共查询到20条相似文献,搜索用时 7 毫秒
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L V Miriasova I Ia Moshiashvili I A Basnak'ian V V Sergeev L N Zaporozhtsev 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1978,(6):31-36
The authors studied the antigenic activity of Bordetella pertussis during stab cultivation. For the first time there were revealed changes (oscillations) of antigenic activity: formation of 3 peaks of which the middle was the highest. A possibility of improving the quality of pertussis vaccine by using biomass at the height of the peak was experimentally founded. 相似文献
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E E Sukhinova E I Shmeleva N U Mertsalova I I Iulin V K Golshmid 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1981,(10):64-67
The possibility of optimizing the processes of B. pertussis cultivation carried out in liquid semisynthetic culture media with strong-base anion-exchange resins is shown. The use of strong-base ionites enhances the reproducibility of cultivation processes, increases the activity of microbial populations and the concentration of microorganisms. B. pertussis cultures grown in the media with anion-exchange resins are characterized by pronounced biological activity. 相似文献
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The dynamics of growth ofMycobacterium smegmatis, M. fortuitum andM. phlei in liquid media used also for cultivation of typical mycobacteria (Sauton, Youmans, Kirchner, Šula) was compared with that
in Davis and Merrill media. In the Merrill medium glucose (as the only organic component) was replaced with another carbon
source and the effect of this modification was investigated. The results obtained show that the Merrill medium, its modification
in particular, is suitable for cultivation ofM. smegmatis andM. fortuitum. 2-Oxoglutarate and succinate are important as the sole carbon sources in the case ofM. fortuitum andM. phlei respectively. 相似文献
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ABRAMS RY 《Journal of bacteriology》1955,70(2):251
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Basnak'ian IA Aleksakhina NN Shelemekh OV Miriasova LV Siundiukova RA 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2007,(3):37-42
Bordetella pertussis growth phases during homogenous batch dynamic cultivation in the liquid medium as well as during the static cultivation on the solid medium were established. The maximal activity of agglutination reaction with antisera to B. pertussis agglutinogens 1, 2, and 3 was detected in bacterial culture at the end of exponential phase of growth. The activity of agglutination reaction decreased when cultures in stationary and death phases were used. During transition from exponential to death phase level of antibodies to agglutinogen 2 decreased by4 - 32 times. 2 - 4-fold decrease of antibodies level was observed when antiserum to agglutinogen 3 was used. Activity of agglutination reaction with antiserum to agglutinogen 1 was high and did not depend from phase of growth. When polyvalent antiserum to B. pertussis was used 4-fold decrease of antibody titers was observed in parallel with change of growth phases. Sera from rabbits immunized with B. pertussis cultures from the middle of exponential growth phase, the end of this phase, and begin of the death phase had high (maximal) level of agglutinating antibodies (6400), which was detected on 101 day after immunization with the former culture and on 31 day after immunization with either of the two latter cultures. To the end of experiment (292 day) titers decreased to 800, 3200, and 1600 respectively. These findings confirm an advisability of use of exponential growth culture for immunization of rabbits in order to obtain highly active diagnostic antisera to B. pertussis. 相似文献
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