共查询到20条相似文献,搜索用时 31 毫秒
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Agarwal P Agarwal PK Nair S Sopory SK Reddy MK 《Molecular genetics and genomics : MGG》2007,277(2):189-198
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DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. 总被引:58,自引:0,他引:58
Yoh Sakuma Qiang Liu Joseph G Dubouzet Hiroshi Abe Kazuo Shinozaki Kazuko Yamaguchi-Shinozaki 《Biochemical and biophysical research communications》2002,290(3):998-1009
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植物中DREBs类转录因子及其在非生物胁迫中的作用 总被引:3,自引:0,他引:3
低温、干旱、高盐等非生物胁迫能够严重影响植物的生长及作物的产量。最近发现了许多调控多种与逆境相关基因表达的转录因子, 其中DREBs类转录因子能够通过与含有DRE/CRT顺式作用元件的抗逆相关基因启动子区相互作用, 进而调控一系列抗逆基因的表达, 使植物品质得到综合改良从而提高植物对非生物胁迫耐受力。文章通过对DREBs的结构、表达调控、作用方式及机理进行总结, 并结合其在植物胁迫信号通路中的作用以及提高转基因植株胁迫耐受性的最新研究成果加以综述, 并对其在农业生产中的应用前景进行展望。 相似文献
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Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression 总被引:19,自引:0,他引:19
Nakashima K Shinwari ZK Sakuma Y Seki M Miura S Shinozaki K Yamaguchi-Shinozaki K 《Plant molecular biology》2000,42(4):657-665
In plants, a cis-acting element, DRE/CRT, is involved in ABA-independent gene expression in response to dehydration and low-temperature stress. To understand signal transduction pathways from perception of the dehydration stress signal to gene expression, we characterized a gene family for DRE/CRT-binding proteins DREB2A and DREB2B in Arabidopsis thaliana. Northern analysis showed that both genes are induced by dehydration and high-salt stress. Organ-specific northern analysis with gene-specific probes showed that these genes are strongly induced in roots by high-salt stress and in stems and roots by dehydration stress. The DREB2A gene is located on chromosome 5, and DREB2B on chromosome 3. We screened an Arabidopsis genomic DNA library with cDNA fragments of DREB2A and DREB2B as probes, and isolated DNA fragments that contained 5-flanking regions of these genes. Sequence analysis showed that both genes are interrupted by a single intron at identical positions in their leader sequence. Several conserved sequences were found in the promoter regions of both genes. The -glucuronidase (GUS) reporter gene driven by the DREB2 promoters was induced by dehydration and high-salt stress in transgenic Arabidopsis plants. 相似文献
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Metabolic Pathways Involved in Cold Acclimation Identified by Integrated Analysis of Metabolites and Transcripts Regulated by DREB1A and DREB2A 总被引:3,自引:0,他引:3
Kyonoshin Maruyama Migiwa Takeda Satoshi Kidokoro Kohji Yamada Yoh Sakuma Kaoru Urano Miki Fujita Kyouko Yoshiwara Satoko Matsukura Yoshihiko Morishita Ryosuke Sasaki Hideyuki Suzuki Kazuki Saito Daisuke Shibata Kazuo Shinozaki Kazuko Yamaguchi-Shinozaki 《Plant physiology》2009,150(4):1972-1980
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Narusaka Y Nakashima K Shinwari ZK Sakuma Y Furihata T Abe H Narusaka M Shinozaki K Yamaguchi-Shinozaki K 《The Plant journal : for cell and molecular biology》2003,34(2):137-148
Many abiotic stress-inducible genes contain two cis-acting elements, namely a dehydration-responsive element (DRE; TACCGACAT) and an ABA-responsive element (ABRE; ACGTGG/TC), in their promoter regions. We precisely analyzed the 120 bp promoter region (-174 to -55) of the Arabidopsis rd29A gene whose expression is induced by dehydration, high-salinity, low-temperature, and abscisic acid (ABA) treatments and whose 120 bp promoter region contains the DRE, DRE/CRT-core motif (A/GCCGAC), and ABRE sequences. Deletion and base substitution analyses of this region showed that the DRE-core motif functions as DRE and that the DRE/DRE-core motif could be a coupling element of ABRE. Gel mobility shift assays revealed that DRE-binding proteins (DREB1s/CBFs and DREB2s) bind to both DRE and the DRE-core motif and that ABRE-binding proteins (AREBs/ABFs) bind to ABRE in the 120 bp promoter region. In addition, transactivation experiments using Arabidopsis leaf protoplasts showed that DREBs and AREBs cumulatively transactivate the expression of a GUS reporter gene fused to the 120 bp promoter region of rd29A. These results indicate that DRE and ABRE are interdependent in the ABA-responsive expression of the rd29A gene in response to ABA in Arabidopsis. 相似文献
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Soybean DREB1/CBF‐type transcription factors function in heat and drought as well as cold stress‐responsive gene expression
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Satoshi Kidokoro Keitaro Watanabe Teppei Ohori Takashi Moriwaki Kyonoshin Maruyama Junya Mizoi Nang Myint Phyu Sin Htwe Yasunari Fujita Sachiko Sekita Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《The Plant journal : for cell and molecular biology》2015,81(3):505-518