Morphofunctional parameters and adaptation capabilities of students at the beginning of the third millennium

On the basis of comprehensive anthropometrical observation of 1st and 2nd year students from different faculties of Moscow State University (MSU) carried out in 2002-2003, functional characteristics of the cardiovascular system (systolic and diastolic blood pressure, heart rate) were investigated in 205 young men and 327 young women along with traditional morphological parameters. In comparisons of contemporary young men and women with their peers, whose characteristics were obtained in the course of investigations carried out over the period 1920-1990, secular trends towards an increase of body length and a worsening of strength indices were detected. Evaluation and comparative analysis of adaptation capabilities of students were carried out based on screening and assessment of adaptation potential using the Bayevsky method (1987). It was shown that the parameters of physical development and the level of adaptation of an organism to environmental conditions can be used as additional markers for determination of the health status of contemporary students for early prevention of some diseases, improvement of their physical status and increase of adaptation potentials.     

Symbiosis research at the end of the millenium

The development of symbiosis research over the closing 50 years of the last millenium is reviewed. At the beginning of this period, there had been very little previous research into aquatic microbial symbiosis. The advent of new experimental techniques, combined with the developing acceptance of the symbiotic origin of eukaryotic cell structure (and especially that chloroplasts evolved from a symbiosis involving photosynthetic aquatic microbes) brought symbiosis research into much greater prominence for a time in the 1970s and 1980s. Nevertheless, at the end of the millenuim, symbiosis as a subject still lacks a clear and strong identity amongst biologists in general. Three reasons are identified for this: continuing absence of a generally accepted definition of the term; little or no representation in the academic structure of biology; and the current adverse climate of research funding in many countries. However, the growing importance of symbiosis in biotechnology and in conserving biodiversity makes future prospects much brighter.     

Messenger RNA degradation: beginning at the end

The mechanisms responsible for mRNA decay in mammalian cells, and how specific sequence elements accelerate decay, are unknown. Recent work indicates that 'ARE' instability elements recruit the exosome to promote rapid 3'-to-5' degradation of the mRNA.     

Biophysics at the turn of the new millenium: perfluorocarbon media and gas-transporting blood substitutes

The results of 20-year investigations of perfluorocarbon gas-transporting emulsions for biological and medical applications performed by russian biophysicists together with chemists and clinicists are reviewed. As a result of these investigations, the blood substitute perftoran was created. Now this commercial blood substitute has different applications in clinics of Russia and other countries.     

Laboratory methods of diagnosis of syphilis for the beginning of the third millennium

Despite that the whole genome of T. pallidum, the causative agent of syphilis, has been sequenced, syphilis is, and will remain for some time, diagnosed by direct clinical observation and by laboratory methods. This review presents comprehensively most of the practical techniques used for direct detection of T. pallidum and lists all practical methods for phospholipid and treponemal antibodies detection. It describes most novel tests for syphilis, discusses problems with sero-creossreactivity in Lyme disease, immune responses in HIV-syphilis coinfected patients, and reviews serologic responses to antibiotic treatment.     

Antioxidant defence mechanisms: From the beginning to the end (of the beginning)

When life first evolved on Earth, there was little oxygen in the atmosphere. Evolution of antioxidant defences must have been closely associated with the evolution of photosynthesis and of O₂-dependent electron transport mechanisms. Studies with mice lacking antioxidant defences confirm the important roles of MnSOD and transferrin in maintaining health, but show that glutathione peroxidase (GPX) and CuZnSOD are not essential for everyday life (at least in mice). Superoxide can be cytotoxic by several mechanisms: one is the formation of hydroxyl radicals. There is good evidence that OH^· formation occurs in vivo. Other important antioxidants may include thioredoxin, and selenoproteins other than GPX. Nitric oxide may be an important antioxidant in the vascular system. Diet-derived antioxidants are important in maintaining human health, but recent studies employing “biomarkers” of oxidative DNA damage are questioning the “antioxidant” roles of β-carotene and ascorbate. An important area of future research will be elucidation of the reasons why levels of steady-state oxidative damage to DNA and lipids vary so much between individuals, and their predictive value for the later development of human disease.     

Antioxidant defence mechanisms: From the beginning to the end (of the beginning)

When life first evolved on Earth, there was little oxygen in the atmosphere. Evolution of antioxidant defences must have been closely associated with the evolution of photosynthesis and of O₂-dependent electron transport mechanisms. Studies with mice lacking antioxidant defences confirm the important roles of MnSOD and transferrin in maintaining health, but show that glutathione peroxidase (GPX) and CuZnSOD are not essential for everyday life (at least in mice). Superoxide can be cytotoxic by several mechanisms: one is the formation of hydroxyl radicals. There is good evidence that OH^· formation occurs in vivo. Other important antioxidants may include thioredoxin, and selenoproteins other than GPX. Nitric oxide may be an important antioxidant in the vascular system. Diet-derived antioxidants are important in maintaining human health, but recent studies employing “biomarkers” of oxidative DNA damage are questioning the “antioxidant” roles of β-carotene and ascorbate. An important area of future research will be elucidation of the reasons why levels of steady-state oxidative damage to DNA and lipids vary so much between individuals, and their predictive value for the later development of human disease.