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The helminthological fauna of the cotton rat, Sigmodon hispidus in a tropical environment varies according to habitat and feeding behavior. Six species of nematodes (Longistriata adunca, Trichostrongylus sigmodontis, Strongyloides sigmodontis, Litomosoides carinii, Monodontus sp. and Protospirura sp.) and two species of cestodes (Hymenolepis diminuta and Raillietina sp.) were found in rats from extensive dry lands in Guanacaste where hot temperatures and heterogeneous diet are the rule. Only two species of nematodes (Longistriata adunca and Angiostrongylus costaricensis) were found in rats collected in a humid pineapple plantation in the Central Plateau (Alajuela) where mild temperatures predominate. A. costaricensis, a metastrongylid of medical importance, was found in 42% of them. 相似文献
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L. I. Apfelbaum A. Blanco 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,70(5):569-572
Summary Six population samples of the South American cricetid rodent Akodon dolores were collected at the same site at six-month intervals over a three year period. Changes in density were detected. Seven out of 18 loci analyzed by means of starch gel electrophoresis were polymorphic. Only two of these loci (Est-4 and G6pdh) showed statistically significant variation in allele frequencies following a seasonal pattern. There was no correlation between allele frequencies and population density. When animals were grouped into two classes according to body weight, a clear difference in allele distribution at the Est-4 and G6pdh loci was observed between individuals 39 g or less and those heavier than 39 g. As the first group comprises predominantly younger animals, the data indicate that changes in the age-structure of population, rather than density variations, are responsible for the cyclic pattern of allele frequencies fluctuations. 相似文献
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Jing Li Xin Zheng Yansen Cai Xiuyue Zhang Min Yang Bisong Yue Jing Li 《Molecular ecology resources》2015,15(1):153-167
Identification of rodents is very difficult mainly due to high similarities in morphology and controversial taxonomy. In this study, mitochondrial cytochrome oxidase subunit I (COI) was used as DNA barcode to identify the Murinae and Arvicolinae species distributed in China and to facilitate the systematics studies of Rodentia. In total, 242 sequences (31 species, 11 genera) from Murinae and 130 sequences (23 species, 6 genera) from Arvicolinae were investigated, of which 90 individuals were novel. Genetic distance, threshold method, tree‐based method, online BLAST and BLOG were employed to analyse the data sets. There was no obvious barcode gap. The average K2P distance within species and genera was 2.10% and 12.61% in Murinae, and 2.86% and 11.80% in Arvicolinae, respectively. The optimal threshold was 5.62% for Murinae and 3.34% for Arvicolinae. All phylogenetic trees exhibited similar topology and could distinguish 90.32% of surveyed species in Murinae and 82.60% in Arvicolinae with high support values. BLAST analyses yielded similar results with identification success rates of 92.15% and 93.85% for Murinae and Arvicolinae, respectively. BLOG successfully authenticated 100% of detected species except Leopoldamys edwardsi based on the latest taxonomic revision. Our results support the species status of recently recognized Micromys erythrotis, Eothenomys tarquinius and E. hintoni and confirm the important roles of comprehensive taxonomy and accurate morphological identification in DNA barcoding studies. We believe that, when proper analytic methods are applied or combined, DNA barcoding could serve as an accurate and effective species identification approach for Murinae and Arvicolinae based on a proper taxonomic framework. 相似文献
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A satellite DNA fraction from P. eremicus, having a buoyant density of 1.705 g/ml in neutral CsCl density gradients, was isolated. In situ hybridization experiments, using 3H-RNA complementary to this DNA fraction indicated that the short (heterochromatic) arms of most of the autosomes contained this sequence. Conversely, in situ hybridization using 3H-complementary DNA (cDNA) synthesized from the cytoplasmic poly (A) RNA of P. eremicus (comprising a substantial fraction of total messenger RNA) showed that the number of silver grains in the long arms (euchromatin) was significantly higher than that in the short arms. The X chromosomes showed a distinct localization pattern of both sequences. 相似文献
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Buoyant density in CsCl, melting temperature, and G + C base content of the DNA from four species of Akodon (Rodentia, Cricetidae) were determined. The buoyant density values of 1.699–1.701 g/cm3 were in accordance with the data reported for other cricetids. No satellite bands were seen in neutral CsCl. The T
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values determined in 1 × SSC ranged from 86.2 to 87.0 C, which corresponds to G + C contents of 41.2–43.2%. There was good agreement in DNA base composition of the four species, although values were slightly higher in A. obscurus, suggesting a certain degree of interspecies variability.This study was supported by grants from Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, and Organización de Estados Americanos. 相似文献
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Genetic similarity between species of rodents of the Akodon genus (A. dolores, A. molinae, and A. azarae) has been estimated by analysis of electrophoretic zymograms corresponding to 23 loci. Nei's coefficient between A. dolores and A. molinae was within the range usually found in conspecific populations. This evidence plus the successful production of "hybrids" (Merani et al., J. Exp. Zool., 206:343-346, '78) suggests that A. dolores and A. molinae may represent geographic races of the same species. 相似文献
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N. O. Bianchi C. Redi C. Garagna E. Capanna M. G. Manfredi-Romanini 《Journal of molecular evolution》1983,19(5):362-370
Nuclear DNA contents were estimated by microdensitometry in five species of Akodon rodents: Arodon molinae, A. dolores, A. mollis, A. azarae, Bolomys obscurus) and in three chromosomal varieties of A. molinae (2n = 42; 2n = 43, 2n = 22). The data obtained showed that the species with the highest DNA content was B. obscurus, followed in order of decreasing genome size by A. molinae, A. mollis, A. dolores and A. azarae. In A. molinae the forms with 2n = 42 chromosomes had the lowest and the forms with 2n = 44 the highest amount of DNA, while the forms with 2n = 43 had intermediate DNA contents. The variation in DNA amount detected in A. molinae was interpreted as a phenomenon of amplification occurring in the chromosomal areas involved in the chromosomal rearrangement giving rise to the polymorphism exhibited by this species. The DNA contents of shared chromosomes (chromosomes with similar size, morphology and G banding pattern, which are found in two or more phylogenetically related species), were compared and correlated with values of total nuclear DNA. The information obtained indicates that: (a) shared chromosomes have variable amounts of DNA: (b) in a given species there is a correlation between the amount of nuclear and chromosomal DNA in most shared chromosomes (and perhaps in most of the chromosomal complement), e.g., the higher the amount of nuclear DNA, the higher the content of DNA in shared chromosomes; (c) some chromosomes may undergo processes of amplification or deletion restricted to certain regions and usually related with mechanisms of chromosomal rearrangements.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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The climbing rats (Tylomys spp.) have diploid numbers of 52 (T. panamensis), 42 (T. nudicaudus), 40 (T. n. gymnurus) and 36 (T. n. villai). Using G-band analysis we found that the variations are mainly of the Bobertsonian type, and practically all changes can be traced. G-banding also revealed that biarmed chromosomes with similar morphology may be composed of different components. Such conclusions were verified also by analysis of the chromosomes of interspecific hybrids. C-band staining showed that the constitutive heterochromatin of Tylomys is mainly located in the centromeric regions and the sex chromosomes, a situation similar to that of Microtus agrestis. In one specimen of T. panamensis, however, an additional terminal heterochromatic segment was found in one member of the large metacentric pair. Our data underline that in mammalian cytotaxonomy studies both C- and G-band (or C- and Q-band) techniques must be applied to gain maximal information. 相似文献
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Chromosomal pairing and chiasma formation were studied two individuals of Peromyscus beatae heterozygous for the presence of a large block of interstitial heterochromatin. Although the modified chromosome was of medium size, analysis of C-banded diakinetic configurations revealed that it was the homolog of one of the smallest autosomes. Analysis of silver stained synaptonemal complexes indicated that synapsis was either unidirectional from initiation at one set of telomeres or was bidirectional from initiation at both sets of telomeres. Each pattern resulted in characteristic heteromorphic pairing configurations (interstitial asynapsis or terminally positioned unpaired segments) in early pachynema. These configurations underwent synaptic adjustment and, by mid-pachynema, the lateral elements of the polymorphic bivalent either appeared typical of homomorphic bivalents or exhibited regional heteropycnosis in one or both axes. Synaptonemal complex data for Peromyscus and many other mammalian species reflect an apparent need for fully paired, linear bivalents prior to the end of pachynema. 相似文献
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The husbandry and breeding of Calomys laucha (Rodentia, Cricetidae) in captivity are described. Growth curves based on body weight and length showed statistical differences between sexes after 45 days, males being heavier than females. The overall reproductive efficiency was 53.4% but birth rate was depressed during winter. Gestation length was 21 +/- 1 days and females exhibited postpartum oestrus with a 3-7 day implantation delay (51%). Litter size was 5.3 +/- 1.1 (n = 34). Pup survival at weaning was 84.9%. Mean life span in laboratory conditions was 13.5 months and a cumulative mortality of 90% was reached at 27-28 months of age. 相似文献
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Christelle Tougard Sophie Montuire Vitaly Volobouev Evgenia Markova Julien Contet Vladimir Aniskin Jean‐Pierre Quere 《Biological journal of the Linnean Society. Linnean Society of London》2013,108(2):434-452
Natural hybridization between species is not a rare event. In arvicoline rodents, hybridization is known to occur in the wild and/or in captivity. In the Microtus arvalis group, cytogenetic studies revealed that there were two distinct chromosomal forms (2n = 46 but a different fundamental number of autosomes). These forms have been attributed to two cryptic species: the common (arvalis) and Altai (obscurus) voles. Recently, individuals with intermediate karyotypes (F1 and backcrosses) were discovered in central European Russia, and, for this reason, other studies have regarded obscurus and arvalis as conspecific. In the present study, to address the question of the species limits in the Altai vole and to infer its evolutionary history, a phylogeographical analysis combined with multivariate morphometric methods and original chromosome data was performed. Two obscurus lineages were identified: the Sino‐Russian and South Caucasian lineages. Both lineages are characterized by low genetic diversity, resulting, in the former, from a past bottleneck event caused by encroaching periglacial areas and, in the latter, from recent rapid population divergence. Introgressive hybridization between the Altai and common voles appears to be the result of a secondary contact following the Last Glacial Maximum in central European Russia. Despite the fact that speciation is an ongoing process in most arvicoline species, the common and Altai voles are genetically divergent, morphologically and karyologically distinct, and exhibit contrasting evolutionary histories. For all these reasons, they should be ranked as species: M. arvalis and M. obscurus. © 2013 The Linnean Society of London 相似文献
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NAD-linked lactate, malate, glycerophosphate, alcohol and nonspecific dehydrogenases, aspartate aminotransferases, and soluble esterases from extracts of tissues of individuals from a wild population of Calomys musculinus (Rodentia, Cricetidae) have been analyzed by means of starch gel electrophoresis and specific staining. Allelic frequencies and heterozygosity have been determined. Mendelian inheritance of some of the variants detected was confirmed by breeding experiments. Ten out of fifteen (66.6%) of the genetic loci investigated presented polymorphism. Mean heterozygosity per locus was very high (H=0.2014, se 0.046).This work has been supported, in part, by grants from the Secretaria de Ciencia y Tecnología de la Nación (National Program for Endemic Diseases) and from the Fundación Emilio Ocampo. C. N. G. is a Fellow and A. B. a Career Investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas of Argentina. 相似文献
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Early taxonomic investigations associated the genus Scotinomys with South American akodontine rodents, but more recent morphological analyses based in large part on the glans penis have linked brown mice with North American peromyscines, specifically the genus Baiomys. Differentially stained chromosomal preparations of S. xerampelinus were compared with other cricetine taxa. Chromosomally, golden mice (Ochrotomys) were found to be most similar to Scotinomys, this association based on two apparent synapomorphic G-banded chromosomes. The majority of banded chromosomes possessed by Scotinomys were found to be either uniquely derived or to appear unaltered, and therefore presumably ancestral, when compared to the G-band patterns of other cricetids. These results fail to support the morphological hypotheses that closely unite Scotinomys with Baiomys, and instead support a hypothesis that treats Scotinomys, Ochrotomys, and perhaps Baiomys as a rather loosely associated assemblage of genera that are phylogenetically intermediate between the relatively complex pene, primarily South American cricetine rodents and the more simple pene North American neotomine-peromyscines. 相似文献