Associations between chronotype,morbidity and mortality in the UK Biobank cohort

Later chronotype (i.e. evening preference) and later timing of sleep have been associated with greater morbidity, including higher rates of metabolic dysfunction and cardiovascular disease (CVD). However, no one has examined whether chronotype is associated with mortality risk to date. Our objective was to test the hypothesis that being an evening type is associated with increased mortality in a large cohort study, the UK Biobank. Our analysis included 433 268 adults aged 38–73 at the time of enrolment and an average 6.5-year follow-up. The primary exposure was chronotype, as assessed through a single self-reported question-defining participants as definite morning types, moderate morning types, moderate evening types or definite evening types. The primary outcomes were all-cause mortality and mortality due to CVD. Prevalent disease was also compared among the chronotype groups. Analyses were adjusted for age, sex, ethnicity, smoking, body mass index, sleep duration, socioeconomic status and comorbidities. Greater eveningness, particularly being a definite evening type, was significantly associated with a higher prevalence of all comorbidities. Comparing definite evening type to definite morning type, the associations were strongest for psychological disorders (OR 1.94, 95% CI 1.86–2.02, p = < 0.001), followed by diabetes (OR 1.30, 95% CI 1.24–1.36, p = < 0.001), neurological disorders (OR 1.25, 95% CI 1.20–1.30, p = < 0.001), gastrointestinal/abdominal disorders (OR 1.23, 95% CI 1.19–1.27, p = < 0.001) and respiratory disorders (OR 1.22, 95% CI 1.18–1.26, p = < 0.001). The total number of deaths was 10 534, out of which 2127 were due to CVD. Greater eveningness, based on chronotype as an ordinal variable, was associated with a small increased risk of all-cause mortality (HR 1.02, 95% CI 1.004–1.05, p = 0.017) and CVD mortality (HR 1.04, 95% CI 1.00–1.09, p = 0.06). Compared to definite morning types, definite evening types had significantly increased risk of all-cause mortality (HR 1.10, 95% CI 1.02–1.18, p = 0.012). This first report of increased mortality in evening types is consistent with previous reports of increased levels of cardiometabolic risk factors in this group. Mortality risk in evening types may be due to behavioural, psychological and physiological risk factors, many of which may be attributable to chronic misalignment between internal physiological timing and externally imposed timing of work and social activities. These findings suggest the need for researching possible interventions aimed at either modifying circadian rhythms in individuals or at allowing evening types greater working hour flexibility.     

SHBG,plasma, and urinary androgens in weight lifters after a strength training

Previous studies with different results have suggested that total and bioavailable testosterone levels are modified by physical exercise. Such changes may be related to modifications in cortisol levels and could be reflected in some urine androgens. To determine how weight lifting training may affect serum and urinary androgens, we measured total serum testosterone (T), cortisol, sex hormone binding globulin (SHBG) and urinary testosterone, epitestosterone, androsterone, and etiocholanolone, in a group of 19 elite weight lifters after 20 weeks of training. SHBG increased (from 27.5 ± 9.5 to 34.7 ± 8.1 nM, p < 0.05) whereas T/SHBG decreased significantly (from 1.10 ± 0.4 to 0.85 ± 0.3, p < 0.05). Serum total testosterone and cortisol did not change significantly. In urine, androsterone and etiocholanolone decreased significantly, whereas testosterone and epitestosterone remained unchanged. Changes in T/SHBG were related positively with changes in urinary androgens (r = 0.680, p < 0.01), and changes in SHBG were negatively related with changes in urinary androgens (r = −0.578, p < 0.01). These results suggest that intense physical activity may have an influence on the elimination of androgenic hormones due mainly to changes in their transporting protein SHBG.     

Residual effects of four pesticides on the predatory bug,Orius albidipennis Reut. (Hem.: Anthocoridae)

Residual effects of abamectin (0.02%), propargite (0.1%), dichlorvos (DDVP) (0.15%) and pymetrozine (0.15%) were determined on Orius albidipennis at laboratory conditions. Planted cucumbers were sprayed with the highest recommended concentrations of all insecticides and experimental treatments were monitored during 20 days based on the period of pesticides residues. On the first day after spraying, the greatest of mortality belonged to dichlorvos and pymetrozine (97 ± 1.22, 57 ± 3.1, respectively, p < 0.01), while abamectin and propargite had the highest mortality after four (44 ± 1.87 and 24 ± 2.91, respectively, p < 0.01) and eight (51 ± 2.91 and 17 ± 2.23, respectively, p < 0.01) days after spraying. At day 16th, post treatment, abamectin had the highest residual mortalities on O. albidipennis (15 ± 1.58, p < 0.01) and finally mortality results at the 20th day showed there is no significant difference between insecticides (p < 0.01).     

Effects of chronotype and time of day on mood responses to CrossFit training

Morningness-eveningness, time of day, and physical exercises have been shown to influence mood states. This study aimed to test whether impact of physical exercise on mood depends on time of day and chronotype. Ninety-four participants (age 32 ± 6 years; 34% females; weekly training volume 4 ± 1 hours) filled the Composite Scale of Morningness and reported their current mood using the UWIST Mood Adjective Checklist before and after a 60-min long CrossFit training session which took part in the morning (beginning at 6:30 or 7:45) or in the evening (beginning at 19:30 or 20:45). In this quasi-experiment the measurements were taken by the occasion of the participants’ usual training, in their preferred hours. There were only a few evening-types in the studied sample, while morning and intermediate chronotypes were over-represented. Participation in CrossFit training resulted in mood improvement consisting of increase in energetic arousal (η² = 0.29) and hedonic tone (η² = 0.47) and reduction of tense arousal (η² = 0.14), all significant at p = 0.001. Furthermore, CrossFit training during morning hours boosted mood in the intermediate/evening chronotype group to the levels observed in morning chronotypes (η² = 0.29, p < 0.05, for the three-way interaction effect). We conclude that participation in intense physical exercise, such as CrossFit training, may allow compensation for the negative effects of non-optimal time of day on experienced moods, particularly in the case of neither/evening-types.     

Melatonin ingestion after exhaustive late-evening exercise improves sleep quality and quantity,and short-term performances in teenage athletes

ABSTRACT

The present study aimed to explore the effects of a single 10-mg dose of melatonin (MEL) administration after exhaustive late-evening exercise on sleep quality and quantity, and short-term physical and cognitive performances in healthy teenagers. Ten male adolescent athletes (mean ± SD, age = 15.4 ± 0.3 years, body-mass = 60.68 ± 5.7 kg, height = 167.9 ± 6.9 cm and BMI = 21.21 ± 2.5) performed two test sessions separated by at least one week. During each session, participants completed the Yo-Yo intermittent-recovery-test level-1 (YYIRT-1) at ~20:00 h. Then, sleep polysomnography was recorded from 22:15 min to 07:00 h, after a double blind randomized order administration of a single 10-mg tablet of MEL (MEL-10 mg) or Placebo (PLA). The following morning, Hooper wellness index was administered and the participants performed the Choice Reaction Time (CRT) test, the Zazzo test and some short-term physical exercises (YYIRT-1, vertical and horizontal Jumps (VJ; HJ), Hand grip strength (HG), and five-jump test (5-JT)). Evening total distance covered in the YYIRT-1 did not change during the two conditions (p > 0.05). Total sleep time (Δ = 24.55 mn; p < 0.001), sleep efficiency (Δ = 4.47%; p < 0.001), stage-3 sleep (N3 sleep) (Δ = 1.73%; p < 0.05) and rapid-eye-movement sleep (Δ = 2.15%; p < 0.001) were significantly higher with MEL in comparison with PLA. Moreover, sleep-onset-latency (Δ = –8.45mn; p < 0.001), total time of nocturnal awakenings after sleep-onset (NA) (Δ = –11 mn; p < 0.001), stage-1 sleep (N1 sleep) (Δ = –1.7%; p < 0.001) and stage-2 sleep (N2 sleep) (Δ = ?1.9%; p < 0.05) durations were lower with MEL. The Hooper index showed a better subjective sleep quality, a decrease of the subjective perception of fatigue and a reduced level of muscle soreness with MEL. Moreover, MEL improved speed and performance but not inaccuracy during the Zazzo test. CRT was faster with MEL. Morning YYIRT-1 (Δ = 82 m; p < 0.001) and 5-JT (Δ = 0.08 m; p < 0.05) performances were significantly higher with MEL in comparison with PLA. In contrast, HG, VJ and HJ performances did not change during the two conditions (p > 0.05). The administration of a single dose of MEL-10 mg after strenuous late-evening exercise improved sleep quality and quantity, selective attention, subjective assessment of the general wellness state, and some short-term physical performances the following morning in healthy teenagers.     

Effect of resistance training during the month of Ramadan on antioxidants and oxidative stress biomarkers in recreational bodybuilders

The aim of this study was to investigate the effect of a hypertrophic training program during Ramadan on levels of circulating antioxidants and oxidative stress biomarkers in trained bodybuilders. Eight male bodybuilders visited the laboratory two days before the start of Ramadan and on the 29th day of Ramadan (end-Ramadan), to undergo anthropometric measurement, to complete a dietary questionnaire, and to provide fasting blood and urine samples. Plasma thiobarbituric acid reactive substance and protein-bound carbonyl concentrations were measured in the plasma to indicate lipid and protein oxidation, respectively. These did not change significantly during Ramadan. Superoxide dismutase and glutathione peroxidase activities, indices of erythrocytes antioxidant status, increased by 24% (p = 0.011) and 12% (p = 0.01), respectively, from before to the end of Ramadan. However, erythrocyte catalase activity did not change significantly. Uric acid values increased by 17% (p < 0.001) from before to the end of Ramadan, but ferric-reducing ability and Vitamin E levels of the plasma remained unchanged. A significant correlation was found between uric acid values and urine specific gravity (r = 0.70; p < 0.05) only at the end of Ramadan. We conclude that continuing a hypertrophic training program during Ramadan fasting does not increase oxidative stress but does increase antioxidant status in bodybuilders.     

Methionine,folic acid and vitamin B12 in growing-finishing pigs: Impact on growth performance and meat quality

Growth performance, metabolic variables, and meat quality were measured in 78 growing-finishing pigs using supplements of 0 (C), or 0.2% of DL-methionine (M), and three combinations of folic acid [mg/kg] and cyanocobalamin [μg/kg], respectively 0 and 0 (V0), 10 and 25 (V1), and 10 and 150 (V2) in a 2 × 3 factorial arrangement. Feed conversion was lower (p = 0.05) in M than in C pigs during the growing period (0–4 weeks). Both V1 and V2 treatments increased plasma vitamin B₁₂ (p < 0.01) and decreased plasma homocysteine (p < 0.01). Plasma 5-methyl-tetrahydrofolates were the lowest, highest and intermediate in V0, V1 and V2 pigs (p < 0.04), respectively. In V2 meat, folates were 32% higher, vitamin B₁₂, 55% higher and homocysteine, 28% lower than in V0 (p < 0.01). Oxidative stability of the fresh meat was similar among treatments during a storage period of 42 days. Therefore, methionine supplements improved growth performance during the growing period. Vitamin supplements interacted with the methionine cycle pathway, increased vitamin content of pork meat but did not improve oxidative stability of the fresh meat during storage.     

Effects of an active warm-up on variation in bench press and back squat (upper and lower body measures)

The present study investigated the magnitude of diurnal variation in back squat and bench press using the MuscleLab linear encoder over three different loads and assessed the benefit of an active warm-up to establish whether diurnal variation could be negated. Ten resistance-trained males underwent (mean ± SD: age 21.0 ± 1.3?years, height 1.77 ± 0.06?m, and body mass 82.8 ± 14.9?kg) three sessions. These included control morning (M, 07:30?h) and evening (E, 17:30?h) sessions (5-min standardized warm-up at 150?W, on a cycle ergometer), and one further session consisting of an extended active warm-up morning trial (M_E, 07:30?h) until rectal temperature (T_rec) reached previously recorded resting evening levels (at 150?W, on a cycle ergometer). All sessions included handgrip, followed by a defined program of bench press (at 20, 40, and 60?kg) and back squat (at 30, 50, and 70?kg) exercises. A linear encoder was attached to an Olympic bar used for the exercises and average force (AF), peak velocity (PV), and time to peak velocity (tPV) were measured (MuscleLab software; MuscleLab Technology, Langesund, Norway) during the concentric phase of the movements. Values for T_rec were higher in the E session compared to values in the M session (Δ0.53?°C, P < 0.0005). Following the extended active warm-up in the morning (M_E), T_rec and T_m values were no different to the E values (P < 0.05). Values for T_m were lower in the M compared to the E condition throughout (P < 0.05). There were time-of-day effects for hand grip with higher values of 6.49% for left (P = 0.05) and 4.61% for right hand (P = 0.002) in the E compared to the M. Daily variations were apparent for both bench press and back squat performance for AF (3.28% and 2.63%), PV (13.64% and 11.50%), and tPV (?16.97% and ?14.12%, where a negative number indicates a decrease in the variable from morning to evening). There was a main effect for load (P < 0.0005) such that AF and PV values were larger at higher masses on the bar than lower ones and tPV was smaller at lower masses on the bar than at higher masses for both bench press and back squat. We established that increasing T_rec in the M–E values did not result in an increase of any measures related to bench press and back squat performance (P > 0.05) to increase from M to E levels. Therefore, MuscleLab linear encoder could detect meaningful differences between the morning and evening for all variables. However, the diurnal variation in bench press and back squat (measures of lower and upper body force and power output) is not explained by time-of-day oscillations in T_rec.     

Opuntia ficus-indica juice supplementation: what role it plays on diurnal variation of short-term maximal exercise?

The aim of this study was to investigate the effect of natural Opuntia ficus-indica juice (OFIJ) supplementation on anaerobic performance at two times of day. Twenty-two healthy male subjects (20.91 ± 1.22; 21.00 ± 0.84 years) divided into two groups: Experimental group (EG: n = 11) and a control group (CG: n = 11) performed two tests-sessions (30-s of Wingate test (i.e. Peak power (PP), Mean power (MP)), Sargent jump test (SJT), sprint 10 m), before and after natural OFIJ supplementation at 07:00 h and 17:00 h. T-test showed that the OFIJ has a potent antioxidant capacity for capturing free radicals following the 22-diphenyl-1-picrylhydrazyl (DPPH^●) test (p < 0.05). Likewise, the ANOVA revealed that anaerobic performances were significantly higher at 17:00 h compared to 07:00 h around the peak of the temperature (p < 0.05) in both EG and CG before supplementation. Moreover, OFIJ lead an improvement of performances with (+2.09% at 07:00 h vs.+9.36% 17:00 h) for PP, (+11.29% at 07:00 h vs.+11.77% 17:00 h) for MP, (+9.42% at 07:00 h vs.+7.63% 17:00 h) for SJT in EG. The RPE scores on response to the Wingate test decrease after OFIJ supplementation (p < 0.01). For the sprint values, a significant improvement was after OFIJ (?7.10% at 07:00 h vs. ?6.45% 17:00 h). However, no change was observed for CG after supplementation. In conclusion, the natural OFIJ supplementation for two weeks appears to ameliorate the performance upon two times of day with great improvement observed in the evening during short-term maximal exercise given the higher muscle damage, inflammatory, and oxidative responses at this time of day. Thus, it’s necessary that athletes, coaches, and medical staff consider the positive effects of Opuntia ficus-indica to improve anaerobic performance.     

Evening chronotype is associated with poor cardiovascular health and adverse health behaviors in a diverse population of women

ABSTRACT

Chronotype reflects time of day preferences for performing daily activities. Previous research within Asian and European cohorts indicates evening chronotype is associated with elevated cardiometabolic risk. However, evidence is limited from population-based US cohorts, particularly among women in whom evening chronotype prevalence may become higher after middle-age, coinciding with life stages associated with higher cardiovascular disease (CVD) risk. This cross-sectional study evaluated associations of chronotype with overall cardiovascular health (CVH), health behaviors, and cardiometabolic risk factors among 506 women (mean age = 37 ± 16y, 62% racial/ethnic minority) in the American Heart Association (AHA)’s Go Red for Women Strategically-Focused Research Network cohort at Columbia University (New York City, NY, USA). Chronotype was assessed using the validated Morningness-Eveningness Questionnaire (MEQ) and categorized as “evening”, “intermediate”, and “morning” chronotypes. Health behaviors (diet, physical activity, and sleep) were assessed using validated questionnaires. Anthropometrics, clinical blood pressure, and blood biomarkers were assessed at the clinic visit. CVH was evaluated using the AHA Life’s Simple 7 (LS7) metrics; LS7 scores of 0–8 and 9–14 were considered indicative of poor and moderate-to-high CVH, respectively. Linear and logistic regression models adjusted for age, race/ethnicity, education, health insurance, and menopausal status were used to examine associations of MEQ scores and chronotype categories with overall CVH, clinical cardiometabolic risk factors, and health behaviors. Overall, 13% of women identified as evening chronotypes, while 55% and 32% reported being intermediate and morning types. In linear models, higher MEQ scores were associated with higher AHA LS7 scores (β(SE) = 0.02(0.01); p = .014), indicative of more favorable CVH, and with health behaviors not included in the LS7. Higher MEQ scores were also associated with lower Pittsburgh Sleep Quality Index, i.e. better sleep quality, (β(SE) = ?0.07(0.02), p < .0001), lower insomnia severity (β(SE) = ?0.14(0.01), p < .0001), shorter time to fall asleep (β(SE) = ?0.28(0.14), p = .044), and less sedentary time (β(SE) = ?0.11(0.03), p = .001). In logistic regression models, evening chronotype, compared to intermediate/morning type, was associated with higher odds of having poor CVH (OR(95%CI):2.41(1.20–4.85)), not meeting AHA diet (OR(95%CI):2.89(1.59–5.23)) and physical activity guidelines (OR(95%CI):1.78(1.03–3.07)), and having short sleep (OR(95%CI):2.15(1.24–3.73)) or insomnia (OR(95%CI):2.69(1.53–4.75)). The evening type compared to morning type was also associated with being a current smoker (OR(95%CI):2.14(1.02–4.52)) and having poor sleep quality (OR(95%CI:2.35(1.27–4.37)) and long sleep onset latency (OR(95%CI:1.89(1.00–3.56)). In our cohort of women, evening chronotype was related to poor CVH, likely driven by its influence on health behaviors. These findings, although warranting confirmation prospectively in other populations, suggest chronotype is an important factor to consider and possibly target when designing lifestyle interventions for CVD prevention.     

Day-Night Differences in the Kinetics and Dynamics of Insulin: Diabetic Versus Normal Yucatan Minipigs

Time-dependent variations in the pharmacokinetics and pharmacodynamics of insulin were studied at two times, 10:30 and 20:30 during the same day in normal and streptozotocin (STZ)-induced diabetic minipigs housed in L(06:00):D(18:00) using the intravenous insulin tolerance test. Following intravenous insulin (0.1 IU/kg) administration in normal minipigs, the time for the glucose level to reach nadir (t_nadir) was significantly longer in the evening than the morning [(a.m.: 30.4 (± 2.4) VS. P.M.: 38.5 (± 3.3) min] (p < 0.01), although maximum reduction of glucose level (nadir) in the morning and evening was not significantly different [a.m.: (-70 (± 2) VS. P.M.: -65 (± 5) %]. The rate of glucose decline (K_in) was significantly decreased in the evening [a.m.: 5.33 (± 0.71) VS. P.M.: 4.44 (± 0.54) %dBG/min] (p < 0.01), and the integrated glucose-lowering response (ABCB) was significantly higher in the evening than the morning [a.m.: 3.18 (± 0.38) VS. P.M.: 4.52 (± 0.30) (g/dl) * min] (p < 0.01). The area under the plasma insulin concentration curve was increased significantly in the evening [a.m.: 2.26 (± 0.174) VS. P.M.: 2.74 (± 0.18) (mU/ml) * min], while the morning plasma insulin half-life did not differ significantly from that in the evening [a.m.: 4.79 (± 0.36) VS. P.M.: 5.47 (± 0.47) min]. After induction of diabetes by intravenous STZ injections, minipigs became diabetic, baseline blood glucose was observed to increase from the range of 45–55 to 200–250 mg/dl while plasma insulin levels decreased from 7–12 to 3–5 uU/ml. In the STZ-induced diabetic minipigs, a higher dose (0.2 IU/kg) was used in the intravenous insulin tolerance test in an attempt to normalize the high glucose levels. Following intravenous     

Preliminary findings for the validity of the Morningness–Eveningness-Stability Scale improved (MESSi): Correlations with activity levels and personality

Aim of the present study is an additional validation of the Morningness–Eveningness-Stability Scale improved (MESSi). We screened a total of 97 German students using the reduced Morningness–Eveningness Questionnaire (rMEQ) to identify a subsample (N = 42) of definite morning and evening types (31% males, mean age: 24.8 ± 5.8?years). The participants provided information about their sleep–wake rhythm (diary), personality traits (questionnaire) and experienced actigraphic monitoring. Correlations of the MESSi components “Morning affect subscale” (MA) (r = 0.91, p < 0.01) and “Eveningness subscale” (r = ?0.87, p < 0.01) with the rMEQ showed good convergent validity. MA was also significantly negatively correlated with the acrophase and the midpoint of sleep as measured by actigraphy.     

Post-resistance training detraining: time-of-day effects on training and testing outcomes

The aim of this study was to examine the effects of 3 and 5 weeks of detraining after 14 weeks of resistance training at a specific time of day on performances during the squat jump (SJ) and the maximal voluntary contraction (MVC). Thirty-one healthy male physical education students (age: 23.1 ± 1.0 years; height: 176.1 ± 6.3 cm; weight: 74.9 ± 10.9 kg) were randomly assigned to either a morning training group (MTG, training between 07:00 and 08:00 h, n = 10), an evening training group (ETG, training between 17:00 and 18:00 h, n = 11) or a control group (CG, no training, n = 10). Participants then performed eight test sessions (twice per day, at 07:00 and 17:00 h) over the course of four phases: during pre-training, immediately post-training, and after 3 and 5 weeks of detraining. Before each test session, oral temperature was recorded. During the first 12 weeks of resistance training, participants performed 3 sets of 10 repetitions to failure (10-RM) for 4 exercises (squat, leg press, leg extension and leg curl, with 2 min of recovery between each exercise); during the last two weeks, training intensity increased to 8-RM with 3 min of recovery between each exercise. Oral temperature was significantly higher at 17:00 than 07:00 h during all test periods (p < 0.05). Likewise, SJ and MVC performances were significantly higher at 17:00 h than 07:00 h during all four test days in ETG and CG, and before training and 3 and 5 weeks after training in MTG (p < 0.05). For both training groups, most SJ and MVC performances (except MTG at 07:00 h and ETG at 17:00 h) returned to baseline values after 5, but not after 3, weeks of detraining. This study showed that 14 weeks of training at a specific time of day blunted the diurnal variation of MVC and SJ in the MTG. The improvement in performance brought about by resistance training was partially retained after 3 weeks of detraining (unless training had taken place at a non-habitual time of day) but was lost after 5 weeks of detraining. There was no effect of the time of training on core temperature.     

Effect of sport practice and warm-up duration on the morning–evening difference in anaerobic exercise performance and perceptual responses to it

This study was designed to assess the effect of sport practice and warm-up duration on the morning–evening differences in muscle power and fatigue during performance of anaerobic exercise and perceptual responses to it. Twenty-two male physical education students – twelve trained (TG) (21.51 ± 1.25 years, 182.17 ± 4.37 cm and 82.88 ± 11.23 kg) and ten untrained (NTG) (23.89 ± 3.17 years, 176.8 ± 2.2 cm and 82.24 ± 8.43 kg) – participated in a crossover randomized study. They were asked to perform a 30-s Wingate test during six experimental sessions (three at 08:00 and three at 18:00 h) after different active warm-up (AWU) durations (5 min, 15 min, or 20 min). The AWU consisted of pedaling at a constant pace of 60 rpm against 50% of maximal aerobic power. Rate of perceived exertion (RPE) was recorded after the AWU and again immediately after the Wingate test. Heart rate and temperature (T) were recorded during each session at rest, after AWU and immediately at the end of the Wingate test. During the Wingate test, peak power (PP), mean power (MP), and the fatigue index were recorded. While the RPE estimations were higher in NTG, no time-of-day effect was recorded in either experimental group (morning or evening). T, PP, and MP were higher in the afternoon than in the morning (p < 0.001 for PP and MP; p < 0.05 for T). Similarly, PP and MP during the Wingate test were significantly higher in the TG than in the NTG (p < 0.001). The morning–evening difference of PP and MP was affected by AWU duration; AWU₁₅ was best in the morning for improving lower limb power for both the TG and NTG, whereas reducing this period to 5 min in the evening was appropriate for TG only.     

Modulating effect of dopamine on amplitude of GABA-produced chemocontrolled currents in multipolar spinal cord neurons of ammocaete

By using the patch-clamp method in the whole cell configuration, modulating effect of dopamine on GABA-activated currents has been studied on isolated multipolar spinal cord neurons of the ammocaete (larva of the lamprey Lampetra planeri). At application of dopamine (5 μM), there was observed in some cases a decrease of the GABA-activated current, on average, by 33.3 ± 8.7% (n = 8, p < 0.01), in other cases—an increase of the amplitude, on average, by 37.3 ± 11.8% (n = 5, p < 0.01). Concentration of GABA amounted to 2 mM. Study of action of agonists of D1- and D2-receptors on amplitude of chemocontrolled currents has shown that agonist of D1-receptors (+)-SKF-38393 (5 μM) decreases the GABA-activated current amplitude, on average, by 63.1 ± 11.7% (n = 8, p s< 0.01); the agonist of D2-receptors (−)-quinpirole (5 μM) produces in various cells the dopamine-like effects: an increase of the GABA-activated current amplitude, on average, by 61.0 ± 13.8% (n = 8, p < 0.01) and a decrease of amplitude, on average, by 55.7 ± 2.0% (n = 6, p < 0.01). It has been shown that antagonist of D2-receptors sulpiride (5 μM) does not block effects produced by dopamine. The dopamine effects were partially blocked by antagonist of D1-receptors (+)-SCH-23390 (5 μM): a decrease of the GABA-activated amplitude current amounted, on average, to 11.7 ± 1.8% (n = 7, p < 0.01), while an increase of amplitude—8.3 ± 2.0% (n = 5, p < 0.01). At the same time, effects of agonist of D1-receptors quinpirole (5 μM) were partially blocked by antagonist of D1-receptors (+)-SCH-23390: a decrease of the GABA-activated current amplitude amounted, on average, to 9.2 ± 3.4% (n = 6, p < 0.01) and an increase of amplitude—6.3 ± 1.8% (n = 10, p < 0.01). The obtained data indicate differences of mechanisms of the receptor-mediated effect of agonists of dopamine receptors on GABA-activated and potential-activated currents of multipolar neurons of the ammocaete spinal cord.     

Effect of napping opportunity at different times of day on vigilance and shuttle run performance

ABSTRACT

The aim of the present study was to examine the effect of a nap opportunity during the daytime realized at different times of day on physical and mental performance. Eighteen physically active males (age: 20.5 ± 3.0 years, height: 175.3 ± 5.9 cm, body-mass: 70.0 ± 8.6 kg) were tested under four experimental conditions: no-nap condition, nap at 13h00, nap at 14h00 and nap at 15h00. All nap durations were of 25-min and all tests were performed at 17h00. They performed a 5-m shuttle run test, which generated measures of the highest distance (HD) and total distance (TD). The rating of perceived exertion (RPE) was recorded after each of the six sprints in the 5-m shuttle run test. Vigilance was measured using a digit cancellation test. The results showed that TD at 17h00 was 4% greater after a nap at 14h00 than in the no-nap condition (+28 m, p < .05) or after the nap at 13h00 (+29 m, p < .05). HD was 8% higher (+9 m, p < .001) after a nap at 14h00 than in the no-nap condition and 7% higher after nap at 15h00 than in the no-nap condition (+7 m, p < .05). In addition, HD was 6% higher after nap at 14h00 (+7 m, p < .01) and 5% higher after nap at 15h00 (+9 m, p < .01) than HD after a nap at 13h00. Napping at 13h00 had no effect on physical performance at 17h00. No significant differences were observed between RPE and vigilance scores in the nap and no-nap conditions. In conclusion, napping for 25 min at 14h00 and 15h00 produces meaningful improvements in responses during repeated short-term maximal exercise tests performed at 17h00. Napping at 13h00 does not. Vigilance, as measured using a digit cancellation test, and RPE scores are not influenced by any of the nap opportunities.     

Sleep patterns and nocturnal cardiac autonomic activity in female athletes are affected by the timing of exercise and match location

The aim of the current study was to analyze the influence of the timing of training and matches, and match location, on sleep pattern and nocturnal cardiac autonomic activity in female soccer players. A total of 17 female soccer players (age: 21.6 ± 2.3 years; mean ± SD) wore wrist actigraph units and heart rate (HR) monitors during night-sleep throughout 18 night-training days (NTD), 18 resting days (RD), and 6 match-days (MD; 3 away-matches [AM] and 3 home-matches [HM]) of the competitive period. Training load was quantified by session rating of perceived exertion (sRPE), heart rate (HR), training impulse (TRIMP), and subjective well-being. In NTD, sleep onset time and total sleep time were significantly impaired compared to MD (+ 1:47 [0:48; 1:55] hours and ?1:35 [?1:42; ?0:57] hours, respectively; p< 0.001; mean difference ± 95% confidence interval [CI]) and RD (+ 1:31 [0:52; 1:49] hours and ?1:26 [?1:28; ?0:55] hours, respectively; p< 0.001). In AM, sleep onset time was delayed compared to HM (+ 0:36 [0:12; 0:44] hours; p< 0.001). Sleep latency was higher in NTD compared to RD (+ 4 [2; 5] min; p< 0.001), as well as after AM compared to HM (+ 5 [1; 7] min; p< 0.001). HR during sleep was significantly increased after NTD and MD compared to RD (+ 4 [1; 5] b.p.m and + 3 [1; 4] b.p.m, respectively; p< 0.001). Furthermore, HR variability decreased after NTD and MD compared to RD (e.g., lnRMSSD, ?0.09 [?0.18; ?0.08] ln[ms] and ?0.17 [?0.27; ?0.11] ln[ms], respectively; p< 0.001). Overall, the time of day for soccer practice (i.e., training at night) and match location (i.e., away matches) may cause disruption in sleep patterns and/or in autonomic cardiac activity in female soccer players. Additionally, playing official matches during the day and training at night may affect nocturnal cardiac autonomic activity by decreasing vagal-related modulation and increasing HR during sleep.     

The effect of time of day on short-term maximal performance in obese and non-obese young men

The purpose of this study was to examine time-of-day effects on short-term performance in obese young men. In a balanced study design, 30 boys were divided into two groups: obese (OG; n = 15) and non-obese (CG; n = 15) groups. Both groups performed anaerobic tests of strength and power (squat-Jump, SJ; counter-movement jump, CMJ; 10-m sprint; and 30-m sprint) at 08:00 and 17:00 h on separate days. The results showed morning–evening differences in all variables in CG, with significant increases at 17:00 h (around the time of peak temperature) in comparison with 08:00 h (p < 0.01) for OG. However, such morning-evening differences were not found in the OG, except for the 30-m sprint. Obesity affects the diurnal variation on performances of 30-m sprint with a significant decrease in the evening for OG of 1.23% (p < 0.01). A correlation between anaerobic performance and oral temperature was observed only in the 30-m sprint test. In conclusion, the findings of the present study indicated that obesity is a problem that can affect circadian rhythms and performance in obese young men; therefore, preventing childhood obesity should become a top priority in efforts to improve performance and health.     

Nutritional and physiological responses of finishing pigs exposed to a permanent heat exposure during three weeks

The aim of the current study was to investigate the effect of a permanent heat exposure during 21 days on pig performance, nutrient digestibility, physiological response and key enzyme of skeletal muscle energy metabolism. Twenty-four male finishing pigs (crossbreed castrates, 79.0 ± 1.50 kg body weight) were allocated to three groups (n = 8): (1) Control (ambient temperature (AT) 22°C, ad libitum feeding), (2) Group HE (AT 30°C, ad libitum feeding) and (3) Group PF (AT 22°C, pair-fed to Group HE). The permanent heat exposure decreased feed intake (p < 0.01), daily body weight gain (p < 0.05) and the digestibility of gross energy, dry matter, crude protein and ash (p < 0.05); rectal temperature and respiration rate were significantly increased (p < 0.01). The levels of plasma cortisol, creatine kinase and lactate dehydrogenase were also significantly increased in Group HE (p < 0.05). Furthermore, the heat exposure changed intracellular energy metabolism, where the AMP-activated protein kinase was activated (p = 0.02). This was combined with changes in parameters of glycolysis such as an accumulation of lactic acid (p = 0.02) and a drop of pH_24?h (p = 0.02), an increase of hexokinase and pyruvate kinase activity (p < 0.01) and, finally, the maturation process of post mortem muscle was influenced. Due to pair-feeding it was possible to evaluate the effects of heat exposure, which were not dependent on reduced feed intake. Such effects were, e.g., reduced nutrient digestibility and changed activities of several enzymes in muscle and blood serum.     

Daily rhythms in activity and mRNA abundance of enzymes involved in glucose and lipid metabolism in liver of rainbow trout,Oncorhynchus mykiss. Influence of light and food availability

ABSTRACT

We have investigated the magnitude of diurnal variation in back squat and bench press performance using the MuscleLab force velocity transducer. Thirty resistance-trained males (mean ± SD: age 21.7 ± 1.4 years; body mass 80.5 ± 4.5 kg; height 1.79 ± 0.06 m) underwent two sessions at different times of day: morning (M, 07:30 h) and evening (E, 17:30 h). Each session included a period when rectal temperature (T_rec) was measured at rest, a 5-min standardized 150 W warm-up on a cycle ergometer, then defined programme of bench press (at 20, 40 and 60 kg) and back squat (at 30, 50 and 70 kg) exercises. A linear encoder was attached to an Olympic bar used for the exercises and average force (AF), peak velocity (PV) and time-to-peak velocity (tPV) were measured (MuscleLab software; MuscleLab Technology, Langesund, Norway) during the concentric phase of the movements. Values for T_rec at rest were higher in the evening compared to morning values (0.48°C, P < 0.0005). Daily variations were apparent for both bench press and back squat performance for AF (1.9 and 2.5%), PV (8.3 and 12.7%) and tPV (?16.6 and ?9.8%; where a negative number indicates a decrease in the variable from morning to evening). There was a main effect for load where AF and tPV increased and PV decreased from the lightest load to the heaviest for both bench press and back squat (47.1 and 80.2%; 31.7 and 57.7%; ?42.1 and ?73.9%; P < 0.0005 where a negative number indicates a decrease in the variable with increasing load). An interaction was found only for tPV, such that the tPV occurs earlier in the evening than the morning at the highest loads (60 and 70 kg) for both bench press and back squat, respectively (mean difference of 0.32 and 0.62 s). In summary, diurnal variation in back squat and bench press was shown; and the tPV in complex multi-joint movements occurs earlier during the concentric phase of exercise when back squat or bench press is performed in the evening compared to the morning. This difference can be detected using a low cost, portable and widely available commercial instrument and enables translation of past laboratory/tightly controlled experimental research in to main-stream coaching practice.