共查询到20条相似文献,搜索用时 8 毫秒
1.
Cho N Huh J Yang H Jeong EJ Kim YC Kim J Sung SH 《Journal of enzyme inhibition and medicinal chemistry》2012,27(1):1-4
A methanolic extract of the roots of Polygala tenuifolia (Polygalaceae) significantly attenuated nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated BV2 microglia cells. Five xanthones, 1-hydroxy-7-methoxyxanthone (1), 3,6-dihydroxy-1,2,7-trimethoxyxanthone (2), 1,3,6-trihydroxy-2,7-dimethoxyxanthone (3), 1,7-dihydroxy-2,3-dimethoxyxanthone (4) and 1,7-dihydroxy-3-methoxyxanthone (5), and five phenylpropanoids, 4-hydroxy-3-methoxypropiophenone (6), methyl 4-hydroxy-3-methoxycinnamic acid (7), 3,4,5-trimethoxycinnamic acid (8), 4-methoxycinnamic acid (9) and β-d-(3-O-sinapoyl) fructofuranosyl-α-d-(6-O-sinapoyl)glucopyranoside (10), were isolated from CHCl(3) fraction using bioactivity-guided fractionation. Among these compounds, compounds 1, 2, 4, 5 and 7 showed significant inhibitory effects on LPS-induced NO production in BV2 microglia cells at the concentration ranging from 10.0 to 100.0 μM. 相似文献
2.
Five previously undescribed 4,5-seco-abietane rearrange diterpenoids (1–5, Prionidipene A-E) were isolated from the aerial parts of Salvia prionitis, along with thirteen known seco-abietane diterpenoids (6–18). The structures of 1–5 were elucidated mainly based on analysis of NMR and MS data. The absolute configurations of 1–3 were determined by evaluation of experimental and calculated electronic circular dichroism (ECD) spectra. Putative biosynthetic pathways toward the formation of 1 and 2 are proposed. The nitric oxide (NO) production inhibitory effects of all isolates in lipopolysaccharide (LPS)-induced in RAW 264.7 cells were evaluated. Interestingly, compounds 4 and 9 with a furan-ring showed potent inhibitory activity with IC50 values of 14.56 and 15.11 μM, respectively. 相似文献
3.
Summary Hydroxyhydroquinone or 1,2,4-benzenetriol (BT) detected in the beverages has a structure that coincides with the water-soluble
form of a sesame lignan, sesamol. We previously showed that sesame antioxidants had neuroprotective abilities due to their
antioxidant properties and/or inducible nitric oxide synthase (iNOS) inhibition. However, studies show that BT can induce
DNA damage through the generation of reactive oxygen species (ROS). Therefore, we were interested to investigate the neuroprotective
effect of BT in vitro and in vivo. The results showed that instead of enhancing free radical generation, BT dose-dependently (10–100 μM) attenuated nitrite
production, iNOS mRNA and protein expression in lipopolysaccharide (LPS)-stimulated murine BV-2 microglia. BT significantly
reduced LPS-induced NF-κB and p38 MAPK activation. It also significantly reduced the generation of ROS in H2O2-induced BV-2 cells and in H2O2-cellfree conditions. The neuroprotective effect of BT was further demonstrated in the focal cerebral ischemia model of Sprague–Dawley
rat. Taken together, the inhibition of LPS-induced nitrite production might be due to the suppression of NF-κB, p38 MAPK signal
pathway and the ROS scavenging effect. These effects might help to protect neurons from the ischemic injury. 相似文献
4.
Delavatones A (4β, 7α-dihydroxy-7β-(13-methylpent-11-one-12-ene)-octahydrobenzofuran, 1) and B (4β, 7α-dihydroxy-7β-(propan-9-one)-octahydrobenzofuran, 2), two structurally unique cyclohexylethanol derivatives with a substituted alkyl chain, were isolated from the 90% ethanol extract of the whole plants of Incarvillea delavayi. The structures of delavatones A and B were characterized by the interpretation of 1D and 2D NMR spectroscopic data in combination with NMR chemical shift calculation coupled with DP4+ probability analysis. In an in vitro assay for anti-inflammatory effect, delavatones A and B displayed remarkable suppression on NO production in LPS-induced BV-2 cells at concentrations ranging from 6 μM to 100 μM. 相似文献
5.
《Bioorganic & medicinal chemistry letters》2014,24(24):5675-5678
Excessive NO (nitric oxide) has been associated with the pathogenesis of various neurodegenerative diseases including Alzheimer’s disease (AD). In our screening system using LPS-activated BV2 microglial cells, the methanolic extract of Disporum viridescens leaves was found to have significant NO inhibitory activity. Bioactivity-guided isolation yielded a new phenylpropanoid characterized as 4-ally-2,6-dimethoxyphenyl 1-O-β-d-apiofuranosyl (1 → 6)-β-d-glucopyranoside (12) with 21 known compounds from the leaves of D. viridescens. Among them, compounds 2 and 4 significantly inhibited NO production. Thus, we further elucidated the anti-inflammatory mechanism of these lignans. Especially, compound 4 inhibited the expression of both inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) through the suppression of the MAPK signaling pathway. Taken together, the anti-inflammatory activities of the active constituents isolated from D. viridescens leaves could have therapeutic potential against neurodegenerative diseases. 相似文献
6.
Eight new glycosides, morindaparvins P–W, were isolated from the butanol extract of the aerial parts of Morinda parvifolia. These new structures were elucidated by comprehensive spectroscopic analysis and by comparing the experimental and calculated electronic circular dichroism spectra. The butanol extract was observed to significantly reduce the levels of alanine aminotransferase, aspartate transaminase, and lactate dehydrogenase in the sera of mice with concanavalin A-induced hepatitis. At a concentration of 10 μM, five compounds (1, and 4–7) isolated from the butanol extract possessed hepatoprotective activities against the damage induced by acetaminophen in human HepG2 liver cancer cells. 相似文献
7.
In our survey on the chemical composition of Chinese folk medicines to further elucidate their chemical substances for the treatment of diseases, we investigated the chemical constituents of the plants Eupatorium fortunei. The investigation led to the isolation and identification of two new (1 and 2) and five known (3–7) thymol derivatives. Their structures were elucidated on the basis of extensive 1D and 2D NMR (COSY, HMQC, HMBC, and NOESY) and mass (ESIMS and HR-ESIMS) spectroscopic data analyses. The inhibitory activities on LPS-induced NO production of these compounds were also evaluated. 相似文献
8.
9.
Inhibitory effect of curcumin on nitric oxide production from lipopolysaccharide-activated primary microglia 总被引:7,自引:0,他引:7
Jung KK Lee HS Cho JY Shin WC Rhee MH Kim TG Kang JH Kim SH Hong S Kang SY 《Life sciences》2006,79(21):2022-2031
Curcumin has been shown to exhibit anti-inflammatory, antimutagenic, and anticarcinogenic activities. However, the modulatory effect of curcumin on the functional activation of primary microglial cells, brain mononuclear phagocytes causing the neuronal damage, largely remains unknown. The current study examined whether curcumin influenced NO production in rat primary microglia and investigated its underlying signaling pathways. Curcumin decreased NO production in LPS-stimulated microglial cells in a dose-dependent manner, with an IC(50) value of 3.7 microM. It also suppressed both mRNA and protein levels of inducible nitric oxide synthase (iNOS), indicating that this drug may affect iNOS gene expression process. Indeed, curcumin altered biochemical patterns induced by LPS such as phosphorylation of all mitogen-activated protein kinases (MAPKs), and DNA binding activities of nuclear factor-kappaB (NF-kappaB) and activator protein (AP)-1, assessed by reporter gene assay. By analysis of inhibitory features of specific MAPK inhibitors, a series of signaling cascades including c-Jun N-terminal kinase (JNK), p38 and NF-kappaB was found to play a critical role in curcumin-mediated NO inhibition in microglial cells. The current results suggest that curcumin is a promising agent for the prevention and treatment of both NO and microglial cell-mediated neurodegenerative disorders. 相似文献
10.
《Journal of enzyme inhibition and medicinal chemistry》2013,28(4):490-494
The methanolic extract of Dictamnus dasycarpus root barks afforded one new glycosidic quinoline alkaloid, 3-[1β-hydroxy-2-(β-D-glucopyranosyloxy)-ethyl)-4-methoxy-2(1H)-quinolinone (1), together with nine known compounds, preskimmianine (2), 8-methoxy-N-methylflindersine (3), dictamine (4), γ-fagarine (5), halopine (6), skimmianine (7), dictangustine-A (8), iso-γ-fagarine (9), isomaculosidine (10). The isolated alkaloids significantly inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated BV2 cells. Among them, compounds 3 and 7 showed the most potent inhibitory activities on LPS-induced NO production. 相似文献
11.
Yoon JS Jeong EJ Yang H Kim SH Sung SH Kim YC 《Journal of enzyme inhibition and medicinal chemistry》2012,27(4):490-494
The methanolic extract of Dictamnus dasycarpus root barks afforded one new glycosidic quinoline alkaloid, 3-[1β-hydroxy-2-(β-D-glucopyranosyloxy)-ethyl)-4-methoxy-2(1H)-quinolinone (1), together with nine known compounds, preskimmianine (2), 8-methoxy-N-methylflindersine (3), dictamine (4), γ-fagarine (5), halopine (6), skimmianine (7), dictangustine-A (8), iso-γ-fagarine (9), isomaculosidine (10). The isolated alkaloids significantly inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated BV2 cells. Among them, compounds 3 and 7 showed the most potent inhibitory activities on LPS-induced NO production. 相似文献
12.
药囊花茎叶的化学成分研究 总被引:1,自引:1,他引:1
药囊花(Cyphotheca montana Diels)茎叶的乙醇提取物具有抗菌活性,进一步的活性追踪表明活性成分在乙酸乙酯和正丁醇萃取物中,我们从这两种萃取物中共分离鉴定出9个化合物,其结构通过波谱分析等分别鉴定为:槲皮素-3-O-α-L-鼠李糖甙(1),杨梅素-3-O-α-L鼠李糖甙(2),山萘酚-3-(2″,6″-二-O-(E)-p-羟基苯丙烯酰-β-D-吡喃葡萄糖甙(3),3,3′-O-二甲基逆没食子酸-4-O-β-D-吡喃葡萄糖甙(4),槲皮素(5),2α,3β,19α,23-四羧基乌苏-12-烯-28-酸-28-0-β-D吡喃葡萄糖甙(6),乌苏酸(7),β-谷甾醇(8)和胡萝卜甙(9),这些化合物均为首次从我国该特有单种属植物中分离得到。 相似文献
13.
The isolation and identification of sixteen compounds extracted from the aerial parts of A. fruticulosa have been reported in the present study, including eight flavonoids (1, 2, 3, 4, 5, 6, 7 and 8), three terpenoids (9, 10 and 11), two sterols (12 and 13), one lignan (14), one fatty acid (15) and one fatty acid ethyl ester (16), wherein six compounds (2, 3, 4, 5, 10 and 14) have been isolated from A. fruticulosa for the first time. Furthermore, among the identified compounds, three compounds (6, 7 and 11) have also been reported for the first time in the genus Ajanin and three compounds (8, 15 and 16) have not been isolated and reported from other plants of the family Asteraceae. In addition, the chemotaxonomic significance of these compounds was discussed. 相似文献
14.
Ning-Bo Qin Sheng-Ge Li Xue-Yuan Yang Chi Gong Xiang-Yu Zhang Jian Wang Da-Hong Li Yuan-Qiang Guo Zhan-Lin Li Hui-Ming Hua 《Bioorganic & medicinal chemistry letters》2017,27(10):2161-2165
Three new (1–3) and one known (4) bioactive terpenoids were isolated from the seeds of Silybum marianum based on the investigation to get new NO inhibitors. Their structures were determined by extensive NMR (1D and 2D NMR) and MS spectroscopic data, and the absolute configurations were identified by experimental and calculated ECD spectra. The NO inhibitory activities in murine microglial BV-2 cells and interactions with iNOS protein by molecular docking were evaluated for all compounds. The results showed that these compounds had potent NO inhibitory effects. 相似文献
15.
Seung Hyun Kim Sin-Duk Jang Ki Yong Lee Sang Hyun Sung 《Journal of enzyme inhibition and medicinal chemistry》2013,28(1):230-233
A methanolic extract of dried leaves of Polygala japonica Houtt (Polygalaceae) significantly attenuated nitric oxide production in lipopolysaccharide-simulated BV2 microglia. Five anthraquinones chrysophanol (1), emodin (2), aloe-emodin (3), emodin 8-O-β-D-glucopyranoside (4) and trihydroxy anthraquinone (5), and four flavonoids kaempferol (6), chrysoeriol (7), kaempferol 3-gentiobioside (8) and isorhamnetin (9) were isolated from the methanolic extract using bioactivity-guided fractionation. Among them, compounds 1–4, 6 and 7 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglia at the concentrations ranging from 1.0 to 100.0 μM. 相似文献
16.
Chemical Components from Phaeanthus vietnamensis and Their Inhibitory NO Production in BV2 Cells
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Nguyen Xuan Nhiem Nguyen Trung Tuong Pham Thanh Ky Lalita Subedi Seon Ju Park Tran Minh Ngoc Pham Hai Yen Bui Huu Tai Tran Hong Quang Phan Van Kiem Sun Yeou Kim Seung Hyun Kim 《化学与生物多样性》2017,14(8)
Phaeanthus vietnamensis Bân is a well‐known medicinal plant which has been used for the treatment of various inflammatory diseases in traditional medicine. Using various chromatographic methods, three new compounds, (7S,8R,8′R)‐9,9′‐epoxy‐3,5,3′,5′‐tetramethoxylignan‐4,4′,7‐triol ( 1 ), 8α‐hydroxyoplop‐11(12)‐en‐14‐one ( 5 ), and (1R,2S,4S)‐4‐acetyl‐2‐[(E)‐(cinnamoyloxy)]‐1‐methylcyclohexan‐1‐ol ( 12 ) along with twelve known compounds were isolated from the leaves of P. vietnamensis. Their chemical structures were elucidated by physical and chemical methods. All compounds were evaluated for the inhibitory activities of nitric oxide production in LPS‐stimulated BV2 cells. As the results, compound 6 showed the most potent inhibitory activity on LPS‐stimulated NO production in BV2 cells with the IC50 values of 15.7 ± 1.2 μm . Compounds 2 , 7 , and 8 significantly inhibited inflammatory NO production with IC50 values ranging from 22.6 to 25.3 μm . 相似文献
17.
18.
Jia Wang Jing LiuQingfa Wen Congying LiYanfang Li Bing LianHuan Tang Tian Yao 《Biochemical Systematics and Ecology》2010
Juncus setchuensis is a perennial herbaceous plant found in marshes, swamps, and other poorly drained sites. Despite being so abundant in the southwest of China, only one literature reference has reported about its chemical components. The present phytochemical investigation on this plant led to isolations of 13 compounds (including four 9,10-dihydrophenanthrenes, one phenanthrene, one flavonone, two mono-aromatics, one steroid, one anthraquinone and three diterpenoid tanshinones), five of them were isolated form this plant for the first time, four of them, one anthraquinone and three diterpenoid tanshinones, were reported for the first time in this genus. The presence of phenanthrenes, 9,10-dihydrophenanthrenes in the Juncus genus could be of taxonomic importance. 相似文献
19.
《Bioorganic & medicinal chemistry letters》2014,24(12):2717-2719
A new dihydropyranocoumarin, (+)-cis-(3′S,4′S)-diisobutyrylkhellactone (1), together with five known compounds, 3′-senecioyl-4′-acetylkhellactone (2), 3′-isovaleryl-4′-acetylkhellactone (3), 3′,4′-disenecioylkhellactone (4), 3′-isovaleryl-4′-senecioylkhellactone (5), and 3′,4′-diisovalerylkhellactone (6), was isolated from Glehnia littoralis. Their chemical structures were elucidated based on the spectroscopic data interpretation, particularly 1D and 2D NMR data including HMQC and HMBC. All the isolated compounds showed the potential to inhibit LPS-induced nitric oxide production in RAW 264.7 cells with IC50 values ranging from 7.4 to 44.3 μM. 相似文献
20.
A new labdane diterpenoid, leojaponicin (1), a novel norlabdane, methyl 15,16-dinor-7-oxolabda-8-ene-14-oate (2), along with four known labdanes, hispanone (3), leoheteronins A (4) and B (5), 15-methoxyleoheteronin B (6), and three norlabdanes, 14,15,16-trinor-7-oxolabda-8-ene-13-oic acid (7), methyl 14,15,16-trinor-7-oxolabda-8-ene-13-oate (8), 14,15-dinor-8-labdene-7,13-dione (9), and a steroid, stigmast-4-ene-3-one (10), were isolated from a hexane extract of Leonurus japonicus. Their structures were determined using spectroscopic methods, mainly 1-D and 2-D NMR. Compounds 7 and 8 were previously semisynthesized but are reported here for the first time as naturally occurring compounds. In addition, α-glucosidase inhibitory activity of the isolated compounds was evaluated and compound 6 exhibited the strongest effect with IC50 value of 26.7 μM (compared with the positive control acarbose, IC50 = 214.5 μM). 相似文献