共查询到20条相似文献,搜索用时 15 毫秒
1.
Yan G. Zorbas Yuri N. Yaroshenko Nikolai K. Kuznetsov Alexei L. Ivamov 《Biological trace element research》1997,60(1-2):101-113
The objective of this investigation was to evaluate the effect of 47 mg zinc supplementation on deficiency of zinc in rats
during 98 d of restriction of motor activity (hypokinesia), which appeared by higher plasma zinc concentration.
One Hundred 13-week-old Sprague-Dawley male rats weighing 360–390 g were used to perform the studies: They were equally divided
into four groups: 1. Unsupplemented control animals (UCA); 2. Unsupplemented hypokinetic animals (UHA); 3. Supplemented control
animals (SCA); and 4. Supplemented hypokinetic animals (SHA). For the simulation of the effect of hypokinesia (HK), the UHA
and SHA were kept in small individual cages made of wood, which restricted their movements in all directions without hindering
food and water intake. The SCA and SHA received daily with their food an additional amount of zinc.
Before and during the experimental period of 98 d, plasma, urinary and fecal zinc, balance of zinc, food intake, and body
weight were determined at different intervals. In the SHA and UHA, the concentration of zinc in plasma, and the elimination
of zinc in urine and feces increased significantly when compared with the SCA and UCA, whereas the balance of zinc was negative.
The body weight and food intake decreased significantly in the SHA and UHA when compared with the SCA and UCA. The increased
plasma concentration of zinc in both the SHA and UHA groups was in contrast to the observed hypozincnemia during prolonged
immobilization as during prolonged hospitalization. This reaction suggests that there may be some other mechanisms that are
affecting the process of control and regulation of zinc metabolism during prolonged HK.
It was concluded that exposure to prolonged restriction of motor activity of rats induces significant increases in plasma
concentration, fecal and urinary elimination of zinc in the presence of negative zinc balance and regardless the daily intake
of large amounts of zinc with their food, leading to zinc deficiency. 相似文献
2.
Zorbas YG Yaroshenko YY Andreyev VG Kuznetsov NK 《Physiological chemistry and physics and medical NMR》1998,30(2):219-228
The objective of this investigation was to measure the effect of prolonged restriction of motor activity (hypokinesia) of rats on the mass, density, mineral composition, reconstruction parameters and elemental composition of their bone tissue. The studies were done during 90 days of hypokinesia (HK) on 90 male Wistar rats equally divided into two groups: (1) vivarium control rats (VCR) and (2) hypokinetic rats (HKR). For the simulation of the hypokinetic effect the HKR group was kept for 90 days in small individual cages made of wood that restricted the movements of rats in all directions without hindering food and water intakes. During the prehypokinetic period of 15 days and during the hypokinetic period of 90 days bone mass, bone density, bone calcium and phosphorus concentrations, bone reconstruction parameters and elemental composition of bones were determined. During the same periods food intake and body weight losses were also measured. In the HKR group signs of osteoporosis in the spongy structures of the tubular bones were observed; they also showed significant decrease in rat femur weight, and in cross section of the rat femur, and in mineral concentrations of the femoral head when compared with the VCR group. The HKR group also show a significant decrease in food intake and body weight when compared with the VCR group. The corresponding parameters did not change significantly in the VCR group when compared with the baseline control values. It was concluded that prolonged exposure to HK induced osteoporosis and structural changes in bones. This apparently occurred due to inhibition of bone tissue formation in the HKR group. 相似文献
3.
Yan G. Zorbas Nikolai A. Kuznetsov Youri N. Yaroshenko 《Biological trace element research》1997,57(2):169-181
The objective of this investigation was to determine the effect of prolonged restriction of motor activity (hypokinesia [HK])
on several parameters of water metabolism in primates. The studies were performed on 12 rhesus monkeys aged 4–5 yr (5.10–6.85
kg) during the hypokinetic period of 90 d and during the prehypokinetic period of 30 d. They were divided into two equal groups:
the first group was placed under ordinary vivarium conditions (vivarium control animals) and the second group was subjected
to 90 d of HK (hypokinetic animals). For the simulation of the hypokinetic effect, the primates were immobilized on their
abdomens in special tables. The legs of the monkeys were immobilized with hip and knee joints extended. The primates retained
freedom of movement at elbow, wrist, and ankle. During the preexperimental period of 30 d and during the experimental period
of 90 d, the following variables were determined: body weight, total body fluid content, specific total body fluid, mean fluid
consumed and eliminated in urine, specific plasma resistance, hematocrit level, and plasma concentrations of sodium (Na) and
potassium (K). In the hypokinetic primates, body weight decreased significantly when compared to the controls. Mean fluid
intake, total body fluid, and specific total body fluid decreased, whereas mean daily fluid loss and specific mean daily fluid
elimination increased significantly. Specific plasma resistance, hematocrit level, and plasma electrolyte concentrations increased
significantly when compared to the control primates. It was concluded that prolonged restriction of motor activity induces
significant changes in water metabolic parameters of primates leading in decreased total water content of the body. 相似文献
4.
Yan G. Zorbas Andrei G. Kakurin Nikolai K. Kuznetsov Maxim A. Federov Yuri Y. Yaroshenko 《Biological trace element research》1998,63(2):149-166
The aim of this study was to evaluate the effect of magnesium (Mg) loading (10.0 mg Mg/kg body wt) and daily Mg supplements
(5.0 mg Mg/kg body wt) on Mg deficiency shown by increased and not by decreased serum Mg concentration during hypokinesia
(decreased km number/d).
The studies were done during 30 d of prehypokinesia and 364 d of hypokinesia (HK) periods. Forty endurance-trained volunteers
aged 22–26 yr with a peak VO2 max of 66.3 mL·kg−1 min−1 and with an average 15.0 km/d running distance were chose as subjects. They were equally divided into four groups:
The SHKS and SACS groups took daily 5.0 mg elemental Mg/kg body wt and subjected to Mg loading (10.0 mg Mg/kg body wt). Both
the SHKS and UHKS groups were maintained under an average running distance of 4.7 km/d, whereas the SACS and UACS groups did
not experience any modifications to their normal training routines and diets.
During the prehypokinetic and hypokinetic periods, excretion of Mg in feces and urine, concentration of Mg in serum, and Mg
balance were measured. urinary and serum sodium (Na), potassium (K), and calcium (Ca) were also determined. In both SHKS and
UHKS groups, fecal Mg loss, urinary excretion of electrolytes, and serum concentrations of electrolytes increased significantly
(p≤0.05) when compared with the SACS and UACS groups. During Mg loading tests, urinary and fecal Mg excretion was also greater
in the SHKS and UHKS groups than in the SACS and UACS groups. Throughout the study, Mg balance was negative in the SHKS and
UHKS groups, whereas in the SACS and UACS groups, Mg balance was positive.
It was concluded that significant losses of Mg occurred in the presence of negative Mg balance and Mg deficiency in endurance-trained
subjects during prolonged exposure to HK, daily mg supplements, and Mg loading tests. This suggests that Mg is not entering
or being retaining by the bones and cells of many tissues where most Mg is deposited normally, resulting in Mg deficiency
as was shown by the increased serum Mg concentration. 相似文献
1. | Unsupplemented ambulatory control subjects (UACS). |
2. | Unsupplemented hypokinetic subjects (UHKS). |
3. | Supplemented hypokinetic subjects (SHKS). |
4. | Supplemented ambulatory control subjects (SACS). |
5.
Yan G. Zorbas Kirill P. Charapakin Vassil J. Kakurin Nikolai K. Kuznetsov Maxim A. Federov Vladimir K. Popov 《Biological trace element research》1999,69(2):81-98
The aim of this study was to assess the effect of a daily intake of copper supplements on negative copper balance during prolonged
exposure to hypokinesia (decreased number of kilometers per day). During hypokinesia (HK), negative copper balance is shown
by increased, not by decreased, serum copper concentration, as it happens in other situations.
Studies were done during a 30-d prehypokinetic period and a 364-d hypokinetic period. Forty male trained volunteers aged 22–26
yr with a peak oxygen uptake of 66.4 mL/min/kg and with an average of 13.7 km/d running distance were chosen as subjects.
They were equally divided into four groups: unsupplemented ambulatory control subjects (UACS), unsupplemented hypokinetic
subjects (UHKS), supplemented hypokinetic subjects (SHKS), and supplemented ambulatory control subjects (SACS). The SACS and
SHKS groups took 0.09 mg copper carbonate/kg body weight daily. The SHKS and UHKS groups were maintained under an average
running distance of 1.7 km/d, whereas the SACS and UACS groups did not experience any modifications in their normal training
routines.
During the 30-d prehypokinetic period and the 346-d hypokinetic period, urinary excretion of copper, calcium, and magnesium
and serum concentrations of copper, calcium, and magnesium were measured. Copper loss in feces and copper balance was also
determined. In both UHKS and SHKS groups, urinary excretion of copper, calcium, and magnesium and concentrations of copper,
magnesium, and calcium in serum increased significantly when compared with the SACS and UACS groups. Loss of copper in feces
was also increased significantly in the SHKS and UHKS groups when compared with the UACS and SACS groups. Throughout the study,
the copper balance was negative in the SHKS and UHKS groups, whereas in the SACS and UACS groups, the copper balance was positive.
It was concluded that a daily intake of copper supplements cannot be used to prevent copper deficiency shown by increased
copper concentration. Copper supplements also failed to prevent negative copper balance and copper losses in feces and urine
in endurancetrained subjects during prolonged exposure to HK. 相似文献
6.
Tissue magnesium loss during prolonged hypokinesia in magnesium supplemented and unsupplemented rats
Zorbas YG Kakuris KK Denogradov VA Yerullis KB 《Physiological chemistry and physics and medical NMR》2006,38(2):93-104
This study aims at showing that during hypokinesia (HK) tissue magnesium (Mg2+) content decreases more with higher Mg2+ intake than with lower Mg2+ intake and that Mg2+ loss increases more with higher than lower tissue Mg2+ depletion due to inability of the body to use Mg2+ during HK. Studies were conducted on male Wistar rats during a pre-HK period and a HK period. Rats were equally divided into four groups: unsupplemented vivarium control rats (UVCR), unsupplemented hypokinetic rats (UHKR), supplemented vivarium control rats (SVCR) and supplemented hypokinetic rats (SHKR). SVCR and SHKR consumed 42 mEq Mg2+ per day. The gastrocnemius muscle and right femur bone Mg2+ content decreased significantly, while plasma Mg2+ level and urine and fecal Mg2+ loss increased significantly in SHKR and UHKR compared with their pre-HK values and their respective vivarium controls (SVCR and UVCR). However, muscle and bone Mg2+ content decreased more significantly and plasma Mg2+ level, and urine and fecal Mg2+ loss increased more significantly in SHKR than in UHKR. The greater tissue Mg2+ loss with higher Mg2+ intake and the lower tissue Mg2+ loss with lower Mg2+ intake shows that the risk of higher tissue Mg2+ depletion is directly related to the magnitude of Mg2+ intake. The higher Mg2+ loss with higher tissue Mg2+ depletion and the lower Mg2+ loss with lower Mg2+ tissue depletion shows that the risk of greater Mg2+ loss is directly related to the magnitude of tissue Mg2+ depletion. It was concluded that tissue Mg2+ depletion increases more when the Mg2+ intake is higher and that Mg2+ loss increases more with higher than lower tissue Mg2+ depletion indicating that during prolonged HK the tissue Mg2+ depletion is not due to the Mg2+ shortage in food but to the inability of the body to use Mg2+. 相似文献
7.
Yan G. Zorbas Youri F. Federenko Konstantin A. Naexu 《Biological trace element research》1995,50(1):57-78
The aim of this investigation was to evaluate the effect of a daily intake of fluid and salt supplementation on fluid and
electrolyte losses in endurance-trained volunteers during prolonged restriction of muscular activity (hypokinesia). The studies
were performed on 30 long-distance runners aged 23–26 who had a peak oxygen uptake of 65.5 mL/kg/min and had taken 13.8 km/d
on average prior to their participation in the study. The volunteers were divided into three groups: The volunteers in the
first group were placed under normal ambulatory conditions (control subjects), the second group of volunteers subjected to
hypokinesia alone (hypokinetic subjects), and the third group of volunteers was submitted to HK and consumed daily 0.1 g sodium
chloride (NaCl)/kg body wt and 26 mL water/kg body wt (hyperhydrated subjects). The second and third group of volunteers were
kept under an average of 2.7 km/d for 364 d. During the pre-experimental period of 60 d and during the experimental period
of 364 d sodium, potassium, calcium, and magnesium in urine and plasma were determined. Blood was also assayed for osmolality,
hemoglobin, hematocrit, plasma volume, plasma renin activity and plasma aldosterone. Mean arterial blood pressure was also
determined. In the hyperhydrated volunteers plasma volume and arterial blood pressure increased, whereas plasma osmolality,
plasma renin activity, plasma aldosterone, hematocrit, hemoglobin concentration, and urinary excretion and concentrations
of electrolytes in plasma decreased. In the hypokinetic volunteers, plasma volume and arterial blood pressure decreased significantly,
whereas hematocrit values, hemoglobin concenfration, plasma osmolality, plasma renin activity, plasma aldosterone, and electrolytes
in urine and plasma increased significantly during the experimental period. It was concluded that chronic hyperhydration may
be used in minimizing fluid and electrolyte losses in endurance-trained volunteers during prolonged restriction of muscular
activity. 相似文献
8.
Türkan Patiroğlu Gürses Şahin Olgun Kontaş Kazim Üzüm Recep Saraymen 《Biological trace element research》1997,56(2):179-185
In this study, we wanted to examine the effect of magnesium (Mg2+) supplementation on the experimental 3-methyl cholantrene (3-MC)-induced fibrosarcoma and alterations in (Mg2+) distribution in several tissues of the rats, during carcinogenesis. It was determined that serum and tissue (Mg2+) levels of the rats in (Mg2+)-supplemented diet group were higher than those of the rats in the (Mg2+)-nonsupplemented and control groups. The mean time of fibrosarcoma development for (Mg2+)-supplemented group was longer than (Mg2+)-nonsupplemented group (p<0.05). Symptoms of hypermagnesemia were not observed in any of the rats. These results suggests that dietary (Mg2+) supplementation may have a partial anticarcinogenic effect on experimental 3-MC-induced fibrosarcoma by prolongation of the latent period of carcinogenesis. 相似文献
9.
Zorbas YG Popov VK Naexu KA Kakurin VJ Federenko YF 《Physiological chemistry and physics and medical NMR》1998,30(2):229-237
The aim of this study was to examine the structural changes of glomerular and juxta glomerular system (JGS) of the kidney of rats during prolonged restriction of motor activity (hypokinesia). The studies were performed during 90 days of hypokinesia (HK) on 144 male Wistar rats divided into two groups: Group one placed under ordinary vivarium conditions and serving as vivarium control rats (VCR) and Group two subjected to HK and serving as hypokinetic rats (HKR). For the simulation of the hypokinetic effect the HKR group was kept in small individual cages made of wood that restricted the movements of rats in all directions without hindering food and water intake. During a prehypokinetic period of 15 days and the hypokinetic period of 90 days, body weight and food intake were measured and morphometric examinations were done to measure the cortical and juxta medullary glomerulus on kidney sections from the VCR and HKR groups (eight rats from each group). Body weight and food intake decreased significantly in the HKR groups when compared with the VCR group. Kidney weight of rats increased, the superficial volume decreased and that of the juxta medullary glomerules increased, whereas juxta glomerular granularity indexes decreased significantly in the HKR group when compared with the VCR group. The measured parameters did not change significantly in the VCR group when compared with the baseline control values. It was concluded that prolonged exposure to HK induces a significant increase in the kidney weight and a relationship appeared between variations of the volume of cortical and juxta medullary glomerulus and the function of the juxtaglomerular apparatus. 相似文献
10.
11.
12.
The aim of this work is to study the effect of training and Mg supplementation on body pools of Mg and on Mg tissue distribution. Forty male Wistar rats were divided into four groups (n=10): control group (C); trained group (T); Mg-supplemented group (+Mg); and trained and Mg-supplemented group (+MgT). The Mg supplement (100 ppm of Mg) was given in the drinking water for 21 d. The training consisted of swimming during 60% of maximal swimming time obtained in the first session to exhaustion, during 3 wk (5 d a week). The variables measured were: erythrocytes (RBC), hemoglobin (Hb), hematocrit (Hto), total proteins (TP), and Mg in serum, RBC, liver, muscle, bone, and kidney. There was less Mg in liver, muscle, and erythrocyte in trained animals than in control or supplemented animals (T vs C, +MgT vs C and +MgT vs +Mg) (p < 0.01). Trained antimals (T and +MgT) showed higher Mg kidney rates than the untrained ones (p<0.01). There was less bone Mg in control (C) and in supplemented and trained (+MgT) groups than in trained (T) and in supplemented (+Mg) animals (p<0.01). Serum Mg showed a decreasing concentration profile in the following order: +Mg, +MgT, T, C (p<0.01). We conclude that Mg supplementation improves bone and serum Mg levels, but this does not affect Mg status in soft tissues. Maintained exercise leads to a diminution of Mg in the aforementioned soft tissues that is not noticeable in serum, probably provoked by an increase of renal excretion. 相似文献
13.
Yan G. Zorbas Vassil J. Kakurin Victor B. Afonin Nikolai A. Kuznetsov Vladimir L. Yarullin Sergei D. Denogradov 《Biological trace element research》1999,70(1):1-19
Negative potassium balance during hypokinesia (decreased number of kilometers taken/day) is not based on the potassium shortage
in the diet, but on the impossibility of the body to retain potassium. To assess this hypothesis, we study the effect of potassium
loading on athletes during prolonged hypokinesia (HK).
Studies were done during 30 d of a pre-HK period and during 364 d of an HK period. Forty male athletes aged 23–26 yr were
chosen as subjects. They were divided equally into four groups: unloaded ambulatory control subjects (UACS), unloaded hypokinetic
subjects (UHKS), loaded hypokinetic subjects (LHKS), and loaded ambulatory control subjects (LACS). For the simulation of
the hypokinetic effect, the LHKS and UHKS groups were kept under an average running distance of 1.7 km/d. In the LACS and
LHKS groups, potassium loading tests were done by administering 95.35 mg KC1 per kg body weight.
During the pre-HK and HK periods and after KC1 loading tests, fecal and urinary potassium excretion, sodium and chloride excretion,
plasma potassium, sodium and chloride concentration, and potassium balance were measured. Plasma renin activity (PRA) and
plasma aldosterone concentration was also measured. Negative potassium balance increased significantly (p < -0.01) in the UHKS and LHKS groups when compared with the UACS and LACS groups. Plasma electrolyte concentration, urinary
electrolyte excretion, fecal potassium excretion, PRA, and PA concentration increased significantly (p ≤ 0.01) in the LHKS
and UHKS groups when compared with LACS and UACS groups. Urinary and fecal potassium excretion increased much more and much
faster in the LHKS group than in the UHKS group. By contrast, the corresponding parameters change insignificantly in the UACS
and LACS groups when compared with the base line control values.
It was concluded that urinary and fecal potassium excretion increased significantly despite the presence of negative potassium
balance; thus, negative potassium balance may not be based on potassium shortage in the diet because of the impossibility
of the body to retain potassium during HK. 相似文献
14.
Yan G. Zorbas Mitsui A. Sokiguchi Olav A. Johanson Youri F. Federenko 《Biological trace element research》1995,48(2):185-196
The objective of this study was to evaluate the effects of hypokinesia (HK) and fluid- and salt supplementation (FSS) on zinc
metabolism in endurance-trained volunteers (ETV) for a period of 364 d. Thirty long-distance runners aged 22–25 yr with a
peak VO2 of 67 mL/min/kg with an average 13.8 km/d running distance were chosen as subjects. They were equally divided into three
groups:
Throughout the duration of the study, groups 2. and 3. were maintained under an average running distance of 2.7 km/d, whereas
group 1. did not experience any modifications to their normal training routines and diets. Prior to and during the experimental
period, plasma volume, hemoglobin, sodium, potasium, hematocrit, osmolality, and protein concentrations were determined along
with the concentrations and urinary excretions of zinc, magnesium, calcium, and phosphorous. During the HK period, plasma
concentrations of these minerals increased significantly when compared to the HK+FSS and control groups. The same was observed
for the remaining parameters, which led us to conclude that during prolonged restriction of muscular activity, (PRMA) the
body of the HK+FSS volunteers acquire an apparent tendency to retain zinc, whereas in the HK group the opposite is observed. 相似文献
1. | Controls; |
2. | HK subjects; and |
3. | HK+FSS subjects. |
15.
Nitric oxide inhibitor L-NAME when given alone caused a significant rise in both systolic and diastolic pressure, an increase in 24 hr urinary protein excretion and reduction in weight of the litter as compared to control group. Supplementation of MgSO4 at lower dose (250 mg/kg) did not inhibit this pre-eclamptic effect of L-NAME; but in higher doses (500 and 750 mg/kg), it inhibited the pre-eclamptic action of L-NAME. The results suggest that administration of MgSO4 improves the foetal outcome and significantly prevents the development of symptoms of pre-eclampsia like hypertension and proteinuria. 相似文献
16.
This study was designed to investigate the effects of oral zinc and magnesium supplementation on serum thyroid hormone and
lipid levels in alloxan-induced diabetic rats. Thirty-two albino male rats, weighing 234±34 g, were divided into four experimental
groups (control, diabetic, diabetic+zinc supplemented and diabetic+ magnesium supplemented). The experiment lasted for 60
d. The first 45 d of the experiment was the supplementation and last 15 d was the supplementation and diabetes-inducing period.
Diabetic+zinc-supplemented and diabetic+magnesium-supplemented groups were given orally (by adding in their drinking water)
227 mg/L of zinc and 100 mg/kg body weight (bw) of magnesium, respectively throughout the experiment. Control and diabetic
groups served as controls and did not receive zinc or magnesium supplementation. Diabetic, diabetic+zinc-supplemented, and
diabetic+magnesium-supplemented groups were given a daily injection (ip) of 100 mg/kg bw of alloxan for 15 d starting on d
46 of the experiment. The control group was only injected with the same volume of isotonic NaCl as the diabetic group received.
At the end of the of the experiment, rats in all four groups were fasted for 12 h and blood samples were taken from the heart
under ether anesthesia for the determination of thyroid hormone, glucose, total cholesterol, and triglyceride concentrations.
It was found that serum glucose, total cholesterol, and triglyceride concentrations were higher and serum T3 and T4 concentrations
were lower in diabetic rats than those in the control group. Zinc supplementation did not change any parameter in diabetic
rats. However, magnesium supplementation decreased the elevated total cholesterol and triglyceride concentrations of the diabetic
rats to the control level. It was concluded that oral magnesium supplementation might decrease the diabetes-induced disturbances
of lipid metabolism. 相似文献
17.
L I Savinova 《Arkhiv anatomii, gistologii i émbriologii》1985,88(1):71-75
In 56 rabbits kept in tight cages for 4, 8, 12, 16 and 20 weeks, effect of hypokinesia on structure of hemomicrocirculatory bed of the retina has been studied. The retina is digested in tripsin and then it is exfoliated, and the vessels are stained after Shiff. Diameters of arterioles, capillaries and venules are measured, their relations to each other and number of vessels per square unit are taken into consideration. Restriction of the motor activity for 4 weeks results in narrowing of arterioles, capillaries and venules, in increasing venuloarteriolar coefficient, in decreasing number of vessels per square unit, as compared to the norm. In 8 weeks of hypokinesia the diameter of all links of the hemomicrocirculatory bed is evenly increasing, but it does not reach the normal value; the number of the vessels is keeping to decrease; the venuloarteriolar coefficient is at the same level. In 12, 16 and 20 weeks changes in the diameter and the number of the vessels are poorly pronounced, have a wavy character and diversily directed. The value of the venuloarteriolar coefficient is kept constant. As a whole, the data obtained demonstrate that at a long hypokinesia the animals adapt to the new conditions of existence, and the state of the reticular hemomicrocirculatory bed stabilizes. 相似文献
18.
19.
Matsuzaki H Ohdachi J Fuchigami M Masuyama R Uehara M Nakamura K Suzuki K 《Bioscience, biotechnology, and biochemistry》2002,66(1):192-194
To discover the details of the effects of magnesium (Mg) deficiency on kidney function, the course of changes in N-acetyl-beta-D-glucosaminidase (NAG) activity in the urine and in urinary albumin excretion were examined in rats fed a Mg-deficient diet. NAG activity in the urine and urinary albumin excretion in rats fed the Mg-deficient diet significantly increased from 7 d until the end of the feeding period. We suggest that Mg-deficient diet rapidly induces kidney function insufficiency. 相似文献
20.
目的:在大鼠血管钙化模型上,观察外源性补充硫酸镁对大鼠血管钙化的影响,以探讨硫酸镁在血管钙化中作用及机制。方法:用维生素D3加尼古丁诱导大鼠血管钙化,von Kossa染色、钙含量测定及碱性磷酸酶活性测定判断血管钙化程度;用半定量RT-PCR方法测定血管钙化标志分子骨桥蛋白mRNA水平;用生物化学方法测定血管一氧化氮(NO)、超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果:钙化组大鼠血压升高,收缩压较对照组高27%(P<0.05);血管von Kossa染色见血管中膜弹性纤维间可见大量棕黑色颗粒沉积,主动脉钙含量及碱性磷酸酶活性分别较对照高3.9倍和3.4倍(P<0.01),钙化血管组织骨桥蛋白mRNA表达上调40%(P<0.01),血管钙化后可加重血管组织过氧化损伤;而诱导钙化后外源性补充硫酸镁可减轻血管钙化程度,与钙化组比较,低、高剂量硫酸镁组均明显缓解上述指标的变化。结论:诱导血管钙化后外源性补充硫酸镁可以减轻大鼠血管钙化和血管损伤程度。 相似文献