首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 31 毫秒
1.
In embryos of albino mice of ICR strain, collected between days 13 and 15, the epithelial lining within the future upper maxillary diastema was studied using frontal histological sections stained with hematoxylin-eosin and PAS methods. In embryos harvested on the 12th hr of day 13 (stage 13/12), a continuous epithelial rudiment of dentition was found in the anterior extension of the epithelial anlage of the first upper molar, up to the level of the lower anterior margin of the primary choana. In this stage the rudiment acquired, in the most anterior region of the future diastema, an arrangement typical for the dental lamina. In its dorsal extension there was found a distinct tooth anlage at the transitory stage lamina bud, which further (at stage 13/24) disintegrated into several segments. Starting with the day 14 (stage 14/12), the epithelial rudiment of dentition within the future upper diastema began to regress. From the stage 14/24 on, the anlage persisted only in its posterior terminal part where it merged with the epithelial lamina extending anteriorly from the anlage of the first upper molar. The existence of the dentition rudiment within the future mouse diastema constitutes the ontogenetic evidence that the diastema originates only secondarily--by regression. In some mutant strains of mice (tabby, crooked, sleek), the regression appears incomplete. The odontogenic potency of mouse diastema tissues should be considered when interpreting the results of in vitro experiments investigating the odontogenic inductive tissue interactions in mouse.  相似文献   

2.
The development and spatial arrangement of rugae palatinae was investigated using sagittal histological sections through the heads of 12- to 19-day mouse (ICR) embryos (appearance of vaginal plug = day 1) and scanning electron microscopy (SEM) visualization. Three distinct, consecutively occurring types of developing rugae were described: 1) rugal anlage (formed by thickened epithelium mostly burrowed in mesenchyme), 2) primitive ruga (transversally oriented band of thickened epithelium protruding to the oral cavity), and 3) definitive ruga (transversally oriented mesenchymal ridge protruding to the oral cavity, covered by epithelium). As the characteristic configuration and spatial patterns were found on each of days 13-19, rugae could be utilized as a natural positional marker (eg, in odontogenesis or palatogenesis studies). The foremost rugae start to differentiate between days 12 and 13. Between days 13 and 14 the number of rugae conspicuously increases in the anterior third of palatal shelf, and by palatal shelf horizontalization (day 15, a.m.) new rugae originate in the middle third. We presume that the origin of rugae is dependent upon inductive epithelial-mesenchymal interactions. The presence of developing rugae and the timing of their origin (occurrence of tissue interactions) in the middle versus the anterior third of the palatal shelf appears to be reflected in the mode of cellular arrangement, extracellular matrix (ECM) composition, and, probably, even in the elevation mechanism. Six stages of formation of the ruga were defined, and stage-dependent arrangements of epithelial and mesenchymal cells within the developing ruga were documented. A rearrangement of cells precedes the occurrence of the primitive ruga as well as its transformation to the definitive one. These events are discussed in relation to the hypothetical integrated function of cytoskeletal and ECM components. Regarding the developmental relation of rugae to maxillary dentition in the mouse, a comparison of particular stages of teeth and rugae development and analysis of their similarities and dissimilarities may extend the knowledge of general rules of morphogenesis and differentiation in oral biology.  相似文献   

3.
Epithelial duodenal anlage takes place at the end of the first month of the human embryogenesis in connection with appearance of the pancreatic epithelial germs. It has a form of a short arch with a spindle-like enlargement of the middle ductal part. Formation of the hepato-duodenal ligament and of the duodenal mesentery results in appearance of the given organ anlage with a three-layered wall in 5.5-6-week-old embryos. The anlage makes an arch, protruding to the right and forward with a spindle-like enlargement of the middle ductal part. It is tightly connected with the forming anlage of the pancreatic head, when the pancreatic epithelial anlages are fusing. An intensive growth of the pancreatic head is accompanied with emergence and enlargement of the superior, descending and inferior parts of the duodenum, that acquires a semicircular form in 8-8.5-week-old embryos. Its transitional stage is a curved semicircle having a form of a distended spiral coil; that reflects an uneven growth of the organ in the human embryogenesis.  相似文献   

4.
川金丝猴雌性生殖器官的观察   总被引:1,自引:0,他引:1  
对3只川金丝猴(Rhinopithecus roxellanae)的雌性生殖器官进行了观察。结果表明,卵巢通常呈椭圆形,两端稍尖,长1.0~1.8cm,宽0.4、0.6cm,厚0.2~0.4cm。有一个体呈一端大、一端小,中间缩细类似亚铃的形状。输卵管两侧均有系膜固定,输卵管上系膜仅0.1~0.2cm宽。子宫略呈长简形,子宫底正中沟不明显,子宫颈管较长,子宫轴与阴道轴在一直线上,子宫颈阴道部明显突出,前唇尤为明显,而阴道后穹比前穹深得多。阴道下半部前壁处皱襞密而高,形成三角形隆起,尖端向尿道外口:阴道前庭内前庭球明显,长约0.5cm,宽约0.2cm,位于尿道外口两侧的椭圆形窝内。  相似文献   

5.
V M Barabanov 《Ontogenez》1991,22(2):175-181
This is a review of the literature and author's own data on determination of various cell types of adenohypophysis during embryonic development. Recent studies using techniques of organ culture and immunohistochemistry have established the time of determination of glandular cells of adenohypophysis. It has been shown in rat embryos that the direction of differentiation of all major cell types of adenohypophysis is programmed late during the development of the epithelial anlage of this organ. Similar data as concerns somatotropic and prolactin cells have been obtained on chick embryos. Chick embryos possess regional type of determination of prolactin and somatotropin-containing cells in the anlage in correspondence with their location in definitive adenohypophysis.  相似文献   

6.
The purpose of this study was to document the extent of disruption in the pattern of palatal rugae caused by the presence of one copy of the First arch mutation. The palatal ruga pattern was found to be disrupted in 86% of 15- to 17-day mouse fetuses that were heterozygous for the First arch mutation in the ICR/Bc strain, compared with 9% in ICR/Bc fetuses of normal (+/+) genotype. This new observation in First arch heterozygotes, together with the previously reported dominant effects of the First arch mutation, particularly the bifurcation of the maxillary nerve (100% in both BALB/cGaBc and ICR/Bc strains), the disruption of maxillary vibrissa pattern (80% in ICR/Bc), and the hemifacial deficiency (38% in ICR/Bc), has led us to redefine the First arch mutation as a semidominant, Far. Like the other defects caused by Far, the rugal defects are in tissue derived from the embryonic maxillary prominence. The rugal defects observed in +/Far palates were always asymmetrical and most often involved fragmentation and misalignment of two or more of rugae 4-7. The relatively large degree of variation in ruga pattern observed in fetuses of normal genotype suggests that it is a less well canalized trait than the normal pattern of maxillary vibrissae which varies only in a few very specific and minor ways. The First arch mutation, which in heterozygotes disrupts pattern formation in both palatal rugae and maxillary vibrissae, can be used to study genetic control of pattern formation in mammalian embryos.  相似文献   

7.
Dentition development and budding morphogenesis   总被引:4,自引:0,他引:4  
The development of functional teeth in the mouse has been widely used as a model to study general mechanisms of organogenesis. Compared with other mammals, in which three incisors, one canine, four premolars, and three molars may occur even in each dental quadrant, the mouse functional dentition is strongly reduced. It comprises only one incisor separated from three molars by a toothless gap diastema at the location of the missing teeth. However, mouse embryos also develop transient vestigial dental primordia between the incisor and molar germs in both the upper and lower jaws. These rudimental structures regress, and epithelial apoptosis is involved in this process. The existence of the vestigial dental structures allowed a better assessment of the periodicity in the mouse dentition, which extends opportunities for the interpretation of molecular data on tooth development. We compared the dentition development with tentative models of budding morphogenesis in other epithelial appendages lungs and feathers. We suggested how developmental control by signaling molecules, including bone morphogenetic protein (Bmp), sonic hedgehog (Shh), and fibroblast growth factor (Fgf), can be similarly involved during budding morphogenesis of dentition and other epithelial appendages. We propose that epithelial apoptosis plays an important role in achieving specific features of dentition, whose development involves both budding and its more complex variant branching. The failure of segregation of the originating buds supports the participation of the concrescence of several tooth primordia in the evolutionary differentiation of mammalian teeth.  相似文献   

8.
V M Diewert  K Shiota 《Teratology》1990,41(6):663-677
Normal developmental events during human primary palate formation and alterations associated with cleft lip remain poorly defined. The purpose of this study was to analyze serially sectioned human embryos to identify morphological changes during normal palatal closure and alterations associated with failure of palatal formation. Normal and cleft embryos from the histological collection at the Congenital Anomaly Research Center at the University of Kyoto were studied and photographed for detailed evaluation. Seven serially sectioned cleft lip embryos of stages shortly after primary palate formation (Streeter-O'Rahilly stages 19, 20, and 22) with unilateral or bilateral clefts with varying degrees of clefting were studied. In the normal Kyoto embryos, initial nasal fin (epithelial seam) formation was observed between the medial nasal process and the lateral nasal and maxillary processes at stage 17. During stages 18 and 19, the nasal fin epithelium was replaced by an enlarging mesenchymal bridge, as the maxillary processes united with the medial nasal processes to form the primary palate. The most prominent features observed in the cleft embryos were a reduced thickness of mesenchymal bridging between the medial nasal and maxillary processes, with an excessive amount of epithelium at the junctions between these processes. With ingrowth of the maxillary processes, greater cell dispersion and apparent extracellular matrix accumulation were observed in the medial nasal region. During closure of the primary palate, terminal branches of the maxillary nerve crossed the mesenchymal bridge to the medial nasal region. The partial clefts had reduced maxillary ingrowth and smaller union areas with the medial nasal process. Detailed studies of experimental animal models are required to identify regional growth required for contact between the facial prominences, to clarify the mechanisms of mesenchymal ingrowth and epithelial displacement during palatal formation, and to identify local and/or general factors causing alterations that lead to primary palatal clefting.  相似文献   

9.
In 285 human embryos, prefetuses, fetuses and newborns by means of a complex of morphological methods, development of the epididymal canalicular system has been studied. The anlage of the epididymal canalicular system is stated to appear in embryos 13.0-17.0 mm long (the 6th week of development) and is presented as an accumulation of epithelial cells and primary germ cells between reducing glomeruli and mesonephric canaliculi. The canalization process of the cellular accumulations and their transformation into real canaliculi takes place during the first half of the prefetal period (the 8th week of development). Further growth and development of the epididymal canaliculi takes place in the craniocaudal direction and by the end of the prefetal period (the 12th week) the canaliculi of the head are already formed, they have a twisted course; within the limits of the body and tail they are yet poorly differentiated and their course is nearly straight. During the fetal period of ontogenesis, further differentiation of the epididymal parts occurs and structure of their canaliculi becomes more complex. They acquire a twisted course in all the parts of the organ; by the end of the fetal period the form and structure of the epididymal canaliculi resemble those of the definitive organ.  相似文献   

10.
Proteome analysis of developing mice diastema region   总被引:1,自引:0,他引:1  
Chae YM  Jin YJ  Kim HS  Gwon GJ  Sohn WJ  Kim SH  Kim MO  Lee S  Suh JY  Kim JY 《BMB reports》2012,45(6):337-341
Different from humans, who have a continuous dentition of teeth, mice have only three molars and one incisor separated by a toothless region called the diastema in the hemi mandibular arch. Although tooth buds form in the embryonic diastema, they regress and do not develop into teeth. In this study, we evaluated the proteins that modulate the diastema formation through comparative analysis with molar-forming tissue by liquid chromatography-tandem mass spectroscopy (LC-MS/MS) proteome analysis. From the comparative and semi-quantitative proteome analysis, we identified 147 up- and 173 down-regulated proteins in the diastema compared to the molar forming proteins. Based on this proteome analysis, we selected and evaluated two candidate proteins, EMERIN and RAB7A, as diastema tissue specific markers. This study provides the first list of proteins that were detected in the mouse embryonic diastema region, which will be useful to understand the mechanisms of tooth development.  相似文献   

11.
Summary The avian stomach is subdivided into two parts, the proventriculus and the gizzard. It has been shown that the gizzard epithelium can express embryonic chick pepsinogen (ECPg) antigen, a marker protein of the proventricular epithelium, as well as normal proventricular epithelium, under the appropriate experimental conditions. To study the possible mechanisms involved in the suppression of ECPg synthesis in the gizzard epithelium during normal development, we carried out heterotypic and heterochronic recombination experiments of the epithelium and mesenchyme of these two organ rudiments. When recombined and cultured with 6-day proventricular mesenchyme, gizzard epithelium of 3.5- to 12-day embryos expressed pepsinogen at all stages tested. However, the ratio of ECPg-positive cells to total epithelial cells in the gizzard epithelium decreased rapidly when epithelium older than 7 days was cultured with proventricular mesenchyme. In contrast to proventricular mesenchyme, 6-day gizzard mesenchyme did not allow ECPg expression in associated proventricular epithelium of 3.5- to 7-day embryos. These results indicate that gizzard epithelium does not express pepsinogen in normal development because of both a decrease in ability to express the enzyme in itself in the course of development and a repressive influence of gizzard mesenchyme.  相似文献   

12.
The ontogenetic developments of the pineal organ, parapineal organ, and retina were studied by the use of light and electron microscopy in embryos and fry of the teleost, Gasterosteus aculeatus, from 60 to 168 h after fertilization. Sixty to 66 h after fertilization, the primordium of the pineal complex is discernible in the diencephalic roofplate; the parapineal anlage is located rostral to the pineal anlage. Photoreceptor cells endowed with outer segments are present in the embryonic pineal organ already after 72 h, whereas outer segments of retinal photoreceptors could not be demonstrated before 144 h (hatching occurs between 120-144 h). Furthermore, neuropil formations with synaptic specializations are present in the rostral part of the pineal organ 108 h after fertilization. At 72 h, the embryonic parapineal parenchyma is already differentiated into parapinealocytes, which give rise to the parapineal tract, and glia-resembling elements. Although parapinealocytes carry cilia (9 X 2 + 0), only a single outer segment of the photoreceptor type could be demonstrated in the parapineal organ of one adult stickleback. Photoreceptors present in the pineal organ of unhatched embryos are hardly involved in visual functions, but may already at this early developmental stage serve as photoneuroendocrine transducers.  相似文献   

13.
In human embryos, prefetuses and fetuses from 6 to 40 weeks of development 82 preparations of the pancreas have been studied; 39 series of slices of the human embryos have been impregnated after Bielschowsky-Boeke. For the first time the nerves grow into the pancreas anlage in embryos of 16 mm long (6 weeks). The main sources supplying the pancreas with the nerves in the period studied are nervus vagus, celiac, hepatic, splenic, right adrenal, superior mesenteric and intermesenteric plexuses. Three stages in formation of the innervational apparatus of the pancreas are distinguished during the intrauterine development. The first stage is characterized by beginning of the nerve growing into the pancreas anlage. For the second stage an increased development of the intraorganic innervational apparatus and appearance of the all main sources of the organ's innervation are specific. The third stage is characterized with an increased differentiation of receptor zones in various parts of the pancreas. The development of the pancreatic innervational apparatus by the time of birth is not completed.  相似文献   

14.
We conducted a comprehensive meta-analysis of 12 studies to examine whether maxillary protraction face mask associated with rapid maxillary expansion (FM/RME) could be an effective treatment for Class III malocclusion and to evaluate the effect of timing on treatment response. Patients with a maxillary deficiency who were treated with FM with or without RME were compared with those who had an untreated Class III malocclusion. In both treatment groups, forward displacement of the maxilla and skeletal changes were found to be statistically significant. In addition, posterior rotation of the mandible and increased facial height were more evident in the FM group compared with the control group. However, no significant differences were observed between the early treatment groups and late treatment groups. The results indicated that both FM/RME and FM therapy produced favorable skeletal changes for correcting anterior crossbite, and the curative time was not affected by the presence of deciduous teeth, early mixed dentition or late mixed dentition in the patient.  相似文献   

15.
Epithelial appendages on palatal rugae develop during mouse palatogenesis through epithelial thickening and pattern formation. Recently, the patterned formation of nine rugae was observed together with the specific expression patterns of Shh in rodents. However, no crucial evidence was found for a direct association between Shh expression and the distinct structural formation of rugae. In order to reveal possible relationships, we investigated the morphological changes of rugae and expression patterns of Shh directly by in vitro organ culture at embryonic day 13 (E13) for 2 days. To compare and examine the diverse growing aspects of the palate and rugae, we carefully observed the detailed morphogenesis, with cell proliferation of the rugae occurring between E13 and E14.5. After 2 days of cultivation at E13, DiI micro-injections revealed that the middle part of the palate, adjacent to the upper molar-forming region, contributed to the formation of the subsequent structure of rugae by extensive cell rearrangement and proliferation within the epithelium in the preferred anteroposterior direction. The results also defined the intimate relationship between Shh expression and rugae formation.  相似文献   

16.
Summary The choroid plexus consists of the choroidal epithelium, a derivative of the neural tube, and the choroidal stroma, which originates from the embryonic head mesenchyme. This study deals with epithelio-mesenchymal interactions of these two components leading to the formation of the organ. Grafting experiments of the prospective components have been performed using the quail-chicken marker technique. Prospective choroidal epithelium of quail embryos, forced to interact with mesenchyme of the body wall of chicken embryos, gives rise to a choroid plexus showing normal morphogenesis and differentiation. The choroidal epithelium induces the differentiation of organtypical fenestrated capillaries, which are highly permeable to intravenously injected horseradish peroxidase. The choroidal epithelium of the grafts constitutes a blood-cerebrospinal fluid barrier. On top of the choroidal epithelium, there are epiplexus cells displaying a typical ultrastructure. The experimental results show that these cells do not originate from the transplanted neural epithelium. Prospective choroidal stroma of chicken embryos does not exert a choroid plexus-inducing influence upon a quail embryo's neural epithelium isolated from parts of the brain that normally do not develop a choroid plexus. The experiments show that the choroidal epithelial cells are determined at least three days before the first organ anlage is detectable.This work was supported by the Deutsche Forschungsgemeinschaft (grant Ch 44/7-1)  相似文献   

17.
Macrostomatan snakes, one of the most diverse extant clades of squamates, display an impressive arsenal of cranial features to consume a vast array of preys. In the absence of indisputable fossil representatives of this clade with well-preserved skulls, the mode and timing of these extraordinary morphological novelties remain obscure. Here, we report the discovery of Kataria anisodonta n. gen. n. sp., a macrostomatan snake recovered in the Early Palaeocene locality of Tiupampa, Bolivia. The holotype consists of a partial, minute skull that exhibits a combination of booid and caenophidian characters, being the presence of an anisodont dentition and diastema in the maxilla the most distinctive trait. Phylogenetic analysis places Kataria basal to the Caenophidia+Tropidophiidae, and represents along with bolyeriids a distinctive clade of derived macrostomatans. The discovery of Kataria highlights the morphological diversity in the maxilla among derived macrostomatans, demonstrating the relevance of maxillary transformations in the evolution of this clade. Kataria represents the oldest macrostomatan skull recovered, revealing that the diversification of macrostomatans was well under way in early Tertiary times. This record also reinforces the importance of Gondwanan territories in the history of snakes, not only in the origin of the entire group but also in the evolution of ingroup clades.  相似文献   

18.
It has been shown by autoradiography using 3H-thymidine that 11-day mouse embryos doubly homozygous for the autosomal recessive genes fidget (gene symbol fi) and ocular retardation (or), have three to five times fewer labelled nuclei in their retina anlages as do normal (genotype +/+ +/+) embryos singly homozygous for fidget (+/+ fifi) or ocular retardation (+/+ oror). In 11-day embryos of +/+ +/+, +/+ fifi and +/+ oror genotypes the labelled nuclei are localized mainly in the inner zone of the retina anlage. However, in double homozygotes the indices of labelled nuclei were not significantly different in the inner and outer zones of the retina anlage. The retina anlage of 12-day double homozygote, fifioror, has practically no nuclei synthesizing DNA. Consequently, the mutant genes fi and or which prolong the G1 period of the cell cycle in single homozygotes, act synergetically to stop DNA synthesis in the retina anlage cells of 12-day fifioror embryos.  相似文献   

19.
The external features of the developing embryos of the springtail, Tomocerus ishibashii, are described. The clypeolabral anlage arises as a single, unpaired swelling. The entognathy is completed by the ventral growth of the tergal anlagen of mandibular, maxillary, and labial segments. These anlagen also form the posterior part of the cranium. The palpi of maxilla and labium are homologous with the telopodites, and proximal parts of these head appendages are homologous with the coxopodites. The sternal element of the labial segment does not participate in the postmentum formation. The anlagen of abdominal appendages appear in the first to the fourth abdominal segments. The first, third, and fourth appendage anlagen form the ventral tube, tenaculum, and furcula, respectively. The fused proximal parts of the first, third, and fourth appendage anlagen are homologous with the coxopodites, and the distal parts which do not fuse are homologous with the telopodites. The anlagen of the second abdominal appendages become flattened and spread over the ventral side of this segment. The ventral structures of the first four abdominal segments are appendicular in origin.  相似文献   

20.
Scanning electron microscopy of exposed surfaces of the porcine placenta   总被引:1,自引:0,他引:1  
V Dantzer 《Acta anatomica》1984,118(2):96-106
The three-dimensional development of the separated parts of the porcine placenta from 9 Danish Landrace sows at gestational stages from 20 to 100 days was studied. After cautious separation of the allantochorion and the endometrium in a 1 mM buffered ethylenediaminetetraacetic acid solution, the separated parts were processed for scanning electron microscopy by routine methods. The macroscopic enlargement resulted from primary and secondary circular folds or plicae, which were permanent on the maternal side, whereas they were mainly non-permanent on the fetal side. The areolar placenta and interareolar placenta with formation of permanent microscopic folding on both sides were described. The observations of the separated parts yielded new information on the development of surface enlargement during gestation and revealed a hitherto unknown regular architecture of the endometrium by the formation of parallel primary ridges or rugae with secondary ridges or rugae at their sides subdividing the maternal troughs or fossae. This configuration on the maternal side explains the transformation of the regular chorionic ridges from the 63-day stage into bulbous protrusions at the 100-day stage. Based on these observations the precise terminology used above was proposed.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号