymes in different methylotrophic yeasts.Abbreviations and Terms C1
Methanol
- C6
glucose; D dilution rate (h-1)
-
D
c
critical dilution rate (h-1)
-
q
s
specific, rate of substrate consumption (g substrate [g cell dry weight]-1 h-1)
-
q
CO2 and q
O2
are the specific rates of carbon dioxide release and oxygen consumption (mmol [g cell dry weight]-1 h-1)
- RQ
respiration quotient (q
CO2
q
O2
1
)
-
s
0(C1) and s
0(C6)
are the concentrations of methanol and glucose in the inflowing medium (g l-1)
-
s
residual substrate concentration in the culture liquid (g l-1)
- Sp. A.
enzyme specific activity
- x
cell dry weight concentration (gl-1)
-
Y
X/C6
growth yield on glucose (g cell dry weight [g substrate]-1 相似文献