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1.
The equilibrium uptake of hydrophilic solutes, D-glucose and L-carnitine, by large unilamellar phospholipid vesicles composed of egg lecithin (PC), phosphatidic acid (PA), and various concentrations of cholesterol (Chol) has been measured. Calculation of the encapsulated volume of PC-PA and PC-PA-Chol vesicles, based on electron-microscopy data, agreed with the values directly measured by fluorescence techniques. Likewise, vesicle surface areas determined directly and from electron microscopy were in good agreement. Equilibrium uptake experiments by these well-characterized vesicles showed that glucose was taken up in excess of that amount predicted on the basis of the encapsulated aqueous volume. In contrast, the equilibrium uptake of carnitine can be predicted solely on the basis of the vesicle encapsulated volume. Each excess glucose molecule was found to be associated with from 7 to 5200 phospholipid molecules for 100 and 0.1 mM glucose, respectively. Uptake of glucose by PC-PA-Chol vesicles is independent of the cholesterol concentration and is similar to that observed in PC-PA vesicles. The cholesterol concentration independence and oil/buffer partitioning studies with octane and octanol, coupled with previous studies, strongly suggest that excess glucose is located in the vicinity of the phospholipid head group. A probable mechanism would have phospholipid, water and glucose all involved in the interaction rather than a competition between water and glucose for the phospholipid surface, as has been suggested in the literature. 相似文献
2.
In our previous paper, it was reported that peimine, peiminine and a new alkaloid, Zhebeinine, were isolated from the bulbs of Fritillaria thunbergii Miq. In consecutive investigation of the plant, an additional new alkloid, 5α, 14α-cevanine-3β-hydroxy-6-one, named zhebei rine(2) and a known eduardine (1) were isolated and the structure of zhebeirine was determined on the basis of spectral analysis. Eduardine(1) was firstly isolated from the bulbs. 相似文献
3.
T C Lee M L Blank C Piantadosi K S Ishaq F Snyder 《Biochimica et biophysica acta》1975,409(2):218-224
An unnatural phospholipid, phosphatidyl-N-isopropylethanolamine, was isolated from rat liver after intraperitoneal injections of N-isopropylethanol-amine; it was identified on the basis of enzymic, chemical, and chromatographic analyses. Although this phospholipid was formed at the expense of phosphatidylcholine and phosphatidylethanolamine, its fatty acid composition did not resemble either of these lipids. Microsomes, mitochondria, and plasma membranes contained significant amounts (up to 9%) of this unusual phospholipid. Radioisotope incorporation experiments suggest that the N-isopropylethanol-amine containing phospholipid is rapidly equilibrated between microsomes and mitochondria and more slowly with surface membranes. 相似文献
4.
C S Lai J Joseph C C Shih 《Biochemical and biophysical research communications》1989,160(3):1189-1195
We have synthesized a spin-labeled derivative of ET-18-OCH3, a known antitumor ether-linked phospholipid. The spin-labeled analog was shown to be as potent as ET-18-OCH3 in inhibiting 3H-thymidine uptake of HL60 leukemic cells. Electron spin resonance (ESR) studies showed that the mobility of this ether-linked phospholipid in the membrane is more restricted when compared to its ester-linked counterparts. It is probable that the absence of the bulky carbonyl oxygens allows closer packing of the two alkyl chains in the ether-linked phospholipid, thereby reducing the angular amplitude of the motion of the alkyl chains. These findings may be of importance in elucidating mechanisms by which the antitumor ether-linked phospholipids perturb the structure of cellular membranes. 相似文献
5.
Saccharomyces cerevisiae mutant with a partial defect in the synthesis of CDP-diacylglycerol and altered regulation of phospholipid biosynthesis. 总被引:5,自引:3,他引:2
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L S Klig M J Homann S D Kohlwein M J Kelley S A Henry G M Carman 《Journal of bacteriology》1988,170(4):1878-1886
A Saccharomyces cerevisiae mutant (cdg1 mutation) was isolated on the basis of an inositol excretion phenotype and exhibited pleiotropic deficiencies in phospholipid biosynthesis. Genetic analysis of the mutant confirmed that the cdg1 mutation represents a new genetic locus and that a defect in a single gene was responsible for the Cdg1 phenotype. CDP-diacylglycerol synthase activity in mutant haploid cells was 25% of the wild-type derepressed level. Biochemical and immunoblot analyses revealed that the defect in CDP-diacylglycerol synthase activity in the cdg1 mutant was due to a reduced level of the CDP-diacylglycerol synthase Mr-56,000 subunit rather than to an alteration in the enzymological properties of the enzyme. This defect resulted in a reduced rate of CDP-diacylglycerol synthesis, an elevated phosphatidate content, and alterations in overall phospholipid synthesis. Unlike wild-type cells, CDP-diacylglycerol synthase was not regulated in response to water-soluble phospholipid precursors. The cdg1 lesion also caused constitutive expression of inositol-1-phosphate synthase and elevated phosphatidylserine synthase. Phosphatidylinositol synthase was not affected in the cdg1 mutant. 相似文献
6.
Phospholipase D and its application in biocatalysis 总被引:4,自引:0,他引:4
Phospholipase D (PLD) from plants or microorganisms is used as biocatalyst in the transformation of phospholipids and phospholipid analogs in both laboratory and industrial scale. In recent years the elucidation of the primary structure of many PLDs from several sources, as well as the resolution of the first crystal structure of a microbial PLD, have yielded new insights into the structural basis and the catalytic mechanism of this catalyst. This review summarizes some new results of PLD research in the light of application. 相似文献
7.
Suwalsky M Schneider C Villena F Norris B Cárdenas H Cuevas F Sotomayor CP 《Zeitschrift für Naturforschung. C, Journal of biosciences》2001,56(7-8):614-622
The interaction of the local anesthetic dibucaine with the isolated toad skin and membrane models is described. The latter consisted of human erythrocytes, isolated unsealed human erythrocyte membranes (IUM), large unilamellar vesicles (LUV) of dimyristoylphosphatidylcholine (DMPC) and phospholipid multilayers built-up of DMPC and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. Results indicate a significant decrease in the potential difference (PD) and in the short-circuit current (Isc) after the application of dibucaine in toad skin, which may be interpreted as reflecting inhibition of the active transport of ions. This finding might be explained on the basis of the results obtained from fluorescence spectroscopy and X-ray diffraction studies on membrane models. In fact, dibucaine induced structural perturbations in IUM, DMPC LUV and phospholipid multilayers. Scanning electron microscopy revealed that dibucaine induced erythrocyte stomatocytosis. According to the bilayer couple hypothesis an echinocytic type of shape change would have been expected given the preferential interaction of dibucaine with DMPC. Although it is still premature to define the molecular mechanism of action of dibucaine, the experimental results confirm the important role played by the phospholipid bilayers in the association of the anesthetic with cell membranes. 相似文献
8.
W R Rice V K Sarin J L Fox J Baatz S Wert J A Whitsett 《Biochimica et biophysica acta》1989,1006(2):237-245
To determine whether small hydrophobic surfactant peptides (SP-B and SP-C) participate in recycling of pulmonary surfactant phospholipid, we determined the effect of these peptides on transfer of 3H- or 14C-labelled phosphatidylcholine from liposomes to isolated rat alveolar Type II cells and Chinese hamster lung fibroblasts. Both natural and synthetic SP-B and SP-C markedly stimulated phosphatidylcholine transfer to alveolar Type II cells and Chinese hamster lung fibroblasts in a dose- and time-dependent fashion. Effects of the peptides on phospholipid uptake were dose-dependent, but not saturable and occurred at both 4 and 37 degrees C. Uptake of labelled phospholipid into a lamellar body fraction prepared from Type II cells was augmented in the presence of SP-B. Neither SP-B nor SP-C augmented exchange of labelled plasma membrane phosphatidylcholine from isolated Type II cells or enhanced the release of surfactant phospholipid when compared to liposomes without SP-B or SP-C. Addition of native bovine SP-B and SP-C to the phospholipid vesicles perturbed the size and structure of the vesicles as determined by electron microscopy. To determine the structural elements responsible for the effect of the peptides on phospholipid uptake, fragments of SP-B were synthesized by solid-phase protein synthesis and their effects on phospholipid uptake assessed in Type II epithelial cells. SP-B (1-60) stimulated phospholipid uptake 7-fold. A smaller fragment of SP-B (15-60) was less active and the SP-B peptide (40-60) failed to augment phospholipid uptake significantly. Like SP-B and SP-C, surfactant-associated protein (SP-A) enhanced phospholipid uptake by Type II cells. However, SP-A failed to significantly stimulate phosphatidylcholine uptake by Chinese hamster lung fibroblasts. These studies demonstrate the independent activity of surfactant proteins SP-B and SP-C on the uptake of phospholipid by Type II epithelial cells and Chinese hamster lung fibroblasts in vitro. 相似文献
9.
In our previous papers, it was reported that peimine, peiminine, eduardine and three new alkaloids, zhebeinine, zhebeirine, zhebeinone, were isolated from the bulbs of Fritillaria thunbergii Miq. In consecutive investigation of the plant, an additional new alkaloidal glucoside, 5α , 14α -cevanine-6α , 20β-dihydroxyl-3β-O-β-D-glucoside, named zhebeininoside (Ⅰ) and aknown picropodophyllotoxin (Ⅱ) were isolated. The structure of zhebeininoside was determined on the basis of spectral analysis and chemical reaction. Picropodophyllotoxin was firstly isolated from Fritillaria plants. 相似文献
10.
Refolding and proton pumping activity of a polyethylene glycol-bacteriorhodopsin water-soluble conjugate. 总被引:1,自引:0,他引:1
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Bacteriorhodopsin (BR), from the purple membrane (PM) of Halobacterium halobium, was chemically modified with methoxypolyethylene glycol (m-PEG; molecular weight = 5,000 Da) succinimidyl carbonate. The polyethylene glycol-bacteriorhodopsin (m-PEG-SC-BR33) conjugate, containing one polyethylene glycol chain, was water soluble. The secondary structure of the conjugate in water appeared partially denatured, but was shown to contain alpha-helical segments by circular dichroism spectroscopy. The isolated bacteriorhodopsin conjugate, with added retinal, was refolded in a mixed detergent-lipid micelle and had an absorption maximum at 555 nm. The refolded conjugate was transferred into vesicles that pumped protons, upon illumination, as efficiently as did native BR. Modification of the PM with m-PEG did not alter the native structure or inhibit proton pumping, and therefore it is suggested that the glycol polymer is present as a moiety covalently linked to residues unnecessary for proton pumping and proper folding. The site of attachment of m-PEG was determined to be at either Lys 129 or Lys 159, with position Lys 129 the most probable site of attachment. The m-PEG-SC-BR33 could be stepwise refolded to the native conformation by the addition of trifluoroethanol to lower the dielectric constant, simulating the insertion of the BR into the phospholipid bilayer. 相似文献
11.
Phospholipids were isolated from mitochondrial, microsomal, and plasma membranes of LM cells and fractionated into individual phospholipid classes on silicic acid columns. The fatty acid composition and the rotational relaxation time of 1,6-diphenyl-1,3,5-hexatriene (DPH) were determined for each phospholipid class. Sphingomyelin was the only phospholipid isolated from LM cell membranes that showed a phase transition within the temperature range investigated, 5-40 degrees C. The rotational relaxation times for DPH were lowest in phosphatidylcholine in all the membrane fractions. Phosphatidylcholine isolated from the three membrane fractions of choline-supplemented cells had similar rotational relaxation times and phosphatidylcholine isolated from microsomal membranes of linoleate-supplemented cells had lower rotational relaxation times. The results indicate that the differences in the rotational relaxation times of DPH between mitochondrial, microsomal, and plasma membrane phospholipids could be explained primarily by differences in the polar head-group composition, while differences in the fatty acid composition had only a minor effect. This provides a basis for understanding how the different lipid components in these cells contribute to membrane fluidity. 相似文献
12.
Suwalsky M Schneider C Villena F Norris B Cárdenas H Cuevas F Sotomayor CP 《Biophysical chemistry》2005,116(3):227-235
The interaction of the local anesthetic procaine with human erythrocytes, isolated unsealed human erythrocyte membranes (IUM), isolated toad skins, and molecular models is described. The latter consisted of phospholipid multilayers built-up of dimyristoylphosphatidylcholine (DMPC) and of dimyristoylphosphatidylethanolamine (DMPE), representatives of phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. Optical and scanning electron microscopy of human erythrocytes revealed that procaine induced the formation of stomatocytes. Experiments performed on IUM at 37 degrees C by fluorescence spectroscopy showed that procaine interacted with the phospholipid bilayer polar groups but not with the hydrophobic acyl chains. X-ray diffraction indicated that procaine perturbed DMPC structure to a higher extent when compared with DMPE, its polar head region being more affected. Electrophysiological measurements disclosed a significant decrease in the potential difference (PD) and in the short-circuit current (Isc) after the application of procaine to isolated toad skin, reflecting inhibition of active ion transport. 相似文献
13.
千针万线草环肽H的结构 总被引:6,自引:0,他引:6
从云南民间药物千针万线草的新鲜根中又得到2个环肽成分,其中1个为新环肽,命名为千针万线草环肽H。经光谱技术及化学方法证明其结构为cyclo,另一为一已知的环肽化合物。 相似文献
14.
L Herbette J K Blasie P Defoor S Fleischer R J Bick W B Van Winkle C A Tate M L Entman 《Archives of biochemistry and biophysics》1984,234(1):235-242
The total phospholipid content and distribution of phospholipid species between the outer and inner monolayers of the isolated sarcoplasmic reticulum membrane was measured by phospholipase A2 activities and neutron diffraction. Phospholipase measurements showed that specific phospholipid species were asymmetric in their distribution between the outer and inner monolayers of the sarcoplasmic reticulum lipid bilayer; phosphatidylcholine (PC) was distributed 48/52 +/- 2% between the outer and inner monolayer of the sarcoplasmic reticulum bilayer, 69% of the phosphatidyl-ethanolamine (PE) resided mainly in the outer monolayer of the bilayer, 85% of the phosphatidylserine (PS) and 88% of the phosphatidylinositol (PI) were localized predominantly in the inner monolayer. The total phospholipid distribution determined by these measurements was 48/52 +/- 2% for the outer/inner monolayer of the sarcoplasmic reticulum lipid bilayer. Sarcoplasmic reticulum phospholipids were biosynthetically deuterated and exchanged into isolated vesicles with both a specific lecithin and a general exchange protein. Neutron diffraction measurements directly provided lipid distribution profiles for both PC and the total lipid content in the intact sarcoplasmic reticulum membrane. The outer/inner monolayer distribution for PC was 47/53 +/- 1%, in agreement with phospholipase measurements, while that for the total lipid was 46/54 +/- 1%, similar to the phospholipase measurements. These neutron diffraction results regarding the sarcoplasmic reticulum membrane bilayer were used in model calculations for decomposing the electron-density profile structure (10 A resolution) of isolated sarcoplasmic reticulum previously determined by X-ray diffraction into structures for the separate membrane components. These structure studies showed that the protein profile structure within the membrane lipid bilayer was asymmetric, complementary to the asymmetric lipid structure. Thus, the total phospholipid asymmetry obtained by two independent methods was small but consistent with a complementary asymmetric protein structure, and may be related to the highly vectorial functional properties of the calcium pump ATPase protein in the sarcoplasmic reticulum membrane. 相似文献
15.
Annonaceous acetogenins from the leaves of Annona montana 总被引:6,自引:0,他引:6
Wang LQ Min BS Li Y Nakamura N Qin GW Li CJ Hattori M 《Bioorganic & medicinal chemistry》2002,10(3):561-565
A novel Annonaceous acetogenin, montanacin F, with a new type of terminal lactone unit, was isolated from the leaves of Annona montana. Its structure was determined on the basis of spectral evidences and chemical methods, and a possible biosynthetic pathway was discussed. In addition, the cytotoxicity of montanacin F was evaluated in vitro against Lewis lung carcinoma (LLC) tumor cell lines. Furthermore, the previously isolated cytotoxic acetogenin annonacin against LLC was examined for in vivo antitumor activity with LLC tumor cells. 相似文献
16.
本文以红细胞膜为材料,通过测量磷脂过氧化荧光产物的产生,磷脂组分的损伤以及膜蛋白二级结构的破坏,内源荧光的下降和蛋白SDS-凝胶电泳分析,探讨了甲素光敏作用中蛋白和磷脂的损伤。结果表明:甲素存在时红细胞膜样品照光,使磷脂产生过氧化荧光产物和丙二醛,磷脂组分受到破坏(其中PE与PS较敏感),在膜状态的磷脂比提取的磷脂脂质体中的损伤来得剧烈。同时,膜蛋白二级结构遭到破坏,内源荧光下降。在膜状态中spectrin的损伤比提取出的spectrin的损伤来得严重。据此,我们认为,在甲素光敏作用产生的蛋白和磷脂的损伤过程中,蛋白与磷脂间存在着相互作用和相互影响,使损伤加剧。 相似文献
17.
竹红菌甲素对红细胞膜蛋白及膜磷脂的光敏损伤 总被引:6,自引:2,他引:4
In this paper, using human erythrocyte membrane, the photodamage of Hypocrellin A to membrane protein and phospholipid was studied by measuring the lipid peroxidation, the damage of phospholipid, the change of protein secondary structure, the endogenous fluorescence change and SDS-polyacrylamide gel electrophoresis analysis. These results showed that illumination of erythrocyte membrane in presence of Hypocrellin A can cause lipid peroxidation producing fluorescence adduct and MDA, decomposing in phospholipid composition in which PE and PS were more sensitive than others. Meanwhile, the secondary structure of membrane protein was destroyed and endogenous fluorescence decreased. The photodamage on phospholipid and spectrin occurred more seriously in the case they were embedded in membrane than they were in isolated form. So we suggest that they are interactions existing between proteins and phospholipids to enhance the damage on protein and phospholipid during the HA-sensitized photodamage on membrane. 相似文献
18.
Mrnka L Nováková O Novák F Tvrzická E Pácha J 《Physiological research / Academia Scientiarum Bohemoslovaca》2000,49(2):197-205
The effect of low-salt diet on phospholipid composition and remodeling was examined in rat colon which represents a mineralocorticoid target tissue. To elucidate this question, male Wistar rats were fed a low-salt diet and drank distilled water (LS, low-salt group) or saline instead of water (HS, high-salt group) for 12 days before the phospholipid concentration and fatty acid composition of isolated colonocytes were examined. The dietary regimens significantly influenced the plasma concentration of aldosterone which was high in LS group and almost zero in HS group. Plasma concentration of corticosterone was unchanged. When expressed in terms of cellular protein content, a significantly higher concentration of phospholipids was found in LS group, with the exception of sphingomyelin (SM) and phosphatidylserine (PS). Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) accounted for more than 70% of total phospholipids in both groups. A comparison of phospholipid distribution in LS and HS groups demonstrated a higher percentage of PE and a small, but significant, decrease of PC and SM in LS group. The percentage of phosphatidylinositol (PI), PS and cardiolipin (CL) were not affected by mineralocorticoid treatment. With respect to the major phospholipids (PE, PC), a higher level of n-6 polyunsaturated fatty acids (PUFA) and lower levels of monounsaturated fatty acids were detected in PC of LS group. The increase of PUFA predominantly reflected an increase in arachidonic acid by 53%. In comparison to the HS group, oleic acid content was decreased in PC and PE isolated from colonocytes of the LS group. Our data indicate that alterations in phospholipid concentration and metabolism can be detected in rats with secondary hyperaldosteronism. The changes in phospholipid concentration and their fatty acid composition during fully developed effect of low dietary Na+ intake may reflect a physiologically important phenomenon with long-term consequences for membrane structure and function. 相似文献
19.
A derivative of Lactobaccillus casei ATCC 7469, characterized by limited growth in liquid media and an unusual phospholipid composition, has been isolated. Grown to early stationary phase on a lipid-free and inositol-free medium, the organism produces phosphatidylinositol phosphatidylglycerol, and diphosphatidylglycerol. The phosphatidylinositol was identified by thin-layer, paper, and gas chromatography, and by mass spectrometry. In agreement with published data, the conventional strain produced phosphatidylglycerol, diphosphatidylglycerol, and lysylphosphatidylglycerol, but no phosphatidylinositol. The phospholipid/glycolipid molar ratio, calculated on the basis of published glycolipid analyses, is 1.3 : 1 for the derivative and 1.5 : 1 for the conventional strain. 相似文献
20.
Experimental studies on antiarrhythmic and antiseizure effects of polyunsaturated fatty acids in excitable tissues 总被引:3,自引:0,他引:3
Leaf A Kang JX Xiao YF Billman GE Voskuyl RA 《The Journal of nutritional biochemistry》1999,10(8):440-448
It has been shown that in animals, and probably in humans, n-3 polyunsaturated fatty acids (PUFAs) are antiarrhythmic. We discuss our recent studies on the antiarrhythmic actions of PUFAs. PUFAs stabilize the electrical activity of isolated cardiac myocytes by requiring a stronger electrical stimulus to elicit an action potential and by markedly prolonging the refractory period. These electrophysiologic effects are the result of specific modulation of ion currents, particularly of the voltage-dependent sodium current and of the L-type calcium currents across sarcolemmal phospholipid membranes. This appears to be the probable major antiarrhythmic mechanism of PUFAs. However, they also similarly affect neuronal ion channels with potentially important functional effects on the nervous system. 相似文献