共查询到20条相似文献,搜索用时 46 毫秒
1.
《Luminescence》2003,18(3):140-144
It was demonstrated recently that luminescence of a free‐living marine bacterium, Vibrio harveyi, stimulates DNA repair, most probably by activation of the photoreactivation process. Here, we ask whether the stimulation of DNA repair could be an evolutionary drive that ensured maintenance and development of early bacterial luminescent systems. To test this hypothesis, we cultivated V. harveyi lux+ bacteria and luxA mutants in mixed cultures. Initial cultures were mixed to obtain a culture consisting of roughly 50% lux+ cells and 50% luxA mutants. Then bacteria were cultivated for several days and ratio of luminescent to dark bacteria was measured. Under these conditions, luxA mutants became highly predominant within a few days of cultivation. This indicates that, without a selective pressure, the luminescence is a disadvantage for bacteria, perhaps due to consumption of significant portion of cell energy. However, when the same experiments were repeated but cultures were irradiated with low UV doses, luminescent bacteria started to predominate shortly after the irradiation. Therefore, we conclude that stimulation of photoreactivation may be an evolutionary drive for bacterial bioluminescence. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
2.
Manmadhan Kanagasabhapathy Hideaki Sasaki Shinichi Nagata 《World journal of microbiology & biotechnology》2008,24(10):2315-2321
We investigated the diversity of epibiotic bacteria possessing antimicrobial activity isolated from nine species of red algae,
and identified their phylogenetic position. For the isolation of epibiotic bacteria, nine species of red algae, Pachymeniopsis lauceolata, Plocamium telfairiae, Gelidium amansii, Chondrus oncellatus, Grateloupia filicina, Ceramium kondoi, Lomentaria catenata, Schizymenia dubyi and Porphyra yezoensis, were collected from the intertidal zone of Awaji Island, Japan. In total 92 bacteria were collected from the above red algal
species. Primary screening results using disc diffusion assay revealed that 33% of bacteria possess antibacterial activity.
Ten bacteria that showed high antibacterial activity were further studied for their ability to inhibit a set of fouling bacteria,
some luminescent Vibrio and Photobacterium species and a panel of pathogenic bacteria. In general, the inhibitory activities were high against fouling and luminescent
bacteria, while low against various pathogenic bacteria tested. These results suggest that some epibiotic bacteria have adapted
to defend their position in their surface environment through the production of antibacterial metabolites giving defense against
a broad spectrum of bacterial competitors. The phylogenetic analysis using 16 S rRNA sequences identified 7 of the 10 strains
as belonging to the genus Bacillus, and other strains each 1 belonging to genus Microbacterium, Psychrobacter, and Vibrio species. 相似文献
3.
The anatomy of bioluminescent organs and mode of light production in 18 species of pony fish have been investigated using fresh and preserved material. The luminescent systems are similarly arranged in all. Basically, the system consists of a light organ located at the distal end of the esophagus, and a series of abdominal accessory structures positioned in tandem for controlling light intensity and for directing and dispersing the light. Light is produced by numerous symbiotic luminous bacteria in the light organ. A simple classification of the luminescent systems is proposed. The light organs of Leiognathus elongatus and L. rivulatus show marked sexual dimorphism. The bacteria present in the light organs of many pony fishes are easily culturable, but not those from L. elongatus. Electron micrographs of the light organs of L. elongatus and L. rivulatus show the presence of numerous rod-shaped bacteria measuring approximately 0.8 µ x 2.4 µ and 0.8 µ x 7.3 µ, respectively. It is concluded that the light organ of L. elongatus contains another example of a type of non-culturable luminous bacteria that have been found elsewhere. Such bacteria appear to require from the host some special factor for growth and luminescence. 相似文献
4.
S. A. Khrulnova I. V. Manukhov G. B. Zavil’gel’skii 《Russian Journal of Genetics》2011,47(12):1415-1421
A group of luminescent strains of marine bacteria Aliivibrio logei has been isolated (basins of the Okhotsk, White and Bering Seas). Strains A. logei were shown to be psycrophilic bacteria with an optimal growth temperature of approximately 15°C. Bioluminescent characteristics
of strains were studied, and the expression of lux genes was shown to be regulated by the “quorum sensing” system. The A. logei lux operon was cloned in Escherichia coli cells and the structure of this operon and its nucleotide sequence were determined. The structure of A. logei lux operon differs markedly from that in the closely related species of luminescent marine bacteria A. fischeri. In the structure of the A. logei lux operon, the luxI gene is absent in front of luxC, and a fragment containing luxR2-luxI genes is located immediately after luxG gene. Luminescent psycrophilic marine bacteria of A. logei are assumed to be widely distributed in cold waters of northern seas. 相似文献
5.
Summary The luminescent bacteria are logically placed in two genera. The common coccoid and frequently non-motile species placed byBeije-rinck first in his genusPhotobacterium, 1889 under the namePhotobacterium phosphorescens syn.Bacterium phosphorescens Fischer, should be recognized as the type species ofPhotobacterium. Other characters indicate that this genus should be placed in the FamilyPseudomonadaceae Winslowet al.. and should include other straight, rod-shaped, luminescent, polar flagellate bacteria that ferment glucose without, however,
necessarily producing gas (H2 and CO2) as does the type species. The species that have the form of vibrios should be accepted as members of the genusVibrio as suggested by several previous investigators. They have characters much like those ofVibrio comma, the type species of the genusVibrio. 相似文献
6.
Conditions that influence the luminescence of natural and recombinant luminescent bacteria in the presence of blood serum were studied. In general, blood serum quenched the luminescence of the marine Photobacterium phosphoreum and the recombinant Escherichia coli strains harboring the luminescent system genes of Photobacterium leiognathi, but enhanced the luminescence of the soil bacterium Photorhabdus luminescens Zm1 and the recombinant E. coli strain harboring the lux operon of P. luminescens Zm1. The quenching effect of blood serum increased with its concentration and the time and temperature of incubation. The components of blood serum that determine the degree and specificity of its action on bacterial luminescence were identified.__________Translated from Mikrobiologiya, Vol. 74, No. 2, 2005, pp. 191–197.Original Russian Text Copyright © 2005 by Deryabin, Polyakov. 相似文献
7.
8.
Nitrifying bacteria play a key role in the global nitrogen cycle due to their ability to convert reduced nitrogen compounds
(ammonium) to oxidized ones (nitrite and nitrate). Recent investigations based on the methods of molecular ecology revealed
that bacteria are responsible for nitrification in natural ecosystems. At the same time, data on the species composition of
the nitrifiers in soil microbial communities are scarce. Soil samples collected in the forest and steppe areas of European
Russia and the enrichment cultures of nitrifying bacteria isolated from these samples were used for molecular studies of the
diversity of the amoA gene encoding the synthesis of the key enzyme of autotrophic ammonium oxidation. The nitrifying bacteria of the genera Nitrosospira and Nitrosovibrio were found in all the studied soils from natural biocenoses and agrocenoses. 相似文献
9.
Vascular architecture in the bacteriogenic light organ of Euprymna tasmanica (Cephalopoda: Sepiolidae)
下载免费PDF全文
![点击此处可从《Invertebrate Biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Symbiosis between southern dumpling squid, Euprymna tasmanica (Cephalopoda: Sepiolidae), and its luminescent symbiont, the bacterium Vibrio fischeri, provides an experimentally tractable system to examine interactions between the eukaryotic host and its bacterial partner. Luminescence emitted by the symbiotic bacteria provides light for the squid in a behavior termed “counter‐illumination,” which allows the squid to mask its shadow amidst downwelling moonlight. Although this association is beneficial, light generated from the bacteria requires large quantities of oxygen to maintain this energy‐consuming reaction. Therefore, we examined the vascular network within the light organ of juveniles of E. tasmanica with and without V. fischeri. Vessel type, diameter, and location of vessels were measured. Although differences between symbiotic and aposymbiotic squid demonstrated that the presence of V. fischeri does not significantly influence the extent of vascular branching at early stages of symbiotic development, these finding do provide an atlas of blood vessel distribution in the organ. Thus, these results provide a framework to understand how beneficial bacteria influence the development of a eukaryotic closed vascular network and provide insight to the evolutionary developmental dynamics that form during mutualistic interactions. 相似文献
10.
Robert J. Schmidt Van D. Gooch Alfred R. Loeblich J. Woodland Hastings 《Journal of phycology》1978,14(1):5-9
Three of ten cultures of Gonyaulax excavata (Braarud) Balech isolated from the 1972 New England red tide are nonluminescent, Biochemical components of dinoflagellate bioluminescence were not detected in the extracts from these three isolates. Cells of the nonluminescent cultures were identical to those of luminescent cultures as compared by light microscopy, major body plate tabulation, cell size and growth. Both luminescent and nonluminescent cells were toxic as determined by using the mouse bioassay for paralytic shellfish poisoning. All the 122 clones made from one of the luminescent isolates were luminescent suggesting this feature is a stable trait. We conclude that these isolates represent luminescent and luminescent strains of G. excavata. This is the first intraspecific investigation of in vitro bioluminescent components between nonluminescent and luminescent strains of a dinoflagellate. 相似文献
11.
Methane-derived carbonate build-ups and associated microbial communities at cold seeps on the lower Crimean shelf (Black Sea) 总被引:2,自引:0,他引:2
Joachim Reitner Jörn Peckmann Andreas Reimer Gabriela Schumann Volker Thiel 《Facies》2005,51(1-4):66-79
In the euxinic waters of the NW’ Black Sea shelf, tower-like carbonate build-ups up to several metres in height grow at sites of cold methane seepage. These structures are part of an unique microbial ecosystem that shows a considerable biodiversity and a remarkable degree of organization. The accretion of the build-ups is promoted by the growth of centimetre-sized, methane-filled spheres constructed by calcifying microbial mats. Progressive mineralization of these spheres involves the early precipitation of strongly luminescent high-Mg-calcite rich in iron sulphides, and closely interfingered aragonite phases that finally create the stable (mega-) thrombolithic fabric of the towers. Within the microbial mats, microorganisms occur in distinctive spatial arrangements. Major players among the microbial consortia are the archaea groups ANME-1 and ANME-2, Crenarchaeota, and sulphate-reducing bacteria (SRB) of the Desulfosarcina/Desulfobacterium group. The intracellular precipitation of iron sulphides (greigite) by some of these bacteria, growing in close association with ANME-2, suggests iron cycling as an additional biogeochemical pathway involved in the anaerobic oxidation of methane (AOM). 相似文献
12.
Hillel T. Schwartz Igor Antoshechkin Paul W. Sternberg 《Symbiosis (Philadelphia, Pa.)》2011,55(3):111-118
Entomopathogenic nematodes of the genus Heterorhabditis live in symbiosis with pathogenic Photorhabdus bacteria. Heterorhabditis nematodes are entirely dependent on these bacteria for their food source; in return, the nematodes offer the bacteria a way
to infect and kill insects. For their part, Photorhabdus bacteria are lethal to a broad range of insect hosts, to other nematodes, and to other microorganisms, but not to their Heterorhabditis hosts. These nematodes offer the potential to provide a robust experimental system for the in•depth study of a mutually beneficial
symbiotic relationship, with both members of the partnership accessible to molecular and genetic studies. New genomic technologies
offer the possibility for this potential to be realized, and for Heterorhabditis nematodes to become a standard model system for the investigation of host•symbiote relationships. We present a perspective
on the application of these technologies to nematode•bacterial symbiosis and an update on our efforts to sequence three Heterorhabditis species reported at the recent NemaSym meeting. 相似文献
13.
Isolation and characterization of autotrophic, hydrogen-utilizing, perchlorate-reducing bacteria 总被引:2,自引:0,他引:2
Shrout JD Scheetz TE Casavant TL Parkin GF 《Applied microbiology and biotechnology》2005,67(2):261-268
Recent studies have shown that perchlorate (ClO4
–) can be degraded by some pure-culture and mixed-culture bacteria with the addition of hydrogen. This paper describes the isolation of two hydrogen-utilizing perchlorate-degrading bacteria capable of using inorganic carbon for growth. These autotrophic bacteria are within the genus Dechloromonas and are the first Dechloromonas species that are microaerophilic and incapable of growth at atmospheric oxygen concentrations. Dechloromonas sp. JDS5 and Dechloromonas sp. JDS6 are the first perchlorate-degrading autotrophs isolated from a perchlorate-contaminated site. Measured hydrogen thresholds were higher than for other environmentally significant, hydrogen-utilizing, anaerobic bacteria (e.g., halorespirers). The chlorite dismutase activity of these bacteria was greater for autotrophically grown cells than for cells grown heterotrophically on lactate. These bacteria used fumarate as an alternate electron acceptor, which is the first report of growth on an organic electron acceptor by perchlorate-reducing bacteria. 相似文献
14.
G. A. Vydryakova 《Applied Biochemistry and Microbiology》2006,42(4):364-368
The presence of lectins on a cell surface was demonstrated for 70 cultures of luminous bacteria using hemagglutination reactions. It was shown that hemagglutination of luminous bacteria is inhibited by glucose, maltose, fructose, mannose, and N-acetyl-D-glucosamine. The differences in the inhibition of hemagglutination of luminescent and nonluminescent (spontaneous mutants) symbiotic cultures by N-acetyl-D-galactosamine were revealed. The fact that N-acetyl-D-galactosamine inhibits hemagglutination of the luminescent symbiotic bacteria but does not inhibit hemagglutination of the symbiotic cultures lacking luminescence suggests that lectins with N-acetyl-D-galactosamine specificity are possibly involved in the formation and functioning of the symbiosis of luminous bacteria with marine animals possessing luminous organs. 相似文献
15.
Sixteen strains of luminescent bacteria of Vibrio and Photobacterium genera were isolated from water of the Azov and Black seas. Two strains prospective for biotesting were genetically identified
as Vibrio fisheri V-9579 and Vibrio fisheri V-9580 according to Russian Industrial Microorganism Collection (VKMP) classification and accepted for depositing. The isolated
luminescent strains exhibited high individual sensitivity to oil derived products, heavy metal salts, sodium dodecyl sulfate
(SDS) and phenol (up to the maximum concentration limit for fishery impoundments). According to EC50, they are ten times more sensitive to heavy metal salts and potassium dichromate and 2–6 times more sensitive to SDS and
phenol compared to P. phosphoreum (Cohn) Ford and Escherichia coli C600 (pPLS-5) strains. Using Vibrio fisheri VKMP V-9579 and Vibrio fisheri VKMP V-9580 as biosensors, we have shown their high sensitivity and efficacy to marine ecosystem toxicity assessment. 相似文献
16.
N. V. Beloborodov A. S. Khodakova I. T. Bairamov A. Yu. Olenin 《Biochemistry. Biokhimii?a》2009,74(12):1350-1355
In previous studies we demonstrated increased amounts of phenylcarboxylic acids (PCA) in serum of patients with sepsis. This
observation prompted the present study of the ability of the human microbiome bacteria to produce PCA in vitro. PCA were detected in culture media by gas chromatography-mass spectrometry. Increased amounts of phenyllactic and p-hydroxyphenyllactic acids were produced by Klebsiella pneumonia, Escherichia coli, and Staphylococcus aureus. Certain strict anaerobes (bifidobacteria, lactobacteria, eubacteria) have also been found to actively produce these PCA,
but these bacteria are not etiologically linked to sepsis. Thus our results demonstrate the ability of sepsis-related bacteria
to produce PCA and provide experimental support for the theory that the accumulation of PCA in the blood of patients with
sepsis results from microbial degradation of phenylalanine and tyrosine. 相似文献
17.
The lux genes from Photobacterium phosphoreum (NCMB844) have been cloned into Escherichia coli in a plasmid containing the T7-bacteriophage promoter. By specific expression in vivo under the T7 promoter, five structural genes (luxA-E) coding for the fatty acid reductase and luciferase polypeptides were identified as well as a new gene, designated as luxF, which codes for a 26kDa polypeptide. This new gene is located between luxB and luxE and thus disrupts the structural gene order of luxCDABE found in the Vibrio genus. The luxF gene and the protein it codes for have recently been identified in other Photobacterium species and so appears to be widely distributed within this genus. Nucleotide sequencing of the luxF gene has shown it to code for a protein homologous to the luciferase subunits, coded by the luxA and luxB genes. Although this gene is not necessary for light emission in all luminescent bacteria, it must play an essential role in the biochemistry, physiology, or ecology of the luminescent system in species of the Photobacterium genus. 相似文献
18.
The Hsp60 and Hsp70 chaperones contain a number of conserved inserts that are restricted to particular phyla of bacteria.
A one aa insert in the E. coli GroEL and a 21–23 insert in the DnaK proteins are specific for most Gram-negative bacteria. Two other inserts in DnaK are
limited to certain groups of proteobacteria. The requirement of these inserts for cellular growth was examined by carrying
out complementation studies with temperature-sensitive (T
s) mutants of E. coli
groEL or dnaK. Our results demonstrate that deletion or most changes in these inserts completely abolished the complementation ability
of the mutant proteins. Studies with GroEL and DnaK from some other species that either lacked or contained these inserts
also indicated that these inserts are essential for growth of E. coli. The DnaK from some bacteria contains a two aa insert that is not found in E. coli. Introduction of this insert into the E. coli DnaK also led to its inactivation, indicating that these inserts are specific for different groups. We postulate that these
conserved inserts that are localized in loop regions on protein surfaces, are involved in some ancillary functions that are
essential for the groups of bacteria where they are found.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
19.
Predation of bacteria by phagocytic cells was first developed during evolution by environmental amoebae. Many of the core mechanisms used by amoebae to sense, ingest and kill bacteria have also been conserved in specialized phagocytic cells in mammalian organisms. Here we focus on recent results revealing how Dictyostelium discoideum senses and kills non‐pathogenic bacteria. In this model, genetic analysis of intracellular killing of bacteria has revealed a surprisingly complex array of specialized mechanisms. These results raise new questions on these processes, and challenge current models based largely on studies in mammalian phagocytes. In addition, recent studies suggest one additional level on complexity by revealing how Dictyostelium recognizes specifically various bacterial species and strains, and adapts its metabolism to process them. It remains to be seen to what extent mechanisms uncovered in Dictyostelium are also used in mammalian phagocytic cells. 相似文献
20.
The cardinalfishes (Apogonidae) are a diverse clade of small, mostly reef-dwelling fishes, for which a variety of morphological data have not yielded a consistent phylogeny. We use DNA sequence to hypothesize phylogenetic relationships within Apogonidae and among apogonids and other acanthomorph families, to examine patterns of evolution including the distribution of a visceral bioluminescence system. In conformance with previous studies, Apogonidae is placed in a clade with Pempheridae, Kurtidae, Leiognathidae, and Gobioidei. The apogonid genus Pseudamia is recovered outside the remainder of the family, not as sister to the superficially similar genus Gymnapogon. Species sampled from the Caribbean and Western Atlantic (Phaeoptyx, Astrapogon, and some Apogon species) form a clade, as do the larger-bodied Glossamia and Cheilodipterus. Incidence of visceral bioluminescence is found scattered throughout the phylogeny, independently for each group in which it is present. Examination of the fine structure of the visceral bioluminescence system through histology shows that light organs exhibit a range of morphologies, with some composed of complex masses of tubules (Siphamia, Pempheris, Parapriacanthus) and others lacking tubules but containing chambers formed by folds of the visceral epithelium (Acropoma, Archamia, Jaydia, and Rhabdamia). Light organs in Siphamia, Acropoma, Pempheris and Parapriacanthus are distinct from but connected to the gut; those in Archamia, Jaydia, and Rhabdamia are simply portions of the intestinal tract, and are little differentiated from the surrounding tissues. The presence or absence of symbiotic luminescent bacteria does not correlate with light organ structure; the tubular light organs of Siphamia and chambered tubes of Acropoma house bacteria, those in Pempheridae and the other Apogonidae do not. 相似文献