Molecular phylogenetics of Acacia (Fabaceae: Mimosoideae) based on the chloroplast MATK coding sequence and flanking TRNK intron spacer regions

The tribe Acacieae (Fabaceae: Mimosoideae) contains two genera, the monotypic African Faidherbia and the pantropical Acacia, which comprise about 1200 species with over 950 confined to Australia. As currently recognized, the genus Acacia is subdivided into three subgenera: subg. Acacia, subg. Aculeiferum, and the predominantly Australian subg. Phyllodineae. Morphological studies have suggested the tribe Acacieae and genus Acacia are artificial and have a close affinity to the tribe Ingeae. Based on available data there is no consensus on whether Acacia should be subdivided. Sequence analysis of the chloroplast trnK intron, including the matK coding region and flanking noncoding regions, indicate that neither the tribe Acacieae nor the genus Acacia are monophyletic. Two subgenera are monophyletic; section Filicinae of subgenus Aculeiferum does not group with taxa of the subgenus. Section Filicinae, eight Ingeae genera, and Faidherbia form a weakly supported paraphyletic grade with respect to subg. Phyllodineae. Acacia subg. Aculeiferum (s. s.) is sister to the grade. These data suggest that characters currently used to differentiate taxa at the tribal, generic, and subgeneric levels are polymorphic and homoplasious in cladistic analyses.     

A plastid DNA phylogeny of the genus Acacia Miller (Acacieae, Leguminoseae)

Past classifications of the tribe Acacieae Rchb. are outlined and the confusion concerning the relationships of the three subgenera of Acacia Mill. are highlighted. A plastid DNA analysis of Acacieae shows that the genus Acacia is not monophyletic. Furthermore subgenera Acacia Vassal and Aculeiferum Vassal are sister taxa and neither appear closely related to subgenus Phyllodineae (DC.) Ser. Subgenera Acacia and Aculeiferum form a clade that is basal to a well-supported clade consisting of tribe Ingeae Benth. taxa, Faidherbia albida (Del.) A. Chev. and subgenus Phyllodineae. The series of relationships suggested by the cpDNA data contradicts previous investigations of the tribe. Possible explanations of this conflict are explored, and the taxonomic implications of the plastid DNA data set are considered.     

国产黄耆属簇毛黄耆亚属花粉形态及其系统学意义

观察了国产黄耆属簇毛黄耆亚属6组12种植物的花粉形态。这些花粉的外壁表面纹饰可分为网状纹饰,穿孔或小沟渠状,以及穴网状纹饰3种类型。所有种类都具三孔沟的萌发孔,根据内孔明显与否可分为两类。虽然萌发孔类型与分组并没有严格的对应关系,但综合一些花粉特征,仍然发现6个组间花粉形态差异较为明显,与一般的形态差异有一定的对应性。特别是Sect．Robusti比较特殊,根据花粉大小等特征,推测它和Sect.Phyllolobium等有可能是本亚属的原始类群。Sect.Sesbanella的两个近缘种Astragalus heydei和A.hendersonii,花粉形态却很有区别。Sect.Bibacteola种类较多,有24种,花粉形态呈多样性。总之,花粉形态在簇毛亚属中具有一定的系统学意义。     

黄耆属簇毛黄耆亚属系统学研究

簇毛黄耆亚属以其柱头上具簇毛等形态特征与黄耆属其它类群相区别,约含49种（变种）。主要分布在青藏高原及其邻近地区,绝大多数种类分布于横断山区。本文基于形态特征和分支系统学方法分析亚属内种系发生关系。结果表明,49种（变种）可分为9组。其中双小苞组Sect.Bibracteolati含种类最多,是一个并系类群。借助于分支图,分析了6个较重要的鉴定特征的演化,习性,茎的着生方式,小苞片,果实形态,柱头具簇毛,果实膨胀状况等。最后作出了这个亚属的一个分类纲要。     

Phylogenetic analysis of the Brazilian microendemic Paepalanthus subgenus Xeractis (Eriocaulaceae) inferred from morphology

Paepalanthus subgenus Xeractis (Eriocaulaceae) comprises 28 recognized species endemic to the Espinhaço Range, in Minas Gerais state, Brazil. Most species of the subgenus are restricted to small localities and critically endangered, but still in need of systematic study. The monophyly of the subgenus has already been tested, but only with a few species. Our study presents the first phylogenetic hypothesis within the group, based on morphology. A maximum parsimony analysis was conducted on a matrix of 30 characters for 30 terminal taxa, including all species of the subgenus and two outgroups. The biogeographical hypotheses for the subgenus were inferred based on dispersal–vicariance analysis (DIVA). The analysis provided one most‐parsimonious hypothesis that supports most of the latest published subdivisions (sections and series). However, some conflicts remain concerning the position of a few species and the relationships between sections. The distribution and origin(s) of microendemism are also discussed, providing the ground for conservation strategies to be developed in the region. © 2011 The Linnean Society of London, Botanical Journal of the Linnean Society, 2011, 167 , 137–152.     

苔草属复序苔草亚属植物果皮的扫描电镜观察

应用扫描电子显微镜 ,观察了 15种苔草属复序苔草亚属植物果皮的微形态特征。结果表明 ,复序苔草亚属植物果皮的微形态性状多种多样 ,可以为系统学研究提供较丰富的信息 ;并且在所观察的种类中 ,果皮的微形态特征在种内很稳定 ,种间存在不同程度的差异 ,某些近缘种也可表现出一定的相似性。因此 ,上述特征可以做为探讨种之间的分类及亲缘关系的参考。     

梾木属四照花亚属的系统和总览

不同作者对四照花亚属的性状估价不同,因而对其下分类群的分类很不一致。本文据其不同的过冬习性和芽结构与地理分布相关联,建立了两个组、两个系的新系统。并根据"形态地理"学的方法,认为"种"是一组居群,与另一种在形态上需有间断区别,"亚种"是种的组成成分,有一定分布区(若是同域则占居不同生境),形态上有区别,但在分布区交接处有中间类型存在,"变种"是种内限于较狭分布区域成分散分布的变异类形;将该亚属由原来19个种处理成4个种,12个亚种及3个变种。     

Observations of gynoecial structure in Philodendron (Araceae)

MAYO, S. J., 1989. Observations of gynoecial structure in Philodendron (Araceae). The gynoecial morphology and anatomy of 15 species of the genus Philodendron , representing all the subgenera and a majority of the sections, are described and illustrated. The study emphasizes the morphology of the style, compitum and stylar canals; gynoecial vasculature is only superficially treated. Variation of gynoecial morphology in P . subgenus Meconostigma is described and illustrated and a pilot study of gynoecial ontogeny in this subgenus is presented. Ideas on phylogenetic trends in style and stylar canal morphology, ovary locule number, placentation and ovule number are discussed. Gynoecial characters are shown to be taxonomically useful at the subgeneric and sectional level in Philodendron. The following taxonomic and nomenclatural changes are made: P. section Pteromischum is raised to subgeneric rank. In P. subgenus Philodendron sections Polyspermium and Oligospermium are reduced to the synonymy of sections Philodendron and Calostigma respectively, and the following new names and combinations are made at subsectional rank: subsections Canniphyllum, Oligocarpidium, Bulaoanum and Eucardium.     

A Study on the Genus Rhododendron L. Subsection Neriiflora Sleumer in China

The subsection Neriiflora Sleumer is one of the largest subsections of Rhododendron subgenus Hymenanthes, containing 27 species, 8 subspecies and 24 varieties. All the species are divided into four groups according to morphological characters, relationships among the species and their geographical distribution. The evolution and relationships of the group are discussed. The Group D is of highly specialized morphological characters. The subsection has its modern distribution center in the Hengduan Mountains in south-westernChina.     

五味子属（五味子科）分类系统的初步修订

通过对前人提出的五味子属（五味子科）分类系统的点评,根据五味子属植物的33个形态性状及其性状分析,经过分支分析,提出一个新的五味子属分类系统：将五味子属分为五味子亚属和团蕊五味子亚属;将五味子亚属分为多蕊五味子组、少蕊五味子组、中华五味子组和五味子组。编排了分亚属、组和种的检索表,对每个种上分类等级均列出其模式、异名和代表种。     

Morphological phylogenetics of Puya subgenus Puya (Bromeliaceae)

Puya , a large genus mostly from South America, has been taxonomically divided into two subgenera: Puyopsis and Puya. The latter includes only eight species distributed mainly in Chile, extending to Argentina, Bolivia, and Peru. The species of subgenus Puya are recognized by the presence of a sterile apex of the inflorescence branches, whereas those of subgenus Puyopsis have fertile flowers all along the branches. The objectives of this article were to determine whether this diagnostic character was synapomorphic for subgenus Puya , and to explore the relationships between its species. Parsimony analyses were performed for 43 taxa and 93 morphological characters, 87 of which were discrete and six continuous. In the analysis that included all characters, a single most parsimonious tree was found that supported subgenus Puya by two synapomorphic character states, including the diagnostic character of a sterile inflorescence branch apex and a blooming pattern in which flowers open gradually from base to apex. The trees were better supported when the continuous characters were included. Further studies are suggested to resolve the infrageneric classification of Puya and the relationships of the species belonging to subgenus Puya .  © 2008 The Linnean Society of London, Botanical Journal of the Linnean Society , 2008, 156 , 93–110.     

Phylogenetic relationships among bumble bees (Bombus Latr.): a reappraisal of morphological evidence

Abstract. Cladistic analyses of morphological characters of adult bumble bees show significantly higher congruence among characters of the male genitalia than among other characters of males and females. Phylogenetic interpretations of groups supported by the combined morphological evidence (even with minimal assumptions) include (1) that the earliest diverging species belong to the para-phyletic subgenus Mendacibombus , together with Bombus nevadensis and B.confusus ; (2) that, in contrast, species of the subgenus Bombus (sensu stricto) share a relatively recent common ancestor with B.pressus and B.rufipes ; and (3) that Psithyrus (the social parasites) is a monophyletic group, and the sister-group of B.persicus. For genus-group nomenclature a single genus Bombus (sensu lato) is recommended, to include Psithyrus as another subgenus. Names for sections and for groups of subgenera are synonymized with subgeneric names.     

A cladistic study of Arillastrum, Angophora and Eucalyptus (Myrtaceae)

A cladistic study of Anllastrum, Angophora and Eucalyptus (Myrtaceae). Transformed cladistic; character compatibility; branch and bound, and Farris-Wagner methods gave similar solutions in a cladistic study of Arillastrum, Angophora and Eucalyptus. These analyses, based on morphological characters, indicate that Eucalyptus is a monophyletic group and that its sister taxon is Angophora.
Within Eucalyptus , subgenera Blakella and Corymbia are sister taxa to all other groups; subgenera Monocalyptus, Idiogenes and Gaubaea form a monophyletic group with subgenus Monocalyptus sister to subgenera Idiogenes and Gaubaea ; subgenera Symphyomyrtus and Telocalyptus together also form a monophyletic group and, with Eucalyptus similis (subgenus Eudesmia group 4), are sister to the Monocalyptus group. Eucalyptus subgenus Telocalyptus (4 species), Eucalyptus subgenus Idiogenes (1 species) and Eucalyptus subgenus Gaubaea (2 species) should not be recognized as subgenera and some individual species need further examination. Eucalyptus subgenus Eudesmia is a paraphyletic group.
Some characters are identified as parallelisms, e.g. axillary inflorescences, sepaline operculum, bristle glands, and clustered anthers. A more congruent interpretation of the single operculum of Eucalyptus subgenus Monocalyptus as at least partly petaline rather than solely sepaline in origin is suggested.
The area relationships for the taxa are concordant with those derived from geological and climatological information. New Caledonia is sister area to Australia, and within Australia southwestern Australia is sister area to south-eastern and north-eastern Australia.     

Phylogeny of the Pyretophorus Series of Anopheles subgenus Cellia (Diptera: Culicidae)

A cladistic analysis based on morphological characters is presented for the Pyretophorus Series of subgenus Cellia of Anopheles. The cibarial armature was investigated for the first time since the pioneering work of Christophers (1933) , which is still used to define taxonomic series within Cellia, and for the first time ever using scanning electron microscopy. Christophers’ observations of characters of the cibarial armature were corroborated and several novel characters discovered. Despite difficulties in delimiting and encoding morphological characters at the species level, the analysis provided insights into relationships within the Pyretophorus Series. The data support the monophyly of the Series and establish the Oriental species as a monophyletic group. The Afrotropical species are basal and paraphyletic relative to the Oriental species, with Anopheles christyi placed in the most basal position. The pattern of relationships suggests that the capacity to transmit malaria is an ancestral condition subsequently lost independently in several lineages.     

A Study of the Evolutionary Relationships of Fundulus Topminnows (Teleostei: Fundulidae)

A hypothesis of the evolutionary relationships of thirty-fourspecies of Fundulus and their closer relatives is presented.The study is restricted to morphological characters which couldbe determined to be primitive or derived using the methods ofphylogenetic systematics. Following Parenti, a family Fundulidaeis recognized. It is composed of four genera of North Americankillifishes. (Cyprinodontidae as usually constitutedis polyphyletic.)No convincing characters demonstrate that Fundulus is a naturalgroup (a monophyletic group). However, all species of Fundulusshare four derived characters with Lucania. The sister group(closest genealogical relative) of Fundulus plus Lucania isa group composed of the genera Adinia and Leptolucania. Fourmonophyletic groups of Fundulus can be recognized based on sharedderived characters: (1) subgenus Fundulus (seven species excludingthree species placed in Fontinus); (2) subgenus Fonlinus (sixspecies); (3) subgenus Xensima (five species); and (4) subgenusZygonectes (thirteen species). Three species, F. (Plancterus)zebrinus, F. lima, and F. parvipinnis are of uncertain affinitieswithin the Lucania-Fundulus clade     

Cotyledon architecture and anatomy in the Acacieae (Leguminosae: Mimosoideae)

Cotyledon size, shape, venation pattern and anatomy have been investigated in Faidherbia albida and 152 species of Acacia representing the three subgenera Acacia, Aculeiferum and Heterophyllum. Cell volumes of epidermis, palisade and storage tissue, stomatal frequency, stomatal index and frequency of stomatal types have been determined for F. albida and 12 species from each subgenus. The data obtained support the recognition of the subgenera of Acacia as separate taxa but provide no indication of their status. The evidence from cotyledons also supports the separation of Faidherbia from Acacia , and the amalgamation of the Acacieae and Ingeae.     

Pollen morphology and phylogenetic relationships in neotropical Phyllanthus (Euphorbiaceae)

In order to provide new insights into phylogenetic relationships among the neotropical taxa of Phyllanthus , 28 illustrations are provided of the pollen grains of 22 selected species studied from 11 sections of the subgenera represented in the neotropics. Special attention has been given to subgenus Conami because of its variability in pollen morphology: of eight species illustrated, the apertures are diploporate colpi in three species and pores in five species; exine ornamentation is vermiculate in two species and pilate in the other six species. The six species in the neotropical sections Pityrocladus and Microglochidion (subgenus Emblica ) are characterized by prolate grains with an increased number of colpi (4–8). Of particular interest are species in which the pollen exine is clypeate (with exine shields); clypeate pollen grains are illustrated in two species of subgenus Xylophylla and in one species of section Cyclanthera that has unique exine shields with single central pila. The pollen of the one Brazilian phylloclade-bearing species illustrated (in section Choretropsis ) has 3-colporate grains with reticulate exine, typical for subgenus Phyllanthus , and very different from the clypeate grains of the West Indian phylloclade-bearing species in section Xylophylla . This pollen evidence clearly demonstrates homoplasy in the origin of phylloclades in Phyllanthus . Pollen morphological data suggest that the neotropical taxa of Phyllanthus have arisen following colonization from Africa (subgenus Kirganelia ) and Asia (subgenus Emblica ). © 2002 The Linnean Society of London, Botanical Journal of the Linnean Society , 2002, 138 , 325–338.     

Phylogeny and Classification of Prunus sensu lato （Rosaceae）

The classification of the economically important genus Prunus L. sensu lato （s.L） is controversial due to the high levels of convergent or the parallel evolution of morphological characters. In the present study, phylogenetic analyses of fifteen main segregates of Prunus s.I. represented by eighty-four species were conducted with maximum parsimony and Bayesian approaches using twelve chloroplast regions （atpB- rbcL, matK, ndhF, psbA-trnH, rbcL, rpL 16, rpoC1, rps16, trnS-G, trnL, trnL-F and ycfl） and three nuclear genes （ITS, s6pdh and Sbel） to explore their infrageneric used to develop a new, phylogeny-based classification relationships. The results of these analyses were of Prunus s.I. Our phylogenetic reconstructions resolved three main clades of Prunus s.I. with strong supports. We adopted a broad-sensed genus, Prunus, and recognised three subgenera corresponding to the three main clades： subgenus Padus, subgenus Cerasus and subgenus Prunus. Seven sections of subgenus Prunus were recognised. The dwarf cherries, which were previously assigned to subgenus Cerasus, were included in this subgenus Prunus. One new section name, Prunus L. subgenus Prunus section Persicae （T. T. yu ＆ L. T. Lu） S. L. Zhou and one new species name, Prunus tianshanica （Pojarkov） S. Shi, were proposed.     

Phylogenetic relationships among Bactrocera species (Diptera: Tephritidae) inferred from mitochondrial DNA sequences

Several members of the dipteran family Tephritdae are serious pests because females lay eggs in ripening fruit. The genus Bactrocera is one of the largest within the family with over 500 described species arranged in 28 subgenera. The phylogenetic relationships among the various species and subgenera, and the monophyly of specific groups have not been examined using a rigorous phylogenetic analysis. Therefore, phylogenetic relationships among 24 Bactrocera species belonging to 9 subgenera were inferred from DNA sequence of portions of the mitochondrial 16S rRNA, cytochrome oxidase II, tRNA(Lys), and tRNA(Asp) genes. Two morphological characters that traditionally have been used to define the four groups within the subgenus Bactrocera were evaluated in a phylogenetic context by mapping the character states onto the parsimony tree. In addition, the evolutionary trend in male-lure response was evaluated in a phylogenetic context. Maximum parsimony analyses suggested the following relationships: (1) the genus Bactrocera is monophyletic, (2) the subgenus B. (Zeugodacus) is paraphyletic, (3) the subgenus B. (Daculus) is a sister group to subgenus B. (Bactrocera), and (4) the subgenus B. (Bactrocera) is monophyletic. The mapping analyses suggested that the morphological characters exhibit a simple evolutionary transition from one character state to another. Male-lure response was identified as being a labile behavior that has been lost on multiple occasions. Cue-lure response was plesiomorphic to methyl-eugenol response, and the latter has evolved independently within the Bactrocera and Zeugodacus groups of subgenera. The implications of our results for devising a coherent, consolidated classification for Bactrocera is discussed.     

Subpseudocolpi in polyads of Acacia,subgenus Aculeiferum

A revision of the pollen morphology of American species of Acacia, subgenus Aculeiferum, section Monacanthea allowed to characterize, in some species, a new structure: ''subpseudocolpi'' on the distal areas of the grains. Equivalent structures have been found in polyads of some species of Stryphnodendron. The structure is not easily observed under the light microscope, since the reduction of the exine is present only in the infratectal granular layer. It produces an infratectal discontinuity area parallel to the edge of the distal face of the grains, thus allowing folding inwards and outwards of the whole distal polar area delimitated by the ''discontinuity''. As, this feature does not correspond with the concept of pseudocolpus present in subgenus Phyllodinae, which have a reduction of the tectum, a new term: ''subpseudocolpus'', is proposed. The presence of subpseudocolpi (=incipient pseudocolpi) in some species of Stryphnodendron reinforced the pleiophyly hypothesis on the origin of the Acacia considering it to be derived from the Piptadenia group in tropical America.