共查询到20条相似文献,搜索用时 31 毫秒
1.
Panagiota Latsi Panagiotis Pantelidis Dimitris Vassilakis Hiroe Sato Kenneth I Welsh Roland M du Bois 《Respiratory research》2003,4(1):1-5
Background
Genes encoding cytokine mediators are prime candidates for genetic analysis in conditions with T-helper (Th) cell disease driven imbalance. Idiopathic Pulmonary Fibrosis (IPF) is a predominantly Th2 mediated disease associated with a paucity of interferon-gamma (IFN-γ). The paucity of IFN-γ may favor the development of progressive fibrosis in IPF. Interleukin-12 (IL-12) plays a key role in inducing IFN-γ production. The aim of the current study was to assess whether the 1188 (A/C) 3'UTR single nucleotide polymorphism (SNP) in the IL-12 p40 subunit gene which was recently found to be functional and the 5644 (G/A) 3' UTR SNP of the IFN-γ gene were associated with susceptibility to IPF.Methods
We investigated the allelic distribution in these loci in UK white Caucasoid subjects comprising 73 patients with IPF and 157 healthy controls. The SNPs were determined using the polymerase chain reaction in association with sequence-specific primers incorporating mismatches at the 3'-end.Results
Our results showed that these polymorphisms were distributed similarly in the IPF and control groupsConclusion
We conclude that these two potentially important candidate gene single nucleotide polymorphisms are not associated with susceptibility to IPF. 相似文献2.
Alireza Khosravi Gilda Kiani Mehr Roya Kelishadi Shahin Shirani Mojgan Gharipour Aliakbar Tavassoli Fatemeh Noori Nizal Sarrafzadegan 《BMC cardiovascular disorders》2010,10(1):1-8
Background
Acute mental stress may contribute to the cardiovascular disease progression via autonomic nervous system controlled negative effects on the endothelium. The joint effects of stress-induced sympathetic or parasympathetic activity and endothelial function on atherosclerosis development have not been investigated. The present study aims to examine the interactive effect of acute mental stress-induced cardiac reactivity/recovery and endothelial function on the prevalence of carotid atherosclerosis.Methods
Participants were 81 healthy young adults aged 24-39 years. Preclinical atherosclerosis was assessed by carotid intima-media thickness (IMT) and endothelial function was measured as flow-mediated dilatation (FMD) using ultrasound techniques. We also measured heart rate, respiratory sinus arrhythmia (RSA), and pre-ejection period (PEP) in response to the mental arithmetic and speech tasks.Results
We found a significant interaction of FMD and cardiac RSA recovery for IMT (p = 0.037), and a significant interaction of FMD and PEP recovery for IMT (p = 0.006). Among participants with low FMD, slower PEP recovery was related to higher IMT. Among individuals with high FMD, slow RSA recovery predicted higher IMT. No significant interactions of FMD and cardiac reactivity for IMT were found.Conclusions
Cardiac recovery plays a role in atherosclerosis development in persons with high and low FMD. The role of sympathetically mediated cardiac activity seems to be more important in those with impaired FMD, and parasympathetically mediated in those with relatively high FMD. The development of endothelial dysfunction may be one possible mechanism linking slow cardiac recovery and atherosclerosis via autonomic nervous system mediated effect. 相似文献3.
Autoinhibition and soil allelochemical (cyclic dipeptide) levels in replanted Chinese fir (Cunninghamia lanceolata) plantations 总被引:1,自引:0,他引:1
Aims and background
Despite increasing knowledge of the role of allelochemicals in the productivity decline of replanted Chinese fir plantations, relatively little is known about the levels and sources of allelochemicals in relation to autoinhibition.Methods
Allelopathic potential of litter, root exudates, and soils in successive rotations of Chinese fir plantations were detected. An allelochemical cyclic dipeptide (6-hydroxy-1,3-dimethyl-8-nonadecyl-[1,4]-diazocane-2,5-dione) from litter, root exudates, and soils in successive rotations was quantified.Results
Extracts of leaf litter, fine root, and root exudates significantly inhibited the growth of Chinese fir germinants, and inhibition increased with successive rotations. Similar results were observed in the rhizosphere soil, basal soil, and bulk soil. The largest observed inhibition occurred in the rhizosphere soil. Furthermore, cyclic dipeptide was found in litter, root exudates, and soils, and the concentrations increased with successive rotations. The rhizosphere soil had the highest cyclic dipeptide level, followed by basal soil, while bulk soil contained the lowest concentration. There was a significant positive relationship between the inhibition of radicle growth of Chinese fir germinants and the concentration of cyclic dipeptide. Annual release of cyclic dipeptide through root exudation was 2.08–9.78 mol ha?1 annum, but the annual release of cyclic dipeptide through leaf litter decomposition was lowered to 0.32–1.41 mol ha?1 annum.Conclusions
Cyclic dipeptide which caused autoinhibition of Chinese fir may be released into the soil through litter decomposition and root exudation. Root exudates provided more contributions to soil cyclic dipeptide levels than litter in Chinese fir plantations. 相似文献4.
Ngoc P Ly Begona Ruiz-Perez Rachel M McLoughlin Cynthia M Visness Paul K Wallace William W Cruikshank Arthur O Tzianabos George T O'Connor Diane R Gold James E Gern 《Clinical and molecular allergy : CMA》2009,7(1):1-10
Background
Agaricus blazei Murill (AbM) is an edible Brazilian mushroom that has been used in traditional medicine for a range of diseases. It has been shown to have anti-infection and anti-tumor properties in the mouse, which are due to induction of Th1 responses. On the other hand, IgE-mediated allergy is induced by a Th2 response.Objective
Since according to the Th1/Th2 paradigm an increased Th1 response may promote a reduced Th2 response, the aim was to examine whether AbM had anti-allergy effects.Methods
A mouse model for allergy was employed, in which the mice were immunized s.c. with the model allergen ovalbumin (OVA). Additionally, the animals were given a mushroom extract, AndoSan?, mainly (82%) containing AbM, but also Hericium erinaceum (15%) and Grifola frondosa (3%), or PBS p.o. either a day before or 19 days after the immunization. The mice were sacrificed on day 26, and anti-OVA IgE (Th2 response) and IgG2a (Th1 response) antibodies were examined in serum and Th1, Th2 and Treg cytokines in spleen cells cultures.Results
It was found that the AndoSan? extract both when given either before or after OVA immunization reduced the levels of anti-OVA IgE, but not IgG2a, in the mice. There was a tendency to reduced Th2 relative to Th1 cytokine levels in the AndoSan? groups.Conclusion
This particular AbM extract may both prevent allergy development and be used as a therapeutical substance against established allergy. 相似文献5.
Fabiano Reis Priscila Pimentel Collier Thiago Ferreira Souza Guilherme Pinheiro Lopes Eduardo Bronzatto Nivaldo Adolfo Silva Junior Ricardo Mendes Pereira Simone Appenzeller 《Mycopathologia》2013,175(1-2):181-186
Objective
To describe the magnetic resonance imaging (MRI) patterns of the central nervous system (CNS) involvement by neuroparacoccidioidomycosis (NPCM).Methods
Between January 1999 and March 2011, a review of MRI data analysis from 8 cases of NPCM was performed. The following MRI characteristics were examined by an experienced neuroradiologist: topography of lesions, aspects on T1- and T2-weighted images (WI), contrast enhancement, diffusion and spectroscopy.Results
All patients had evidence of paracoccidioidomycosis infection outside the nervous system. Regarding CNS involvement, five patients had only supratentorial lesions; three had infra- and supratentorial ones. Meningeal extension occurred in three patients. The lesions were predominantly hyperintense on T1WI. At T2WI, a hypointense component was present in five cases as well as a perilesional abnormal white matter. A ring-enhancement pattern was seen in five cases. Spectroscopy was performed in three patients and showed an increased lipid peak in all of them. In one case, there was also an increased choline peak.Conclusion
NPCM is rare, and MRI may help its differentiation from other inflammatory lesions. However, the presence of active infection outside CNS and some imaging characteristics should point to this diagnosis. 相似文献6.
Shi D Nakamura T Nakajima M Dai J Qin J Ni H Xu Y Yao C Wei J Liu B Ikegawa S Jiang Q 《Arthritis research & therapy》2008,10(3):R54-6
Introduction
Conflicting findings on the association of single nucleotide polymorphisms (SNPs) in RHOB and TXNDC3 with susceptibility to knee osteoarthritis (OA) have been reported in European Caucasians. To examine the associations of these SNPs with OA in East Asian populations and to evaluate their global significance, we conducted two case-control studies in 955 Chinese and 750 Japanese patients.Methods
We genotyped the previously implicated SNPs rs585017 (in RHOB) and rs4720262 (in TXNDC3) in patients with primary symptomatic knee OA with radiographic confirmation and in matched control individuals, and analyzed their associations. We further conducted a meta-analysis of the study findings together with those of previously reported European studies using the DerSimonian-Laird procedure.Results
A significant association of RHOB with knee OA was observed in male Chinese patients (P = 0.02). No significant associations were found for RHOB in any other comparisons in the East Asian populations. The association of TXNDC3 was replicated in Chinese female (P = 0.04) and Japanese (P = 0.03) patients, although none of these associations persisted after Bonferroni correction. Significant association (P = 0.02 for the allelic frequency) with nonsignificant heterogeneity was found in the East Asian replication study. No significant association was found in any comparison in the meta-analysis for all studies.Conclusion
Our study replicates the association, previously reported in European Caucasians, of TXNDC3 with knee OA susceptibility in an East Asian population. 相似文献7.
Daniel Cadena-Sandoval Isidro Alemán-Ávila Rosa Elda Barbosa-Cobos Lizbeth Teresa Becerril-Mendoza José Manuel Fragoso Julián Ramírez-Bello 《Molecular biology reports》2018,45(3):227-232
Tumor necrosis factor (TNF) plays an important role in the pathogenesis of rheumatoid arthritis (RA). Different genetic variants including the TNF ?308G/A polymorphism are associated with RA susceptibility. However, these findings have not been replicated in all populations. The aim of this study was to determine whether the TNF ?1031T/C (rs1799964), ?376G/A (rs1800750), ?308G/A (rs1800629) ?238G/A (rs361525), and TNFR1 ?609G/T polymorphisms are associated with RA susceptibility in a sample of Mexican patients. Our study included 499 patients with RA and 492 healthy controls. The genotypes of the TNF polymorphisms were obtained using TaqMan assay. The genotype and allele frequencies of the TNF ?1031T/C, ?376G/A, ?308G/A, ?238G/A, and TNFR1 ?609G/T polymorphisms were similar among RA cases versus healthy controls, and no association with RA susceptibility was identified. Our results suggest that the TNF ?1031T/C, ?376G/A, ?308G/A, ?238G/A, and TNFR1 ?609G/T polymorphisms are not associated with RA susceptibility in a sample of Mexican patients. 相似文献
8.
Claudia Durán-Aniotz Gabriela Segal Lorena Salazar Cristián Pereda Cristián Falcón Fabián Tempio Raquel Aguilera Rodrigo González Claudio Pérez Andrés Tittarelli Diego Catalán Bruno Nervi Milton Larrondo Flavio Salazar-Onfray Mercedes N. López 《Cancer immunology, immunotherapy : CII》2013,62(4):761-772
Introduction
Immunization with autologous dendritic cells (DCs) loaded with a heat shock-conditioned allogeneic melanoma cell lysate caused lysate-specific delayed type hypersensitivity (DTH) reactions in a number of patients. These responses correlated with a threefold prolonged long-term survival of DTH+ with respect to DTH? unresponsive patients. Herein, we investigated whether the immunological reactions associated with prolonged survival were related to dissimilar cellular and cytokine responses in blood.Materials and methods
Healthy donors and melanoma patient’s lymphocytes obtained from blood before and after vaccinations and from DTH biopsies were analyzed for T cell population distribution and cytokine release.Results/discussion
Peripheral blood lymphocytes from melanoma patients have an increased proportion of Th3 (CD4+ TGF-β+) regulatory T lymphocytes compared with healthy donors. Notably, DTH+ patients showed a threefold reduction of Th3 cells compared with DTH? patients after DCs vaccine treatment. Furthermore, DCs vaccination resulted in a threefold augment of the proportion of IFN-γ releasing Th1 cells and in a twofold increase of the IL-17-producing Th17 population in DTH+ with respect to DTH? patients. Increased Th1 and Th17 cell populations in both blood and DTH-derived tissues suggest that these profiles may be related to a more effective anti-melanoma response.Conclusions
Our results indicate that increased proinflammatory cytokine profiles are related to detectable immunological responses in vivo (DTH) and to prolonged patient survival. Our study contributes to the understanding of immunological responses produced by DCs vaccines and to the identification of follow-up markers for patient outcome that may allow a closer individual monitoring of patients. 相似文献9.
10.
Background
We examined the association and interaction between maternal viral load and antibodies in vertical transmission of HIV in a non-randomized prospective study of 43 HIV-1 infected pregnant women who attended the San Juan City Hospital, Puerto Rico, and their 45 newborn infants. The women and infants received antiretroviral therapy.Methods
A nested PCR assay of the HIV-1 envelope V3 region and infant PBMC culture were performed to determine HIV status of the infants. Maternal and infant plasma were tested for HIV neutralization or enhancement in monocyte-derived macrophages.Results
Twelve (26.7%) infants were positive by the HIV V3 PCR assay and 3 of the 12 were also positive by culture. There was a trend of agreement between high maternal viral load and HIV transmission by multivariate analysis (OR = 2.5, CI = 0.92, p = 0.0681). Both maternal and infant plasma significantly (p = 0.001 for both) reduced HIV replication at 10-1 dilution compared with HIV negative plasma. Infant plasma neutralized HIV (p = 0.001) at 10-2 dilution but maternal plasma lost neutralizing effect at this dilution. At 10-3 dilution both maternal and infant plasma increased virus replication above that obtained with HIV negative plasma but only the increase by maternal plasma was statistically significant (p = 0.005). There were good agreements in enhancing activity in plasma between mother-infant pairs, but there was no significant association between HIV enhancement by maternal plasma and vertical transmission.Conclusion
Although not statistically significant, the trend of association between maternal viral load and maternal-infant transmission of HIV supports the finding that viral load is a predictor of maternal-infant transmission. Both maternal and infant plasma neutralized HIV at low dilution and enhanced virus replication at high dilution. The antiretroviral treatments that the women received and the small sample size may have contributed to the lack of association between HIV enhancement by maternal plasma and vertical transmission. 相似文献11.
Junliang Xin Baifei Huang Zhongyi Yang Jiangang Yuan Yadong Zhang 《Plant and Soil》2013,372(1-2):431-444
Aims
Mechanisms of low cadmium (Cd) accumulations in cultivars of water spinach are poorly investigated. We aimed to improve understanding of the subcellular biochemical properties of the mechanisms involved.Methods
A pot experiment was conducted to investigate the subcellular distributions of Cd in lateral and main roots, stems, and young and old leaves of a high-Cd (T308) and a low-Cd cultivar (QLQ).Results
The ratio of main root:lateral roots Cd concentration in QLQ was lower (0.34–0.35) than that in T308 (0.39–0.55). The ratio of stem:main root Cd concentration in QLQ was much lower (0.60–0.73) than that in T308 (1.19–1.58). QLQ has higher capacity to sequester Cd in cell wall fractions of main and lateral roots than T308.Conclusions
The difference in shoot Cd concentration between QLQ and T308 is attributable to the difference in Cd translocation from lateral to main roots and from roots to the stem. Fixation of large amounts of Cd in old leaves is beneficial to protect young leaves from Cd toxicity. Cadmium immobilization by the cell wall is important in Cd detoxification, especially in main and lateral roots of QLQ and the shoot of T308. 相似文献12.
Cloning of seed dormancy genes (TaSdr) associated with tolerance to pre-harvest sprouting in common wheat and development of a functional marker 总被引:1,自引:0,他引:1
Yingjun Zhang Xilei Miao Xianchun Xia Zhonghu He 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2014,127(4):855-866
Key message
After cloning and mapping of wheat TaSdr genes, both the functional markers for TaSdr - B1 and TaVp - 1B were validated, and the distribution of allelic variations at TaSdr - B1 locus in the wheat cultivars from 19 countries was characterized.Abstract
Seed dormancy is a major factor associated with pre-harvest sprouting (PHS) in common wheat (Triticum aestivum L.). Wheat TaSdr genes, orthologs of OsSdr4 conferring seed dormancy in rice, were cloned by a comparative genomics approach. They were located on homoeologous group 2 chromosomes, and designated as TaSdr-A1, TaSdr-B1 and TaSdr-D1, respectively. Sequence analysis of TaSdr-B1 revealed a SNP at the position -11 upstream of the initiation codon, with bases A and G in cultivars with low and high germination indices (GI), respectively. A cleaved amplified polymorphism sequence marker Sdr2B was developed based on the SNP, and subsequently functional analysis of TaSdr-B1 was conducted by association and linkage mapping. A QTL for GI co-segregating with Sdr2B explained 6.4, 7.8 and 8.7 % of the phenotypic variances in a RIL population derived from Yangxiaomai/Zhongyou 9507 grown in Shijiazhuang, Beijing and the averaged data from those environments, respectively. Two sets of Chinese wheat cultivars were used for association mapping, and results indicated that TaSdr-B1 was significantly associated with GI. Analysis of the allelic distribution at the TaSdr-B1 locus showed that the frequencies of TaSdr-B1a associated with a lower GI were high in cultivars from Japan, Australia, Argentina, and the Middle and Lower Yangtze Valley Winter Wheat Region and Southwest Winter Wheat Region in China. This study provides not only a reliable functional marker for molecular-assisted selection of PHS in wheat breeding programs, but also gives novel information for a comprehensive understanding of seed dormancy. 相似文献13.
Role of roots in cadmium accumulation of two water spinach cultivars: reciprocal grafting and histochemical experiments 总被引:1,自引:0,他引:1
Junliang Xin Baifei Huang Junzhi Yang Zhongyi Yang Jiangang Yuan Yangxiu Mu 《Plant and Soil》2013,366(1-2):425-432
Background and Aims
Cultivars of water spinach (Ipomoea aquatica Forsk.) differ widely in their shoot cadmium (Cd) concentration. Previously, we suggested that low-Cd cultivars are better able to retain Cd in their roots and thus prevent root-to-shoot Cd translocation. In this study, we explored the roles of roots and shoots in Cd accumulation in a high-Cd (T308) and low-Cd cultivar (QLQ).Methods
We used reciprocal grafting to determine the importance of roots and shoots in Cd accumulation, and a dithizone histochemical method to investigate Cd distribution in the roots.Results
The T308 scion with QLQ rootstock accumulated less Cd than the shoot of non-grafted T308. The QLQ scion with T308 rootstock showed a significantly higher Cd concentration than that in the shoot of non-grafted QLQ. Cadmium induced thicker phellem formation in the main roots of QLQ than in those of T308 and only QLQ showed thickening of the outer cortex cell walls in lateral roots.Conclusions
Shoot Cd accumulation was primarily determined by root-to-shoot Cd translocation, not root Cd uptake. The thicker phellem and outer cortex cell walls in QLQ than in T308 may be one reason why QLQ roots were able to retain more Cd, and thus reducing Cd translocation to shoots. 相似文献14.
Cascão R Moura RA Perpétuo I Canhão H Vieira-Sousa E Mourão AF Rodrigues AM Polido-Pereira J Queiroz MV Rosário HS Souto-Carneiro MM Graca L Fonseca JE 《Arthritis research & therapy》2010,12(5):R196-8
Introduction
Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by sustained synovitis. Recently, several studies have proposed neutrophils and Th17 cells as key players in the onset and perpetuation of this disease. The main goal of this work was to determine whether cytokines driving neutrophil and Th17 activation are dysregulated in very early rheumatoid arthritis patients with less than 6 weeks of disease duration and before treatment (VERA).Methods
Cytokines related to neutrophil and Th17 activation were quantified in the serum of VERA and established RA patients and compared with other very early arthritis (VEA) and healthy controls. Synovial fluid (SF) from RA and osteoarthritis (OA) patients was also analyzed.Results
VERA patients had increased serum levels of cytokines promoting Th17 polarization (IL-1β and IL-6), as well as IL-8 and Th17-derived cytokines (IL-17A and IL-22) known to induce neutrophil-mediated inflammation. In established RA this pattern is more evident within the SF. Early treatment with methotrexate or corticosteroids led to clinical improvement but without an impact on the cytokine pattern.Conclusions
VERA patients already display increased levels of cytokines related with Th17 polarization and neutrophil recruitment and activation, a dysregulation also found in SF of established RA. 0 Thus, our data suggest that a cytokine-milieu favoring Th17 and neutrophil activity is an early event in RA pathogenesis. 相似文献15.
Emanuela Lapice Michele Pinelli Elisabetta Pisu Antonella Monticelli Roberto Gambino Gianfranco Pagano Silvia Valsecchi Sergio Cocozza Gabriele Riccardi Olga Vaccaro 《Cardiovascular diabetology》2010,9(1):1-5
Objective
To evaluate glycemic variability associated with two different premixed insulin analogue formulations when used in a twice-daily regimen.Patients and Methods
Subjects comprised type 2 diabetic patients aged 20-79 years, treated with twice daily premixed insulin or insulin analogue formulations. All subjects were hospitalized for 6 days and randomized to receive either Humalog Mix 25 (Mix 25) or Humalog Mix 50 (Mix 50). They were then crossed over to the other arm between day 3 and day 4 of the study. Continuous glucose monitoring (CGM) was performed on all subjects to examine the differences in glycemic variability.Results
Eleven type 2 diabetic patients were enrolled. No significant difference was found in 24-hour mean glucose values and their SDs, pre-meal glucose values, increases from pre-meal to peak glucose values, or time to peak glucose levels between either group. However, the mean glucose values observed during 0-8 hrs were significantly lower with Mix 25 compared to Mix 50 (128 vs. 147 mg/dL; p = 0.024).Conclusions
The twice-daily Mix 25 regimen provided superior overnight glycemic control compared to the Mix 50 regimen in Japanese patients with type 2 diabetes. However, both twice-daily regimens with either Mix 25 or Mix 50 provided inadequate post-lunch glycemic control.Trial Registration
Current Controlled Trials UMIN000001327 相似文献16.
Lijian Zhang Jinfeng Chen Yang Ke Robert E Mansel Wen G Jiang 《World journal of surgical oncology》2005,3(1):1-12
Background
Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family. Over-expression of PlGF is known to be associated with pathological angiogenesis. This study examined PlGF expression at protein and message levels in non-small cell lung cancer (NSCLC), in which no reports on the significance of PlGF expression is available to date.Patients and methods
We used immunohistochemistry to assess the PlGF protein and correlated PlGF with microvessel density (MVD), as well as clinical outcome in patients with NSCLC tumours (n = 91). In addition, we applied a real time quantitative PCR assay using SYBR Green chemistry to measure PlGF mRNA in normal lung tissues and NSCLC tumours.Results
PlGF was positively stained mainly in cytoplasm of lung cancer cells. High level staining of PlGF was found in 38.5% NSCLC patients. A high level of MVD in NSCLC was found in 42.9% of cases. Tumours with high level and low level PlGF staining had a significantly different MVD (26.69 vs. 20.79, respectively, p = 0.003). Using both univariate and multivariate analyses, PlGF was found to be an independent prognostic factor. Real time PCR analysis revealed that PlGF mRNA was higher in the cancer tissue than normal tissue (0.95 ± 0.19 vs. 0.57 ± 0.24; p < 0.005) and that PlGF mRNA was significant higher in III-IV stage patients than in I-II stage patients (1.03 ± 0.20 vs. 0.80 ± 0.17; p = 0.011).Conclusion
PlGF expression is significantly more in NSCLC tumour tissues than in matched normal tissues. It has a significant positive association with MVD and is an independent factor for NSCLC patients. PlGF may have a pivotal role in NSCLC development and disease progression. 相似文献17.
Aya Kawasaki Satoshi Ito Hiroshi Furukawa Taichi Hayashi Daisuke Goto Isao Matsumoto Makio Kusaoi Jun Ohashi Robert R Graham Kunio Matsuta Timothy W Behrens Shigeto Tohma Yoshinari Takasaki Hiroshi Hashimoto Takayuki Sumida Naoyuki Tsuchiya 《Arthritis research & therapy》2010,12(5):1-7
Introduction
TNFAIP3 interacting protein 1, TNIP1 (ABIN-1) is involved in inhibition of nuclear factor-κB (NF-κB) activation by interacting with TNF alpha-induced protein 3, A20 (TNFAIP3), an established susceptibility gene to systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Recent genome-wide association studies revealed association of TNIP1 with SLE in the Caucasian and Chinese populations. In this study, we investigated whether the association of TNIP1 with SLE was replicated in a Japanese population. In addition, association of TNIP1 with RA was also examined.Methods
A case-control association study was conducted on the TNIP1 single nucleotide polymorphism (SNP) rs7708392 in 364 Japanese SLE patients, 553 RA patients and 513 healthy controls.Results
Association of TNIP1 rs7708392C was replicated in Japanese SLE (allele frequency in SLE: 76.5%, control: 69.9%, P = 0.0022, odds ratio [OR] 1.40, 95% confidence interval [CI] 1.13-1.74). Notably, the risk allele frequency in the healthy controls was considerably greater in Japanese (69.9%) than in Caucasians (24.3%). A tendency of stronger association was observed in the SLE patients with renal disorder (P = 0.00065, OR 1.60 [95%CI 1.22-2.10]) than in all SLE patients (P = 0.0022, OR 1.40 [95%CI 1.13-1.74]). Significant association with RA was not observed, regardless of the carriage of human leukocyte antigen DR β1 (HLA-DRB1) shared epitope. Significant gene-gene interaction between TNIP1 and TNFAIP3 was detected neither in SLE nor RA.Conclusions
Association of TNIP1 with SLE was confirmed in a Japanese population. TNIP1 is a shared SLE susceptibility gene in the Caucasian and Asian populations, but the genetic contribution appeared to be greater in the Japanese and Chinese populations because of the higher risk allele frequency. Taken together with the association of TNFAIP3, these observations underscore the crucial role of NF-κB regulation in the pathogenesis of SLE. 相似文献18.
Carlos A Aguilar-Salinas Andréia Assis-Luores-Vale Benjamín Stockins Hector Mario Rengifo Dondici José Filho Abrahão Afiune Neto Marcílio Lísia Rabelo Kerginaldo Paulo Torres Egídio Paulo de José Oliveira Carlos Alberto Machado Eliana Reyes Victor Saavedra Fernando Florenzano Ma Victoria Hernández Hernandez Sergio Jiménez Erika Ramírez Cuauhtémoc Vazquez Saul Salinas Ismael Hernández Octavio Medel Ricardo Moreno Paula Lugo Ricardo Alvarado Roopa Mehta Victor Gutierrez Francisco J Gómez Pérez 《Cardiovascular diabetology》2004,3(1):1-6
Background
Microalbuminuria and subsequent progression to proteinuria and nephropathy is associated with increased oxidative stress, increased inflammatory cytokines and increased cardiovascular (CVD) risk. The common functional IL-6 -174G>C gene variant is also associated with elevated levels of inflammatory cytokines and CVD risk.Methods
The aim of this study was to examine the association between the IL-6 -174G>C gene variant with plasma total antioxidant status (TAOS) in 552 subjects with type 2 diabetes in relation to urinary protein excretion.Results
In subjects free from CVD, there was a significant interaction between urinary protein excretion (normoalbuminuria/ microalbuminuria/proteinuria) and the -174C allele (compared to -174GG) in determining plasma TAOS (p value for interaction = 0.03). In the -174C allele carriers there was a significant association between plasma TAOS and urinary protein excretion: normalbuminuria v microalbuminuria v proteinuria: 44.30% ± 11.32 vs. 39.74% ± 14.83 vs. 37.93% ± 16.42, ANOVA p = 0.025. In those with CVD, no interaction or association was observed with the -174C allele (p = 0.246).Conclusion
The IL-6 -174G>C gene variant is associated with differences in plasma oxidative stress in response to altered protein excretion in subjects with type 2 diabetes. 相似文献19.
Background
The aetiology of the autoimmune disease type 1 diabetes (T1D) involves many genetic and environmental factors. Evidence suggests that innate immune responses, including the action of interferons, may also play a role in the initiation and/or pathogenic process of autoimmunity. In the present report, we have adopted a linkage disequilibrium (LD) mapping approach to test for an association between T1D and three regions encompassing 13 interferon alpha (IFNA) genes, interferon omega-1 (IFNW1), interferon beta-1 (IFNB1), interferon gamma (IFNG) and the interferon consensus-sequence binding protein 1 (ICSBP1).Results
We identified 238 variants, most, single nucleotide polymorphisms (SNPs), by sequencing IFNA, IFNB1, IFNW1 and ICSBP1, 98 of which where novel when compared to dbSNP build 124. We used polymorphisms identified in the SeattleSNP database for INFG. A set of tag SNPs was selected for each of the interferon and interferon-related genes to test for an association between T1D and this complex gene family. A total of 45 tag SNPs were selected and genotyped in a collection of 472 multiplex families.Conclusion
We have developed informative sets of SNPs for the interferon and interferon related genes. No statistical evidence of a major association between T1D and any of the interferon and interferon related genes tested was found. 相似文献20.