A Review of: “Biological Recognition and Assembly,D. S. Eisenberg,J. A. Lake and C. F. Fox,Eds. Alan R. Liss,New York, 1980; hardbound, 355 pages, $52.00”

Two MMP-7-ase isoenzymes were purified 100-fold from rat muscle extract to apparent homogeneity, with an overall yield of 10%, using homogenization, ultracentrifugation, high-performance aqueous size-exclusion and high-performance anion exchange chromatography methods. When using a TSK G-2000SW column, the separation resulted in a 6-fold purification and 30% recovery of isoenzymes B and C. This concentrated enzyme extract was then passed through a TSK-DEAE-2SW column, using salt gradient at pH 7.5, with an additional 25-fold purification and 90% recovery of the isoenzymes. Two symmetrical enzyme peaks, representing isoenzymes B and C, were detected when performing purity tests of the active enzymes on the anion exchanger and reversed-phase HFLC columns. The procedures involved are extraction, ultracentri-fugation, chromatographies and enzyme assays and require less than five hours.     

关于南雄盆地K/T界线问题的讨论──与D.A.拉塞尔,D.E.拉塞尔,A.R.斯威特商榷

陆相山间盆地的沉积过程远比某些海相的沉积过程复杂。评述了当今流行的利用沉积速率来检验、甚至推断陆相磁性地层极性时序列方法的可靠性。南雄盆地地层及哺乳动物化石的分布研究表明,前人所定Ｋ／Ｔ界线上下地层连续,并不象一些学者认为的在上湖组底部缺失了数百万年的沉积。