Organization of Multisynaptic Inputs to the Dorsal and Ventral Dentate Gyrus: Retrograde Trans-Synaptic Tracing with Rabies Virus Vector in the Rat

Behavioral, anatomical, and gene expression studies have shown functional dissociations between the dorsal and ventral hippocampus with regard to their involvement in spatial cognition, emotion, and stress. In this study we examined the difference of the multisynaptic inputs to the dorsal and ventral dentate gyrus (DG) in the rat by using retrograde trans-synaptic tracing of recombinant rabies virus vectors. Three days after the vectors were injected into the dorsal or ventral DG, monosynaptic neuronal labeling was present in the entorhinal cortex, medial septum, diagonal band, and supramammillary nucleus, each of which is known to project to the DG directly. As in previous tracing studies, topographical patterns related to the dorsal and ventral DG were seen in these regions. Five days after infection, more of the neurons in these regions were labeled and labeled neurons were also seen in cortical and subcortical regions, including the piriform and medial prefrontal cortices, the endopiriform nucleus, the claustrum, the cortical amygdala, the medial raphe nucleus, the medial habenular nucleus, the interpeduncular nucleus, and the lateral septum. As in the monosynaptically labeled regions, a topographical distribution of labeled neurons was evident in most of these disynaptically labeled regions. These data indicate that the cortical and subcortical inputs to the dorsal and ventral DG are conveyed through parallel disynaptic pathways. This second-order input difference in the dorsal and ventral DG is likely to contribute to the functional differentiation of the hippocampus along the dorsoventral axis.     

Motor organization of the lateral cerebellar nucleus of the rat

The motor organization of the nucleus lateralis (NL) of the cerebellum of the rat was investigated by studying the motor effects following the electrical microstimulation. The movements evoked by the NL stimulation concerned prevalently the forelimb and the head segments. The movements of the hindlimb segments were evoked in only few cases. The NL is organized as a mosaic of zones without, or at least very little overlap. The various body segments are differently represented in the NL. Some of them are once represented (single representations). In other cases, the same movements were evoked by different NL regions (multiple representations). Finally, in a last lot of cases, various representations concerned the same body regions but from each representation a different type of movement was evoked (specific representations, i.e. displacement of an individual digit and flexion of all digits together). The topographical distribution of the representations in the NL cytological regions (magnicellularis, NLm; dorsolateral hump, DLH; subnucleus lateralis parvocellularis, slp) suggests the idea that each of them may be concerned in a specific motor activity: the NLm would control the position of the body, or of part of it, in the space; the DLH would be concerned in the oral (prevalently) and in the forelimb motor activity; the slp would be concerned in the exploration of the environment as well as in skilled movements of the distalmost forelimb segments.     

Some neuroanatomical aspects of primate locomotor evolution

Primate locomotor patterns are the result of osteological and myological interactions, the effectiveness of which is governed by various afferent, internuncial and efferent central nervous system pathways. The distribution of primary afferents following lumbosacral ganglionectomies and the distribution of corticospinal fibers following lesion of contralateral motor cortex to medial and lateral ventral horn motor nuclei have been discussed for the tree shrew and bushbaby. Based on limited data it has been suggested that the tree shrew is the best living example of the quadrupedal Paleocene forms from which primates presumably evolved, and the bushbaby is one of the best examples of the vertical clingers and leapers which appeared in the Eocene. Both forms have dense primary afferent distribution to cells of the lateral portion of the ventral horn, which are related to appendicular musculature, and sparse projection to the medial part of the ventral horn which innervates axial musculature. Corticospinal fibers in the tree shrew do not synapse in any portion of the ventral horn at any spinal cord level. The bushbaby has direct cortical motor control over cells located in the medial portion of the ventral horn and, consequently, over axial musculature. Extrapolations from studies on the tree shrew and other generalized forms suggested that Paleocene quadrupeds lacked cortical control over axial and appendicular musculature and depended primarily on subcorticospinal pathways and spinal reflexes for the execution of their locomotor pattern. Eocene vertical clingers and leapers retained the reflex pathways of their Paleocene ancestors but acquired direct cortical motor control over axial musculature, thus indicating a first level of neuroanatomical adaptation related to evolving locomotor styles. It was suggested that there are correlations between locomotor style and neuromorphological specializations, and that comparative observations on key extant phylogenetic forms may provide a clearer and more complete picture of initial locomotor adaptations in the primate series.     

Dynamic Neural Network Models of the Premotoneuronal Circuitry Controlling Wrist Movements in Primates

Dynamic recurrent neural networks were derived to simulate neuronal populations generating bidirectional wrist movements in the monkey. The models incorporate anatomical connections of cortical and rubral neurons, muscle afferents, segmental interneurons and motoneurons; they also incorporate the response profiles of four populations of neurons observed in behaving monkeys. The networks were derived by gradient descent algorithms to generate the eight characteristic patterns of motor unit activations observed during alternating flexion-extension wrist movements. The resulting model generated the appropriate input-output transforms and developed connection strengths resembling those in physiological pathways. We found that this network could be further trained to simulate additional tasks, such as experimentally observed reflex responses to limb perturbations that stretched or shortened the active muscles, and scaling of response amplitudes in proportion to inputs. In the final comprehensive network, motor units are driven by the combined activity of cortical, rubral, spinal and afferent units during step tracking and perturbations.The model displayed many emergent properties corresponding to physiological characteristics. The resulting neural network provides a working model of premotoneuronal circuitry and elucidates the neural mechanisms controlling motoneuron activity. It also predicts several features to be experimentally tested, for example the consequences of eliminating inhibitory connections in cortex and red nucleus. It also reveals that co-contraction can be achieved by simultaneous activation of the flexor and extensor circuits without invoking features specific to co-contraction.     

The Corticocuneate Pathway in the Cat: Relations among Terminal Distribution Patterns,Cytoarchitecture, and Single Neuron Functional Properties

A combined anatomical and physiological strategy was used to investigate the organization of the corticocuneate pathway in the cat. The distribution of the corticocuneate projection was mapped by means of the anterograde horseradish peroxidase (HRP) labeling technique and correlated with the nuclear cytoarchitecture in Nissl and Golgi material, the distribution of retrogradely labeled relay cells after HRP injections in the ventrobasal complex of the thalamus, and the topographic organization derived from single-and multiunit recordings in the decerebrate, unanesthetized cat. This approach provided details about the arrangement of the corticocuneate pathway that were not available from previous studies with anterograde degeneration methods.

On the basis of cytoarchitectonic and connectional features, a number of subdivisions are identified in the cuneate nucleus, each of which is associated with characteristic functional properties. In agreement with previous studies, it is found that a large portion of the cuneate nucleus, the middle dorsal part (MCd), is exclusively devoted to the representation of cutaneous receptive fields on the digits. This “core” region contains more thalamic projecting neurons than any other subdivision of the cuneate nucleus. A topographic arrangement also exists in the subdivisions of the rostral cuneate and of the nuclear region ventral to MCd, although in these, receptive fields are larger and predominantly, but not exclusively, related to deep receptors and involve the arm, shoulder, and trunk.

Observations on corticocuneate projections were based on injections, mainly focused on functional subdivisions of the primary somatosensory cortex (SI) as described by McKenna et al (1981). Although cortical projections are mainly to cuneate regions other than its core, a significant proportion of fibers from the region of SI where the digits are represented (particularly area 3b) do project to the MCd region of the cuneate nucleus. Similarly, nuclear areas associated with receptive fields on the arm and trunk are labeled after injection in SI arm and trunk regions, respectively. Thus, a close topographic relationship appears to exist between the somatosensory cortex and cuneate regions related to the same body representation, although nuclear regions in which receptive fields on the neck area are represented receive very sparse or no detectable cortical projections even when the injection of the tracer involves the entire sensorimotor cortex. The topographic arrangement of SI projections upon the cuneate nucleus suggests that a similar pattern exists in both structures with regard to the relative representations of distal versus proximal and deep versus cutaneous receptive fields (e.g., “core” vs. “shell” organization), and that cuneate regions preferentially related to either of these classes of receptive fields receive direct connections from the corresponding regions in SI.

A comparison of the results from cats with tracer injections in areas 4 and 3b reveals that the projections from the former is denser than that arising from the latter and that their territories of termination largely overlap in the ventral portions of the cuneate nucleus. However, cortical projections to MCd may be derived from the somatosensory cortex with no contribution from area 4. The demonstration of the relative selectivity of cortical projections from different cytoarchitectonic and functional cortical areas to cuneate regions identified here provides a structural basis for the elucidation of the physiological and behavioral observations, particularly on cortical modulation of somatosensory transmission during movements.     

Distinct cortical circuit mechanisms for complex forelimb movement and motor map topography

Cortical motor maps are the basis of voluntary movement, but they have proven difficult to understand in the context of their underlying neuronal circuits. We applied light-based motor mapping of Channelrhodopsin-2 mice to reveal a functional subdivision of the forelimb motor cortex based on the direction of movement evoked by brief (10?ms) pulses. Prolonged trains of electrical or optogenetic stimulation (100-500?ms) targeted to anterior or posterior subregions of motor cortex evoked reproducible complex movements of the forelimb to distinct positions in space. Blocking excitatory cortical synaptic transmission did not abolish basic motor map topography, but the site-specific expression of complex movements was lost. Our data suggest that the topography of?movement maps arises from their segregated output projections, whereas complex movements evoked by prolonged stimulation require intracortical synaptic transmission.     

Changes in electrical thresholds for evoking movements from the cat cerebral cortex following lesions of the sensori-motor area

We evaluated motor maps in the cerebral cortex and motor performance in cats before and after lesions of the forelimb representation in the primary motor area. After the lesion there was a reduction in the use of the affected forelimb and loss of accuracy in prehension tasks using the forelimb; some recovery occurred during the mapping study. Electrode tracts and lesion sites were located in cytoarchitectonically identified cortical areas 4gamma, 4delta, 6aalpha, 6agamma, 3a. The lesions were mainly in area 4gamma. In the lesioned hemisphere there were many points around the lesion site (in areas 4gamma and 3a) from which movements could not be evoked. In some areas distant from the lesion site (e.g. area 6agamma) the mean thresholds for evoking forelimb movements were significantly elevated. Mean thresholds for evoking hindlimb and facial movements were not different from before. In the contralateral hemisphere mean thresholds for evoking forelimb, but not hindlimb or facial movements, were significantly elevated in several sensorimotor areas (area 4gamma, 6agamma and 3a). Mean thresholds for evoking forelimb movements appeared to progressively increase during the time of study. Minimal currents required to evoke forelimb movements from the cerebral cortex increase (possibly progressively) following a lesion of the forelimb representation in the primary motor area, affecting many interconnected motor areas in the hemispheres ipsilateral and contralateral to the lesioned site. This increase in thresholds may play a role in the changes in cortical control of the affected and contralateral limbs following brain lesions and explain the increased sense of effort required to produce movements.     

Changes in electrical thresholds for evoking movements from the cat cerebral cortex following lesions of the sensori-motor area

We evaluated motor maps in the cerebral cortex and motor performance in cats before and after lesions of the forelimb representation in the primary motor area. After the lesion there was a reduction in the use of the affected forelimb and loss of accuracy in prehension tasks using the forelimb; some recovery occurred during the mapping study. Electrode tracts and lesion sites were located in cytoarchitectonically identified cortical areas 4γ, 4δ, 6aα, 6aγ, 3a. The lesions were mainly in area 4γ. In the lesioned hemisphere there were many points around the lesion site (in areas 4γ and 3a) from which movements could not be evoked. In some areas distant from the lesion site (e.g. area 6aγ) the mean thresholds for evoking forelimb movements were significantly elevated. Mean thresholds for evoking hindlimb and facial movements were not different from before. In the contralateral hemisphere mean thresholds for evoking forelimb, but not hindlimb or facial movements, were significantly elevated in several sensorimotor areas (area 4γ, 6aγ and 3a). Mean thresholds for evoking forelimb movements appeared to progressively increase during the time of study. Minimal currents required to evoke forelimb movements from the cerebral cortex increase (possibly progressively) following a lesion of the forelimb representation in the primary motor area, affecting many interconnected motor areas in the hemispheres ipsilateral and contralateral to the lesioned site. This increase in thresholds may play a role in the changes in cortical control of the affected and contralateral limbs following brain lesions and explain the increased sense of effort required to produce movements.     

The distribution of neural crest-derived Schwann cells from subsets of brachial spinal segments into the peripheral nerves innervating the chick forelimb.

Neural crest cells from brachial levels of the neural tube populate the ventral roots, spinal nerves, and peripheral nerves of the chick forelimb where they give rise to Schwann cells. The distribution of neural crest cells in the developing forelimb was examined using homotopic and heterotopic chick-quail chimeras to label neural crest cells from subsets of the brachial spinal segments. Neural crest cells from particular regions of the spinal cord populated ventral roots and spinal nerves adjacent to or immediately posterior to the graft. Crest cells also populated the brachial plexus in accord with their segmental origins. In the forelimb, neural crest cells populated muscle nerves with anterior brachial spinal segments populating nerves to anterior musculature of the forelimb and posterior brachial spinal segments populating nerves to posterior musculature. Similar patterns were seen following both homotopic and heterotopic transplantation. In both types of grafts, the distribution of neural crest cells largely matched the sensory and motor projection pattern from the same spinal segmental level. This suggests that neural crest-derived Schwann cells from a particular spinal segment may use sensory and motor fibers emerging from the same segmental level as substrates to guide their migration into the periphery.     

Study Motor Skill Learning by Single-pellet Reaching Tasks in Mice

Reaching for and retrieving objects require precise and coordinated motor movements in the forelimb. When mice are repeatedly trained to grasp and retrieve food rewards positioned at a specific location, their motor performance (defined as accuracy and speed) improves progressively over time, and plateaus after persistent training. Once such reaching skill is mastered, its further maintenance does not require constant practice. Here we introduce a single-pellet reaching task to study the acquisition and maintenance of skilled forelimb movements in mice. In this video, we first describe the behaviors of mice that are commonly encountered in this learning and memory paradigm, and then discuss how to categorize these behaviors and quantify the observed results. Combined with mouse genetics, this paradigm can be utilized as a behavioral platform to explore the anatomical underpinnings, physiological properties, and molecular mechanisms of learning and memory.     

Three-dimensional electrophysiological topography of the rat corticostriatal system

Projections from the cerebral cortex are the major afferents of the caudoputamen and probably determine the functions subserved by each region of the nucleus. The corticostriatal system has been mapped using cytological techniques which give little information on the physiological importance of projections from individual cortical areas. The objective of this study was to characterize the three-dimensional topography of the corticostriatal system in the rat and to determine the physiological significance of these projections using electrophysiological techniques. Eight functionally distinct areas of the cerebral cortex (prefrontal, primary motor, rostral and caudal primary somatosensory, hindlimb, auditory, occipital and primary visual) were stimulated while recording the multiple unit activity in seven dorsal and seven ventral areas of the caudoputamen. Each stimulation site produced a distinctive pattern of activation within the caudoputamen. There was also a large site-dependent variation in electrophysiological activation produced by each stimulation. The motor and somatosensory areas produced the most powerful overall activation. In addition, a number of trends were obvious. There was a rostrocaudal topographical relationship between the site of stimulation and the area of the caudoputamen activated. Furthermore, more caudally and medially placed stimulation sites produced greater dorsal activation of the caudoputamen relative to ventral.     

Spatial organization of inhibitory control of activity of vestibulospinal Deiters' neurons by Purkinje cells in the anterior lobe of the cerebellum

The topographical distribution of vestibulospinal neurons in Deiters' nucleus was investigated by a microelectrode method. By contrast with observations made in morphological experiments, the localization of antidromically identified vestibulocervical (C-neurons) and vestibulolumbar (L-neurons) cells was found not to be limited to the ventral middle and rostral third of the nucleus (the forelimb region) and caudodorsal part of the nucleus (hind limb region), but to include the whole of the ventral and dorsal half of the nucleus, respectively. The zones of localization of these two groups of neurons are not confined to a single layer: C-neurons are found in the dorsal half of the nucleus and L-neurons in its ventrocaudal part also. Analysis of the distribution of monosynaptic IPSPs arising in response to activation of Purkinje cells in the vestibulospinal neurons showed that C-neurons are controlled chiefly from the forelimb zone of the cerebellar cortex whereas L-neurons are controlled equally by inhibitory influences from the forelimb and hind limb zones of the anterior lobe of the cerebellar cortex.L. A. Orbeli Institute of Physiology, Academy of Sciences of the Armenian SSR, Erevan. Translated from Neirofiziologiya, Vol. 11, No. 1, pp. 54–64, January–February, 1979.     

The modulation of sensory transmission through the medullary dorsal horn during cortically driven mastication

During mastication, reflexes are modulated and sensory transmission is altered in interneurons and ascending pathways of the rostral trigeminal sensory complex. The current experiment examines the modulation of sensory transmission through the most caudal part of the trigeminal sensory system, the medullary dorsal horn, during fictive mastication produced by cortical stimulation. Extracellular single unit activity was recorded from the medullary dorsal horn, and multiple unit activity was recorded from the trigeminal motor nucleus in anesthetized, paralyzed rabbits. The masticatory area of sensorimotor cortex was stimulated to produce rhythmic activity in the trigeminal motor nucleus (fictive mastication). Activity in the dorsal horn was compared in the presence and absence of cortical stimulation. Fifty-two percent of neurons classified as low threshold and 83% of neurons receiving noxious inputs were influenced by cortical stimulation. The cortical effects were mainly inhibitory, but 21% of wide dynamic range and 6% of low threshold cells were excited by cortical stimulation. The modulation produced by cortical stimulation, whether inhibitory or excitatory, was not phasically related to the masticatory cycle. It is likely that, when masticatory movements are commanded by the sensorimotor cortex, the program includes tonic changes in sensory transmission through the medullary dorsal horn.     

Connections of thalamic nucleus lateralis posterior with suprasylvian cortex in cats

Cats were immobilized with D-tubocurarine. Responses of 231 neurons of the thalamic nucleus lateralis posterior to cortical stimulation in areas 5b and 21 of the suprasylvian gyrus were studied. Responses of 34 neurons were antidromic, indicating the existence of a direct projection of this nucleus to the cortical areas studied. This projection was most extensive in area 5b. The long latencies (up to 60 msec) of the antidromic responses of some neurons indicate that axons of certain neurons of thalamic nucleus lateralis posterior conduct excitation very slowly (0.3 m/sec). Orthodromic responses with latencies of 2–3 msec to cortical stimulation point to the presence of direct pathways from cortex to nucleus. The flow of afferent impulses into the nucleus from area 5b is stronger than from area 21. Convergence of impulses from these areas was observed on 44% of neurons of the nucleus. Cortical stimulation of areas 5b and 21 evoked postsynaptic inhibition in most neurons of the nucleus. It is concluded that two-way direct connections exist between nucleus lateralis posterior of the thalamus and the suprasylvian cortex.     

Dopamine Promotes Motor Cortex Plasticity and Motor Skill Learning via PLC Activation

Dopaminergic neurons in the ventral tegmental area, the major midbrain nucleus projecting to the motor cortex, play a key role in motor skill learning and motor cortex synaptic plasticity. Dopamine D1 and D2 receptor antagonists exert parallel effects in the motor system: they impair motor skill learning and reduce long-term potentiation. Traditionally, D1 and D2 receptor modulate adenylyl cyclase activity and cyclic adenosine monophosphate accumulation in opposite directions via different G-proteins and bidirectionally modulate protein kinase A (PKA), leading to distinct physiological and behavioral effects. Here we show that D1 and D2 receptor activity influences motor skill acquisition and long term synaptic potentiation via phospholipase C (PLC) activation in rat primary motor cortex. Learning a new forelimb reaching task is severely impaired in the presence of PLC, but not PKA-inhibitor. Similarly, long term potentiation in motor cortex, a mechanism involved in motor skill learning, is reduced when PLC is inhibited but remains unaffected by the PKA inhibitor. Skill learning deficits and reduced synaptic plasticity caused by dopamine antagonists are prevented by co-administration of a PLC agonist. These results provide evidence for a role of intracellular PLC signaling in motor skill learning and associated cortical synaptic plasticity, challenging the traditional view of bidirectional modulation of PKA by D1 and D2 receptors. These findings reveal a novel and important action of dopamine in motor cortex that might be a future target for selective therapeutic interventions to support learning and recovery of movement resulting from injury and disease.     

A Comparison of the Ipsilateral Cortical Projections to the Dorsal and Ventral Subdivisions of the Macaque Premotor Cortex

The cortical connections of the dorsal (PMd) and ventral (PMv) subdivisions of the premotor area (PM, lateral area 6) were studied in four monkeys (Macaca fascicularis) through the use of retrograde tracers. In two animals, tracer was injected ventral to the arcuate sulcus (PMv), in a region from which forelimb movements could be elicited by intracortical microstimulation (ICMS). Tracer injections dorsal to the arcuate sulcus (PMd) were made in two locations. In one animal, tracer was injected caudal to the genu of the arcuate sulcus (in caudal PMd [cPMd], where ICMS was effective in eliciting forelimb movements); in another animal, it was injected rostral to the genu of the arcuate sulcus (in rostral PMd [rPMd], where ICMS was ineffective in eliciting movements). Retrogradely labeled neurons were counted in the ipsilateral hemisphere and located in cytoarchitectonically identified areas of the frontal and parietal lobes. Although both PMv and PMd were found to receive inputs from other motor areas, the prefrontal cortex, and the parietal cortex, there were differences in the topography and the relative strength of projections from these areas.

There were few common inputs to PMv and PMd; only the supplementary eye fields projected to all three areas studied. Interconnections within PMd or PMv appeared to link hindlimb and forelimb representations, and forelimb and face representations; however, connections between PMd and PMv were sparse. Areas cPMd and PMv were found to receive inputs from other motor areas—the primary motor area, the supplementary motor area, and the cingulate motor area—but the topography and strength of projections from these areas varied. Area rPMd was found to receive sparse inputs, if any, from these motor areas. The frontal eye field (area 8a) was found to project to PMv and rPMd, and area 46 was labeled substantially only from rPMd. Parietal projections to PMv were found to originate from a variety of somatosensory and visual areas, including the second somatosensory cortex and related areas in the parietal operculum of the lateral sulcus, as well as areas 5, 7a, and 7b, and the anterior intraparietal area. By contrast, projections to cPMd arose only from area 5. Visual areas 7m and the medial intraparietal area were labeled from rPMd. Relatively more parietal neurons were labeled after tracer injections in PMv than in PMd. Thus, PMv and PMd appear to be parts of separate, parallel networks for movement control.     

Comparison of electrical thresholds for evoking movements from sensori-motor areas of the cat cerebral cortex and its relation to motor training

Motor maps and electrical thresholds for evoking movements from motor areas of the cerebral cortex were evaluated in normal cats by using intracortical microstimulation techniques. Stainless steel chambers were implanted over craniotomies in adult cats trained to perform reaching and retrieval movements with their forelimbs. Prehensile motor training was continued and movement performance monitored for about 6-10 weeks during which the cortex was progressively explored with sharp tungsten electrodes inserted into cortical gyri (anterior and posterior sigmoid, and coronal) and the banks of sulci (cruciate, presylvian and coronal). Twice weekly, under light general anaesthesia, 3-4 tracks were made in either hemisphere till about 50 tracks were made in each hemisphere. Mean thresholds for evoking forelimb movements from different cytoarchitectonic areas (4gamma, 4delta, 6agamma and 3a) were compared and no consistent or significant differences were observed between the different areas. In the animals (4/6) which used either forelimb to perform the tasks, there were no consistent differences in the mean thresholds for evoking forelimb movements from the two hemispheres. However, in 2 animals, which used their right forelimbs predominantly or exclusively to perform all the tasks, mean thresholds for evoking forelimb movements was significantly higher in areas 4gamma and 6agamma of the left hemisphere (compared to the right); no consistent differences in the mean thresholds for evoking hindlimb or facial movements were observed between the two hemispheres. These findings suggest that ICMS thresholds for evoking forelimb movements may be similar in different sensorimotor areas of the cat cerebral cortex, and these thresholds could be influenced by motor training.     

Localization of Motor Neurons and Central Pattern Generators for Motor Patterns Underlying Feeding Behavior in Drosophila Larvae

Motor systems can be functionally organized into effector organs (muscles and glands), the motor neurons, central pattern generators (CPG) and higher control centers of the brain. Using genetic and electrophysiological methods, we have begun to deconstruct the motor system driving Drosophila larval feeding behavior into its component parts. In this paper, we identify distinct clusters of motor neurons that execute head tilting, mouth hook movements, and pharyngeal pumping during larval feeding. This basic anatomical scaffold enabled the use of calcium-imaging to monitor the neural activity of motor neurons within the central nervous system (CNS) that drive food intake. Simultaneous nerve- and muscle-recordings demonstrate that the motor neurons innervate the cibarial dilator musculature (CDM) ipsi- and contra-laterally. By classical lesion experiments we localize a set of CPGs generating the neuronal pattern underlying feeding movements to the subesophageal zone (SEZ). Lesioning of higher brain centers decelerated all feeding-related motor patterns, whereas lesioning of ventral nerve cord (VNC) only affected the motor rhythm underlying pharyngeal pumping. These findings provide a basis for progressing upstream of the motor neurons to identify higher regulatory components of the feeding motor system.     

Comparison of electrical thresholds for evoking movements from sensori-motor areas of the cat cerebral cortex and its relation to motor training

Motor maps and electrical thresholds for evoking movements from motor areas of the cerebral cortex were evaluated in normal cats by using intracortical microstimulation techniques. Stainless steel chambers were implanted over craniotomies in adult cats trained to perform reaching and retrieval movements with their forelimbs. Prehensile motor training was continued and movement performance monitored for about 6–10 weeks during which the cortex was progressively explored with sharp tungsten electrodes inserted into cortical gyri (anterior and posterior sigmoid, and coronal) and the banks of sulci (cruciate, presylvian and coronal). Twice weekly, under light general anaesthesia, 3–4 tracks were made in either hemisphere till about 50 tracks were made in each hemisphere. Mean thresholds for evoking forelimb movements from different cytoarchitectonic areas (4γ, 4δ, 6aγ and 3a) were compared and no consistent or significant differences were observed between the different areas. In the animals (4/6) which used either forelimb to perform the tasks, there were no consistent differences in the mean thresholds for evoking forelimb movements from the two hemispheres. However, in 2 animals, which used their right forelimbs predominantly or exclusively to perform all the tasks, mean thresholds for evoking forelimb movements was significantly higher in areas 4γ and 6aγ of the left hemisphere (compared to the right); no consistent differences in the mean thresholds for evoking hindlimb or facial movements were observed between the two hemispheres. These findings suggest that ICMS thresholds for evoking forelimb movements may be similar in different sensorimotor areas of the cat cerebral cortex, and these thresholds could be influenced by motor training.     

Brief communication: Proportions of the ventral half of the cerebellar dentate nucleus in humans and great apes

In a previous study about volume comparisons of the cerebellar complex in some hominoid species (1997), progressive development of only the lateral zone group of nuclei was found in the human cerebellar complex. This development was considered to be related not to bipedalism, but to versatile and coordinated finger movement, evolving after bipedalism was established. It was also considered a prerequisite for the evolution of human language. The lateral zone groups of nuclei are represented by the dentate nucleus. Therefore, the present study reports the development of the dentate nucleus in humans in comparison with that in some great apes. One finding is that the average value of ratios for nucleus size of the ventral half (v) to the dorsal half (d) (v/d) was found to be 2.11 in humans, while it was 1.64 in great apes. This finding shows that the greater part of progressive development of the dentate nucleus in humans is due to the development of its ventral half. Therefore, the fiber connection to the frontal association area from the cerebellar cortex, which is involved in the performance of higher cerebellar functions such as cognitive and language functions, would be mediated by the ventral half of the dentate nucleus.