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1.
MELANIE PEREZ ROGER BOUR JOSIE LAMBOURDIERE SARAH SAMADI MARIE‐CATHERINE BOISSELIER 《Molecular ecology resources》2006,6(4):1096-1098
The taxonomic status of the Greek Testudo species, marginated tortoise (Testudo marginata Schoepff, 1793) and dwarf marginated tortoise (Testudo weissingeri Bour, 1995), is controversed. In order to study the gene flow among these Greek Testudo species, eight polymorphic microsatellite markers were characterized and screened for 32 individuals. The number of alleles observed per locus ranged from two to 19. Observed heterozygosities varied from 0.235 to 0.830. These microsatellite loci were also successfully tested on seven closely related species of Testudinidae. This set of microsatellites offers an efficient tool to investigate genetic differentiation among testudinid species. 相似文献
2.
S. Siju K. Dhanya S. Syamkumar B. Sasikumar T. E. Sheeja A. I. Bhat V. A. Parthasarathy 《Molecular biotechnology》2010,44(2):140-147
Expressed sequence tags (ESTs) from turmeric (Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total
of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average
density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite
repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST–SSRs were developed and used for evaluating
20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated
in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this
study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus. 相似文献
3.
Fraser LG McNeilage MA Tsang GK Harvey CF De Silva HN 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,112(1):149-157
Microsatellite marker transfer across species in the dioecious genus Actinidia (kiwifruit) could offer an efficient and time-effective technique for use during trait transfer for vine and fruit improvement in breeding programmes. We evaluated the cross-species amplification of 20 EST-derived microsatellite markers that were fully informative in an Actinidia chinensis mapping family. We tested all 20 markers on 120 genotypes belonging to 21 species, 5 with varieties and/or chromosome races. These 26 taxa included 16 diploids, 7 tetraploids, 2 hexaploids and 1 octaploid, and represented all four taxonomic sections in the genus. All 20 markers showed some level of cross-species amplification. The most successful marker amplified in all genotypes from all species from all sections of the genus, the least successful amplified fragments only in A. chinensis and A. deliciosa. One species, A. glaucophylla, failed to amplify with all but 2 markers. PIC (Polymorphism information content) values were high, with 14 of 17 markers recording values of 0.90 and above. Sequence data demonstrated the presence of the microsatellite in all the amplified products. Sequence homology was less 5′ of the microsatellite and increased toward the start codon of the translated region of the EST from which the marker was derived. The data confirm that EST-derived microsatellite markers from Actinidia species show cross-species amplification with high levels of polymorphism which could make them useful markers in breeding programmes. 相似文献
4.
M. DEMURA M. KAWACHI M. KUNUGI T. NISHIZAWA F. KASAI M. M. WATANABE 《Molecular ecology resources》2007,7(2):315-317
We have developed 11 microsatellite markers that are specific to Chattonella antiqua, C. marina, and C. ovata, the red tide‐forming harmful phytoplanktons. The 11 loci were amplified in the three species. The number of alleles per locus ranged from 5 to 16. The three species shared most microsatellite regions, although the genetic differences in specific loci were detected among them. These markers of the Chattonella species will be beneficial for biogeographical, detailed taxonomic, studies. 相似文献
5.
Qin Jiao Schmidt Bjorn Victor Cheng Fei Xie Songguang 《Zeitschrift fur angewandte Ichthyologie》2021,37(2):314-317
Genetic patterns observed among populations by neutral molecular markers is a major method used to reconstruct the invasion history of invasive species. The Shimofuri goby (Tridentiger bifasciatus) has invaded several lakes along the East Route of the South-to-North Water Transfer Project, China (ENST). However, neutral molecular markers for this goby are not currently available. In this study, fourteen novel polymorphic microsatellite loci were developed through next generation sequencing technology. These markers were also tested in an invasive population (Nansi Lake, a storage lake of ENST) consisting of 54 individuals. The average number of alleles per locus was 7.6 and ranged from 4 to 13. The average Ho was 0.587 and ranged from 0.404 to 0.852, and the average He was 0.733 and ranged from 0.564 to 0.851. The average PIC per locus was 0.689 and ranged from 0.521 to 0.824. Four markers (TB4, TB65, TB66 and TB26) were found to deviate from HWE after correction following the False Discovery Rate method. Null alleles were found in four loci (TB11, TB65, TB66 and TB26). These highly polymorphic microsatellite markers are useful for further study to reconstruct invasion history of Shimofuri goby. 相似文献
6.
Cupressus chenggiana S. Y. Hu (Cupressaceae) is an endemic and endangered conifer species in southwest China. In order to study the population
genetics and design the effective conservation methods, we aimed to develop microsatellite primers for this species in the
present study. We developed eight new microsatellite loci for this species through biotin capture method. Polymorphism of
each locus was further assessed in 18 individuals from three geographically distant populations. The number of alleles per
locus ranged from 6 to 11 with an average of 8.13. The observed and expected heterozygosities ranged from 0.219 to 0.296 and
from 0.374 to 0.470, with averages of 0.254 and 0.417, respectively. We further found that three of nine microsatellite loci
developed previously for another congeneric species showed polymorphic banding patters. We performed primer-crossing tests
of these loci in the other two congeneric species which are closely related to C. chenggiana (C. gigantea and C. duclouxiana). These microsatellite markers would be effective for analyzing genetic diversity and population genetic structure of this
species and its morphological differentiation with the close relatives. 相似文献
7.
Jing Meng Dezhu Li Tingshuang Yi Junbo Yang Xingfeng Zhao 《Conservation Genetics》2009,10(6):1973-1976
Eighteen microsatellite markers were developed from Rosa
odorata var. gigantea (Rosaceae), including 11 new microsatellite markers and 7 modified microsatellite loci having been developed from other Rosa species. About 27 wild individuals from 3 populations were used to screen polymorphism of these 18 microsatellite makers.
The average allele number of these markers was 3.9 per locus, ranging from 2 to 9. The expected and observed heterozygosities
varied from 0.2711 to 0.8043 and from 0.0370 to 0.5556, respectively. Cross-species amplification in other eight Rosa species showed their potential use for congeneric species. These microsatellite primers will be used for population genetics
studies, constructing genetic linkage maps or locating quantitative trait locus (QTL) of R. odorata var. gigantea and related species. 相似文献
8.
E. S. L. Santos C. B. M. Cerqueira-Silva G. M. Mori D. Ahnert R. X. Corrêa A. P. Souza 《Biologia Plantarum》2012,56(4):789-792
Seventeen polymorphic microsatellite markers were isolated from enriched genomic libraries for Theobroma cacao, providing additional tools for studying the genetic diversity and map saturation of this species. These markers were characterized in 32 accessions of the T. cacao germplasm collection from the Centro de Pesquisas do Cacau. The number of alleles at each locus varied from 2 to 8, with an average of 4.41 alleles per locus. The polymorphism information content varied from 0.060 to 0.695, with an average of 0.333. The markers characterized in this study will be employed in map saturation studies and diversity assessments of cacao genotypes. 相似文献
9.
A total of 26 simple sequence repeats were identified using a random amplified polymorphic DNA (RAPD) based technique in the Southeast Asian river catfish Mystus nemurus. We report on the characterization of five polymorphic microsatellite loci in M. nemurus. The average number of alleles per locus was 3.2. These are the first microsatellite loci that have been developed for this species. These markers should prove useful as tools for managing the brood stocks and for future aquacultural development of this species. 相似文献
10.
Chinese alligator (Alligator sinensis) is a critically endangered species endemic to China. In this study, we developed 11 novel microsatellite loci for this rare
species and applied them to examine genetic variation of indigenous alligators from Changxing Nature Reserve and America-born
Chinese alligators. The 11 polymorphic microsatellites presented a total of 31 alleles among 57 individuals scored, yielding
an average of 2.82 alleles per locus. One allele was unique to the American population but four private alleles were detected
in the Changxing population. The average expected and observed heterozygosities were 0.400 and 0.482 for the Changxing alligators
and 0.520 and 0.621 for the America-born individuals, respectively. These microsatellite markers would be useful tools in
the genetic examination of this endangered species. 相似文献
11.
Incarvillea mairei (H. Léveillé) Grierson (Bignoniaceae) is an endemic species to the Himalaya-Hengduan Mountains region. Here, we developed
13 microsatellite markers from I. mairei using a modified biotin-streptavidin capture method. Number of alleles per locus (NA) ranged from 3 to 7 with an average
of 4.615. The observed (H
O) and expected (H
E) heterozygosities were from 0.050 to 0.800 and from 0.249 to 0.815, respectively. Additionally, among the 13 identified microsatellite
markers, 12 of them were successfully amplified in other three congeneric species, and most of them showed polymorphic. Obtained
evidences suggest that these markers provide a useful tool for further study of the population genetic structure and the breeding
system in this species or/and infra-generic species. 相似文献
12.
13.
Aurélie Johanet Damien Picard Trenton W. J. Garner Deborah A. Dawson Ramiro Morales-Hojas Robert Jehle Didier Peltier Christophe Lemaire 《Conservation Genetics》2009,10(6):1903-1906
We have developed eight di- and tetranucleotide Lissotriton microsatellite markers. Eight loci were polymorphic in the palmate newt Lissotriton helveticus and six were polymorphic in the smooth newt L. vulgaris. Polymorphism detected in 33 and 37 individuals per species ranged from 3 to 15 alleles. These markers are suitable for the
investigation of population structure, genetic variation and taxonomic identification in the two focal species, and may also
be of use in other Lissotriton–Triturus species. 相似文献
14.
BRUNO STUDER FRANCO WIDMER JÜRG ENKERLI ROLAND K
LLIKER 《Molecular ecology resources》2006,6(4):1108-1110
Twelve microsatellite markers were isolated from Lolium multiflorum. Allelic variability and cross‐species amplification were assessed on 16 individuals of each of the three grassland species L. multiflorum, Lolium perenne and Festuca pratensis. Cross‐species amplification success was 100% for L. perenne and 83% for F. pratensis. The number of alleles detected ranged from one to 14 with an average of 3.4. While three microsatellite loci were polymorphic in all three species, one marker produced species‐specific alleles in all three species. These microsatellite markers provide a valuable tool for population genetic studies within and among species of the Festuca–Lolium complex. 相似文献
15.
We report the isolation and characterization of seven microsatellite loci from the Caribbean reef‐building coral, Montastraea annularis. All loci are polymorphic with allele numbers ranging from five to 31 and observed heterozygosities from 0.17 to 0.89. These loci can be used in assessing gene flow patterns and diversity of this stony coral species both for local coral reef management purposes as well as for elucidating population connectivity within the greater Caribbean basin. These markers should also be applicable to other species of Montastraea and for resolving taxonomic relationships within the M. annularis species complex. 相似文献
16.
The golden pompano (Trachinotus blochii) is a marine fish species in tropical regions. No information about genetic variation and population structure of wild populations
is available. A first set of 12 polymorphic microsatellites isolated from this species were characterized. The number of alleles
ranged from 5 to 14 with an average of 8.0 alleles per locus. All 12 markers conformed to HWE and were in linkage equilibrium.
These 12 microsatellite markers supply the first set of co-dominant DNA markers for studying population genetics of the species
T. blochii. 相似文献
17.
Sirin Firidin Rafet Cagri Ozturk Melike Alemdag Oguzhan Eroglu Yahya Terzi Ilyas Kutlu Zehra Duygu Duzgunes Eyup Cakmak Ilhan Aydin 《Journal of fish biology》2020,97(4):1154-1164
Turbot, Scophthalmus maximus, is a commercially important demersal flatfish species distributed throughout the Black Sea. Several studies performed locally with a limited number of specimens using both mitochondrial DNA (mtDNA) and microsatellite markers evidenced notable genetic variation among populations. However, comprehensive population genetic studies are required to help management of the species in the Black Sea. In the present study eight microsatellite loci were used to resolve the population structure of 414 turbot samples collected from 12 sites across the Black Sea. Moreover, two mtDNA genes, COI and Cyt-b, were used for taxonomic identification. Microsatellite markers of Smax-04 and B12-I GT14 were excluded from analysis due to scoring issues. Data analysis was performed with the remaining six loci. Loci were highly polymorphic (average of 17.8 alleles per locus), indicating high genetic variability. Locus 3/20CA17, with high null allele frequency (>30%), significantly deviated from HW equilibrium. Pairwise comparison of the FST index showed significant differences between most of the surveyed sampling sites (P < 0.01). Cluster analysis evidenced the presence of three genetic groups among sampling sites. Significant genetic differentiation between Northern (Sea of Azov and Crimea) and Southern (Turkish Black Sea Coast) Black Sea sampling sites were detected. The Mantel test supported an isolation by distance model of population structure. These findings are vital for long-term sustainable management of the species and development of conservation programs. Moreover, generated mtDNA sequences would be useful for the establishment of a database for S. maximus. 相似文献
18.
Dahai Gao Qian Wang Yuxia Wu Haiyan Xu Qiushi Yu Jianquan Liu 《Conservation Genetics》2008,9(4):953-955
Thellungiella
salsuginea (Brassiaceae) is a typical halophyte which can tolerate extreme cold, drought, and salinity. In order to understand the adaptive
evolution of this species in the arid habitats, it is important to know its genetic structure. In this study, 17 polymorphic
microsatellite loci were isolated and characterized from an enrichment genomic library of this species. We further assessed
the polymorphisms of each locus in 18 individuals from nine geographically distant populations. The number of alleles per
locus ranged from six to fourteen. The observed and expected heterozygosity ranged from 0.17 to 0.28 and 0.32 to 0.45, respectively.
These markers have been crossly checked in another congeneric species, T. halophila. These microsatellite markers will be useful for investigating population genetics and adaptive evolution of this species
and morphological divergence between and it and the closely related species. 相似文献
19.
Chinese alligator (Alligator sinensis) is a critically endangered species endemic to China. In this study, the extent of genetic variation in the captive alligators
of the Changxing Reserve Center was investigated using microsatellite markers derived from American alligators. Out of 22
loci employed, 21 were successfully amplified in the Chinese alligator. Sequence analysis showed loci in American alligators
had a bigger average size than that of the Chinese alligators and the longest allele of an individual locus almost always
existed in the species with longer stretch of repeat units. Eight of the 22 loci were found to be polymorphic with a total
of 26 alleles present among 32 animals scored, yielding an average of 3.25 alleles per polymorphic locus. The expected heterozygosity
(H
E) ranged at a moderate level from 0.4385 to 0.7163 in this population. Compared to that in the American alligators, a lower
level of microsatellite diversity existed in the Changxing population as revealed by about 46% fewer alleles per locus and
smaller H
E at the homologous loci. The average exclusion power and the ability to detect shared genotypes and multiple paternity were
evaluated for those markers. Results suggested that when the polymorphic loci were combined, they could be sensitive markers
in genetic diversity study and relatedness inference within the Chinese alligator populations. The level of genetic diversity
present in the current Changxing population indicated an important resource to complement reintroductions based on the individuals
from the other population. In addition, the microsatellite markers and their associated diversity characterized in this population
could be utilized to further investigate the genetic status of this species. 相似文献
20.
Over the past few decades, use of molecular markers for species delimitation has drastically increased. Schoenoplectiella Lye has been recognized as a taxonomically difficult genus because of its morphological simplicity and frequent interspecific
hybridization. The main objective of this study was to clarify the taxonomic identities of eight Schoenoplectiella species by use of molecular markers. We also evaluated the genetic relationships among S. × trapezoidea, known as a natural hybrid, and its close relatives. We used six microsatellite markers for 44 individuals from 31 natural
populations of eight Schoenoplectiella species in South Korea. Six microsatellite marker combinations generated 59 amplification-detectable bands, of which 33 were
specifically detected in one or more individuals of each species. Cluster analysis revealed that the grouping was consistent
with the taxonomically recognized species. Our results do not support the hybrid origin of S. × trapezoidea. Rather, they suggest that this species is more closely related to S. hotarui. The informative microsatellite markers enabled us to clarify the distinctions among Schoenoplectiella species from South Korea and to identify the genetic relationships among these species. The molecular signatures found suitable
for accurate identification of Schoenoplectiella species can be reliably used for studies of the phylogeny and evolution of this genus. 相似文献