共查询到20条相似文献,搜索用时 0 毫秒
1.
Many blue-light mediated physiological responses have been studied in the fern Adiantum capillus-veneris. We have isolated genomic clones encoding sequences similar to those encoding blue-light photoreceptors (cryptochromes) in
higher plants using the Arabidopsis CRY1 cDNA as a probe, and these positive clones fall into five independent groups. Using RACE procedures, we obtained full-length
cDNA sequences for three of these five groups. The deduced amino acid sequences include the photolyase-homologous domain in
the N-terminal half, and they also contain a C-terminal extension of about 200 amino acids in length. These structural features
indicate that the genes indeed encode Adiantum cryptochromes and represent a small gene family having at least three members.
Received: 16 February 1998 / Accepted: 26 April 1998 相似文献
2.
Plant blue-light receptors 总被引:14,自引:0,他引:14
Lin C 《Trends in plant science》2000,5(8):337-342
Plants have several blue-light receptors, which regulate different aspects of growth and development. Recent studies have identified three such receptors: cryptochrome 1, cryptochrome 2 and phototropin. Cryptochromes 1 and 2 are photolyase-like receptors that regulate hypocotyl growth and flowering time; phototropin mediates phototropism in response to blue light. In addition, phytochrome A has also been found to mediate various blue-light responses. Although the signal-transduction mechanisms of blue-light receptors remain largely unclear, phototropin is probably a protein kinase that regulates cytoplasmic calcium concentrations, whereas the cryptochromes might regulate anion-channel activity and changes in gene expression. 相似文献
3.
To help identify components of the blue-light photoreceptor system for phototropism in Phycomyces blakesleeanus Bgff., proteins from a microsomal fraction obtained from synchronous sporangiophores were studied. By two-dimensional gel electrophoresis, two proteins (FP1, FP2) with covalently bound flavins were found. FP1 has a molecular weight of 71 000 and an isoelectric point of 6.6; FP2 has a molecular weight of 59 000 and an isoelectric point of 6.5. These flavoproteins were purified by column chromatography and gel filtration while assaying for flavins by fluorescence. The relative concentrations of FP1 and FP2 were affected by light applied during growth. These flavoproteins are likely components of the blue-light photoreceptor complex mediating phototropism in Phycomyces.Abbreviations 10 k pellet
10 000-g pellet
- 100 k pellet
100 000-g pellet
- FP1, FP2
proteins with covalently bound flavins having molecular weights of 71 000 and 59 000 and isoelectric points of 6.6 and 6.5, respectively 相似文献
4.
The blue-light sensitive protein YtvA from Bacillus subtilis is built of a photoactive, flavin-binding LOV (Light, Oxygen and Voltage) domain and a STAS domain with unknown function. Here we show that YtvA binds a fluorescent derivative of guanosine triphosphate (GTPTR) that can be displaced by both GTP or ATP. Unspecific NTP (N=G or A) binding is supported by the molecular model of YtvA-STAS. Blue-light activation of YtvA results in small and dark-reversible spectroscopic changes for GTPTR, suggesting that light-driven conformational changes are transmitted from the LOV core to the GTPTR binding site. These results support the idea that STAS domains may have a general NTP binding role and open a way to investigate the molecular functionality of YtvA-STAS. 相似文献
5.
As sessile organisms, plants have evolved a multitude of developmental responses to cope with the ever-changing environmental conditions that challenge the plant throughout its life cycle. Of the many environmental cues that regulate plant development, light is probably the most important. From determining the developmental pattern of the emerging seedling, to influencing the organization of organelles to best maximize energy available for photosynthesis, light has dramatic effects on development during all stages of plant life. In plants, three classes of photoreceptors that mediate light perception have been characterized at the molecular level. The phytochromes recognize light in the red portion of the spectrum, while cryptochromes and phototropins perceive blue and UVA light. In this review, we discuss the different aspects of development that are regulated by these photoreceptors in the model plant species Arabidopsis thaliana and how the phytochromes, cryptochromes, and phototropins bring about changes in development seen in the growing plant. 相似文献
6.
Reorientation of microtubules at the outer epidermal wall of maize coleoptiles by phytochrome,blue-light photoreceptor,and auxin 总被引:3,自引:0,他引:3
Summary The effects of red and blue light on the orientation of cortical microtubules (MTs) underneath the outer epidermal wall of maize (Zea mays L.) coleoptiles were investigated with immunofluorescent techniques. The epidermal cells of dark-grown coleoptiles demonstrated an irregular pattern of regions of parallel MTs with a random distribution of orientations. This pattern could be changed into a uniformly transverse MT alignment with respect to the long cell axis by 1 h of irradiation with red light. This response was transient as the MTs spontaneously shifted into a longitudinal orientation after 1–2 h of continued irradiation. Induction/reversion experiments with short red and far-red light pulses demonstrated the involvement of phytochrome in this response. In contrast to red light, irradiation with blue light induced a stable longitudinal MT alignment which was established within 10 min. The blue-light response could not be affected by subsequent irradiations with red or far-red light indicating the involvement of a separate blue-light photoreceptor which antagonizes the effect of phytochrome. In mixed light treatments with red and blue light, the blue-light photoreceptor always dominated over phytochrome which exhibited an apparently less stable influence on MT orientation. Long-term irradiations with red or blue light up to 6 h did not reveal any rhythmic changes of MT orientation that could be related to the rhythmicity of helicoidal cell-wall structure. Subapical segments isolated from dark-grown coleoptiles maintained a longitudinal MT arrangement even in red light indicating that the responsiveness to phytochrome was lost upon isolation. Conversely auxin induced a transverse MT arrangement in isolated segments even in blue light, indicating that the responsiveness to blue-light photoreceptor was eliminated by the hormone. These complex interactions are discussed in the context of current hypotheses on the functional significance of MT reorientations for cell development.Abbreviations MT
cortical microtubule
- Pr, Pfr
red and far-red absorbing form of phytochrome 相似文献
7.
8.
During sexual differentiation, Chlamydomonas reinhardtii changes its chemotactic behavior in response to ammonium. Just like gamete formation, the change in chemotaxis mode is controlled by the sequential action of two environmental cues, removal of ammonium or nitrate from the medium and light. Thus, vegetative cells and mating incompetent pre-gametes, the latter being generated by nitrogen starvation in the dark, exhibit chemotaxis towards ammonium. Irradiation of pre-gametes results in a loss of chemotaxis and the gaining of mating competence. Incubation of these gametes in the dark resulted in their regaining chemotactic activity; re-illumination again resulted in its loss. Blue light was shown to be most effective in switching-off chemotaxis. RNA-interference strains with reduced levels of the blue-light receptor phototropin showed an attenuated inactivation of chemotaxis that could be partially compensated by the application of higher fluence rates, suggesting that these light responses are mediated by phototropin. The sharing of photoreceptor and signal transduction components as well as similar temporal patterns observed for changes in chemotaxis towards ammonium and gametic differentiation suggest an integration of the signaling pathways that control these two responses.Abbreviations mt Mating type - Phot Phototropin - RNAi RNA interference - TAP Tris–acetate–phosphate (medium) - TAP–N Nitrogen-free TAP (medium) 相似文献
9.
Rafael Garcés Craig L. Chmielewicz Edward D. Lipson 《Molecular & general genetics : MGG》1986,203(2):341-345
Summary We have identified a new locus in Phycomyces blakesleeanus that codes for a flavoprotein designated previously as FP2. According to results from a sexual cross, this locus, flp, maps near the gene madC, a marker for abnormal phototropic sensitivity. The recombination frequency between flp and madC is about 10%. Further, the flp marker is unlinked with the carotene locus carA. Because the flavoprotein FP2 is absent in certain photoreceptor mutants (Pollock et al. 1985 a), it had been proposed as a candidate for the blue-light photoreceptor. We have found, however, that some strains lacking this protein retain normal phototropism. Therefore, the flavoprotein FP2 is probably not involved in photoreception. 相似文献
10.
植物向光性反应的研究进展 总被引:2,自引:0,他引:2
本文对近年来有关植物向光性反应的研究结果作一综述:1)向光素和隐花色素是植物向光反应中的主要光受体,光敏色素在植物向光性反应中也起一定的作用;2)对植物的光辐照度-弯曲度曲线的分析,可知植物的正向光性运动有两种反应,即第一次正向光性弯曲和第二次正向光性弯曲;3)拟南芥(Arabidopsisthaliana)和水稻(Oryza sativa)等植物的根系具有负向光性的特性,根的负向光性倾斜生长角度为负向光性生长和向重性生长相互作用的矢量和;4)生长素的胞间运输依赖于生长素载体,生长素载体的不对称分布和动态运动是生长素极性运输和向性运动的分子基础. 相似文献
11.
12.
Masahiko Ayaki Atsuhiko Hattori Yusuke Maruyama Masaki Nakano Michitaka Yoshimura Momoko Kitazawa 《Chronobiology international》2016,33(1):134-139
We investigated sleep quality and melatonin in 12 adults who wore blue-light shield or control eyewear 2 hours before sleep while using a self-luminous portable device, and assessed visual quality for the two eyewear types. Overnight melatonin secretion was significantly higher after using the blue-light shield (P < 0.05) than with the control eyewear. Sleep efficacy and sleep latency were significantly superior for wearers of the blue-light shield (P < 0.05 for both), and this group reported greater sleepiness during portable device use compared to those using the control eyewear. Participants rated the blue-light shield as providing acceptable visual quality. 相似文献
13.
Nitrogen starvation and blue light are the two environmental cues that control sexual differentiation in Chlamydomonas reinhardtii. Insertional mutagenesis was applied to generate mutants that still require nitrogen starvation as the initiating signal for gametogenesis but were no longer dependent on irradiation. In one mutant analysed, sequences adjacent to the site of insertion were cloned and used for the isolation of a genomic clone that, upon transformation, could complement the mutant phenotype. The gene identified (LRG6) encodes two mRNAs that appear to be the products of differential splicing. The two putative gene products derived from these mRNAs differ in their C-terminal ends. Both predicted gene products exhibit multiple hydrophobic domains with alpha-helical secondary structure typical for integral membrane proteins. These proteins may form pores, and may function as transporters of as-yet unknown substrates. Since rendering the LRG6 gene non-functional resulted in light-independence of gamete formation, it is suggested that this transporter may inhibit signal flux from the photoreceptor to target genes - either directly by its activity or indirectly by serving as a scaffold for signalling proteins. Shutting off this transporter may be required for the activation of signal flux in this pathway. This concept is supported by the observed reduction in LRG6 mRNA levels during the first phase of gametic differentiation. 相似文献
14.
15.
Tissue-specific and subcellular localization of phototropin determined by immuno-blotting 总被引:6,自引:0,他引:6
Phototropin (phot) is a UV/blue- light receptor mediating phototropic reactions of plants as a response to unilateral irradiation. Using an antiserum directed against the N-terminal part of Arabidopsis phot1, we show here cross-reaction with phototropin from Avena sativa, Eruca sativa, Glycine max, Lepidium sativum, Lycopersicon esculentum, Pisum sativum, Sinapis alba, and Zea mays. In all investigated plants, blue light irradiation led to a gel mobility shift of phototropin corresponding to an apparent increase in size of 2–3 kDa. This increase is transient: the apparent size of the phototropin band reverted back to the original size in the dark within 60–90 min. The capacity for in vitro phosphorylation increased to 350% (A. sativa) and 200% (L. sativum) at 90 min after a blue light pulse without an increase in the amount of phototropin protein. Starting from coleoptile tips of monocots that contained the highest concentration of phototropin, we found an exponential decrease in basipetal sections of equal size while a linear decrease was determined for dicots in basipetal sections starting from the section below the hypocotyl hook. We confirmed the membrane association of all phototropin in dark-grown seedlings; after a 2-min blue light pulse, however, 20% of phototropin was found in the cytosolic fraction and only 80% in the membrane fraction. Both fractions showed the gel mobility shift indicating light-dependent autophosphorylation. Detergent-free solubilization of phototropin with chaotropic reagents was investigated with etiolated A. sativa seedlings. Up to 95% of phototropin was solubilized with a mixture of sodium bromide and sodium diphosphate, and subsequently subjected to affinity purification using Cibachron Blue 3GA–agarose as a dinucleotide analogue. Immediately after solubilization, soluble phototropin still showed blue-light-dependent autophosphorylation but lost its activity within less than 1 h.Abbreviations
GFP
Green fluorescent protein
-
phot
Phototropin 相似文献
16.
Loss of a blue-light photoreceptor in the hy4 mutants of Arabidopsis thaliana (L.) Heynh substantially delayed flowering (>100 d to flower vs. 40–50 d), especially with blue light exposure from lamps lacking much red (R) and/or far-red (FR) light. Red night breaks were promotory but flowering was still later for the hy4-101 mutant. However, with exposure to light from FR-rich lamps, flowering of all mutants was early and no different from the wild type. Thus, flowering of Arabidopsis involves a blue-light photoreceptor and other, often more effective photoreceptors. The latter may involve phytochrome photoresponses to R and FR, but with little or no phytochrome response to blue wavelengths.Abbreviations HIR
high irradiance response
- FR
far-red
- R
red
- WT
wild type 相似文献
17.
Side branch formation in the moss, Physcomitrella patens, has been shown to be light dependent with cryptochrome 1a and 1b (Ppcry1a and Ppcry1b), being the blue light receptors for
this response (Imaizumi et al. in Plant Cell 14:373, 2002). In this study, detailed photobiological analyses were performed,
which revealed that this response involves multiple photoreceptors including cryptochromes. For light induction of branches,
blue light of a fluence rate higher than 6 μmol m−2 s−1 for period longer than 3 h is required. The number of branches increased with the increase in fluence rate and in the irradiation
period. The number of branches also increased when red light was applied together with the blue light, although red light
alone had a very few effect. By partially irradiating a cell, both receptive sites for blue and red light were found to be
located around the nucleus. Further, both red and blue light determine the positions of branches being dependent upon the
vibration plane of polarized light. Red light control of branch position was nullified by simultaneous far-red light irradiation.
A blue light effect on branch position was not found in lines with disrupted phototropin genes. Thus, dichroic phytochrome
and phototropin, possibly on the plasma membrane, regulate branch position. These results indicate that at least four distinct
photoreceptor systems, namely, cryptochromes and red light receptor around or in the nucleus, dichroic phytochrome and phototropin
around the cell periphery, are involved in the light induction of side branches in the moss Physcomitrella patens. 相似文献
18.
Hiroko Yatsuhashi 《Journal of plant research》1996,109(2):139-146
Movements of the chloroplasts induced by a directional stimulus of light are found to occur in various plant materials ranging
from algae to terrestrial angiosperms. Depending on the fluence rate of light, chloroplasts move toward the area of the maximum
light absorption and escape from it, which are named as low- and high-fluence-rate responses, respectively. In most materials
the effective wavelengths are exclusively found in the blue-UV region of spectrum, (a) flavin pigment(s) being considered
as the photoreceptor, while in a few species of plants phytochrome is involved. The arrangement of chloroplasts as a result
of the movement depends on the orientation of the electrical vector of light, which reflects the dichroic orientation of the
photoreceptor for perception of light direction. Photosystems involved in these responses, however, are not only for perception
of light direction, but also for realization of the movement and for holding of the chloroplasts in the reached site. Possible
interactions and dual roles of photosystems in regulation of chloroplast movement are discussed. 相似文献
19.
Nitrate uptake in Chlorella saccharophila (Krüger) Nadson was found to be stimulated by blue light, leading to a doubling of the rate. In the presence of background red light (300 mol photons · m-2 · s-1), only 15–20 mol photons · m-2 · s-1 of blue light was sufficient to saturate this increased uptake rate. Incubation of Chlorella cells with anti-nitrate-reductase immunoglobulin-G fragments inhibited blue-light stimulation. However, ferricyanide (10 M) doubled and dithiothreitol (100 M) inhibited the stimulatory effect of blue light. Among the protein-kinase inhibitors used, only staurosporine (10 M) prevented the blue-light stimulation. Phosphatase inhibitors were without effect and sodium vanadate totally inhibited nitrate uptake, pointing to an involvement of the plasma-membrane ATPase. Preincubation of the cells with calmodulin antagonists or calcium ionophores did not significantly reduce blue-light stimulation of nitrate uptake. The data are discussed with regard to transduction of the signal for blue-light stimulation of nitrate uptake and the possibility that the plasma-membrane-bound nitrate reductase is the blue-light receptor.Abbreviations Chl
chlorophyll
- DMSO
dimethylsulfoxide
- 1,2-DHG
1,2-dihexanoylglycerol
- ML-9
1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine
- NR
nitrate reductase
- H-7
1-(5-isoquinolinyl-sulfonyl)-2-methylpiperazine
- IgG
immunoglobulin G
- PFD
photon flux density
- PM
plasma membrane
- W-7
N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide
This work was supported by a grant from the Deutsche Forschungs-gemeinschaft to R.T. 相似文献