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1.
Marta Vila Neus Marí-Mena Shen-Horn Yen Carlos Lopez-Vaamonde 《Conservation Genetics》2010,11(3):1151-1154
Ten polymorphic microsatellite loci were developed for Graellsia isabelae. Polymorphism was assessed for 20 individuals from a Spanish population (Els-Ports-de-Beseit, Catalonia) and 39 more individuals
from one population in the French Alps and six other Spanish localities. Overall, the number of alleles per locus ranged from
5 to 24. Els-Ports-de-Beseit showed an average number of alleles per locus of 9.80 (SD = 4.32), observed heterozygosity was
0.71 (SD = 0.226), and expected heterozygosity was 0.788 (SD = 0.146). Genotypic frequencies conformed to Hardy–Weinberg equilibrium
at the Catalonian population, and no evidence for linkage disequilibrium was observed. Multilocus genotypes resulting from
this set of markers will be useful to determine genetic diversity and differentiation within and among populations of this
highly protected moth. Several loci amplified and resulted polymorphic in two related species: two loci in Actias neidhoeferi, and three loci in A. luna. 相似文献
2.
In order to study hybridisation, taxonomic boundaries and phylogeography in the genus Pseudopanax (Araliaceae), we developed seven novel microsatellite loci from enriched genomic libraries of P. lessonii and P. crassifolius. These loci were characterised in 16 individuals from a single population of P. crassifolius, and displayed 2–11 alleles per locus. Observed heterozygosities ranged from 0.063 to 0.688. Most loci were polymorphic in
the closely related Pseudopanax species, and several loci amplified widely across the Araliaceae. 相似文献
3.
Selvaraju Kanagarajan Saraladevi Muthusamy Emily Chin-Fun Chen Hsin-Sheng Tsay 《Conservation Genetics》2009,10(5):1487-1490
We developed and characterized 8 polymorphic microsatellite loci or simple sequence repeats from the expressed sequence tags
database of the medicinal fungus, Antrodia cinnamomea. Variability was assessed in a panel of 23 strains collected from various regions of Taiwan. The number of alleles per polymorphic
locus ranged from two to seven loci with an average of 4.375 alleles per locus. The observed and expected heterozygosity values
ranged from 0.130 to 0.783 and from 0.122 to 0.809, respectively. No significant Hardy–Weinberg equilibrium was detected (P < 0.01) at all loci. The EST-SSR markers developed in the present study can be used for strain identification and population
genetic studies in A. cinnamomea. 相似文献
4.
We isolated 20 anonymous nuclear loci (8556 bp in total) from the Taiwan Hwamei (Garrulax taewanus), an endemic songbird of Taiwan. A panel of nine to 15 individuals with unknown relationship was used to characterize polymorphism of these loci. We identified 46 single nucleotide polymorphic sites (SNPs) in 15 polymorphic loci. Frequency of SNPs was one per every 186 bp in average. Nucleotide diversity, θ, ranged from 0.00054 to 0.00371 per locus. We also tested cross‐species applicability of these loci on 17 species from eight different passerine families. All 20 loci could be successfully amplified (ranged from one to 16 species, mean = 7.9 species). 相似文献
5.
Soo Hyung Eo Woo-Shin Lee Travis C. Glenn Kenneth L. Jones Eun-Jae Lee Yong-Su Park John P. Carroll Shin-Jae Rhim 《Conservation Genetics》2009,10(6):1923-1925
We isolated and characterized eight polymorphic microsatellite DNA markers from the Korean field mouse, Apodemus peninsulae. The primers developed in this study yielded an average polymorphic information content of 0.78 (range 0.44–0.90), with an
average of 10.9 alleles per locus (range 5–16). Observed and expected heterozygosities ranged from 0.46 to 1.00 and from 0.49
to 0.93, respectively. These polymorphic loci may provide useful tools for understanding the species’ genetic structure and
ecology. 相似文献
6.
Aliya El Nagar Matthew McHugh Toby Rapp David W. Sims Martin J. Genner 《Conservation Genetics》2010,11(3):1203-1206
Simple sequence repeat markers (SSRs, microsatellites) were characterised for skates (Elasmobranchii: Rajidae) from published
expressed sequence tags (ESTs) of Leucoraja erinacea. These were tested in four European species (Raja clavata, Raja montagui, Dipturus batis, and Leucoraja naevus). Thirteen of the fourteen amplified loci were polymorphic in at least one species. Polymorphic loci possessed on average
4.5–5.9 alleles per species, and expected heterozygosity ranged from 0.05 to 0.88. Possible null alleles were detected at
three loci, while one locus showed significant deviation from Hardy–Weinberg Equilibrium proportions. Three locus-pairs exhibited
significant linkage disequilibrium in one or more species. This marker set will be valuable for population genetic analyses
of the focal taxa, and may prove useful for studies of other skate species. 相似文献
7.
Guidong Miao Changwei Shao Hongyu Ma Xiaolin Liao Songlin Chen 《Conservation Genetics》2009,10(3):631-633
Starry flounder (Platichthys stellatus) is a rare fish species in China. Here, we reported 12 polymorphic microsatellite loci isolated from a dinucleotide-enriched
genomic library of starry flounder (P. stellatus). The number of alleles, observed and expected heterozygosity per locus in 30 individuals ranged from two to six, from 0.2500
to 1.0000 and from 0.4512 to 0.7667, respectively. One locus significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species amplification
of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite loci would be
useful for investigating genetic population structure and construction of genetic linkage map in P. stellatus.
Guidong Miao and Changwei Shao have contributed equally. 相似文献
8.
Fourteen polymorphic microsatellite markers were isolated from the Hong Kong oyster, Crassostrea hongkongensis, with a partial genomic library enriched for tandem repeat sequences of (CA)12, (GA)12, (ATG)6 and (TAGA)4. Polymorphism of these loci was assessed in a sample of 48 wild unrelated individuals. The average allelic number of these
polymorphic loci was 6.36 per locus, with a range of 4–16. The observed and expected heterozygosity varied from 0.208 to 0.729
(averaging 0.502) and from 0.193 to 0.789 (averaging 0.615), respectively. After Bonferroni correction (P > 0.0036), 11 of the 14 loci accorded with Hardy–Weinberg equilibrium, and the rest three were detected significant departure
from HWE. Additionally, two loci (Ch103 and Ch104) showed significant linkage disequilibrium (P < 0.01). This is the first set of microsatellite loci developed in this species and would be useful for studies of population
genetics, stock management and other relevant research in C. hongkongensis. 相似文献
9.
Microsatellite loci were identified for three closely related penaeid species, Farfantepenaeus subtilis, F. paulensis and F. sp., from genomic libraries enriched for CA repeats. Seven out of nine highly polymorphic loci detected were amplified across all three species. Between four and 64 alleles were recorded per locus (average = 36). The average observed heterozygosity ranged from 0.094 to 0.897 (mean = 0.613), while the expected heterozygosity ranged from 0.091 to 0.985 (mean = 0.822). 相似文献
10.
Caitríona E. McInerney A. Louise Allcock Mark P. Johnson Paulo A. Prodöhl 《Conservation Genetics》2009,10(5):1417-1420
Eight polymorphic microsatellite loci are described for Littorina littorea (Linnaeus, 1758). Data on allelic variation in Irish and Celtic Sea samples are reported. The average number of alleles per
locus was 11 (range 4–29), and observed and expected heterozygosities ranged from 6.9 to 84.3% and from 9.4 to 95.2%, respectively.
Loci did not deviate from Hardy–Weinberg equilibrium and no linkage disequilibrium between loci pairs was detected. Microsatellites
were not highly conserved in the congeners, L. fabalis, L. saxatilis, L. compressa and L. obtusata as evidenced by a low rate of cross-amplification. These microsatellites should prove useful in population genetic studies. 相似文献
11.
We report ten microsatellite loci in the Emei moustache toads, Leptobrachium boringii. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Twenty-four individuals from one
breeding site were examined and ten loci were polymorphic. The number of alleles per locus ranged from 3–9 with an average
of 6.3/locus. The expected heterozygosity and observed heterozygosity ranged from 0.3874 to 0.8432, and from 0.4583 to 0.9167,
respectively. Cross-species amplification was tested in a closely related species L. leishanensis. These markers will be useful in future studies on characterizing the mating system of the species. 相似文献
12.
Tzen-Yuh Chiang Hung-Du Lin Tin-Yam Chan Cheng-Yu Hung Feng-Jiau Lin 《Conservation Genetics》2008,9(6):1653-1655
Populations of mudshrimp, Austinogebia edulis, in the intertidal mud flat of western Taiwan have severely declined due to habitat destruction and overfishing in the past
decades. Nine polymorphic microsatellite loci were isolated for this ecologically threatened species by using a polymerase
chain reaction-based procedure. The number of alleles per locus ranged from five to 14 in 20 specimens from Central West Taiwan.
Observed and expected heterozygosities ranged from 0 to 0.2500 (averaged at 0.0944) and 0.7333 to 0.9385 (averaged at 0.8524),
respectively. There were significant departures from Hardy–Weinberg expectations. Significant LD was discovered in most loci.
These primers may provide a tool for understanding population structure in A. edulis.
Tzen-Yuh Chiang and Hung-Du Lin contributed to the study equally. 相似文献
13.
Small yellow croaker (Larimichthys polyactis) is an important economic species of marine fishery. We developed and evaluated simple sequence repeat (SSR) markers from
expressed sequence tags (ESTs) of Pseudosciaena crocea, Paralichthys olivaceus and Psetta maxima. Characteristics of nine EST–SSR loci were investigated using 46 L. polyactis individuals. The number of alleles per locus ranged from 2 to 6. The observed heterozygosity ranged from 0.0652 to 0.7391,
while the expected heterozygosity ranged from 0.0638 to 0.7754. Seven loci departed from Hardy–Weinberg equilibrium (P < 0.01) significantly. These loci and markers will be useful for population genetics and systemic evolution among species
of small yellow croaker. 相似文献
14.
Claudete F. Ruas Thiago J. Nakayama M. Á. Ortiz Mayra A. Kuroki Tod F. Stuessy Karin Tremetsberger Eduardo A. Ruas Melissa de Oliveira Santos Salvador Talavera Paulo M. Ruas 《Conservation Genetics》2009,10(5):1413-1416
We report the isolation and characterization of eight microsatellite loci for Hypochaeris salzmanniana, an endangered species endemic to the southwestern coast of Spain and the Atlantic coast of Morocco. A total of 32 alleles
were detected across a sample of 45 individuals, with an average number of 4.0 alleles per locus. The average polymorphic
information content (PIC) was 0.533 and the observed (H
O) and expected (H
E) heterozygosity values varied from 0.022 to 0.978 and from 0.434 to 0.759, respectively. Five loci exhibited significant
deviation from Hardy–Weinberg equilibrium (P ≤ 0.001) and three pairs of loci showed significant linkage disequilibrium (P ≤ 0.01). The eight loci were tested for transferability in three others species (H. arachnoidea, H. glabra, and H. radicata) belonging to the same section of H. salzmanniana. With the exception of locus Hsalz7, all loci successfully amplified in the three species. These preliminary data confirm
the usefulness of microsatellite markers for assessing the ecology and genetic structure of H. salzmanniana and to understand the evolution of species within the section Hypochaeris. 相似文献
15.
Yuan Huang Yong Li Xiao Hu Xue-Jun Ge Chang-Qin Zhang Chun-Lin Long 《Conservation Genetics》2009,10(3):515-517
A total of twelve polymorphic microsatellite loci were developed from polyploid endangered species, Omphalogramma vincaeflora (Primulaceae). These loci were screened for variability among 45 individuals from three populations in China. The primers
amplified loci with allele number ranging from 3 to 9, with an average of 4.25 per locus. Polymorphism information content
ranged from 0.23 to 0.86. Nei’s genetic diversity ranged from 0.34 to 0.86. These primers provide an opportunity to use polymorphic
DNA markers to study the population genetic structure and its breeding system in this species. 相似文献
16.
Eleven microsatellite loci have been developed from Fagus longipetiolata and the loci were characterized for 21 individuals. All eleven loci were polymorphic, with 2–8 alleles and an average of
4.8 per locus. The observed (H
O) and expected (H
E) heterozygosities were 0.053–0.714 and 0.355–0.856, respectively. There was significant deviation from Hardy–Weinberg equilibrium
at two loci. No locus pair had significant linkage disequilibrium. Cross-species amplifications of the markers were also tested
in three other congeneric species. 相似文献
17.
Carol K. L. Yeung Yu-Cheng Hsu Cheng-Te Yao Shou-Hsien Li 《Conservation Genetics》2009,10(4):1081-1084
We isolated 23 polymorphic microsatellite loci of tetranucleotide, dinucleotide or compound repeat motif in the black-faced
spoonbill (Platalea minor). In a panel of up to 20 individuals, number of alleles per locus ranged from two to 19 (mean = 8) and heterozygosity from
0.278 to 0.950 (mean = 0.606). Allele frequencies of five loci deviated from that expected under Hardy–Weinberg Equilibrium,
and one of them was likely Z-linked. Excluding these loci, the remaining 18 loci should provide a sufficient set of molecular
markers for use in ecological and conservation genetic investigation of this globally endangered wader species, and cross-species
amplification test suggests that they are potentially useful in other Ciconiiformes waterbirds. 相似文献
18.
Yong-Sheng Tian Gui-Dong Miao Chang-Wei Shao Xiao-Lin Liao Song-Lin Chen 《Conservation Genetics》2009,10(4):1041-1043
Stone flounder (Kareius bicoloratus) is a rare fish species in China. Here, we reported 11 polymorphic microsatellite loci isolated from a repeat-enriched genomic
library of stone flounder. The number of alleles, observed and expected heterozygosity per locus in 32 individuals ranged
from 3 to 6, from 0.1613 to 0.8667 and from 0.1549 to 0.7932, respectively. Two loci significantly deviated from Hardy–Weinberg
equilibrium after Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species
amplification of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite
loci would be useful for investigating genetic population structure and construction of genetic linkage map in Kareius bicoloratus.
Yong-Sheng Tian and Gui-Dong Miao are contributed equally. 相似文献
19.
Xin Zhang Wan-Hui Ye Hong-Lin Cao Zheng-Feng Wang Hao Shen Ju-Yu Lian 《Conservation Genetics》2009,10(6):1833-1835
We developed 11 polymorphic microsatellite primers from Canarium album, which is a famous Chinese fruit tree with diversified economy values. The observed and expected heterozygosities (H
o and H
e) ranged from 0.133 to 0.833 and 0.128 to 0.810, with averages of 0.607 and 0.629, respectively. Four loci were deviated from
Hardy–Weinberg equilibrium after Bonferroni correction (P < 0.01), but no linkage disequilibrium was detected among any pair of loci after Bonferroni correction (P < 0.05). Cross-species amplification in another important cultured species Canarium pimela showed that 10 of 11 primers were polymorphic, with number of alleles per locus ranging from 2 to 5. The primers will be
useful to explore further studies about introgressive hybridization and conversation of wild genetic resources of these two
species. 相似文献
20.
Ten polymorphic loci were isolated and characterized from the milkweed, Asclepias syriaca L., of North America. These loci successfully cross-amplified in A. exaltata L. Polymorphism ranged from two to 16 alleles per locus per species in 68 individuals of A. syriaca and 56 individuals of A. exaltata. Expected heterozygosities ranged from 0.017 to 0.851 and significant deviations from Hardy–Weinberg equilibrium were found
for two and three loci in A. syriaca and A. exaltata, respectively. No linkage disequilibrium was detected. These markers should prove useful for assessing population genetic
structure and interspecific gene flow in these and other species of Asclepias. 相似文献