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1.
Analysis of FtsZ Assembly by Light Scattering and Determination of the Role of Divalent Metal Cations 总被引:11,自引:5,他引:6
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FtsZ is an ancestral homologue of tubulin that polymerizes in a GTP-dependent manner. In this study, we used 90° angle light scattering to investigate FtsZ polymerization. The critical concentration for polymerization obtained by this method is similar to that obtained by centrifugation, confirming that the light scattering is proportional to polymer mass. Furthermore, the dynamics of FtsZ polymerization could be readily monitored by light scattering. Polymerization was very rapid, reaching steady state within 30 s. The length of the steady-state phase was proportional to the GTP concentration and was followed by a rapid decrease in light scattering. This decrease indicated net depolymerization that always occurred as the GTP in the reaction was consumed. FtsZ polymerization was observed over the pH range 6.5 to 7.9. Importantly, Mg2+ was not required for polymerization although it was required for the dynamic behavior of the polymers. It was reported that 7 to 25 mM Ca2+ mediated dynamic assembly of FtsZ (X.-C. Yu and W. Margolin, EMBO J. 16:5455–5463, 1997). However, we found that Ca2+ was not required for FtsZ assembly and that this concentration of Ca2+ reduced the dynamic behavior of FtsZ assembly. 相似文献
2.
Daniel P. Haeusser Marina Hoashi Anna Weaver Nathan Brown James Pan James A. Sawitzke Lynn C. Thomason Donald L. Court William Margolin 《PLoS genetics》2014,10(3)
Assembly of the essential, tubulin-like FtsZ protein into a ring-shaped structure at the nascent division site determines the timing and position of cytokinesis in most bacteria and serves as a scaffold for recruitment of the cell division machinery. Here we report that expression of bacteriophage λ kil, either from a resident phage or from a plasmid, induces filamentation of Escherichia coli cells by rapid inhibition of FtsZ ring formation. Mutant alleles of ftsZ resistant to the Kil protein map to the FtsZ polymer subunit interface, stabilize FtsZ ring assembly, and confer increased resistance to endogenous FtsZ inhibitors, consistent with Kil inhibiting FtsZ assembly. Cells with the normally essential cell division gene zipA deleted (in a modified background) display normal FtsZ rings after kil expression, suggesting that ZipA is required for Kil-mediated inhibition of FtsZ rings in vivo. In support of this model, point mutations in the C-terminal FtsZ-interaction domain of ZipA abrogate Kil activity without discernibly altering FtsZ-ZipA interactions. An affinity-tagged-Kil derivative interacts with both FtsZ and ZipA, and inhibits sedimentation of FtsZ filament bundles in vitro. Together, these data inspire a model in which Kil interacts with FtsZ and ZipA in the cell to prevent FtsZ assembly into a coherent, division-competent ring structure. Phage growth assays show that kil+ phage lyse ∼30% later than kil mutant phage, suggesting that Kil delays lysis, perhaps via its interaction with FtsZ and ZipA. 相似文献
3.
Santra MK Beuria TK Banerjee A Panda D 《The Journal of biological chemistry》2004,279(25):25959-25965
The assembly of FtsZ plays a major role in bacterial cell division, and it is thought that the assembly dynamics of FtsZ is a finely regulated process. Here, we show that ruthenium red is able to modulate FtsZ assembly in vitro. In contrast to the inhibitory effects of ruthenium red on microtubule polymerization, we found that a substoichiometric concentration of ruthenium red strongly increased the light-scattering signal of FtsZ assembly. Further, sedimentable polymer mass was increased by 1.5- and 2-fold in the presence of 2 and 10 microm ruthenium red, respectively. In addition, ruthenium red strongly reduced the GTPase activity and prevented dilution-induced disassembly of FtsZ polymers. Electron microscopic analysis showed that 4-10 microm of ruthenium red produced thick bundles of FtsZ polymers. The significant increase in the light-scattering signal and pelletable polymer mass in the presence of ruthenium red seemed to be due to the bundling of FtsZ protofilaments into larger polymers rather than the actual increase in the level of polymeric FtsZ. Furthermore, ruthenium red was found to copolymerize with FtsZ, and the copolymerization of substoichiometric amounts of ruthenium red with FtsZ polymers promoted cooperative assembly of FtsZ that produced large bundles. Calcium inhibited the binding of ruthenium red to FtsZ. However, a concentration of calcium 1000-fold higher than that of ruthenium red was required to produce similar effects on FtsZ assembly. Ruthenium red strongly modulated FtsZ polymerization, suggesting the presence of an important regulatory site on FtsZ and suggesting that a natural ligand, which mimics the action of ruthenium red, may regulate the assembly of FtsZ in bacteria. 相似文献
4.
During Escherichia coli cell division, an intracellular complex of cell division proteins known as the Z-ring assembles at midcell during early division and serves as the site of constriction. While the predominant protein in the Z-ring is the widely conserved tubulin homolog FtsZ, the actin homolog FtsA tethers the Z-ring scaffold to the cytoplasmic membrane by binding to FtsZ. While FtsZ is known to function as a dynamic, polymerized GTPase, the assembly state of its partner, FtsA, and the role of ATP are still unclear. We report that a substitution mutation in the FtsA ATP-binding site impairs ATP hydrolysis, phospholipid vesicle remodeling in vitro, and Z-ring assembly in vivo. We demonstrate by transmission electron microscopy and Förster Resonance Energy Transfer that a truncated FtsA variant, FtsA(ΔMTS) lacking a C-terminal membrane targeting sequence, self assembles into ATP-dependent filaments. These filaments coassemble with FtsZ polymers but are destabilized by unassembled FtsZ. These findings suggest a model wherein ATP binding drives FtsA polymerization and membrane remodeling at the lipid surface, and FtsA polymerization is coregulated with FtsZ polymerization. We conclude that the coordinated assembly of FtsZ and FtsA polymers may serve as a key checkpoint in division that triggers cell wall synthesis and division progression. 相似文献
5.
Assembly of the tubulin-like protein FtsZ at or near the cytoplasmic membrane is one of the earliest steps in division of bacteria such as Escherichia coli. Exactly what constitutes the site at which FtsZ acts is less clear. To investigate the influence of the membrane phospholipids on FtsZ localization and assembly, we have elaborated with the Langmuir technique a two-lipid monolayer made of dilauryl-phosphatidylethanolamine (DLPE) and dipalmitoyl-phosphatidylglycerol (DPPG). This monolayer comprised stable condensed domains in an expanded continuous phase. In the presence of GTP, FtsZ assembly disrupts the condensed domains within 5 min. After several hours, with or without GTP, FtsZ assembled into large aggregates at the domain interface. We suggest that the GTP-induced polymerization of FtsZ is coupled to the association of FtsZ protofilaments with domain interfaces. 相似文献
6.
Richa Jaiswal Ronak Y. Patel Jayant Asthana Bhavya Jindal Petety V. Balaji Dulal Panda 《The Journal of biological chemistry》2010,285(41):31796-31805
Recently, we found that divalent calcium has no detectable effect on the assembly of Mycobacterium tuberculosis FtsZ (MtbFtsZ), whereas it strongly promoted the assembly of Escherichia coli FtsZ (EcFtsZ). While looking for potential calcium binding residues in EcFtsZ, we found a mutation (E93R) that strongly promoted the assembly of EcFtsZ. The mutation increased the stability and bundling of the FtsZ protofilaments and produced a dominating effect on the assembly of the wild type FtsZ (WT-FtsZ). Although E93R-FtsZ was found to bind to GTP similarly to the WT-FtsZ, it displayed lower GTPase activity than the WT-FtsZ. E93R-FtsZ complemented for its wild type counterpart as observed by a complementation test using JKD7–1/pKD3 cells. However, the bacterial cells became elongated upon overexpression of the mutant allele. We modeled the structure of E93R-FtsZ using the structures of MtbFtsZ/Methanococcus jannaschi FtsZ (MjFtsZ) dimers as templates. The MtbFtsZ-based structure suggests that the Arg93-Glu138 salt bridge provides the additional stability, whereas the effect of mutation appears to be indirect (allosteric) if the EcFtsZ dimer is similar to that of MjFtsZ. The data presented in this study suggest that an increase in the stability of the FtsZ protofilaments is detrimental for the bacterial cytokinesis. 相似文献
7.
Makoto T. Fujiwara Yasushi Yoshioka Tomonari Hirano Yusuke Kazama Tomoko Abe Kensuke Hayashi Ryuuichi D. Itoh 《Plant signaling & behavior》2012,7(1):34-37
Organelle dynamics in the plant male gametophyte has received attention for its importance in pollen tube growth and cytoplasmic inheritance. We recently revealed the dynamic behaviors of plastids in living Arabidopsis pollen grains and tubes, using an inherent promoter-driven FtsZ1–green fluorescent protein (GFP) fusion. Here, we further monitored the movement of pollen tube plastids with an actin1 promoter-driven, stroma-targeted yellow fluorescent protein (YFP). In elongating pollen tubes, most plastids localized to the tube shank, where they displayed either retarded and unsteady motion, or fast, directional, and long-distance movement along the tube polarity. Efficient plastid tracking further revealed a population of tip-forwarding plastids that undergo a fluctuating motion(s) before traveling backward. The behavior of YFP-labeled plastids in pollen basically resembled that of FtsZ1–GFP-labeled plastids, thus validating the use of FtsZ1–GFP for simultaneous visualization of the stroma and the plastid-dividing FtsZ ring. 相似文献
8.
Assembly of FtsZ was completely inhibited by low concentrations of urea and its unfolding occurred in two steps in the presence of urea, with the formation of an intermediate [Santra MK & Panda D (2003) J Biol Chem278, 21336-21343]. In this study, using the fluorescence of 1-anilininonaphthalene-8-sulfonic acid and far-UV circular dichroism spectroscopy, we found that a natural osmolyte, trimethylamine N-oxide (TMAO), counteracted the denaturing effects of urea and guanidium chloride on FtsZ. TMAO also protected assembly and bundling of FtsZ protofilaments from the denaturing effects of urea and guanidium chloride. Furthermore, the standard free energy changes for unfolding of FtsZ were estimated to be 22.5 and 28.4 kJ.mol(-1) in the absence and presence of 0.6 M TMAO, respectively. The data are consistent with the view that osmolytes counteract denaturant-induced unfolding of proteins by destabilizing the unfolded states. Interestingly, TMAO was also found to affect the assembly properties of native FtsZ. TMAO increased the light-scattering signal of the FtsZ assembly, increased sedimentable polymer mass, enhanced bundling of FtsZ protofilaments and reduced the GTPase activity of FtsZ. Similar to TMAO, monosodium glutamate, a physiological osmolyte in bacteria, which induces assembly and bundling of FtsZ filaments in vitro[Beuria TK, Krishnakumar SS, Sahar S, Singh N, Gupta K, Meshram M & Panda D (2003) J Biol Chem278, 3735-3741], was also found to counteract the deleterious effects of urea on FtsZ. The results together suggested that physiological osmolytes may regulate assembly and bundling of FtsZ in bacteria and that they may protect the functionality of FtsZ under environmental stress conditions. 相似文献
9.
Katie J. Porter Lingyan Cao Katherine W. Osteryoung 《The Journal of biological chemistry》2023,299(3)
The division of cyanobacteria and their chloroplast descendants is orchestrated by filamenting temperature-sensitive Z (FtsZ), a cytoskeletal GTPase that polymerizes into protofilaments that form a “Z ring” at the division site. The Z ring has both a scaffolding function for division-complex assembly and a GTPase-dependent contractile function that drives cell or organelle constriction. A single FtsZ performs these functions in bacteria, whereas in chloroplasts, they are performed by two copolymerizing FtsZs, called AtFtsZ2 and AtFtsZ1 in Arabidopsis thaliana, which promote protofilament stability and dynamics, respectively. To probe the differences between cyanobacterial and chloroplast FtsZs, we used light scattering to characterize the in vitro protofilament dynamics of FtsZ from the cyanobacterium Synechococcus elongatus PCC 7942 (SeFtsZ) and investigate how coassembly of AtFtsZ2 or AtFtsZ1 with SeFtsZ influences overall dynamics. SeFtsZ protofilaments assembled rapidly and began disassembling before GTP depletion, whereas AtFtsZ2 protofilaments were far more stable, persisting beyond GTP depletion. Coassembled SeFtsZ–AtFtsZ2 protofilaments began disassembling before GTP depletion, similar to SeFtsZ. In contrast, AtFtsZ1 did not alter disassembly onset when coassembled with SeFtsZ, but fluorescence recovery after photobleaching analysis showed it increased the turnover of SeFtsZ subunits from SeFtsZ–AtFtsZ1 protofilaments, mirroring its effect upon coassembly with AtFtsZ2. Comparisons of our findings with previous work revealed consistent differences between cyanobacterial and chloroplast FtsZ dynamics and suggest that the scaffolding and dynamics-promoting functions were partially separated during evolution of two chloroplast FtsZs from their cyanobacterial predecessor. They also suggest that chloroplasts may have evolved a mechanism distinct from that in cyanobacteria for promoting FtsZ protofilament dynamics. 相似文献
10.
Chen Y Anderson DE Rajagopalan M Erickson HP 《The Journal of biological chemistry》2007,282(38):27736-27743
We have investigated the assembly of FtsZ from Mycobacterium tuberculosis (MtbFtsZ). Electron microscopy confirmed the previous observation that MtbFtsZ assembled into long, two-stranded filaments at pH 6.5. However, we found that assembly at pH 7.2 or 7.7 produced predominantly short, one-stranded protofilaments, similar to those of Escherichia coli FtsZ (EcFtsZ). Near pH 7, which is close to the pH of M. tuberculosis cytoplasm, MtbFtsZ formed a mixture of single- and two-stranded filaments. We developed a fluorescence resonance energy transfer assay to measure the kinetics of initial assembly and the dynamic properties at steady state. Assembly of MtbFtsZ reached a plateau after 60-100 s, about 10 times slower than EcFtsZ. The initial assembly kinetics were similar at pH 6.5 and 7.7, despite the striking difference in the polymer structures. Both were fit with a cooperative assembly mechanism involving a weak dimer nucleus, similar to EcFtsZ but with slower kinetics. Subunit turnover and GTPase at steady state were also about 10 times slower for MtbFtsZ than for EcFtsZ. Specifically, the half-time for subunit turnover in vitro at pH 7.7 was 42 s for MtbFtsZ compared with 5.5 s for EcFtsZ. Photobleaching studies in vivo showed a range of turnover half-times with an average of 25 s for MtbFtsZ as compared with 9 s for EcFtsZ. 相似文献
11.
Sergey Ivanchenko William J. Godinez Marko Lampe Hans-Georg Kr?usslich Roland Eils Karl Rohr Christoph Br?uchle Barbara Müller Don C. Lamb 《PLoS pathogens》2009,5(11)
Assembly and release of human immunodeficiency virus (HIV) occur at the plasma membrane of infected cells and are driven by the Gag polyprotein. Previous studies analyzed viral morphogenesis using biochemical methods and static images, while dynamic and kinetic information has been lacking until very recently. Using a combination of wide-field and total internal reflection fluorescence microscopy, we have investigated the assembly and release of fluorescently labeled HIV-1 at the plasma membrane of living cells with high time resolution. Gag assembled into discrete clusters corresponding to single virions. Formation of multiple particles from the same site was rarely observed. Using a photoconvertible fluorescent protein fused to Gag, we determined that assembly was nucleated preferentially by Gag molecules that had recently attached to the plasma membrane or arrived directly from the cytosol. Both membrane-bound and cytosol derived Gag polyproteins contributed to the growing bud. After their initial appearance, assembly sites accumulated at the plasma membrane of individual cells over 1–2 hours. Assembly kinetics were rapid: the number of Gag molecules at a budding site increased, following a saturating exponential with a rate constant of ∼5×10−3 s−1, corresponding to 8–9 min for 90% completion of assembly for a single virion. Release of extracellular particles was observed at ∼1,500±700 s after the onset of assembly. The ability of the virus to recruit components of the cellular ESCRT machinery or to undergo proteolytic maturation, or the absence of Vpu did not significantly alter the assembly kinetics. 相似文献
12.
13.
The division inhibitor EzrA contains a seven-residue patch required for maintaining the dynamic nature of the medial FtsZ ring
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The essential cytoskeletal protein FtsZ assembles into a ring-like structure at the nascent division site and serves as a scaffold for the assembly of the prokaryotic division machinery. We previously characterized EzrA as an inhibitor of FtsZ assembly in Bacillus subtilis. EzrA interacts directly with FtsZ to prevent aberrant FtsZ assembly and cytokinesis at cell poles. EzrA also concentrates at the cytokinetic ring in an FtsZ-dependent manner, although its precise role at this position is not known. Here, we identified a conserved patch of amino acids in the EzrA C terminus that is essential for localization to the FtsZ ring. Mutations in this patch (designated the “QNR patch”) abolish EzrA localization to midcell but do not significantly affect EzrA's ability to inhibit FtsZ assembly at cell poles. ezrA QNR patch mutant cells exhibit stabilized FtsZ assembly at midcell and are significantly longer than wild-type cells, despite lacking extra FtsZ rings. These results indicate that EzrA has two distinct activities in vivo: (i) preventing aberrant FtsZ ring formation at cell poles through inhibition of de novo FtsZ assembly and (ii) maintaining proper FtsZ assembly dynamics within the medial FtsZ ring, thereby rendering it sensitive to the factors responsible for coordinating cell growth and cell division. 相似文献
14.
Pankaj B. Tiwari Radhakrishnan Mahalakshmi 《Protein science : a publication of the Protein Society》2021,30(3):624
The outer membrane of a Gram‐negative bacterium is a crucial barrier between the external environment and its internal physiology. This barrier is bridged selectively by β‐barrel outer membrane proteins (OMPs). The in vivo folding and biogenesis of OMPs necessitates the assistance of the outer membrane chaperone BamA. Nevertheless, OMPs retain the ability of independent self‐assembly in vitro. Hence, it is unclear whether substrate–chaperone dynamics is influenced by the intrinsic ability of OMPs to fold, the magnitude of BamA–OMP interdependence, and the contribution of BamA to the kinetics of OMP assembly. We addressed this by monitoring the assembly kinetics of multiple 8‐stranded β‐barrel OMP substrates with(out) BamA. We also examined whether BamA is species‐specific, or nonspecifically accelerates folding kinetics of substrates from independent species. Our findings reveal BamA as a substrate‐independent promiscuous molecular chaperone, which assists the unfolded OMP to overcome the kinetic barrier imposed by the bilayer membrane. We additionally show that while BamA kinetically accelerates OMP folding, the OMP primary sequence remains a vital deciding element in its assembly rate. Our study provides unexpected insights on OMP assembly and the functional relevance of BamA in vivo. 相似文献
15.
Maria A. Schumacher Wenjie Zeng Kuo-Hsiang Huang Lukasz Tchorzewski Anuradha Janakiraman 《The Journal of biological chemistry》2016,291(5):2485-2498
In Escherichia coli cell division is driven by the tubulin-like GTPase, FtsZ, which forms the cytokinetic Z-ring. The Z-ring serves as a dynamic platform for the assembly of the multiprotein divisome, which catalyzes membrane cleavage to create equal daughter cells. Several proteins effect FtsZ assembly, thereby providing spatiotemporal control over cell division. One important class of FtsZ interacting/regulatory proteins is the Z-ring-associated proteins, Zaps, which typically modulate Z-ring formation by increasing lateral interactions between FtsZ protofilaments. Strikingly, these Zap proteins show no discernable sequence similarity, suggesting that they likely harbor distinct structures and mechanisms. The 19.8-kDa ZapC in particular shows no homology to any known protein. To gain insight into ZapC function, we determined its structure to 2.15 Å and performed genetic and biochemical studies. ZapC is a monomer composed of two domains, an N-terminal α/β region and a C-terminal twisted β barrel-like domain. The structure contains two pockets, one on each domain. The N-domain pocket is lined with residues previously implicated to be important for ZapC function as an FtsZ bundler. The adjacent C-domain pocket contains a hydrophobic center surrounded by conserved basic residues. Mutagenesis analyses indicate that this pocket is critical for FtsZ binding. An extensive FtsZ binding surface is consistent with the fact that, unlike many FtsZ regulators, ZapC binds the large FtsZ globular core rather than C-terminal tail, and the presence of two adjacent pockets suggests possible mechanisms for ZapC-mediated FtsZ bundling. 相似文献
16.
Elisa J. Cabré Bego?a Monterroso Carlos Alfonso Alicia Sánchez-Gorostiaga Belén Reija Mercedes Jiménez Miguel Vicente Silvia Zorrilla Germán Rivas 《PloS one》2015,10(5)
Division site selection is achieved in bacteria by different mechanisms, one of them being nucleoid occlusion, which prevents Z-ring assembly nearby the chromosome. Nucleoid occlusion in E. coli is mediated by SlmA, a sequence specific DNA binding protein that antagonizes FtsZ assembly. Here we show that, when bound to its specific target DNA sequences (SBS), SlmA reduces the lifetime of the FtsZ protofilaments in solution and of the FtsZ bundles when located inside permeable giant vesicles. This effect appears to be essentially uncoupled from the GTPase activity of the FtsZ protofilaments, which is insensitive to the presence of SlmA·SBS. The interaction of SlmA·SBS with either FtsZ protofilaments containing GTP or FtsZ oligomers containing GDP results in the disassembly of FtsZ polymers. We propose that SlmA·SBS complexes control the polymerization state of FtsZ by accelerating the disassembly of the FtsZ polymers leading to their fragmentation into shorter species that are still able to hydrolyze GTP at the same rate. SlmA defines therefore a new class of inhibitors of the FtsZ ring different from the SOS response regulator SulA and from the moonlighting enzyme OpgH, inhibitors of the GTPase activity. SlmA also shows differences compared with MinC, the inhibitor of the division site selection Min system, which shortens FtsZ protofilaments by interacting with the GDP form of FtsZ. 相似文献
17.
FtsZ, a bacterial homolog of eukaryotic tubulin, assembles into the Z ring required for cytokinesis. In Escherichia coli, FtsZ interacts directly with FtsA and ZipA, which tether the Z ring to the membrane. We used three-dimensional structured illumination microscopy to compare the localization patterns of FtsZ, FtsA, and ZipA at high resolution in Escherichia coli cells. We found that FtsZ localizes in patches within a ring structure, similar to the pattern observed in other species, and discovered that FtsA and ZipA mostly colocalize in similar patches. Finally, we observed similar punctate and short polymeric structures of FtsZ distributed throughout the cell after Z rings were disassembled, either as a consequence of normal cytokinesis or upon induction of an endogenous cell division inhibitor.The assembly of the bacterial tubulin FtsZ has been well studied in vitro, but the fine structure of the cytokinetic Z ring it forms in vivo is not well defined. Super-resolution microscopy methods including photoactivated localization microscopy (PALM) and three-dimensional-structured illumination microscopy (3D-SIM) have recently provided a more detailed view of Z-ring structures. Two-dimensional PALM showed that Z rings in Escherichia coli are likely composed of loosely-bundled dynamic protofilaments (1,2). Three-dimensional PALM studies of Caulobacter crescentus initially showed that Z rings were comprised of loosely bundled protofilaments forming a continuous but dynamic ring (1–3). However, a more recent high-throughput study showed that the Z rings of this bacterium are patchy or discontinuous (4), similar to Z rings of Bacillus subtilis and Staphylococcus aureus using 3D-SIM (5). Strauss et al. (5) also demonstrated that the patches in B. subtilis Z rings are highly dynamic.Assembly of the Z ring is modulated by several proteins that interact directly with FtsZ and enhance assembly or disassembly (6). For example, FtsA and ZipA promote ring assembly in E. coli by tethering it to the cytoplasmic membrane (7,8). SulA is an inhibitor of FtsZ assembly, induced only after DNA damage, which sequesters monomers of FtsZ to prevent its assembly into a Z ring (9). Our initial goals were to visualize Z rings in E. coli using 3D-SIM, and then examine whether any FtsZ polymeric structures remain after SulA induction. We also asked whether FtsA and ZipA localized in patchy patterns similar to those of FtsZ.We used a DeltaVision OMX V4 Blaze microscope (Applied Precision, GE Healthcare, Issaquah, WA) to view the high-resolution localization patterns of FtsZ in E. coli cells producing FtsZ-GFP (Fig. 1). Three-dimensional views were reconstructed using softWoRx software (Applied Precision). To rule out GFP artifacts, we also visualized native FtsZ from a wild-type strain (WM1074) by immunofluorescence (IF).Open in a separate windowFigure 1Localization of FtsZ in E. coli. (A) Cell with a Z ring labeled with FtsZ-GFP. (B) Rotated view of Z ring in panel A. (C) Cell with a Z ring labeled with DyLight 550 (Thermo Fisher Scientific, Waltham, MA). (D) Rotated view of Z ring in panel C. (B1 and D1) Three-dimensional surface intensity plots of Z rings in panels B and D, respectively. (E) A dividing cell producing FtsZ-GFP. The cell outline is shown in the schematic. (Asterisk) Focus of FtsZ localization; (open dashed ovals) filamentous structures of FtsZ. Three-dimensional surface intensity plots were created using the software ImageJ (19). Scale bars, 1 μm.Both FtsZ-GFP (Fig. 1, A, B, and B1) and IF staining for FtsZ (Fig. 1, C, D, and D1) consistently localized to patches around the ring circumference, similar to the B. subtilis and C. crescentus FtsZ patterns (4,5). Analysis of fluorescence intensities (see Fig. S1, A and B, in the Supporting Material) revealed that the majority of Z rings contain one or more gaps in which intensity decreases to background levels (82% for FtsZ-GFP and 69% for IF). Most rings had 3–5 areas of lower intensity, but only a small percentage of these areas had fluorescence below background intensity (34% for FtsZ-GFP and 21% for IF), indicating that the majority of areas with lower intensity contain at least some FtsZ.To elucidate how FtsZ transitions from a disassembled ring to a new ring, we imaged a few dividing daughter cells before they were able to form new Z rings (Fig. 1
E). Previous conventional microscopy had revealed dynamic FtsZ helical structures (10), but the resolution had been insufficient to see further details. Here, FtsZ visualized in dividing cells by 3D-SIM localized throughout as a mixture of patches and randomly-oriented short filaments (asterisk and dashed oval in Fig. 1, respectively). These structures may represent oligomeric precursors of Z ring assembly.To visualize FtsZ after Z-ring disassembly another way, we overproduced SulA, a protein that blocks FtsZ assembly. We examined E. coli cells producing FtsZ-GFP after induction of sulA expression from a pBAD33-sulA plasmid (pWM1736) with 0.2% arabinose. After 30 min of sulA induction, Z rings remained intact in most cells (Fig. 2
A and data not shown). The proportion of cellular FtsZ-GFP in the ring before and after induction of sulA was consistent with previous data (data not shown) (1,11).Open in a separate windowFigure 2Localization of FtsZ after overproduction of SulA. (A) Cell producing FtsZ-GFP after 0.2% arabinose induction of SulA for 30 min. (B) After 45 min. (B1) Magnified cell shown in panel B. (C) Cell producing native FtsZ labeled with AlexaFluor 488 (Life Technologies, Carlsbad, CA) 30 min after induction; (D) 45 min after induction. (D1) Magnified cell shown in panel D. Scale bars, 1 μm. (Asterisk) Focus of FtsZ localization; (open dashed ovals) filamentous structures of FtsZ.Notably, after 45 min of sulA induction, Z rings were gone (Fig. 2, B and B1), replaced by numerous patches and randomly-oriented short filaments (asterisk and dashed ovals in Fig. 2), similar to those observed in a dividing cell. FtsZ normally rapidly recycles from free monomers to ring-bound polymers (11), but a critical concentration of SulA reduces the pool of available FtsZ monomers, resulting in breakdown of the Z ring (9). The observed FtsZ-GFP patches and filaments are likely FtsZ polymers that disassemble before they can organize into a ring.We confirmed this result by overproducing SulA in wild-type cells and detecting FtsZ localization by IF (Fig. 2, C, D, and D1). The overall fluorescence patterns in cells producing FtsZ-GFP versus cells producing only native FtsZ were similar (Fig. 2, B1 and D1), although we observed fewer filaments with IF, perhaps because FtsZ-GFP confers slight resistance to SulA, or because the increased amount of FtsZ in FtsZ-GFP producing cells might titrate the SulA more effectively.Additionally, we wanted to observe the localization patterns of the membrane tethers FtsA and ZipA. Inasmuch as both proteins bind to the same C-terminal conserved tail of FtsZ (12–14), they would be expected to colocalize with the circumferential FtsZ patches in the Z ring. We visualized FtsA using protein fusions to mCherry and GFP (data not shown) as well as IF using a wild-type strain (WM1074) (Fig. 3
A). We found that the patchy ring pattern of FtsA localization was similar to the FtsZ pattern. ZipA also displayed a similar patchy localization in WM1074 by IF (Fig. 3
B).Open in a separate windowFigure 3Localization of FtsA (A) and ZipA (B) by IF using AlexaFluor 488. (C) FtsA-GFP ring. (D) Same cell shown in panel C with ZipA labeled with DyLight 550. (C1 and D1) Three-dimensional surface intensity plots of FtsA ring from panel C or ZipA ring from panel D, respectively. (E) Merged image of FtsA (green) and ZipA (red) from the ring shown in panels C and D. (F) Intensity plot of FtsA (green) and ZipA (red) of ring shown in panel E. The plot represents intensity across a line drawn counterclockwise from the top of the ring around the circumference, then into its lumen. Red/green intensity plot and three-dimensional surface intensity plots were created using the software ImageJ (19). Scale bar, 1 μm.To determine whether FtsA and ZipA colocalized to these patches, we used a strain producing FtsA-GFP (WM4679) for IF staining of ZipA using a red secondary antibody. FtsA-GFP (Fig. 3
C) and ZipA (Fig. 3
D) had similar patterns of fluorescence, although the three-dimensional intensity profiles (Fig. 3, C1 and D1) reveal slight differences in intensity that are also visible in a merged image (Fig. 3
E). Quantitation of fluorescence intensities around the circumference of the rings revealed that FtsA and ZipA colocalized almost completely in approximately half of the rings analyzed (Fig. 3
F, and see Fig. S2
A), whereas in the other rings there were significant differences in localization in one or more areas (see Fig. S2
B). FtsA and ZipA bind to the same C-terminal peptide of FtsZ and may compete for binding. Cooperative self-assembly of FtsA or ZipA might result in large-scale differential localization visible by 3D-SIM.In conclusion, our 3D-SIM analysis shows that the patchy localization of FtsZ is conserved in E. coli and suggests that it may be widespread among bacteria. After disassembly of the Z ring either in dividing cells or by excess levels of the cell division inhibitor SulA, FtsZ persisted as patches and short filamentous structures. This is consistent with a highly dynamic population of FtsZ monomers and oligomers outside the ring, originally observed as mobile helices in E. coli by conventional fluorescence microscopy (10) and by photoactivation single-molecule tracking (15). FtsA and ZipA, which bind to the same segment of FtsZ and tether it to the cytoplasmic membrane, usually display a similar localization pattern to FtsZ and each other, although in addition to the differences we detect by 3D-SIM, there are also likely differences that are beyond its ∼100-nm resolution limit in the X,Y plane.As proposed previously (16), gaps between FtsZ patches may be needed to accommodate a switch from a sparse Z ring to a more condensed ring, which would provide force to drive ring constriction (17). If this model is correct, the gaps should close upon ring constriction, although this may be beyond the resolution of 3D-SIM in constricted rings. Another role for patches could be to force molecular crowding of low-abundance septum synthesis proteins such as FtsI, which depend on FtsZ/FtsA/ZipA for their recruitment, into a few mobile supercomplexes.How are FtsZ polymers organized within the Z-ring patches? Recent polarized fluorescence data suggest that FtsZ polymers are oriented both axially and circumferentially within the Z ring in E. coli (18). The seemingly random orientation of the non-ring FtsZ polymeric structures we observe here supports the idea that there is no strong constraint requiring FtsZ oligomers to follow a circumferential path around the cell cylinder. The patches of FtsZ in the unperturbed E. coli Z ring likely represent randomly oriented clusters of FtsZ filaments that are associated with ZipA, FtsA, and essential septum synthesis proteins. New super-resolution microscopy methods should continue to shed light on the in vivo organization of these protein assemblies. 相似文献
18.
Víctor M. Hernández-Rocamora Concepción García-Monta?és Belén Reija Bego?a Monterroso William Margolin Carlos Alfonso Silvia Zorrilla Germán Rivas 《The Journal of biological chemistry》2013,288(34):24625-24635
The interaction of MinC with FtsZ and its effects on FtsZ polymerization were studied under close to physiological conditions by a combination of biophysical methods. The Min system is a widely conserved mechanism in bacteria that ensures the correct placement of the division machinery at midcell. MinC is the component of this system that effectively interacts with FtsZ and inhibits the formation of the Z-ring. Here we report that MinC produces a concentration-dependent reduction in the size of GTP-induced FtsZ protofilaments (FtsZ-GTP) as demonstrated by analytical ultracentrifugation, dynamic light scattering, fluorescence correlation spectroscopy, and electron microscopy. Our experiments show that, despite being shorter, FtsZ protofilaments maintain their narrow distribution in size in the presence of MinC. The protein had the same effect regardless of its addition prior to or after FtsZ polymerization. Fluorescence anisotropy measurements indicated that MinC bound to FtsZ-GDP with a moderate affinity (apparent KD ∼10 μm at 100 mm KCl and pH 7.5) very close to the MinC concentration corresponding to the midpoint of the inhibition of FtsZ assembly. Only marginal binding of MinC to FtsZ-GTP protofilaments was observed by analytical ultracentrifugation and fluorescence correlation spectroscopy. Remarkably, MinC effects on FtsZ-GTP protofilaments and binding affinity to FtsZ-GDP were strongly dependent on ionic strength, being severely reduced at 500 mm KCl compared with 100 mm KCl. Our results support a mechanism in which MinC interacts with FtsZ-GDP, resulting in smaller protofilaments of defined size and having the same effect on both preassembled and growing FtsZ protofilaments. 相似文献
19.
A rapid fluorescence assay for FtsZ assembly indicates cooperative assembly with a dimer nucleus
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FtsZ is the major cytoskeletal protein operating in bacterial cell division. FtsZ assembles into protofilaments in vitro, and there has been some controversy over whether the assembly is isodesmic or cooperative. Assembly has been assayed previously by sedimentation and light scattering. However, these techniques will under-report small polymers. We have now produced a mutant of Escherichia coli FtsZ, L68W, which gives a 250% increase in tryptophan fluorescence upon polymerization. This provides a real-time assay of polymer that is directly proportional to the concentration of subunit interfaces. FtsZ-L68W is functional for cell division, and should therefore be a valid model for studying the thermodynamics and kinetics of FtsZ assembly. We assayed assembly at pH 7.7 and pH 6.5, in 2.5 mM EDTA. EDTA blocks GTP hydrolysis and should give an assembly reaction that is not complicated by the irreversible hydrolysis step. Assembly kinetics was determined with a stopped-flow device for a range of FtsZ concentrations. When assembly was initiated by adding 0.2 mM GTP, fluorescence increase showed a lag, followed by nucleation, elongation, and a plateau. The assembly curves were fit to a cooperative mechanism that included a monomer activation step, a weak dimer nucleus, and elongation. Fragmentation was absent in the model, another characteristic of cooperative assembly. We are left with an enigma: how can the FtsZ protofilament, which appears to be one-subunit thick, assemble with apparent cooperativity? 相似文献
20.
The precise spatial and temporal control of bacterial cell division is achieved through the balanced actions of factors that inhibit assembly of the tubulin-like protein FtsZ at aberrant subcellular locations or promote its assembly at the future sites of division. In Bacillus subtilis, the membrane anchored cell division protein EzrA, interacts directly with FtsZ to prevent aberrant FtsZ assembly at cell poles and contributes to the inherently dynamic nature of the cytokinetic ring. Recent work suggests EzrA also serves as a scaffolding protein to coordinate lateral growth with cell wall biosynthesis through interactions with a host of proteins, a finding consistent with EzrA''s four extensive coiled-coil domains. In a previous study we identified a conserved patch of residues near EzrA''s C-terminus (the QNR motif) that are critical for maintenance of a dynamic cytokinetic ring, but dispensable for EzrA-mediated inhibition of FtsZ assembly at cell poles. In an extension of this work, here we report that EzrA''s two C-terminal coiled-coils function in concert with the QNR motif to mediate interactions with FtsZ and maintain the dynamic nature of the cytokinetic ring. In contrast, EzrA''s two N-terminal coiled-coils are dispensable for interaction between EzrA and FtsZ in vitro and in vivo, but required for EzrA mediated inhibition of FtsZ assembly at cell poles. Finally, chimeric analysis indicates that EzrA''s transmembrane anchor plays a generic role: concentrating EzrA at the plasma membrane where presumably it can most effectively modulate FtsZ assembly. 相似文献