共查询到20条相似文献,搜索用时 31 毫秒
1.
2.
3.
4.
5.
6.
7.
Summary A broad-spectrum mercury resistance locus (mer) from a spontaneous chloramphenicol-sensitive (Cms), arginine auxotrophic (Arg–) mutant of Streptomyces lividan 1326 was isolated on a 6 kb DNA fragment by shotgun cloning into the mercury-sensitive derivative S. lividans TK64 using the vector pIJ702. The mer genes form part of a very large amplifiable DNA sequence present in S. lividans 1326. This element was amplified to about 20 copies per chromosome in the Cms Arg– mutant and was missing from strains like S. lividans TK64, cured for the plasmid SLP3. DNA sequence analysis of a 5 kb region encompassing the whole region required for broad-spectrum mercury resistance revealed six open reading frames (ORFs) transcribed in opposite directions from a common intercistronic region. The protein sequences predicted from the two ORFs transcribed in one direction showed a high degree of similarity to mercuric reductase and organomercurial lyase from other gram-negative and gram-positive sources. Few, if any, similarities were found between the predicted polypeptide sequences of the other four ORFs and other known proteins. 相似文献
8.
RNA expression analysis using a 30 base pair resolution Escherichia coli genome array 总被引:10,自引:0,他引:10
Selinger DW Cheung KJ Mei R Johansson EM Richmond CS Blattner FR Lockhart DJ Church GM 《Nature biotechnology》2000,18(12):1262-1268
9.
10.
11.
12.
13.
14.
FUT4 and FUT9 genes are expressed early in human embryogenesis 总被引:6,自引:0,他引:6
Cailleau-Thomas A Coullin P Candelier JJ Balanzino L Mennesson B Oriol R Mollicone R 《Glycobiology》2000,10(8):789-802
15.
16.
17.
18.
19.
20.