Effect of cobalt and copper complexes with o-phenanthroline on the respiratory activity of mitochondria]

The effects of cobalt and copper complexes with o-phenantroline on the respiratory activity of mitochondria from pea sprouts and submitochondrial particles from bovine heart and on the oxidative phosphorylation in mitochondria were studied. The catalytic activity of the complexes in several components of the respiratory chain autooxidation reactions was investigated. It was shown that the bis (o-phenantroline) cobalt (II) chloride complex is more active in exidation of NADH. The tris (o-phenantroline) cobolt (III) perchlorate complex stimulates the respiratory activity of mitochondria and submitochondrial particles. Possible localization of the effect of this complex was postulated. The (o-phenantroline) copper chloride complex completely inhibits the succinate-dependent respiration of submitochondrial particles and causes disturbances in oxidative phosphorylation of mitochondria.     

Trace element uptake by L-cells as a function of trace elements in a synthetic growth medium

The concentration of trace elements in L-cells has been studied as a function of the trace metal content of the growth medium. Cells were cultured in synthetic media which contained varying trace amounts of the elements manganese, iron, cobalt, copper, zinc and molybdenum. The cellular concentration of the elements potassium, iron, copper and zinc were then determined. It was found that the cell accumulates trace metals at a different rate than they are made available. Deficiencies in zinc could be “induced” in the cell by increasing the concentration of iron, manganese and cobalt; cellular iron deficiencies were observed at larger medium concentrations of zinc, manganese, copper and cobalt. Trace metal uptake by the cell was seen to parallel the utilization by multicellular organisms.     

Influence of soil waterlogging on subsequent plant growth and trace metal content

Summary The uptake of trace metals by two plant species (French bean and maize) has been measured on two soils subjected to various waterlogging regimes. Uptake of both manganese and iron was increased due to soil waterlogging, although reoxidation of the soil affected iron more than manganese. Zinc and copper uptake was influenced by a species factor; French bean (Phaseolus vulgaris) showed preferential uptake of zinc, whereas maize (Zea mays) took up copper preferentially. Uptake of cobalt by both species was increased due to waterlogging, following the pattern of manganese.The abilities of these species to take up trace metals from soil followed the pattern predicted by selective extraction of soil for manganese, iron and cobalt, but not for zinc and copper.     

Comparisons of nine heavy metals in salt gland and liver of greater scaup (Aythya marila), black duck (Anas rubripes) and mallard (A. platyrhynchos)

Levels of nine heavy metals were measured in the livers and salt glands of greater scaup (Aythya marila), black duck (Anas rubripes) and mallard (A. platyrhynchos) from Raritan Bay, New Jersey to determine if the functioning avian salt gland concentrates heavy metals. Heavy metals examined were cadmium, cobalt, chromium, copper, lead, mercury, manganese, nickel and zinc. Heavy metal levels varied significantly by species and tissue for chromium, copper, lead, and manganese, and by tissue for cobalt, mercury, nickel and zinc. In comparing tissues cobalt was higher in the salt glands than in livers of all three species; chromium and nickel were higher in the salt gland than liver for mallard and black duck; and lead, manganese and zinc were higher in the liver than the salt gland in greater scaup. Generally metal levels were higher in the salt gland for mallard and black duck, and in the liver for greater scaup.     

The Correlation of Serum Trace Elements and Heavy Metals with Carotid Artery Atherosclerosis in Maintenance Hemodialysis Patients

Changes in essential trace elements and heavy metals may affect the atherosclerotic state of patients on maintenance hemodialysis (HD). The aim of the study was to evaluate the relation between the serum levels of some trace elements and heavy metals (iron, zinc, manganese, copper, magnesium, cobalt, cadmium, lead, and copper/zinc ratio) and carotid artery intima-media thickness (CIMT) in HD patients. Fifty chronic HD patients without known atherosclerotic disease and 48 age- and sex-matched healthy individuals were included in the study. The serum levels of trace elements (iron, zinc, manganese, copper, and magnesium) and heavy metals (cobalt, cadmium, and lead) were measured by Atomic Adsorption Spectrophotometer (UNICAM-929). CIMT was assessed by carotid artery ultrasonography. The serum levels of iron, zinc, and manganese were lower; levels of copper, magnesium, cobalt, cadmium, lead, and copper/zinc ratio were higher in HD patients compared to controls. CIMT in HD patients were higher than the control group (0.64?±?0.11 vs 0.42?±?0.05, p?相似文献   

Trace element budget in an African savannah ecosystem

The concentration of selected trace elements (Co, Cu, Fe, Mn, Mo, Se, and Zn) were analysed in soils, grass, bush, and tree samples from the Mole National Park, Ghana. The distribution of the essential nutrients: cobalt, copper, manganese, and selenium is controlled by bedrock geology, whereas iron, molybdenum, and zinc distribution is controlled by soil and hydrological processes. In the soils, iron, manganese, and cobalt are largely fixed in the mineral fraction while most of the copper, molybdenum, and selenium in the soils can be extracted by disodium ethylenediaminetetracetate. Copper, cobalt, and manganese appear to be preferentially concentrated in grass species while molybdenum and selenium are concentrated in browse plants. Variations in uptake exist between wet and dry seasons with all trace elements studied, except iron and manganese, showing a marked increased availability in the wet season and increased concentration in the residual fraction of the mineral and organic soils in the dry season. In the dry season the plant concentration of molybdenum and selenium decreased while copper and zine showed increased concentrations and this may be related to a lower pH of the groundwaters at this time. A budget of metal input and output in the ecosystem at Mole has been computed. From this potential dietary deficiencies in cobalt can be observed, however for other metals soil and plant concentrations are sufficient to prevent straightforward deficiencies while the concentrations of molybdenum and selenium are sufficiently low to be considered safe.     

Some essential nutrient elements in forest plants as related to species,plant part,season and location

Summary Natural vegetation common to the forested areas of Nova Scotia was sampled and analyzed for the nutrient elements calcium, phosphorus, cobalt, molybdenum, manganese, zinc and copper. Results showed considerable variation within and between species. Variations due to plant part, season and location of sampling also occurred but to a lesser degree. With the possible exception of molybdenum and copper, the elements are present in adequate quantities for normal nutrition. The average range of nutrient content for cobalt was found to be 0.09 to 0.44; molybdenum 0.01 to 0.32; zinc 13.0 to 91.0; manganese 44 to 594; copper 4.9 to 31.7 in terms of parts per million while calcium ranged from 0.36 to 1.11 per cent and phosphorus from 0.06 to 0.21 per cent. re]19750210     

Levels of trace elements in the liver and diet of free-living koalas, Phascolarctos cinereus (Goldfuss)

The mean liver concentrations of copper, manganese, zinc and cobalt were 0.25, 0.20, 2.97 mmol/kg and 2.81 mumol/kg respectively in free-living koalas in Victoria, Australia. The mean plasma copper concentration was 9.2 mmol/liter which was somewhat below the level in other hindgut fermenters. The mean concentrations of copper, manganese and zinc in their diet (Eucalyptus spp.) were 0.08, 4.46 and 0.27 mmol/kg respectively. Analysis of the data established a significant correlation between the age of the koalas and the inverse of the concentration of the copper (r = -0.67, P less than 0.001) in the liver. There were no such correlations apparent for manganese, zinc or cobalt. The concentrations of trace metals in the Eucalyptus diet for the koala were comparable to those recommended in the diets for other hindgut fermenters such as horse, rabbits and rats. However there was evidence for suboptimal plasma copper levels (mean 9.2 mmol/liter) in some koalas, and reduced liver copper levels in older koalas. Liver histology revealed the presence of brown intracytoplasmic granules in hepatocytes. The size and number of these granules per cell was noted to increase with increasing age of the koala but the chemical nature and the role of the granules was not determined by the histochemical techniques used.     

Seminal plasma trace metal levels in industrial workers

This study compares the seminal plasma trace metal levels of hospital workers with groups of industrial workers in a petroleum refinery, smelter, and chemical plant. The metals measured were the essential metals (copper, zinc, nickel, cobalt, and manganese) and the toxic metals (lead, cadmium, and aluminum). The group mean±SE metal level for each group (50 subjects per group) was calculated, and the statistical significance of the group mean differences of the industrial groups with the hospital group (control) was determined by the Student’s t-test. The differences observed in the smelter group were increased copper and zinc (p≤0.001) and decreased nickel, cobalt, and manganese (p≤0.001,≤0.01). The refinery group differences were increased copper, zinc, and nickel (p≤0.001) but decreased cobalt and manganese (p≤0.001). The chemical group differences were increased zinc (p≤0.001) and decreased cobalt (p≤0.001). The seminal plasma levels of the toxic metals lead and aluminum were increased in each of the industrial groups (p≤0.001). Concurrent differences were (1) decreased accumulation of nickel, cobalt, and manganese in the smelter group, (2) decreased cobalt and managanese in the refinery group, and (3) only decreased cobalt in the chemical group.     

Immunochemistry of sperm-whale myoglobins prepared with various modified porphyrins and metalloporphyrins

1. The preparation and characterization of manganese, iron, cobalt, nickel, copper and zinc metalloporphyrins is described. Ferrihaem was also esterified with pyrid-4-ylpropanol and the derivative characterized as the diester. 2. Complexes of these various porphyrins, as well as protoporphyrin IX, with apomyoglobin were formed and the resulting artificial myoglobins characterized. 3. Very little complex-formation was obtained with nickel, cobalt and manganese metalloporphyrins and apomyoglobin. 4. Myoglobin prepared with copper metalloporphyrin was immunochemically identical with native ferrimyoglobin. All the other artificial myoglobins were less reactive to varying degrees. 5. The changes in antigenic reactivities were attributed to conformational reorganization caused by the different co-ordination tendencies of the various metals or by the modification of the side chains of the haem.     

Manganese in biogenic magnetite crystals from magnetotactic bacteria

Magnetotactic bacteria produce either magnetite (Fe₃O₄) or greigite (Fe₃S₄) crystals in cytoplasmic organelles called magnetosomes. Whereas greigite magnetosomes can contain up to 10 atom% copper, magnetite produced by magnetotactic bacteria was considered chemically pure for a long time and this characteristic was used to distinguish between biogenic and abiogenic crystals. Recently, it was shown that magnetosomes containing cobalt could be produced by three strains of Magnetospirillum . Here we show that magnetite crystals produced by uncultured magnetotactic bacteria can incorporate manganese up to 2.8 atom% of the total metal content (Fe+Mn) when manganese chloride is added to microcosms. Thus, chemical purity can no longer be taken as a strict prerequisite to consider magnetite crystals to be of biogenic origin.     

TRACE-ELEMENT TOXICITIES IN OAT PLANTS

Excessive amounts of nickel, cobalt, chromium, copper, zinc, manganese, molybdenum and aluminium in nutrient solutions supplied to oat plants in sand culture produce ( a ) chlorosis and ( b ) other symptoms specific to the element involved. The specific symptoms are distinct for each metal, although those of cobalt and nickel might be confused.
The toxic effects of nickel, cobalt, copper, zinc, manganese and molybdenum are associated with high concentrations of the element in the leaf tissue, but this is not always so with chromium and aluminium.
The toxic effects of nickel, chromium, copper and molybdenum are associated with a reduced nitrogen content of the plant. Nickel, cobalt, chromium, zinc and manganese increase the concentration of phosphorus in the tissue whilst aluminium decreases it, probably to a deficiency level.
Aluminium reduces the intensity of toxic symptoms produced by nickel—probably by reducing the uptake of nickel and phosphorus. Copper effectively reduces the leaf necrosis produced by nickel, but not the nickel content of the leaf tissue; it is suggested that one factor in nickel toxicity may be inhibition of one or more functions of copper. The other elements slightly increase chlorosis and some increase necrosis.
The order of activitjl of the elements in producing chlorosis is found to be Ni>Cu>Co>Cr>Zn>Mo>Mn. This order, which is related to that giving yield reduction and is similar to the order of stability of metal complexes, is discussed in relation to induced iron deficiency.     

Effect of metal ions on aflatoxin production byAspergillus parasiticus

The effect of iron, copper, cobalt, cadmium, zinc, molybdenum, magnesium and manganese salts was studied on aflatoxin production in relation to mycelial mass. Iron, copper and cadmium salts decreased the aflatoxin production to different levels but a mixed trend was observed depending on salt concentration, with molybdenum, magnesium and manganese. Cobalt and zinc salts stimulated aflatoxin production at all concentrations studied. The maximum increase in aflatoxin production, 655% and 519% was observed in the presence of zinc sulfate and sodium molybdate, respectively. A negative correlation was observed between aflatoxin production and vegetative growth of fungus.     

Effect of cobalt and copper o-phenanthroline complexes on electron transport and energy coupling activity in reaction centers and chromatophores of purple bacteria

The effects of cobalt and copper o-phenanthroline complexes on electron transfer and energy coupling activity in the reaction center and chromatophore preparations of purple bacteria were studied. In terms of their effects on the systems under study these complexes fall into two groups, i.e. cobalt complexes with a high electron transfer activity, which stimulate membrane energization, and copper complexes which contribute to the chromatophore membrane deenergization. Among a variety of complexes studied the perchlorate tris-o-phenanthroline complex Co(II) and the chloride 4,7-diphenyl-o-phenanthroline complex Cu(II) were found to have the highest activity. Both cobalt and copper o-phenanthroline complexes may be a promising tool for regulating bioenergetic processes.     

Determination of trace elements in some natural drugs by atomic absorption spectrometry

Fifteen kinds of common plants, animals, and minerals used as traditional medicines by the Chinese people have been subjected to analysis by atomic absorption spectrometry for its content of seven metals: lead, cadmium, arsenic, mercury, copper, cobalt, and manganese. The concentrations of these elements are significantly different according to their vegetal, animal, or mineral origin. The average values found for lead, cadmium, arsenic, cobalt, and manganese in drugs of mineral origin are higher than those derived from plants and animals, except for copper, which was higher in drugs of animal origin. Our results suggest that the user of traditional Chinese crude drugs should be warned of the potential danger of heavy-metal poisoning because their concentrations seem to be higher than the maximum values allowed by health agencies in several countries.     

Zn,Cu and Co in cyanobacteria: selective control of metal availability

Homeostatic systems for essential and non-essential metals create the cellular environments in which the correct metals are acquired by metalloproteins while the incorrect ones are somehow avoided. Cyanobacteria have metal requirements often absent from other bacteria; copper in thylakoidal plastocyanin, zinc in carboxysomal carbonic anhydrase, cobalt in cobalamin but magnesium in chlorophyll, molybdenum in heterocystous nitrogenase, manganese in thylakoidal water-splitting oxygen-evolving complex. This article reviews: an intracellular trafficking pathway for inward copper supply, the sequestration of surplus zinc by metallothionein (also present in other bacteria) and the detection and export of excess cobalt. We consider the influence of homeostatic proteins on selective metal availability.     

Trace element levels in the liver of rats with acute and chronic fascioliasis and after treatment with zinc-copper hydroxochloride mixed crystals

Quantities of trace elements including copper, zinc, cobalt, manganese and iron were investigated in the liver tissue of rats at the acute or chronic stages of fascioliasis following treatment with zinc-copper hydroxochloride mixed crystals. Oral dosing (with food) of zinc-copper mixed crystals to healthy rats increased zinc and copper levels in the liver and decreased the iron content compared with controls. Manganese and cobalt levels did not change significantly. Significant reductions in all trace elements except manganese occurred in the liver of rats with acute or chronic fascioliasis. Manganese levels were slightly increased in rats at the acute stage and slightly decreased in rats at the chronic stage of fascioliasis. The application of mixed zinc-copper crystals at the acute or chronic stages of fascioliasis lead to a restoration of zinc and copper levels and a slight reduction in the iron levels in liver tissue of rats. The beneficial effects of applied salts were more apparent in rats chronically infected with Fasciola hepatica.     

TOXIC EFFECTS OF HEAVY METALS ON CROP PLANTS GROWN IN SOIL CULTURE

A number of pot experiments were made on various crops, to study the effects on the chemical composition of the plants of treating the soil with cobalt, copper or nickel sulphates, or with the cupric or ferric salts of ethylenediamine-tetraacetic acid (EDTA).
The tops of copper sulphate-treated plants usually contained higher concentrations of copper, manganese, calcium and magnesium and lower concentrations of potassium and phosphorus, than those of control plants.
The most marked effect of cobalt on oat was to increase the concentration of Ca+Mg in the tops; nickel had a profound effect on the composition of barley tops.
Total iron concentration was reduced in oat by cobalt and copper, and in barley by copper and nickel application. Symptoms of iron deficiency appeared only in the oat plants, their incidence being correlated with the reduction in iron concentration in the tops.
The ferric salt of EDTA tended to reduce the incidence of iron deficiency symptoms produced in oat by copper sulphate, but also gave rise to distinct toxic effects. Plants given the cupric salt of EDTA contained unusually large amounts of iron.     

The essential role of cobalt in the inhibition of the cytosolic isozyme of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Nicotiana silvestris by glyphosate

The prime molecular target of glyphosate (N-[phosphonomethyl]glycine), a potent herbicidal and antimicrobial agent, is known to be the shikimate-pathway enzyme, 5-enol-pyruvylshikimate-3-phosphate synthase. Inhibition by glyphosate of an earlier pathway enzyme that is located in the cytosol of higher plants, 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DS-Co), has raised the possibility of dual enzyme targets in vivo. With the recent appreciation that magnesium (and manganese) can replace cobalt as the divalent-metal activator of DS-Co, it has now been possible to show that sensitivity of DS-Co to inhibition by glyphosate is obligately dependent upon the presence of cobalt. Evidence for a cobalt(II):glyphosate complex with octahedral coordination was obtained through examination of the effect of glyphosate upon the visible electronic spectrum of aqueous solutions of cobalt(II) chloride. The presence of glyphosate increased the concentration of cobalt(II) chloride required for enzyme activity, and the concentration of cobalt(II) chloride markedly affected the concentration of glyphosate required for inhibition of DS-Co activity. The extent to which DS-Co is vulnerable to inhibition by glyphosate in vivo depends, therefore, upon the unknown extent to which DS-Co molecules in the cytosol might be associated with cobalt.