3H-BA在花生叶片的吸收和分布

花生叶圆片对^3H-BA的吸收方式,以被动吸收为主,也有主动吸收,叶圆片在^3H-BA溶液中温育2h,吸收BA最多,以后则下降。PH7．5的介质最适合^3H-BA的吸收。在16-26℃范围内,吸收BA随温度的上升而增加。^3H-BA进入花生幼苗体内后,移动性小。标记^3H-BA 6d后,滞留率仍达97．3%。     

4PU—30对水稻叶片的保绿效应

4PU—30对水稻离体叶圆片和抽穗后整株叶片的衰老均有明显的延缓效应,在离体叶圆片培养中其效应比6—BA大。     

细胞壁中富含羟脯氨酸蛋白质与烟草叶细胞扩大生长的关系

从生长良好的烟草叶钻下圆片进行离体培养。发现在含有2 ppm 6BA的MS培养液中加入游离的羟脯氨酸,能明显地抑制烟草叶圆片的扩大生长.羟脯氨酸浓度在0.05 mM到0.8 mM范围内与圆片的扩大生长呈显著负相关。用~(14)C-脯氨酸餵饲培养了3天的烟草叶圆片,发现在无激素的MS培养液中和在含有2 ppm 6BA+0.3 mM羟脯氨酸的MS培养液中生长缓慢的圆片,其放射性掺入圆片和掺入蛋白质的量均低于在含2 ppm 6BA的MS培养液中迅速生长的圆片。然而,生长缓慢的圆片,其细胞质水溶性部分和细胞壁部分羟脯氨酸/脯氨酸之比值却都高于迅速生长的圆片。这意味着富含羟脯氨酸蛋白质的大量形成可能不利于细胞的扩大生长。细胞壁中放射性的掺入量随餵饲时间的延长而增加,羟脯氨酸/脯氨酸比值也随餵饲时间的延长而提高,这说明在细胞壁中亦可能有脯氨酸的羟基化过程发生。     

BA对花生光合产物分配,积累和碳水化合物的影响（简报）

用BA10~(-5)mol/L或5mgBA/g羊毛脂涂抹花生叶片,促进~(14)C-光合产物向其处理部位分配和积累,提高~(14)CO_2标记叶光合产物的输出能力,蔗糖含量提高63％,淀粉降低26.4％,后者主要通过淀粉酶完成。     

花生幼叶为外植体的植株再生系统的建立

本文报道利用花生成熟胚幼叶为外植体获得高频植株再生的方法,为花生转基因提供有效的受体系统。通过诱导培养基TDZ、BA、NAA的浓度以及种子萌发时间、继代培养基种类五个因素不同水平的正交试验,筛选出了分化高频发生的最佳组合为：MS培养基中应含有TDZ 1.0 μmol/L、BA 0.4 μmol/L、NAA 5.0 μmol/L,种子萌发4 d,继代培养基为MS0。本研究表明,五因素中诱导培养基TDZ浓度为诱导花生幼叶分化的主要影响因素,其次为继代培养基、种子萌发时间,而诱导培养基中BA和NAA的浓度作用较小。试管苗生根后移栽田间,可正常开花结果。     

花生幼叶为外植体的植株再生系统的建立

本文报道利用花生成熟胚幼叶为外植体获得高频植株再生的方法,为花生转基因提供有效的受体系统。通过诱导培养基TDZ、BA、NAA的浓度以及种子萌发时问、继代培养基种类五个因素不同水平的正交试验,筛选出了分化高频发生的最佳组合为：MS培养基中应含有TDZ 1．0／μmol／L、BA0．4μmol／L、NAA5．0μmol／L,种子萌发4d,继代培养基为MS0。本研究表明,五因素中诱导培养基TDZ浓度为诱导花生幼叶分化的主要影响因素,其次为继代培养基、种子萌发时问,而诱导培养基中BA和NAA的浓度作用较小。试管苗生根后移栽田间,可正常开花结果。     

用显微放射自显影技术对~(14)C标记B_9在花生各器官中分布与积累的研究

在花生花芽分化期、始花期,用~(14)C标记N,N-二甲基琥珀酰胺酸(B_9)涂布功能叶,显微放射自显影结果表明:B_9进入花生各器官非常迅速,处理后1小时,在茎、叶中就发现~(14)C-B_9踪迹。始花期处理后发现:~(14)C-B_9通过叶、叶柄和茎的维管束运输,其后,主要积累在同化组织茎的皮层和叶的栅栏组织中。~(14)C-B_9在花器官中首先在花丝、花柱和花瓣的维管束中出现,三天后大部分转移到花粉粒表面和浓集在花粉囊内壁。花芽分化期处理后发现大部分~(14)C-B_9踪迹进入花粉粒内。 花生植株提取的放射性物质的纸谱分析表明;B_9在花生体内比较稳定,不易分解,这可能是药效持久的一个原因。     

不同激素对花生离体分化的影响

对TDZ和2,4-D等激素在花生成熟胚外植体分化中的影响进行了研究.结果表明,花生成熟胚3～5 d龄实生苗的幼叶和胚轴在低浓度TDZ的诱导下,可分化产生高频不定芽和少量体细胞胚,转到无激素MS培养基或MS BA 0.5 mg/L NAA 0.4 mg/L的培养基后形成丛生苗.丛生苗分离后转入含1/2 MS(大量元素) IBA 0.4 mg/L的培养基中诱导生根,可形成完整的再生植株.幼叶分化率高于胚轴,但胚轴分化成苗速度快.无菌水浸泡16～24 h的胚轴在5～ 30 mg/L 2,4-D的诱导下,分化产生低频不定芽;而胚叶则产生高频体细胞胚,但畸形较严重.     

白桦脂酸对免疫性肝损伤小鼠细胞因子及Bcl-2、activated-Caspase-3表达的影响

目的研究白桦脂酸(BA)对刀豆蛋白(Con A)诱导的急性免疫性肝损伤小鼠细胞因子及凋亡相关蛋白Bcl-2、activated-Caspase-3表达量的影响。方法将60只雄性KM小鼠随机分为6组,包括正常对照组,肝损伤模型组,联苯双酯(BIF)阳性对照组,BA高、中、低剂量组(H-BA、M-BA、L-BA组剂量分别为30 mg·kg~(-1)、15 mg·kg~(-1)、7.5 mg·kg~(-1))。BIF阳性对照组和BA高、中、低剂量组预防性给药15 d后,尾静脉注射20 mg·kg~(-1)Con A构建小鼠急性免疫性肝损伤模型。采用自动生化分析仪测定血清肝功能指标:谷丙转氨酶(ALT)、谷草转氨酶(AST)含量;ELISA法测定血清炎性细胞因子IL-2、IL-4、IL-10、TNF-α、IFN-γ水平;Western blot法检测肝组织凋亡相关蛋白Bcl-2、activated-Caspase-3表达量的变化。结果与正常对照组比较,肝损伤模型组血清ALT和AST含量明显降低,IL-2、IL-4、IL-10和肿瘤坏死因子(TNF-α)、γ干扰素(IFN-γ)水平明显升高,Bcl-2表达量下降而activated-Caspase-3表达量升高,差异均有统计学意义(P0.05或P0.01);与肝损伤模型组比较,BA不同剂量组可显著降低Con A所致急性肝损伤小鼠血清ALT和AST含量,使小鼠血清中IL-2、IL-4、TNF-α、IFN-γ水平显著降低,而IL-10水平显著升高,尤其是H-BA组、M-BA组,同时使Bcl-2表达量升高、activated-Caspase-3表达量下降,差异均有统计学意义(P0.05或P0.01)。结论 BA对由Con A诱导的小鼠急性免疫性肝损伤具有拮抗作用。其机制可能与抗凋亡及降低炎性细胞因子水平和提高抗炎细胞因子水平、减轻T淋巴细胞毒性作用有关。     

断光后的急骤耗氧现象及其与光呼吸的关系(英文)

用氧电极研究叶圆片断光后的耗氧变化,观察到断光后急骤耗氧(rapid postilluminationoxygen consumption RPIOC)现象。在不更换反应液的情况下,随着连续测定次数的增加,烟草叶圆片和番薯叶肉细胞的RPIOC增强。烟草、水稻、番薯、木瓜、黄瓜的叶圆片和番薯叶肉细胞表现出RPIOC,而高粱、甘蔗和玉米则没有这种现象。烟草叶国片的RPIOC随以下因素变化而增强:氧浓度从0.077mmol/L 气相O_27％)增加到 0.230 mmol/L(气相O_2 21％)、光强从111W/m~2增加到350W/m~2、温度从20℃增加到40℃,NaHCO_3(pH7.8)浓度从1 mmol/L(气相CO_2 1188ppm)降低到0.05mmol/L(气相CO_260 ppm)。0.4 mmol/L的HPMS完全抑制烟草叶圆片的RPIOC;10mmol/L的环氧丙酸抑制烟草叶圆片的RPIOC 57.8％。因此认为RPIOC是光呼吸的另一种形式的表现,它对环境因子的变化与已知的影响光呼吸的因子具有一致的反应。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.