The WW Domain Protein Kibra Acts Upstream of Hippo in Drosophila

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Highlights? The WW domain protein Kibra is a Hippo signalling component ? Kibra restricts organ size via Yorkie and acts upstream of Hippo and Merlin ? Kibra synergizes with Expanded and binds to Merlin     

Tracking Shallow Chemical Gradients by Actin-Driven Wandering of the Polarization Site

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Highlights? A wandering polarity site distributes growth in yeast cells responding to pheromone ? Wandering, but not polarity establishment, is actin dependent ? A mathematical model incorporating polarity and vesicle traffic exhibits wandering ? Blocking wandering impairs gradient tracking     

Trachea-Derived Dpp Controls Adult Midgut Homeostasis in Drosophila

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Highlights? Trachea-derived Dpp/Bmp is required for midgut homeostasis ? Dpp expressed in tracheal cells reach intestinal cells through visceral muscles ? Dpp signaling is activated in enterocytes (ECs) in the Drosophila adult midgut ? Dpp signaling stabilizes ECs to restrict intestinal stem cell proliferation     

Interplay between the Dividing Cell and Its Neighbors Regulates Adherens Junction Formation during Cytokinesis in Epithelial Tissue

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Highlights? During cytokinesis, neighboring cells accumulate MyoII at the edges of the furrow ? MyoII nonautonomously sets the initial geometry of the daughter cell interface ? Neighboring membranes impede adherens junction (AJ) formation until a midbody forms ? Arp2/3-dependent actin accumulation in the dividing cell maintains AJ geometry     

The MRN-CtIP Pathway Is Required for Metaphase Chromosome Alignment

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Highlights? The MRN-CtIP pathway is required for metaphase chromosome alignment in egg extracts ? MRN inhibition interferes with spindle assembly around DNA-coated beads ? MRN inhibition in cells causes a metaphase delay and disrupts the RanGTP gradient ? The MRN complex regulates the stable association of RCC1 to chromatin     

Structure of a bacteriophytochrome and light-stimulated protomer swapping with a gene repressor

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Highlights? Bacteriophytochrome structure containing photosensory and output transducing domain ? RpBphP1 binds to the cognate repressor RpPpsR2 on illumination with far-red light ? HOS domain is distantly related to Dhp and is essential for binding to the repressor ? Light-induced protomer swapping mechanism between dimers     

Snx3 Regulates Recycling of the Transferrin Receptor and Iron Assimilation

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Highlights? Snx3 is highly expressed in vertebrate hematopoietic tissues ? Silencing of Snx3 results in anemia and hemoglobin defects in vertebrates ? Snx3 and Vps35 physically interact with Tfrc ? Snx3 is required for endosomal recycling of Tf-Tfrc complex     

Variable lymphocyte receptor recognition of the immunodominant glycoprotein of Bacillus anthracis spores

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Highlights? VLRBs use their C-terminal LRRs and the LRRCT-loop to interact with antigen ? Sequence-related VLRBs exhibit differential recognition of their BclA epitopes ? VLR4 binds a conserved protein epitope, yet is specific for B. anthracis spores     

Mucin Biopolymers Prevent Bacterial Aggregation by Retaining Cells in the Free-Swimming State

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Highlights? Mucin biopolymers reduce bacterial adhesion to underlying substrates ? Bacterial motility is maintained or increased in the presence of mucins ? Mucins block aggregate formation by motile bacteria ? Immotile Pseudomonas aeruginosa can form alginate and Psl-dependent flocs in mucus     

The Glove-like Structure of the Conserved Membrane Protein TatC Provides Insight into Signal Sequence Recognition in Twin-Arginine Translocation

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Highlights? Crystal structures of TatC from the thermophile Aquifex aeolicus are presented ? The architecture of TatC generates a glove-shaped pocket buried in the bilayer ? Molecular dynamics reveal destabilization of the membrane around the pocket ? Correlation to biochemical results suggest the signal sequence binds in this pocket     

Expression in Escherichia coli: becoming faster and more complex

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Highlights? Escherichia coli: a highly versatile host to produce samples for structural biology. ? High-throughput technologies have increased single protein expression success rates. ? Co-expression eases production of complexes but still suffers from bottlenecks. ? Co-expression combined with high-throughput can tackle complex assemblies.     

Evolutionary Shift from Fighting to Foraging in Blind Cavefish through Changes in the Serotonin Network

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Highlights? Astyanax cavefish have lost aggressive behavior during adaptation to life in the dark ? Astyanax raphe serotonergic neurons control social behavior ? Astyanax hypothalamic serotonergic neurons control homeostatic functions ? The development of a neuronal network links to the behaviors it controls     

A novel aux/IAA28 signaling cascade activates GATA23-dependent specification of lateral root founder cell identity

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Highlights? GATA23 is involved in lateral root founder cell specification ? A coordinated migration of pericycle nuclei precedes the first asymmetric division ? An Aux/IAA28-dependent auxin signaling cascade controls GATA23 expression     

Architecture of a dodecameric bacterial replicative helicase

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Highlights? Crystal structure of the ATPase domain of DnaB from Helicobacter pylori ? MALLS analysis shows that HpDnaB forms dodecamers ? EM reconstruction shows that HpDnaB forms head-to-head double hexamers     

The metabolic profile of tumors depends on both the responsible genetic lesion and tissue type

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Highlights? MYC and MET affect glucose and glutamine metabolism differently in the liver ? Tissue context affects the outcome of metabolic reprogramming by MYC ? MYC overexpression sensitizes cells to inhibition of Gls1 glutaminase     

Genetic Correction of a LRRK2 Mutation in Human iPSCs Links Parkinsonian Neurodegeneration to ERK-Dependent Changes in Gene Expression

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Highlights? hiPSC-derived neurons with LRRK2 G2019S exhibit Parkinsonian neurodegeneration ? Genetic correction of the LRRK2 mutation rescued neurodegenerative phenotypes ? Comparative analysis links LRRK2 G2019S, gene dysregulation, and ERK activation ? ERK-dependent gene dysregulation by LRRK2 G2019S contributes to neurodegeneration     

PRDM14 Ensures Naive Pluripotency through Dual Regulation of Signaling and Epigenetic Pathways in Mouse Embryonic Stem Cells

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Highlights? Prdm14 expression is important for maintenance of naive pluripotency ? PRDM14 antagonizes FGFR signaling and activates Akt-mTORC1 signaling ? PRDM14 represses de novo DNA methyltransferase expression ? This regulatory input is mediated through recruitment of PRC2     

Klf15 orchestrates circadian nitrogen homeostasis

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Highlights? Nitrogen homeostasis exhibits circadian rhythmicity in mammals ? Klf 15 regulates rhythmic amino acid utilization and ammonia detoxification ? Krüppel-like factor 15 is regulated by the circadian clock ? Feeding is a major external cue regulating Klf15 rhythmicity and nitrogen homeostasis     

ASH2L Regulates Ubiquitylation Signaling to MLL: trans-Regulation of H3 K4 Methylation in Higher Eukaryotes

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Highlights? ASH2L WH motif is important for trans-regulation of H3 K4 methylation by H2Bub ? Ub stimulates MLL activity in trans, in contrast to DOT1L regulation by H2Bub ? H2Bub enhances MLL, but not MLL3, methyltransferase activity ? H2Aub directly represses MLL methyltransferase activity