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1.
A procedure is described in which large early spermatogonia were isolated from carp testes and purified from an initial 4–5% recovery up to 60–70% using equilibrium density centrifugation on a continuous Percoll gradient. Mice were immunized with the spermatogonia via the intrasplenic route. Six hybridoma cultures, producing monoclonal antibodies (MAbs) reacting selectively with germ cells, were selected and further analysed. Reactivity with five of these MAbs was observed on primordial germ cells (PGCs) in the developing indifferent gonads at the onset of proliferation, i.e. the age of 7 weeks. One MAb, encoded WCG 6, appeared to define a new surface marker on PGCs being gradually expressed on the surface membrane between the age of 2 and 4 weeks, concomitantly with an increase in size of these mitotically silent cells. The reactivity of germ cells with five of the MAbs disappeared completely (WCG 7, 12, 15, 21) or nearly completely (WCG 6) during spermatogenesis, providing a striking difference from patterns obtained with MAbs raised previously against carp spermatozoa. Differences between male and female germ cells were not observed with the WCG-MAbs during gonad development, indicating that a common set of surface antigens is shared between germ cells of both sexes up to and including spermatogonia and oogonia.Abbreviation WCG
Wageningen carp spermatogonia antibody 相似文献
2.
During development of juvenile and young adult carp (Cyprinus carpio, L., Teleostei) three differentiation stages were distinguished in the testis: the prespermatogenic, the early spermatogenic and the advanced spermatogenic testis. Carp testis tissue of these stages was dissociated by enzymatic digestion and viable testis cells with well preserved morphological features were obtained. The surface location and stage-specificity of differentiation antigens on these germ cells was investigated using monoclonal antibodies (MAbs) raised against carp spermatozoa. Binding of MAbs to cells was visualized with immunofluorescence as well as in the immunogold staining assay. Both methods revealed that antigenic determinants defined by seven MAbs were located on the outer surface of testis cells. Four MAbs, i.e. WCS 3, 17, 28 and 29, reacted with germ cells from both pre-spermatogenic testes (WCS 28 weakly) and spermatogenic testes. The antigenic determinants defined by three other MAbs, i.e. WCS 7, 11 and 12, appeared only after the onset of spermatogenesis. In the immunogold staining assay a post-fixation and nuclear staining procedure was developed which allowed identification of isolated germ cells, revealing clearly, for all seven MAbs, that the determinants were expressed on germ cells but not on somatic cells and, for WCS 7, 11 and 12 only, that the determinants first appeared on small spermatogonia prior to meiosis. A survey of the immunogold assay on the binding of the seven MAbs with isolated germ cells from ovaries, is included. 相似文献
3.
Summary During development of juvenile and young adult carp (Cyprinus carpio, L., Teleostei) three differentiation stages were distinguished in the testis: the prespermatogenic, the early spermatogenic and the advanced spermatogenic testis. Carp testis tissue of these stages was dissociated by enzymatic digestion and viable testis cells with well preserved morphological features were obtained. The surface location and stage-specificity of differentiation antigens on these germ cells was investigated using monoclonal antibodies (MAbs) raised against carp spermatozoa. Binding of MAbs to cells was visualized with immunofluorescence as well as in the immunogold staining assay. Both methods revealed that antigenic determinants defined by seven MAbs were located on the outer surface of testis cells. Four MAbs, i.e. WCS 3, 17, 28 and 29, reacted with germ cells from both pre-spermatogenic testes (WCS 28 weakly) and spermatogenic testes. The antigenic determinants defined by three other MAbs, i.e. WCS 7, 11 and 12, appeared only after the onset of spermatogenesis. In the immunogold staining assay a post-fixation and nuclear staining procedure was developed which allowed identification of isolated germ cells, revealing clearly, for all seven MAbs, that the determinants were expressed on germ cells but not on somatic cells and, for WCS 7, 11 and 12 only, that the determinants first appeared on small spermatogonia prior to meiosis. A survey of the immunogold assay on the binding of the seven MAbs with isolated germ cells from ovaries, is included. 相似文献
4.
Satoshi Hamaguchi 《Cell and tissue research》1987,248(2):375-380
Summary The germinal dense body (GDB) in the teleost, Oryzias latipes, an organelle unique to the cells of germ line, is regarded as a counterpart of nuage material in amphibians and mammals. In the study described herein, GDBs in male germ line cells were examined by electron microscopy. GDBs existed continuously in the cytoplasm of primordial germ cells (PGCs), prespermatogonia, type-A spermatogonia and early type-B spermatogonia. But they became rudimentary in late type-B spermatogonia and early spermatocytes, and no longer occurred in spermatids. Differences in the morphology of GDBs of PGCs and male germ cells were also noted. In PGCs of indifferent gonads, about 50% of GDBs were amorphous bodies of fine electron-dense fibrils, whereas in spermatogonia amorphous bodies decreased in number and GDBs of strand-like structure were more frequent. The change in the morphology of GDBs began when the sex differentiation of gonads became evident, and proceeded gradually in prespermatogonia. No obvious differences in morphology of GDBs were noted between prespermatogonia in the fry at later stages of development and spermatogonia in adult fish. 相似文献
5.
S Bruslé-Sicard L Debas B Fourcault J Fuchs 《Reproduction, nutrition, development》1992,32(4):393-406
Sex inversion of Epinephelus microdon, a protogynous hermaphrodite was studied using light and electron microscopic criteria. The sex changes takes place within ex-ovarian lamellae in which, in addition to spermatogonia, numerous primordial germ cells (PGCs) were detected. These undifferentiated and bipotential early germ cells are involved, as well as spermatogonia, in the building up of the testis. 相似文献
6.
In Amphiprion frenatus , a protandric hermaphrodite, male sex inversion is characterized by a decrease of spermatogenic activity in the ovotestis followed by a degeneration of male gevm cells and an increase of oogenic activity. Among female germ cells, undifferentiated primordial germ cells (PGCs) were identified; their participation in building up the ovary is suggested. In addition, the unusual association of juveniles with a single adull member or juvenile groupings lacking the presence of a monogamous pair, induced in juveniles the anticipated sex differentiations (in either male or female orientation) in which not only spermatogonia and oogonia but also PGCs are involved. 相似文献
7.
革胡子鲇原始生殖细胞的起源、迁移及性腺分化 总被引:19,自引:0,他引:19
革胡子鲇又称埃及胡子鲇,是一种多次产卵类型的硬骨鱼。作者用组织学、组织化学、电子显微镜等方法对革胡子鲇的原始生殖细胞(Primordial germ cells,PGCs)的起源、特征、迁移方式和性腺分化进行了研究。实验结果:PGCs来源于内胚层;PGCs的细胞质中存在着一种与生殖细胞有关的电子致密物--生殖质(Germ plasm);PGCs在迁移过程中有主动迁移的能力;PGCs到达生殖嵴的部位后,与生殖上皮细胞(Epithelisl cells)一起共同形成原始性腺;原始性腺分别逐步向精巢和卵巢分化;生殖质与性腺的分化有密切关系;卵巢的分化比精巢早。
相似文献
8.
Spermatogonia in the mouse testis arise from early postnatal gonocytes that are derived from primordial germ cells (PGCs) during embryonic development. The proliferation, self-renewal, and differentiation of spermatogonial stem cells provide the basis for the continuing integrity of spermatogenesis. We previously reported that Pin1-deficient embryos had a profoundly reduced number of PGCs and that Pin1 was critical to ensure appropriate proliferation of PGCs. The current investigation aimed to elucidate the function of Pin1 in postnatal germ cell development by analyzing spermatogenesis in adult Pin1-/- mice. Although Pin1 was ubiquitously expressed in the adult testis, we found it to be most highly expressed in spermatogonia and Sertoli cells. Correspondingly, we show here that Pin1 plays an essential role in maintaining spermatogonia in the adult testis. Germ cells in postnatal Pin1-/- testis were able to initiate and complete spermatogenesis, culminated by production of mature spermatozoa. However, there was a progressive and age-dependent degeneration of the spermatogenic cells in Pin1-/- testis that led to complete germ cell loss by 14 mo of age. This depletion of germ cells was not due to increased cell apoptosis. Rather, detailed analysis of the seminiferous tubules using a germ cell-specific marker revealed that depletion of spermatogonia was the first step in the degenerative process and led to disruption of spermatogenesis, which resulted in eventual tubule degeneration. These results reveal that the presence of Pin1 is required to regulate proliferation and/or cell fate of undifferentiated spermatogonia in the adult mouse testis. 相似文献
9.
Analysis of germ line development in the chick embryo using an anti-mouse EC cell antibody 总被引:1,自引:0,他引:1
L E Urven C A Erickson U K Abbott J R McCarrey 《Development (Cambridge, England)》1988,103(2):299-304
We have found that EMA-1, a monoclonal antibody originally raised against mouse embryonal carcinoma (Nulli SCC1) cells (Hahnel & Eddy, 1982), also labels chick primordial germ cells (PGCs). We have used this antibody in immunohistological studies to follow the development of PGCs in the chick embryo from the time of their initial appearance beneath the epiblast, through their migratory phase and subsequent colonization of the germinal epithelium. During hypoblast formation, individual EMA-1-labelled cells appeared to separate from the basal surface of the epiblast and enter the blastocoel, coincident with the appearance of morphologically identifiable PGCs in this same area. EMA-1 continued to label germ cells until the initiation of gametogenesis in each sex; specifically, labelling was absent by 7-8 days of incubation in females and started to decrease at 11 days of incubation in males. There was a recurrence of the epitope on oogonia at 15 days of incubation, but not on spermatogonia during the remainder of development through hatching. These observations are consistent with an epiblast origin for the avian germ line, and are strikingly similar to those reported for the early mouse embryo using the same antibody (Hahnel & Eddy, 1986). 相似文献
10.
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12.
Chuma S Kanatsu-Shinohara M Inoue K Ogonuki N Miki H Toyokuni S Hosokawa M Nakatsuji N Ogura A Shinohara T 《Development (Cambridge, England)》2005,132(1):117-122
Primordial germ cells (PGCs) are derived from a population of pluripotent epiblast cells in mice. However, little is known about when and how PGCs acquire the capacity to differentiate into functional germ cells, while keeping the potential to derive pluripotent embryonic germ cells and teratocarcinomas. In this investigation, we show that epiblast cells and PGCs can establish colonies of spermatogenesis after transfer into postnatal seminiferous tubules of surrogate infertile mice. Furthermore, we obtained normal fertile offspring by microinsemination using spermatozoa or spermatids derived from PGCs harvested from fetuses as early as 8.5 days post coitum. Thus, fetal male germ cell development is remarkably flexible, and the maturation process, from epiblast cells through PGCs to postnatal spermatogonia, can occur in the postnatal testicular environment. Primordial germ cell transplantation techniques will also provide a novel tool to assess the developmental potential of PGCs, such as those manipulated in vitro or recovered from embryos harboring lethal mutations. 相似文献
13.
Morphological characteristics of male germ cells of rats in contact with Sertoli cells in vitro 总被引:2,自引:0,他引:2
F Palombi E Ziparo F F Rommerts J A Grootegoed M Antonini M Stefanini 《Journal of reproduction and fertility》1979,57(2):325-330
Fragments of seminiferous epithelium were prepared from 3-week-old rats. Although the Sertoli cells formed a monolayer, germ cells (spermatogonia and early spermatocytes) remained in association with them and were of normal ultrastructural appearance. Germ cells became completely separated from Sertoli cells after 3 weeks of culture in a chemically defined medium. The contact areas between Sertoli and germ cells were characterized by desmosome-like junctions while those between germ cells appeared to be pentalaminar. 相似文献
14.
15.
Expression of low density lipoprotein receptor-related protein 4 (Lrp4) gene in the mouse germ cells
Yamaguchi YL Tanaka SS Kasa M Yasuda K Tam PP Matsui Y 《Gene expression patterns : GEP》2006,6(6):607-612
The low density lipoprotein receptor-related protein 4 gene (Lrp4) was identified by subtractive screening of cDNAs of the migratory primordial germ cells (PGCs) of E8.5-9.5 embryo and E3.5 blastocysts. Lrp4 is expressed in PGCs in the hindgut and the dorsal mesentery of E9.5 embryos, and in germ cells in the genital ridges of male and female E10.5-13.5 embryos. Lrp4 is also expressed in spermatogonia of the neonatal and adult testes and in the immature oocytes and follicular cells of the adult ovary. The absence of Lrp4 expression in the blastocyst, embryonic stem cells and embryonic germ cells suggests the Lrp4 is a molecular marker that distinguishes the germ cells from embryo-derived pluripotent stem cells. 相似文献
16.
17.
Liu C Khazanehdari KA Baskar V Saleem S Kinne J Wernery U Chang IK 《Biology of reproduction》2012,86(4):101
The present study aimed to investigate the differentiation of chicken (Gallus gallus domesticus) primordial germ cells (PGCs) in duck (Anas domesticus) gonads. Chimeric ducks were produced by transferring chicken PGCs into duck embryos. Transfer of 200 and 400 PGCs resulted in the detection of a total number of 63.0 ± 54.3 and 116.8 ± 47.1 chicken PGCs in the gonads of 7-day-old duck embryos, respectively. The chimeric rate of ducks prior to hatching was 52.9% and 90.9%, respectively. Chicken germ cells were assessed in the gonad of chimeric ducks with chicken-specific DNA probes. Chicken spermatogonia were detected in the seminiferous tubules of duck testis. Chicken oogonia, primitive and primary follicles, and chicken-derived oocytes were also found in the ovaries of chimeric ducks, indicating that chicken PGCs are able to migrate, proliferate, and differentiate in duck ovaries and participate in the progression of duck ovarian folliculogenesis. Chicken DNA was detected using PCR from the semen of chimeric ducks. A total number of 1057 chicken eggs were laid by Barred Rock hens after they were inseminated with chimeric duck semen, of which four chicken offspring hatched and one chicken embryo did not hatch. Female chimeric ducks were inseminated with chicken semen; however, no fertile eggs were obtained. In conclusion, these results demonstrated that chicken PGCs could interact with duck germinal epithelium and complete spermatogenesis and eventually give rise to functional sperm. The PGC-mediated germline chimera technology may provide a novel system for conserving endangered avian species. 相似文献
18.
Kimura T Nakamura T Murayama K Umehara H Yamano N Watanabe S Taketo MM Nakano T 《Developmental biology》2006,300(2):545-553
Primordial germ cells (PGCs) are germ cell precursors that are committed to sperm or oocytes. Dramatic proliferation during PGC development determines the number of founder spermatogonia and oocytes. Although specified to a germ lineage, PGCs produce pluripotent embryonic germ (EG) cells in vitro and testicular teratomas in vivo. Wnt/beta-catenin signaling regulates pluripotency and differentiation in various stem cell systems, and dysregulation of this signaling causes various human cancers. Here, we examined the role of Wnt/beta-catenin signaling in PGC development. In normal PGC development, Wnt/beta-catenin signaling is suppressed by the GSK3beta-mediated active degradation of beta-catenin and the low expression of canonical Wnt molecules. The effects of aberrant activation of Wnt/beta-catenin signaling in PGCs were analyzed using mice carrying a deletion of the exon that encodes the GSK3beta phosphorylation sites in the beta-catenin locus. Despite the potential activity of Wnt/beta-catenin signaling in stem cell maintenance and carcinogenesis in various cell lineages, teratomas were not induced in the mice expressing the nuclear-localized beta-catenin in PGCs. Instead, the mutant mice showed germ cell deficiency caused by the delayed cell cycle progression of the proliferative phase PGCs. Our results show that the suppression of Wnt/beta-catenin signaling is a prerequisite for the normal development of PGCs. 相似文献
19.
Presumptive Primordial Germ Cells (pPGCs) and PGCs in Tadpoles from UV-irradiated embryos of Xenopus 总被引:1,自引:1,他引:0
Minoru Kotani Yurie Ogiso Ritsuko Ozaki Kohji Ikenishi Katsuji Tsugawa 《Development, growth & differentiation》1994,36(5):457-467
In order to determine whether or not tadpoles that once lacked primordial germ cells (PGCs) in the genital ridges and dorsal mesentery as a result of ultraviolet (UV) irradiation subsequently contained germ cells at more advanced stages of larval development, the numbers of presumptive PGCs or PGCs were carefully examined in Xenopus tadpoles at Nieuwkoop and Faber's stage 35/36–52 that developed normally from UV-irradiated eggs.
No late-appearing germ cells were observed in almost all the UV-irradiated tadpoles examined at stages 49–52. This same population had completely lacked PGCs at about stage 46. Moreover, presumptive PGCs (pPGCs) or cells with granular cytoplasm that reacted with a monoclonal antibody specific for the germ plasm of cleaving Xenopus eggs stayed in the central part of the endoderm cell mass in the irradiated tadpoles at stage 35/36, when the majority of those cells were located in the dorsal part of the endoderm in unirradiated controls. Furthermore, in the irradiated embryos pPGCs were demonstrated to decrease in number with development and eventually to disappear in tadpoles at about stage 40. The results strongly suggest that UV irradiation under the conditions used here totally eliminated germline cells from the irradiated animals. 相似文献
No late-appearing germ cells were observed in almost all the UV-irradiated tadpoles examined at stages 49–52. This same population had completely lacked PGCs at about stage 46. Moreover, presumptive PGCs (pPGCs) or cells with granular cytoplasm that reacted with a monoclonal antibody specific for the germ plasm of cleaving Xenopus eggs stayed in the central part of the endoderm cell mass in the irradiated tadpoles at stage 35/36, when the majority of those cells were located in the dorsal part of the endoderm in unirradiated controls. Furthermore, in the irradiated embryos pPGCs were demonstrated to decrease in number with development and eventually to disappear in tadpoles at about stage 40. The results strongly suggest that UV irradiation under the conditions used here totally eliminated germline cells from the irradiated animals. 相似文献
20.
Puverel S Barrick C Dolci S Coppola V Tessarollo L 《Development (Cambridge, England)》2011,138(12):2511-2521
RanBPM is a recently identified scaffold protein that links and modulates interactions between cell surface receptors and their intracellular signaling pathways. RanBPM has been shown to interact with a variety of functionally unrelated proteins; however, its function remains unclear. Here, we show that RanBPM is essential for normal gonad development as both male and female RanBPM(-/-) mice are sterile. In the mutant testis there was a marked decrease in spermatogonia proliferation during postnatal development. Strikingly, the first wave of spermatogenesis was totally compromised, as seminiferous tubules of homozygous mutant animals were devoid of post-meiotic germ cells. We determined that spermatogenesis was arrested around the late pachytene-diplotene stages of prophase I; surprisingly, without any obvious defect in chromosome synapsis. Interestingly, RanBPM deletion led to a remarkably quick disappearance of all germ cell types at around one month of age, suggesting that spermatogonia stem cells are also affected by the mutation. Moreover, in chimeric mice generated with RanBPM(-/-) embryonic stem cells all mutant germ cells disappeared by 3 weeks of age suggesting that RanBPM is acting in a cell-autonomous way in germ cells. RanBPM homozygous mutant females displayed a premature ovarian failure due to a depletion of the germ cell pool at the end of prophase I, as in males. Taken together, our results highlight a crucial role for RanBPM in mammalian gametogenesis in both genders. 相似文献