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A GSK3/shaggy-like kinase (AtGSK1) has been implicated in the regulation of drought and salt tolerance. We transferred AtGSK1 from Arabidopsis thaliana to a hybrid poplar (Populus alba × P. tremula var. grandulosa) to determine the effect of the transgene expression in the transgenic trees. The results from northern blot and RT-PCR analyses showed that the expression level varied among the transgenic lines. During their culture on tissue culture media, the transgenic poplars formed vigorous growing roots even in the presence of 125 mM NaCl and callus in the presence of 150 mM NaCl. When the transgenic poplars were growing in pots and provided with NaCl solution, they stayed much healthier than did nontransgenic poplars, showing higher rates of photosynthetic rates, stomatal conductance, and evaporation rates under the stress. Whereas the total level of leaf Na+ level increased dramatically in transgenic poplars under severe saline conditions (150 mM NaCl), that of leaf K+ decreased in the same plants under the same conditions. Total root Na+ level increased in nontransgenic poplars under severe saline conditions. In contrast, total root K+ level decreased in the same plants under the same conditions. The chloride content and relative electrical conductivity of the transgenic poplars after salt stress treatment were lower than those of nontransgenic poplars. The transgenic poplars were also tolerant to up to 20 % PEG remaining significantly healthy when compared with nontransgenic poplars with necrosis and chlorosis symptoms. Another dramatic feature of the transgenic poplars was wilting tolerance for prolonged drought treatment up to 2 weeks. The results provide evidence that the expression of AtGSK1 gene conferred drought and salt tolerance in the transgenic poplars. 相似文献
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Ivanova LA Ronzhina DA Ivanov LA Stroukova LV Peuke AD Rennenberg H 《Plant biology (Stuttgart, Germany)》2011,13(4):649-659
Recent studies of transgenic poplars over‐expressing the genes gsh1 and gsh2 encoding γ‐glutamylcysteine synthetase (γ‐ECS) and glutathione synthetase, respectively, provided detailed information on regulation of GSH synthesis, enzymes activities and mRNA expression. In this experiment, we studied quantitative parameters of leaves, assimilating tissues, cells and chloroplasts, mesophyll resistance for CO2 diffusion, chlorophyll and carbohydrate content in wild‐type poplar and transgenic plants over‐expressing gsh1 in the cytosol after 3 years of growth in relatively clean (control) or heavy metal‐contaminated soil in the field. Over‐expression of gsh1 in the cytosol led to a twofold increase of intrafoliar GSH concentration and influenced the photosynthetic apparatus at different levels of organisation, i.e., leaves, photosynthetic cells and chloroplasts. At the control site, transgenic poplars had a twofold smaller total leaf area per plant and a 1.6‐fold leaf area per leaf compared to wild‐type controls. Annual aboveground biomass gain was reduced by 50% in the transgenic plants. The reduction of leaf area of the transformants was accompanied by a significant decline in total cell number per leaf, indicating suppression of cell division. Over‐expression of γ‐ECS in the cytosol also caused changes in mesophyll structure, i.e., a 20% decrease in cell and chloroplast number per leaf area, but also an enhanced volume share of chloroplasts and intercellular airspaces in the leaves. Transgenic and wild poplars did not exhibit differences in chlorophyll and carotenoid content of leaves, but transformants had 1.3‐fold fewer soluble carbohydrates. Cultivation on contaminated soil caused a reduction of palisade cell volume and chloroplast number, both per cell and leaf area, in wild‐type plants but not in transformants. Biomass accumulation of wild‐type poplars decreased in contaminated soil by more than 30‐fold, whereas transformants showed a twofold decrease compared to the control site. Thus, poplars over‐expressing γ‐ECS in the cytosol were more tolerant to heavy metal stress under field conditions than wild‐type plants according to the parameters analysed. Correlation analysis revealed strong dependence of cell number per leaf area unit, chloroplast parameters and mesophyll resistance with the GSH level in poplar leaves. 相似文献
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Assembly of a cytosolic pine glutamine synthetase holoenzyme in leaves of transgenic poplar leads to enhanced vegetative growth in young plants 总被引:2,自引:0,他引:2
J. FU R. SAMPALO F. GALLARDO F. M. CÁNOVAS & E. G. KIRBY 《Plant, cell & environment》2003,26(3):411-418
Over‐expression of glutamine synthetase (GS, EC 6.3.1.2), a key enzyme in nitrogen assimilation, may be a reasonable approach to enhance plant nitrogen use efficiency. In this work phenotypic and biochemical characterizations of young transgenic poplars showing ectopic expression of a pine cytosolic GS transgene in photosynthetic tissue (Gallardo et al., Planta 210, 19–26, 1999) are presented. Analysis of 22 independent transgenic lines in a 6 month greenhouse study indicated that expression of the pine GS transgene affects early vegetative growth and leaf morphology. In comparison with non‐transgenic controls, transgenic trees exhibited significantly greater numbers of nodes and leaves (12%), and higher average leaf length and width resulting in an increase in leaf area (25%). Leaf shape was not altered. Transgenic poplars also exhibited increased GS activity (66%), chlorophyll content (33%) and protein content (21%). Plant height was correlated with GS content in young leaves, suggesting that GS can be considered a marker for vegetative growth. Molecular and kinetic characterization of GS isoforms in leaves indicated that poplar GS isoforms are similar to their counterparts in herbaceous plants. A new GS isoenzyme that displayed molecular and kinetic characteristics corresponding to the octomeric pine cytosolic GS1 was identified in the photosynthetic tissues of transgenic poplar leaves. These results indicate that enhanced growth and alterations in biochemistry during early growth are the consequence of transgene expression and assembly of pine GS1 subunits into a new functional holoenzyme in the cytosol of photosynthetic cells. 相似文献
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Ting Liao Shiping Cheng Xiaohu Zhu Yu Min Xiangyang Kang 《Trees - Structure and Function》2016,30(4):1137-1147
Key message
The growth vigor of Populus triploid groups explained by higher photosynthetic rate in the vertical canopy gradient, higher relative chlorophyll content, and larger leaf area, compared to diploid group.Abstract
Polyploids show vegetative growth superiority compared to diploids, however, the reason remains unclear. Here, we explored this observation based on variations in 12 phenotypic traits including vegetative growth, leaf area, and photosynthesis using 120 genotypes with three allotriploid groups of different heterozygosities (obtained using three types of 2n gametes) and one diploid group obtained from the same parents in Populus. Wide ranges in phenotypic variation (2.70–38.34 %) were detected in all traits within the progeny population. In addition, the vegetative growth traits, net photosynthetic rate (Pn), relative chlorophyll content index (CCI), leaf area (LA), and photosynthetic efficiency of whole leaves (PEw) in the polyploid group were significantly higher than those in the diploid group, indicating that certain polyploid groups had greater advantages in these respects. However, there were also significant differences in vegetative growth, Pn, LA, and PEw among the three allopolyploid groups, which probably resulted from the 2n gametes with different origins transferring different heterozygosities. Furthermore, a higher Pn of vertical canopy gradient photosynthesis was observed in triploid groups compared to the diploid group. In general, the greater vegetative growth advantages in relation to photosynthesis in the triploid groups were explained by three reasons including a higher Pn which probably resulted from a higher CCI, a higher PEw mainly caused by a larger LA, and a lower aging rate of mature leaves.7.
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以野生型(WT)和转正义叶绿体单脱氢抗坏血酸还原酶基因(LeMDAR)番茄为试材,探讨了UV-B胁迫下过表达LeMDAR对番茄抗氧化能力的影响。测定了不同时间uV-B处理下番茄抗坏血酸(AsA)含量,脱氢抗坏血酸(DHA)含量,单脱氢抗坏血酸还原酶(MDAR)活性,光合速率和叶绿素荧光参数等。在UV-B处理下,转基因番茄植株的AsA含量、MDAR酶及抗坏血酸过氧化物酶(APx)活性、H:0:和超氧阴离子清除速率、净光合速率(只)高于野生型番茄。此外,紫外胁迫下,转基因株系丙二醛(MDA)含量和相对电导率(REC)较野生型增加的少。上述结果表明,MDAR对抗抗坏血酸再生具有重要作用,过表达LeMDAR提高了番茄植株抗氧化能力,对光合机构有保护作用。 相似文献
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Weidong Wang Yuhua Wang Yulin Du Zhen Zhao Xujun Zhu Xin Jiang Zaifa Shu Ying Yin Xinghui Li 《Plant cell reports》2014,33(11):1829-1841
Key message
Overexpression of CsHis in tobacco promoted chromatin condensation, but did not affect the phenotype. It also conferred tolerance to low-temperature, high-salinity, ABA, drought and oxidative stress in transgenic tobacco.Abstract
H1 histone, as a major structural protein of higher-order chromatin, is associated with stress responses in plants. Here, we describe the functions of the Camellia sinensis H1 Histone gene (CsHis) to illustrate its roles in plant responses to stresses. Subcellular localization and prokaryotic expression assays showed that the CsHis protein is localized in the nucleus, and its molecular size is approximately 22.5 kD. The expression levels of CsHis in C. sinensis leaves under various conditions were investigated by qRT-PCR, and the results indicated that CsHis was strongly induced by various abiotic stresses such as low-temperature, high-salinity, ABA, drought and oxidative stress. Overexpression of CsHis in tobacco (Nicotiana tabacum) promoted chromatin condensation, while there were almost no changes in the growth and development of transgenic tobacco plants. Phylogenetic analysis showed that CsHis belongs to the H1C and H1D variants of H1 histones, which are stress-induced variants and not the key variants required for growth and development. Stress tolerance analysis indicated that the transgenic tobacco plants exhibited higher tolerance than the WT plants upon exposure to various abiotic stresses; the transgenic plants displayed reduced wilting and senescence and exhibited greater net photosynthetic rate (Pn), stomatal conductance (Gs) and maximal photochemical efficiency (Fv/Fm) values. All the above results suggest that CsHis is a stress-induced gene and that its overexpression improves the tolerance to various abiotic stresses in the transgenic tobacco plants, possibly through the maintenance of photosynthetic efficiency. 相似文献10.
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Meiru Li Xiaojie Lin Hongqing Li Xiaoping Pan Guojiang Wu 《Plant Cell, Tissue and Organ Culture》2011,107(2):283-293
Overexpression of NHX genes has been previously shown to improve salt tolerance of transgenic plants. In this study, transgenic rice plants overexpressing AtNHX5 showed not only high salt tolerance, but also high drought tolerance. Measurements of ion levels indicated that Na+ and K+ contents were all higher in AtNHX5 overexpressing shoots than in wild type (WT) shoots in high saline conditions. After exposure to water deficiency and salt stress, the WT plants all died, while the AtNHX5 overexpressing rice plants had a higher survival rate, dry weight, leaf water content, and leaf chlorophyll contents, accumulated more proline, and had less membrane damage than the WT plants. In addition, seeds of both transgenic and WT plants germinated on 1/2 MS medium supplemented with 250 mM mannitol, but overexpression of AtNHX5 improved the shoot growth of the seedlings. Taken together, the results indicate that AtNHX5 gene could enhance the tolerance of rice plants to multiple environmental stresses by promoting the accumulation of more effective osmolytes (ions or proline) to counter the osmotic stress caused by abiotic factors. 相似文献
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超表达拟南芥2-烯醛还原酶基因对烟草抗旱性的作用机理分析 总被引:1,自引:0,他引:1
为研究是否可以利用2-烯醛还原酶(AER)来清除活性氧下游的醛自由基达到提高植物的抗旱性,以超表达拟南芥AER基因烟草和野生型烟草(SR)为研究材料,利用干旱胁迫处理进行抗旱性分析,测定了干旱胁迫及复水后各个烟草株系的生物量、光合速率、叶绿素荧光参数、叶绿素含量、MDA和H2O2含量等指标。结果显示:(1)干旱胁迫下,转基因烟草株系的生物量、叶绿素含量、净光合速率、PSⅡ最大光化学效率及H2O2的清除能力均显著高于对照;(2)复水之后,烟草植株的各项生理指标都得到一定程度的恢复,而转基因株系相比于野生型恢复迅速,恢复能力更强。研究认为,超表达AER基因可以通过清除活性氧及其下游醛自由基来提高烟草的抗旱能力。 相似文献
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Youzhi Li Hongyan Qin Yonghong Xie Wei Wang Xinsheng Chen Canming Zhang 《Wetlands Ecology and Management》2014,22(1):25-33
The use of large-scale tree plantations has provoked increasing concern regarding the negative effects on local environments in different ecosystems. However, the physiological mechanism underlying the reduction in soil water by tree plantations in wetlands is not clear. The aims of this study were to investigate the effects of poplar (Populus deltoides) plantations on soil water content and to elucidate the underlying physiological mechanisms. To this end, we conducted a 1-year fixed-plot investigation of soil water content (SWC), plant photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), and water-use efficiency (WUE) of individual leaves of 11- and 5-year-old poplars and of reed (Triarrherca sacchariflora, a native herbaceous plant) in the Dongting Lake wetlands, China. SWC was highest in reed, intermediate in 11-year-old poplar, and lowest in 5-year-old poplar, suggesting that poplar plantations produce a lower soil water content in wetlands. From May to July, Pn was significantly higher in reed than in the two poplar stands, but did not differ between the different-aged poplars. As a whole, Gs and Tr were higher, but WUE was lower, in the poplar stands than in reed during the growing season, indicating that Gs and Tr are the key physiological mechanisms associated with the lower soil water in poplar stands. Relationships among Pn, Gs, and Tr showed positive correlations (P < 0.01) for each type of vegetation. These data suggest that poplar plantations may cause the transformation of wetlands into dry land due to a lower WUE leading to a massive water loss from soil. This, in turn, would have an influence on community composition and ecosystem function after establishment of the plantations. 相似文献
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Damaraju S Schlede S Eckhardt U Lokstein H Grimm B 《Journal of plant physiology》2011,168(12):1444-1451
Functional aspects of water soluble chlorophyll-binding protein (WSCP) in plants were investigated during the courses of leaf senescence, chlorophyll biogenesis, stress response and photoprotection. The cDNA sequence encoding WSCP from cauliflower was cloned into a binary vector to facilitate Agrobacterium tumefaciens mediated transformation of Nicotiana tabacum. The resultant transgenic tobacco plants overexpressed the CauWSCP gene under the control of a 35S-promoter. Analyses of protein and pigment contents indicate that WSCP overexpression does not enhance chlorophyll catabolism in vivo, thus rendering a role of WSCP in Chl degradation unlikely. Accumulation of higher levels of protochlorophyllide in WSCP overexpressor plants corroborates a proposed temporary storage and carrier function of WSCP for chlorophyll and late precursors. Although WSCP overexpressor plants did not show significant differences in non-photochemical quenching of chlorophyll fluorescence, they are characterized by significantly lower zeaxanthin accumulation and peroxidase activity at different light intensities, even at high light intensities of 700-900 μmol photons m−2 s−1. These results suggest a photoprotective function of the functional chlorophyll binding-WSCP tetramer by shielding of chlorophylls from molecular oxygen. 相似文献
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为了探讨番茄GDP—L-半乳糖磷酸酶对烟草抗坏血酸(AsA)含量及抗氧化能力的影响,从番茄叶片中分离了GDP-L-半乳糖磷酸酶基因(LeGGP),并转入到烟草中。以野生型(WT)和转正义LeGGP烟草株系T1-3和T1-15为试材,测定了甲基紫精(MV)处理下AsA、脱氢抗坏血酸(DHA)、H2O2、O2-和叶绿素含量、抗坏血酸过氧化物酶(APX)活性、光合速率和叶绿素荧光参数等。Northem杂交分析表明LeGGP的表达受MV的诱导,在MV处理下,野生型烟草的离体叶圆片发生比转基因烟草更严重的光漂白,转基因烟草的AsA含量及清除H2O2和O2-的能力明显强于野生型,过表达LePGG胀高了烟草的生长量。并且转基因烟草比野生型具有更高的净光合效率(Pn)和光系统Ⅱ(PSII)最大光化学效率(眠)。结果表明,LeGGP的过表达有助于提高烟草AsA含量及抗氧化胁迫能力。 相似文献
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5-aminolevulinic acid (ALA) is a key precursor for the biosynthesis of porphyrins such as heme and chlorophyll. ALA alleviates salinity stress damage in germinating seeds and improves seedling growth. Exogenous application of ALA at low concentrations has been shown to enhance salt tolerance in a number of plants. In the present study, we studied the effect of exogenous application of ALA on enhancing salt stress tolerance in Isatis indigotica Fort. (Anhui population as S1, Shanxi population as S2). A foliar application of 0, 12.5, 16.7, 25.0, and 50.0 mg/L ALA was given to the leaves of I. indigotica plants treated with 100 mmol/L NaCl. The fresh weight of leaves and roots; chlorophyll relative content (SPAD value); photosynthetic parameters, such as net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular carbon dioxide concentration (Ci) and water use efficiency of the treated plants were determined. The third leaf of each treated plant was used to determine the activities of antioxidant enzymes. Superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutamate synthase (GOGAT), nitrate reductase (NR) activities and the malondialdehyde (MDA) content increased in response to 100 mmol/L NaCl in both S1 and S2 plants. However, the fresh weight of leaf and root, chlorophyll relative content, Pn, Gs, Ci decreased in response to salt stress in both S1 and S2 plants. In all foliar application of ALA in S1 plants, the MDA content, and the activities of SOD and POD were the highest in response to 50.0 mg/L foliar application of ALA. GOGAT and NR activities were the highest in response to 16.7 mg/L foliar ALA. Chlorophyll content and Pn were the highest in S1 plants treated with by 25.0 mg/L ALA. In S2 plants, plant fresh weight, chlorophyll relative content, SOD, CAT, NR activities and Pn treated with 16.7 mg/L ALA were higher than that of the control (CK0). POD, MDA, GOGAT activities in S2 plants treated with 25.0 mg/L ALA were the highest among all treatments. Thus, our results showed that the optimal concentration of ALA (16.7 ~ 25.0 mmol/L) increases the activity of antioxidant enzymes, which in turn helps to abate the damage caused by salt stress in I. indigotica seedlings. Furthermore, ALA also results in an increase in chlorophyll content, Pn and the activities of GOGAT and NR. 相似文献
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Overexpression of the rubisco activase gene improves growth and low temperature and weak light tolerance in Cucumis sativus 下载免费PDF全文
Rubisco activase (RCA) is an important enzyme that can catalyze the carboxylation and oxygenation activities of ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco), which is involved in the photosynthetic carbon reduction cycle. Here, we studied the effects of changes in RCA activity on photosynthesis, growth and development, as well as the low temperature and weak light tolerance of RCA overexpressing transgenic cucumber (Cucumis sativus) plants. CsRCA overexpression increased the plant height, leaf area and dry matter, and decreased the root/top ratio in transgenic cucumber plants compared with the wild‐type (WT) plants. Low temperature and low light stress led to decreases in the CsRCA expression and protein levels, the photosynthetic rate (Pn) and the stomatal conductance (Gs), but an increase in the intercellular CO2 (Ci) concentration in cucumber leaves. The actual photochemical efficiency and maximal photochemical efficiency of photosystem II in cucumber seedlings also declined, but the initial fluorescence increased during low temperature and weak light stress. Transgenic plants showed a lower decrease in the CsRCA expression level and actual and maximal photochemical efficiencies, as well as increases in the Ci and initial fluorescence relative to the WT plants. Low temperature and low light stress resulted in a significant increase in the malondialdehyde (MDA) content; however, this increase was reduced in transgenic plants compared with that in WT plants. Thus, the overexpression of CsRCA may promote the growth and low temperature and low light tolerance of cucumber plants in solar greenhouses. 相似文献
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Fumiaki Funahashi Seiichi Ohta Toru Taniguchi Manabu Kurita Ken-ichi Konagaya Takahisa Hayashi 《Trees - Structure and Function》2014,28(1):65-76